토끼에 있어서 Ethanol 의 투여가 요소 형성에 미치는 영향

허린수 경상대학교 축산진흥연구소 1975 畜産振興硏究所報 Vol.3 No.1

A study in the stimulating effect of ethanol on urea formation in rabbits was carried out. When a given amount of ethanol was administered intraperitoneally, the changes in the concentration of free ammonia, urea nitrogen, ornithine and arginine, and ornithine transcarbamylase activity of plasma were observed. In the group pretreated with ethanol plasma ammonia level was significantly lowered, while the concentration of urea nitrogen, ornithine and arginine, and ornithine transcarbamylase activity of plasma were markedly increased, respectively. In the group administered ammonium acetate following the pretreatment with ethanol plasma ammonia level was lowered rapidly, while the concentration of urea nitrogen, ornithine and arginine, and ornithine transcarbamylase activity were distinctly elevated at the earlier stage; and gradually decreased thereafter showing a tendency to recover to the normal levels. In the group administered ammonium acetate after the pretreatment with normal saline there showed a higher level of ammonia compared to those of ethanol-ammonia group. On the contrary the levels of urea nitrogen, ornithine and arginine, and ornithine transcarbamylase activity of plasma indicated lesser changes than those of ethanol-ammonia group. The auihor came to the conclusion that pretreatment with ethan9l stimulated the function of Area cycle in the rabbits.

흰쥐(rat)에 있어서 철분 및 vitamin E 결핍이 다불포화 지방산 및 그 유도체에 미치는 영향

허린수,김성훈,도재철,김순태,Huh, Rhin-sou,Kim, Sung-hoon,Do, Jae-cheul,Kim, Sun-tae 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.2

This study was designed to elucidate the effects of iron, a well known catalyst of lipid peroxidation, on the contents of phospholipids, unsaturated fatty acids composed in phospholipid molecules and their derivatives, prostaglandins, and the composition changes of fatty acids contained in phospholipids. Iron decreased the contents of phospholipids and its components of unsaturated fatty acids. Catalytic action of iron decreased the composition rates of linoleate and linolenate composed in phospholipid molecules, while that of arachidonate was inclined to increase. The content of arachidonate was increased and that of prostaglandins was decreased without regard to increase the precursor of prostaglandins. It may be concluded that the decreases of prostaglandins and the increase of arachidonate are due to inhibition of the activities of enzyme systems responsible for prostaglandin synthesis by lipid peroxides produced by the catalyst of iron.

혈청및 적혈구 막내 인지질의 직접 비색 정량방법

허린수,김성훈,모기철,박항균 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

A new colorimetric method for determining phospholipid in serum and red blood cell membrane has been presented. The advantages of the present method over previous methods are simple and rapid treatments with reasonable accuracy. The procedure includes the following steps; extraction of total lipid from the sample with lipid extraction solvent, isolation of phospholipids from total lipid with chilled acetone and treatment of phospholipid toluene solution with copper reagent, followed by color development of copper salts of phospholipids with sodium-diethyldithiocarbamate. The recovery and reproducibility of the present method were proven to be 99.41% and 1.97%(coefficient of variability), respectively. By the present method, phospholipid concentrations in serum and red cell membrane of slaughtering cattles were determined to be 59.64㎎/dl and 178.63㎎/dl, and those of slaughtering pigs were 72,00㎎/dl and 190.27㎎/dl, respectively.

혈장유리불포화지방산 ( 血漿遊離不飽和脂肪酸 )의 비색정량법에 대한 개정방법

허린수,주기철,박항균,김영홍 ( Rhin Sou Huh,Kee Chul Ju,Hang Kyun Park,Young Hong Kim ) 한국축산학회 1982 한국축산학회지 Vol.24 No.1

In the former method for determining plasma free unsaturated fatty acids developed by Huh and Kim, it always showed relatively high values. The cause of high values was known to be a contamination of phospholipids in the extract of fatty acids. Some modifications were given. Plasma filtrate extracted with ordinary liped solvent was treated by chilled cetone to eliminate the interfering materials, phospholipids, followed by extraction of fatty acids with the solvent mixture. The defect of the former method could be completed by this modification giving sufficient results.

집쥐와 닭 뇌의 γ-Aminobutyric acid 함량

허린수,Huh, Rhin Sou 대한수의학회 1971 大韓獸醫學會誌 Vol.11 No.1

Current interest in ${\gamma}$-aminobutyric acid (GABA) has arisen from the convergence of several independent line of investigation leading to the demonstration that this and related substances are normal products of brain metabolism and that GABA has an important physiological action upon brain function as well as upon certain peripheral nervous structures. The interest for neurophysiologists has been enhanced by the importance of the discovery for the role of humoral mediator of synaptic transmission or regulator of neuronal activity in the central nervous system, particularly if it may shed some elight upon the nature of central inhibitory processes. In accordance with such an interest and importance, this work was performed in order to standardize the normal content as a preliminary investigation of so-called night active and daytime active animals GABA content in their brains when they are exposed to light and darkness. The method, through which the estimation has made in this work, was paper chromatographic method developed by Maynert and Klingman for the estimation of GABA content in animal tissues. The results obtained are summerized as follows: 1) GABA content in the cerebral cortex of house rat ranged from 90 to $310{\mu}g/gm$ of wet weight. 2) The content of GAGA ranging from 130 to $510{\mu}g/gm$ of wet weight was occurred from midbrain of the rat. 3) GABA content was ranged from 30 to $150 {\mu}g/gm$ of wet weight of the rat cerebellum. 4) The contents of fowl cerebral cortex, midbrain, and cerebellum are estimated as ranging 230-590, 250-620, $50-280{\mu}g/gm$ of wet weight, respectively. As a result, it may be concluded that among three brain tissues of both animals the midbrain is the highest region in GABA content. Fowl brain, on the other side, contains more higher GABA content than the house rat brain does.

