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      • 증례 : 제4뇌실의 수막종 - 증례보고 -

        한정인 ( Jung In Han ),김창현 ( Chang Hyun Kim ),문재곤 ( Jae Gon Moon ),조탁근 ( Tack Geun Cho ) 대한뇌종양학회 2012 대한뇌종양학회지 Vol.11 No.2

        Less than 50 cases of primary fourth ventricular meningioma are reported in literature. Most of them are hypo- to iso-signal intensity on T1-weighted images and iso- to mildly hyper-signal intensity on T2-weighted images compared with gray matter. Contrast enhancement is strong in all tumors, and the enhancement is homogeneous in 69% and heterogeneous in 31%. It could be harder to differentiate meningiomas of the fourth ventricle from ependymomas and choroid plexus papilloma. Most meningiomas in the fourth ventricle adhere to the choroid plexus. We present a new case and summarize features of magnetic resonance images.

      • KCI등재

        다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증

        천정환(Jung-Whan Chon),현지연(Ji-Yeon Hyeon),황인균(In-Gyun Hwang),곽효선(Hyo-Sun Kwak),한정아(Jeong-A Han),김무상(Moo-Sang Kim),김종현(Jong-Hyun Kim),송광영(Kwang-Young Song),서건호(Kun-Ho Seo) 韓國食品科學會 2011 한국식품과학회지 Vol.43 No.1

        본 연구에서는 두 종류의 선택배지를 활용한 배지배양법과 realtime PCR의 C. jejuni 검출능력을 비교하였다. 소시지, 쇠고기 분쇄육, 무순에 C. jejuni를 접종하고 Hunt broth로 증균배양 하였으며, mCCD agar와 Preston agar에 배양액을 획선도말하여 미호기적으로 배양하였다. 동시에 증균배양액에서 1 ㎖을 채취하여 realtime PCR을 실시하였다. 실험결과, real-time PCR은 쇠고기 분쇄육과 소세지에서 두 가지 선택배지와 비교하여 동일한 검출력을 보였으나 무순에서는 훨씬 더 많은 양성을 검출하였다(p<0.05). 두 배지간의 비교에서는 Preston agar와 mCCD agar는 통계학적 유의차가 없는 민감도를 보였다(p>0.05). 결론적으로 real-time PCR 은 표준검출법인 배지배양법과 비교하여 동등하거나 우수한 민감도를 지닌 신속검출기법인 것으로 사료되며, 배지배양법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 있어서 매우 유효한 방법이 될 것으로 판단된다. In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 ㎖) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.

      • KCI등재

        산모의 다중예측 인자를 이용한 임신 합병증 예후에 관한 다변량분석

        한인수(In Soo Han),한정열(Jung Yeol Han),고명인(Myong In Ko),최용관(Yong Kwan Choi),이홍복(Hong Bok Lee),양재혁(Jea Hyuk Yang),류현미(Hyun Mi Ryu),김문영(Moon Young Kim),김은성(Eun Sung Kim),한호원(Ho Won Han) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

