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한성옥,박은철,강대룡,강임옥,Han, Seong-Ok,Park, Eun-Cheol,Kang, Dae-Ryong,Kang, Im-Ok 대한예방의학회 2008 예방의학회지 Vol.41 No.5
Objectives : The purpose of this study was to measure the workload of home visit care activities and their relative values. This study examined also factors that affect the workload of home visit care activities. Methods : The participants of this study were 126 home-helpers of 50 home visit care agencies at the 2nd Long-term Care Insurance Demonstration Project. The workload of home visit care activities was divided into total work and four dimensions ; physical efforts, mental efforts, stress and time. Home visit care activities consisted of four categories with 24 items. We used magnitude estimation method to measure their relative values of the four dimensions. The participants answered the relative values of each activities based on the reference service. We used the activity for supporting their elderly's evacuation as the reference service. Results : Most of the respondents were over 40 years old female. They consumed most their time supporting elderly's going out. They consumed their highest physical, mental efforts, and stress for activities of coping with emergency situation. The Pearson correlation coefficients showed significant relationships between workload and each dimensions. This study showed that all four dimensions are statistically significant predictors of workload of home visit care activities. Also, we found that the home-helper's career affects the workload of home visit care activities. Conclusions : The workload of home visit care activities could be explained by physical efforts, mental efforts, stress and time.
C1 biorefinery by designer microbes
한성옥 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
The designer microbes are a key biological technology that can be used for biorefinery. The use of complexed enzyme systems is one of the strategies for effective bioresources utilization and offers considerably greater potential through a highly ordered structural organization that enables enzyme proximity synergy. Carbon monoxide (CO) was successfully efficiently converted by functional complexes containing carbon monoxide dehydrogenase and carbon monoxide sensing heme protein with enhanced CO binding affinity. Carbon monoxide sensing heme protein acts as mediator for increasing of carbon monoxide dehydrogenase activity by substrate proximity and assembled complexes caused an efficiently conversion of CO gas with aid of Carbon monoxide sensing heme protein. An enzyme complex for biological convertsion of CO to CO2 was anchored on the cell surface of CO2-utilizing Ralstonia eutropha and successfully showed 3.3-fold increased conversion efficiency. These results suggest that the complexed system may be a promising strategy for C1 biorefinery as biological tools.