血漿 遊離不飽和脂肪酸의 比色定量法

許麟洙,金永洪 慶北大學校 1980 論文集 Vol.29 No.-

There was no method but the specific spectrometry described by Wises and Hansen to determine the content of unsaturated fatty acid, although a variety of methods including acid-base titrimetry and colorimetry were utilized to investigate the content of free fatty acid in plasma. The method developed in this experiment was recognized as new, rapid and simple one for analyzing the plasma unsaturated fatty acid by introducing a combined method of Dole's extraction method of total free fatty acid, the isolation method newly developed here of unsaturated fatty acid from the mixtue and Duncombe's colorimetry to determine the copper salt of fatty acid. The recovery rates of unsaturated fatty acid added were from 100.19 to 102.58 with the mean of 100.98%. The average coefficient of variance from the mean was 3.8% as plasma samples from five groups of rabbit (four each group) were analyzed by the procedure decribed above.

Vitamin E가 철분 투여로 인한 rat의 지질 과산화에 미치는 영향

허린수,김영홍,도재철,최연식,정정원,Huh, Rhin-sou,Kim, Young-hong,Do, Jae-cheul,Choi, Yeon-shik,Cheong, Cheong-won 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.3

This study was designed to investigate the effect of dietary vitamin E on the lipid peroxidation by dietary iron-injected to male rats. Sprague-Dawely strain male rats were divided into three experimental groups, namely control, iron injected and iron-vitamin E injected groups. The control group was fed with normal diet; the iron injected group was given normal diet and while injected intraperitoneally 30mg of ferric hydroxide/100g of body weight 20 times every 3 days. The iron-vitamin E injected group was intraperitoneally administered 30mg of ferric hydroxide/100g of body weight 20 times every 3 days and vitamin E every day with the dose of 5IU(5mg)/100g body weight. All experimental groups were maintained for 60 days with feeding on the respective ratio. The results obtained from this experiment were summarized as following: 1. The net weight gain was significantly decreased by the iron injection, but much increased by the vitamin E injection. 2. The contents of unsaturated fatty acid in phospholipid in liver, kidney, muscle and serum were decreased by the iron injection, but increased by the vitamin E injection. 3. The increment of malondialdehyde contents was induced by the iron overloading, but significantly decreased by the vitamin E injection. Therefore, it is suggested that dietary iron administration to male rats facilitates the lipid peroxidation in vivo and vitamin E has the inhibiting effect on lipip peroxidation process by iron.

Rat에 있어서 탈지사료 및 철분투여가 다불포화 지방산, vitamin E 및 그 유도체에 미치는 영향

허린수,김영홍,김성훈,도재철,이영호,최연식,Huh, Rhin-sou,Kim, Young-hong,Kim, Sung-hoon,Do, Jae-cheul,Lee, Young-ho,Choi, Yeon-shik 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.4

This study was designed to elucidate the effects of administration with dietary iron and fat-free diet on the contents of unsaturated fatty acid in phospholipid molecules, vitamin E contents and malondialdehyde contents in liver, kidney, muscle and testis of the male rats. The rats were divided into 3 experimental groups, namely, control, iron injection and fat-free diet administration groups. The control group was fed with normal diet, iron injection group injected intraperitoneally 20mg of ferric hydroxide/100g of body weight 20 times every 3 days and fat-free diet group administered lipid extraction diet with hexane in normal diet. All experimental groups were maintained for 60 days with feeding on the respective ration. The results obtained were summarized as follows: 1. In the mean contents of unsaturated fatty acid in phospholipid of liver, kidney, muscle and testis among groups, control group was 21.31mg/g, 19.38mg/g, 1.67mg/g, 13.68mg/g, iron injection group was 13.83mg/g, 16.53mg/g, 0.71mg/g, 10.11mg/g and fat-free diet group was 21.07mg/g, 19.38mg/g, 1.49mg/g and 13.40mg/g, respectively. 2. In the mean contents of vitamin E in liver, kidney, muscle and testis among groups, control group was 6.77mg/g, 1.93mg/g, 0.12mg/g, 0.17mg/g, iron injection group was 3.16mg/g, 0.86mg/g, 0.07mg/g, 0.09mg/g and fat-free diet group was 7.41mg/g, 1.50mg/g, 0.11mg/g and 0.16mg/g, respectively. 3. In the mean contents of malondialdehyde in liver, kidney, muscle, testis and serum among groups, control group was 11.29nM/0.1g, 23.25nM/0.1g, 42.47nM/0.1g, 7.01nM/0.1g, 4.33nM/ml, iron injection group was 34.98nM/0.1g, 40.55nM/0.1g, 72.21nM/0.1g, 12.26nM/0.1g, 11.27nM/ml and fat-free diet group was 8.07nM/0.1g, 20.63nM/0.1g, 39.92nM/0.1g, 6.95nM/0.1g and 4.27nM/ml, respectively.

Ethanol 투여가 토끼의 혈중 포도당 및 Epinephrine 농도에 미치는 영향

허린수,Huh Rhin-Sou 대한수의사회 1979 대한수의사회지 Vol.15 No.8

Blood glucose and epinephrine concentrations of 15=hour fasted rabbits of 15 heads were determined to observe the efect of etthanol on the concentrations of the substances. Among the 15 rabbits 9 heads were treated with 5ml of $20\%$(vols.) eth

탈지사료 및 철분투여가 Rat에 있어서 지질과산화에 미치는 영향

허린수,김영흥,도재철,최연식 한국노화학회 1991 한국노화학회지 Vol.1 No.1