        산모의 다중예측 인자를 이용한 임신 합병증 예후에 관한 다변량분석 목적: Maternal serum markers(AFP, uE3, hCG ), Unexplained down syndrome screen positive에 의한 태아예후를 비교하기위함. 연구방법: 1994년 3월부터 1996년 2월까지 임신 2기에 삼성제일병원을 방문하여 Triple marker test를 시행하고 본원에서 분만 기록이 있는 단태아 5,284례를 대 상으로 하였다. 각 산모 혈청 표지물질들은 radioimmunoassay kit를 이용하여 측정하였고 다운 증후군 스크린 양성은 α-software(Ver. 4.0)를 이용하여 산출하였다. 임신과 관련된 임신 합병증 예후의 기준은 조기태반 박리는 분만시 태반 소견을 기준으로 하였으며, 저체중아는 2500gm 미만, 만삭전 파수는 임신 37주 이전에 조기 양막 파수되는 경우로 하였다. 결과: 검사를 받은 산모의 평균 연령은 30±4.8세이며, 임신횟수는 평균 2.27, 출산횟수는 0.46이며, 검사를 시행한 평균 임신주수는 17.1주이었다. 저체중아는 357례(6.8%), 미숙아는 253례(4.8%), 전치태반은 108례(2.0%), 만삭전 파수는 68례(1.3%), 임신중독증은 66례(1.3%), 조기태반박리 24례(0.5%),자궁내 태아사망은 20례(0.4%)이었다. 이러한 임신 합병증 예후와 산모의 연령 AFP,hCG,uE3,그리고 Unexplained Down syndrome screen positive의 통계적 연관성에 관한 분석에서 35세 이상군은 조기태반 박리, 미숙아, 전치태반에서, AFP 2.0 이상군은 자궁내 태아사망, 저체중아, 임신중독증, 미성숙 조기 양수 파막, 미숙아에서, hCG 3.0 이상군은 자궁내 태아사망, 저체중아, 임신중독증, 미숙아, 전치태반에서, uE3 0.75 미만군에서는 자궁내 태아사망, 저체중아, 미숙아에서만, 그리고 unexplained Down syndrome screen positive에서는 조기태반 박리 , 자궁내 태아사망, 저체중아, 미숙아에서 통계적인 의미가 있었다. 또한 임신 합병증 예후와 관련된 혼란 변수를 조절하고 가장 영향력 있는 인자를 알기위해 multivariate logistic regression을 시행하였다. 자궁내 태아사망은 AFP 2.0 MoM이상, 저체중아는 AFP 2.0 MoM이상, hCG 3.0MoM이상, uE3 0.75 MoM 미만, 임신 중독증은 AFP 2.0 MoM이상, 미성숙 조기 양수 파막은 AFP 2.0 MoM이상, 미숙아는 AFP 2.0 MoM이상, 전치 태반은 35세 이상의 산모군과 hCG 3.0 MoM 이상군에서 P value 0.05 미만으로 통계적으로 의미있는 결과가 나왔으며, 조기태반 박리의 경우 35세 이상의 산모군에서만 P value 0.07이었다. 결론: 임신 합병증 예후를 예측하기위한 예측인자로서 산모의 고령 나이를 비롯해 산모혈청의 AFP, hCG, uE3과 Unexplained Down syndrome screen positive는 모두 다변량 분석에서 통계학적으로 의미가 있으며, 또한 다변량 분석에서는 증가된 AFP이 임신 합병증을 예측하는데 가장 신뢰성 있는 인자임을 알 수 있었다. Purpose : To evaluate a role as over 35 years, maternal serum markers, and a false positive screen for Down syndrome were the predictor of adverse pregnancy outcome. Materials and Methods : From Mar.1994, through Feb.1996, 5284 women were screened triple test to detect Down syndrome in the second trimester and were delivered Samsung Cheil hospital. The values of each maternal serum markers were measured with radioimmunoassay. And then, the screen positive of Down syndrome was calculated using α-software Version 4.0. The adverse outcome of the fetus and the mother included low birth weight(LBW) ( <2500gm), prematurity( <37 gestational weeks), placenta previa, preterm premature rupture of membranes(PPROM), pregnancy induced hypertension(PIH),abruptio placenta, and intrauterine fetal death(IUFD). The predictor markers included over 35years, elevated α-fetoprotein (AFP), elevated human chorionic gonadotropin(hCG), lowered unconjugated estriol (uE3), and a false positive screen for Down syndrome. Results : Mean age and mean gestational weeks in the study were 30±4.8 years and 17.1 weeks respectively. The adverse pregnancy outcomes were 357 LBW(6.8%), 253 prematurity(4.8%), 108 placenta previa(2.0%), 68 PPROM(1.3%), 66 PIH(1.3%), 24 abruptio placenta(0.5%), and 20 IUFD(0.4%). In univariate analysis, over 35 years was significantly associated with abruptio placenta, prematurity, and placenta previa, elevated MS-AFP( >2.0 MoM) associated with IUFD, LBW, PIH, prematurity, and PPROM , elevated MS-hCG (>3.0 MoM) associated with IUFD, LBW, PIH, prematurity, and placenta previa, lowered uE3 (<0.75) associated with IUFD, abruptio placenta, LBW, and prematurity.(P <0.05). In multivariate logistic regression analysis, IUFD was significantly associated with only elevated MS-AFP, LBW associated with elevated MS-AFP, elevated MS-hCG, and lowered uE3, PIH associated with only elevated MS-AFP, PPROM only elevated MS-AFP, prematurity only elevated MS-AFP, and placenta previa over 35 years, elevated MS-hCG.(P <0.05). However, abruptio placenta was not significantly associated with predictor markers.(P >0.05) Conclusions : Some predictors such as over 35 year, elevated hCG, lowered uE3, a false positive screen for Down syndrome were significantly associated with adverse pregnancy outcome. Also in multivariate analysis, we identified especially elevated AFP to be the most reliable predictor for adverse pregnancy outcome.

