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인공수정 및 수정란이식 후 젖소의 혈액과 유즙에서 Progesterone과 Estrogen 농도 변화와 수태율과의 상관관계
한영훈,김홍래,조운비,김영훈,우제석,진동일,Han, Rong-Xun,Kim, Hong-Rye,Diao, Yun-Fei,Kim, Young-Hoon,Woo, Je-Seok,Jin, Dong-Il 충남대학교 농업과학연구소 2010 Korean Journal of Agricultural Science Vol.37 No.3
Early pregnancy diagnosis of bovine is an essential component for efficient reproductive plan in farms because long term of non-pregnancy results in economic losses by failure of offspring production and low milk yield in dairy cattle. The major steroid hormones related with reproduction are known to be progesterone and estrogen in bovine pregnancy. To evaluate detection level of hormones in milk, plasma and milk progestrone and estrogen of Holstein cows was analyzed during artificial insemination (AI) and embryo transfer (ET). Progesterone concentration at 21 days postestrus was significantly different in plasma and milk between pregnant and non-pregnant cows. Estrogen concentration at estrus was higher in pregnant recipients than that in non-pregnant recipients. To analyze correlation between hormone levels and conception rates in Holstein, the conception and return rates were checked following AI, and the returned cows were on the track of pregnancy after consecutive AI. Pregnant cows following first AI were considered as high conception group while pregnant cows following third AI were rated as low conception group. Proportion of high and low conception groups in this study was 78.2% and 9.1%, respectively. Hormone analysis indicated that high conception group had higher estrogen level during estrus than low conception group ($26.45{\pm}3.32$ vs $19.017{\pm}2.97$). Progesterone level was not different between high and low conception groups during estrus but increased significantly after 21 days postestrus (21 day: $4.95{\pm}1.12$ vs $0.95{\pm}0.23$, 35 day: $12.47{\pm}3.82$ vs $2.41{\pm}1.21$). In conclusion, the pattern of progesterone and estrogen secretion in Holstein milk samples could be a good candidate for early pregnancy detection and selection of recipients during ET.
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한영훈,김홍래,조운비,진동일,Han, Rong-Xun,Kim, Hong-Rye,Diao, Yun-Fei,Jin, Dong-Il 충남대학교 농업과학연구소 2011 Korean Journal of Agricultural Science Vol.38 No.2
The objective of this study was to develop a rapid and reliable PCR method for sexing of morula or blastocyst stage bovine embryo. BOV97M and bovine 1.715 satellite DNA sequences were selected for amplification of male and bovine specific DNA, respectively. But the unbalanced number of copies of these two repetitive sequences required some modification of PCR method. Karyotyping of blastomeres were carried for the confirmation of sex determination in bovine embryos. The coincidence rate of sex between biopsied-single blastomere and matched blastocyst was 80.0%. When in vivo- and in vitro- derived embryos were compared, 61.8% and 56.7% were male in in vitro- and in vivo-derived embryos, respectively. In vivo-derived embryos showed better hatching rate than in vitro-derived embryos following biopsy of blastomeres. In conclusion, rapid and effective PCR could be applied to sexing of bovine preimplantation embryos using single blastomere. The sensitivity of this assay may eliminate the need for biopsy of more than one nucleated blastomere and reduce trauma to the embryos derived from biopsy procedure.
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A Development of GPS SIS Anomalies Generation Software
한영훈,고재영,신미영,조득재 사단법인 항법시스템학회 2013 Journal of Positioning, Navigation, and Timing Vol.2 No.1
In this paper, GPS signal anomaly generation software is proposed which can be used for the analysis of GPS signal anomaly effect and the design, verification, and operation test of anomalous signal monitoring technique. For the implementation of anomalous signal generation technique, anomalous signals are generated using a commercial signal generation simulator, and their effects and characteristics are analyzed. An error model equation is proposed from the result of analysis, and the anomalous signal generation software is constructed based on this equation. The proposed anomalous signal generation software has high scalability so that users can easily utilize and apply, and is economical as the additional cost for purchasing equipment is not necessary. Also, it is capable of anomalous signal generation based on real-time signal by comparing with the commercial signal generation simulator.
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한영훈,김용희,허훈,곽윤근,Han, Y.H.,Kim, Y.H.,Huh, H.,Kwak, Y.K. 대한기계학회 1989 대한기계학회논문집 Vol.13 No.5
본 논문에서는 타이어의 각 부분의 물성치 계산을 위한 식을 유한요소법에 적용할 수 있도록 제안하였다. 이 식은 강철 코드의 굽힙효과를 고려 하였으며, 특히 각 요소에서 전단변형이 일어나는 동안의 굽힘효과를 고려하였다. 유한요소 공식화는 가상일의 원리에 의하여 평형 방정식으로부터 유도하였고, Updated refer- ence coordinate에 대해 증분해석을 적용하여 Updated Lagrangian공식화를 하였다. 그리고 차량하중에 의하여 타이어가 노면에 접지될때의 응력상태를 게산할 수 있도록 접촉문제 공식화를 유한요소 공식화에 첨가 하였다. The tire inflation and contact problem has been solved by a finite element method. The finite element formulation is derived from the equilibrium equations by the principle of virtual work in the form of an updated Lagrangian formulation for incremental analysis. Then, a contact formulation is added to the finite element formulation to calculate stress state of tire in contact with flat rigid road under the load due to the self-weight of a vehicle. In the finite element analysis, equations of effective material properties are introduced to analyze a plane strain model of the shell-like tire by considering the bending effect of reinforced steel cords. The proposed equations of effective material properties produced stress concentration around the edge of belt layers, which does not appear when other well-known equations of material properties are adopted. The result from the above algorithm demonstrates the validity of the formulation and the proposed equations for the effective elastic constants. The result fully interprets the cause of separation between belt layers by showing the stress concentration.
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