      • KCI등재

        해산식품과 채소에서 Vibrio parahaemolyticus 검출을 위한 배지배양법과 real-time PCR의 비교검증

        천정환(Jung-Whan Chon),현지연(Ji-Yeon Hyeon),황인균(In-Gyun Hwang),곽효선(Hyo-Sun Kwak),한정아(Jeong-A Han),정윤희(Yun-Hee Chung),송광영(Kwang-Young Song),서건호(Kun-Ho Seo) 韓國食品科學會 2010 한국식품과학회지 Vol.42 No.3

        V. parahaemolyticus는 국내에서 여름과 가을철에 걸쳐 발생되며 생선이나 패류 등의 해산식품을 날것으로 섭취하거나 불완전하게 익혀 먹을 경우 식중독을 일으키는 병원균이다. 본 연구의 목적은 표준 검출기법인 배지배양법을 이용한 V. parahaemolyticus의 검출과 real-time PCR을 이용한 V. parahaemolyticus의 검출에 있어서 그 유효성과 효율성을 검증하는 것이다. V. parahaemolyticus의 발생기록과 발생가능성이 있는 여러 해산식품과 무순에 적절한 균량을 접종하고 APW로 증균배양하였다. 증균배양이 끝난 후 TCBS선택배지에 배양액을 획선도말하고, 동시에 증균배양액에서 1 ㎖을 채취하여 real-time PCR을 실시하였다. TCBS에서 초록색이 나온 집락을 1-3개 선별하여 TSI 배지에 접종하여 screening test를 거친 후 API 20NE strip을 사용하여 확인동정하였다. 또한 정상세균총이 목적균의 성장과 검출에 어느 정도의 영향을 미치는지 평가하기 위하여 25 g의 식품 내 정상세균총의 수준을 함께 측정하였다. 실험결과, 자체 제작한 real-time PCR서열은 V. parahaemolyticus를 특이적으로 검출할 수 있었고 검출한계는 PBS에서 10³ CFU/㎖ 였다. 또한, 식품 내 정상세균총이 높을 경우 증균배양 시 목적균의 성장에 영향을 미칠 수 있다는 것을 확인하였다. Real-time PCR은 180개의 전체 샘플 중 76개의 양성 결과를 보여 66개의 양성 결과를 낸 배지배양법에 비해 더 많은 양성 검출율을 보였으나 통계학적인 유의차는 발견되지 않았다. Real-time PCR은 표준검출법인 배지배양법과 비교해 볼때 동등하거나 우수한 검출력을 지닌 것으로 보이며 이러한 realtime PCR법은 24시간 이내에 확정동정까지 가능하여 시간과 노동력의 소모가 많은 배지배양법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 매우 유효할 것으로 판단된다. Vibrio parahaemolyticus (V. parahaemolyticus), which is commonly found in raw seafood, causes gastroenteritis in humans. Rapid and effective methods have been developed as culture methods require up to 5-7 days. In this study, real-time PCR was compared with the standard culture method for detecting V. parahaemolyticus in seafood and radish sprout samples. Five hundred grams of the samples were artificially contaminated with V. parahaemolyticus then divided into 20 samples. The samples were incubated in alkaline peptone water and then streaked onto thiosulfate-citrate-bile saltssucrose agar. Biochemical tests for suspicious colonies were performed using the API 20NE strip. In parallel, real-time PCR was performed targeting the toxR gene using the enrichment broth. The real-time PCR was sensitive in discriminating V. parahaemolyticus from other foodborne pathogens. The detection limit of the real-time PCR was 10³ CFU/㎖ in phosphate-buffered saline. Although the real-time PCR detected more positive samples (76 out of 180, 42%) than the culture method (66 out of 180, 37%), there was no significant statistical difference (p>0.05) between the two methods. In conclusion, real-time PCR assays could be an alternative to the standard culture method for detecting V. parahaemolyticus in seafood and radish sprouts, which has many advantages in terms of detection time, labor, and sensitivity.

      • SCOPUSKCI등재

        접촉피부염 환자에서 최근 5년간 표준항원 첩포시험 성적

        배유인 ( You In Bae ),한정현 ( Jung Hyun Han ),윤숙정 ( Sook Jung Yun ),이지범 ( Jee Bum Lee ),김성진 ( Seong Jin Kim ),이승철 ( Seung Chul Lee ),원영호 ( Young Ho Won ) 대한피부과학회 2009 大韓皮膚科學會誌 Vol.47 No.11

        Background: A number of skin manifestations in patients with allergic contact dermatitis result from sensitization with specific allergens. The patch test is a primary diagnostic tool for identifying allergens causing allergic contact dermatitis. Objective: We identified the results of patch test with Korean standard series in patients with clinically diagnosed with contact dermatitis, and evaluated the specific relationship with each allergen. Methods: During a 5-year period, 743 patients were examined with patch test of standard Korean series. The results of patch test were then evaluated. Location of the skin lesion, age, sex, and occupation of the patients were completely reviewed. Results: Overall, 68.5% of patients had at least one positive reaction and 43.6% of patients had two or more positive reactions. Allergens most frequently causing positive reactions were nickel sulfate (27.1%), cobalt chloride (22.1%), potassium dichromate (17.9%), mercuric ammonium chloride (13.8%), and 4-phenyledediamine base (10.2%). Those patients in their 10s and 40s showed the highest rate of positive reaction. Metal allergens displayed higher positive rates than any other standard allergens. Of 201 patients who showed positive reaction to nickel, 37.8% and 27.3% of them also showed positive reaction to cobalt and chromium, respectively, representing concomitant reactions between metal-related antigens. In addition, polysensitization to cosmetics-related antigens including balsam peru and fragnance mix was evident. Conclusion: There was no significant difference in overall positive rate in patch test results compared with recent studies. However, we confirmed that metal-related allergens remain most common, and that multiple contact allergy were relatively common. (Korean J Dermatol 2009;47(11):1227~1235)

      • KCI등재

        소아 급성호흡곤란증후군에서 체외순환막장치 적용에 대한 RESP, PRESERVE, ECMOnet 점수 체계의 유용성

        안원기 ( Won Kee Ahn ),한정호 ( Jung Ho Han ),김윤희 ( Yoon Hee Kim ),설인숙 ( In Suk Sol ),윤서희 ( Seo Hee Yoon ),김민정 ( Min Jung Kim ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu-earn Kim ) 대한소아알레르기호흡기학회 2017 Allergy Asthma & Respiratory Disease Vol.5 No.3

        Purpose: With increasing use of extracorporeal membrane oxygenation (ECMO) for acute respiratory distress syndrome (ARDS) in children, development of standardized strategies for survival prediction has become crucial; however, this has not been accomplished yet. We evaluated the adult scoring systems for survival prediction used for their applicability in pediatric ARDS and validated them. Methods: A total of 11 children with ARDS receiving ECMO from 2013 to 2014 were evaluated with adult scoring systems, including the Respiratory Extracorporeal-membrane-oxygenation Survival Prediction (RESP), the PRedicting dEath for SEvere ARDS on VV-ECMO (PRESERVE), and the ECMOnet scores. We compared the scores on these scales and the clinical characteristics between survivors and nonsurvivors. Results: Eight of the 11 children died (72.7%). The PRESERVE score (survivors vs. nonsurvivors: 2 vs 5.25, P=0.048), and the ECMOnet score (4.1 vs. 5.63, P=0.048) were lower in survivors than in nonsurvivors. They correctly predicted mortality prediction. There was no significant difference in the RESP score between survivors and non-survivors (-4.33 vs -2.62, P=0.63). The parameters that showed significant differences in this study were peak inspiratory pressure, platelet, and delta neutrophil index. All children who were under immunocompromised conditions, such as those with tumors, or underwent hematopoietic stem cell transplantation died. The immunocompromised status should be considered an important factor for survival prediction in children with ARDS. Conclusion: This is the first pilot study to apply the survival prediction scoring system to pediatric ARDS with ECMO. It is necessary to establish and modify the survival prediction score system for pediatric ARDS with ECMO. (Allergy Asthma Respir Dis 2017:5:141-146)

      • 고속철도 무선통신 검측모듈 개발에 관한 연구

        김대연(Kim dae yeon),이재홍(Lee jae hong),한정석(Han jung seok),김태환(Kim tae hwan),이병곤(Lee Byeong gon),이헌수(Lee heon soo),유인식(You In sik),김덕수(Kim Duk Su) 한국통신학회 2014 한국통신학회 학술대회논문집 Vol.2014 No.1

        국내 고속철도 무선통신 시스템은 기술표준에 따라 APCO-P25(북미)와 TETRA(유럽) 방식이 혼용되어 운영중에 있다. 무선통신 검측시스템을 국내 환경에 맞도록 설계, 개발하여 안정적인 열차운행과 유지보수 등에 효율성을 제공하기 위한 시스템으로 외부요인에 의한 간섭 및 장비의 특성저하, 노후화 등 불량 요인으로부터 철도 시설물 규정에 따라 항상 최적의 상태로 유지 관리해야 할 필요성이 있다. 철도 시설물에 대한 전파환경 관리도 가능하여야 한다. 고속철도 검측시스템은 고속철도 열차무선 환경에서 서비스 중인 통신방식에 대해 전파환경을 측정하여 시설물의 이상 유무 및 양질의 서비스 제공을 위한 전파환경에 대한 기준을 제공한다. 또한 철도 시설물별 전파환경에 따른 핸드오버를 검측시스템에 적용하여 사용 중인 전파환경의 변화를 실시간 검측하여 효율적인 유지보수를 지원한다. 본 논문은 무선통신 검측시스템을 활용하여 전계강도 및 주파수, 핸드오버 및 불요파, 데이터 에러율을 측정하며, 분석 및 CB(데이터베이스)화를 위한 GUI와 통합서버 구축으로 국내 환경에 적합한 검측 방법을 제시하고자 한다. For system of the high-speed train radio system in Korea, the APCO-P25 (North American Standard) and TETRA (European Standard) systems are combined in operation. Design and development of measurement system for train radio equipment should take domestic environment into account and provide efficiency and consistency for stable operation and maintenance of trains. For this, such measurement system should be always kept in optimum conditions in line with the railroad facilities regulations and free of degradation by external factors, aging or fault. Methods to keep railroad facilities in optimum conditions include construction of database, comparison and analyze of changes of radio communication environmental between the past and the present, and management of radio environment for railroad facilities. Measurement of radio environment for communication method in service in high-speed train radio environment could provide criteria for distinction of deterioration of facilities and provision of quality service. By applying handover to measurement system, changes of radio environment in service could be measured in real-time for effective maintenance. The purpose of this study is to measure field intensity, frequency, handover, spurious and data error rate by using masurement system for radio communication and suggest inspection method appropriate for internal environment by building GUI for analysis and database and intergrated server.

      • KCI등재

        고구려 고분벽화의 사신도를 응용한 의상디자인 연구 - 발염을 중심으로

        한정인(Han Jung-In),유금화(Yu Kum-Wha) 한복문화학회 2009 韓服文化 Vol.12 No.3

        In this study, I choose Goguryeo’s ancient tomb mural as a competitive material, introduce its potentialities. It need identified cultural heritage to make globalization of traditional culture. "Sa-sin" mural has been estimated most beautiful painting in Goguryeo’smurals, because it use various printing techniques on "Denim" material. I reinterpret this "Sa-sin" mural to make historical and modern art work. I painted "Sa-sin" mural (that is a motive of my work) on modern dress, using "Denim" materials and dyeing techniques. I studied many processing techniques to reconstruct "Sa-sin" mural. Based on basic background, I reconstructed Sa-sin’s pattern of Goguryeo’s mural, introduce it in modern dress. I used feature of "Denim" material to make shape of Sa-sin. That feature is discharge printing which has similar effect of hand washing. Using "Denim" and "chiffon" materials I made 8 dresses that referred Goguryeo’s mural. Using discharge printing to Denim which is main material, I printed "Sa-sin-do" on dress. "Sa-sin-do" is a mural on "Kangsudaemyo" and "Kangsujoongmyo", consist of blue dragon, white tiger, "Joo-Jak", "Hyun-moo".

      • KCI등재

        연구논문 : Aspergillus oryzae로 발효한 자색당근을 첨가한 발효유의 품질 및 관능 특성에 관한 연구

        신배근 ( Bae Keun Shin ),강선아 ( Suna Kang ),한정인 ( Jung In Han ),박선민 ( Sunmin Park ) 한국식생활문화학회 2015 韓國食生活文化學會誌 Vol.30 No.3

        In this study, we compared the organoleptic and other qualities of fermented milk containing 10 or 15% purple carrot extract that had either been previously fermented with Aspergillus oryzae or not fermented. Fermentation characteristics, pH, chromaticity, viscosity, viable cell counts, and sensory evaluations were measured. The pH and acid values did not differ between purple carrot extract fermented with Aspergillus oryzae and non-fermented extract. Viable cell counts were significantly higher in 15% purple carrot extract fermented with Aspergillus oryzae compared to the control after fermentation. Regarding characteristic changes, purple carrot extract fermented with Aspergillus oryzae group showed a lower red value but higher yellow value compared with non-fermented purple carrot extract due to heat-sterilization. Both fermented and non-fermented extract groups showed significantly increased viscosity compared to control. In the sensory evaluation, 15% purple carrot extract fermented with Aspergillus oryzae showed the highest score. In conclusion, addition of 15% purple carrot extract fermented with Aspergillus oryzae resulted in a superior fermented milk product.

      • KCI등재

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