Cryptosporidium parvum 단독 및 돼지 rotavirus와 혼합 감염시킨 자돈 장염의 병원성

한동운,강문일,박남용,위성환,Han, Dong-un,Kang, Mun-il,Park, Nam-yong,Wee, Sung-hwan 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1

The purpose of the present study was to understand the pathogenesis of infections in piglets inoculated with C parvum isolated from mice alone and combined with porcine rotavirus (S-80). Thirteen 10-day piglets were divided in four groups; Three, A group, were only given by C parvum. Four, B group, were orally administrated with firstly porcine rotavirus and then C patvum. Three, C group, were orally inoculated with porcine rotavirus alone. The rest, D group, were used as controls. During the experiment, there were daily recorded clinical signs including diarrhea to each pig. According to the periodic intervals for necropsy, all pigs were sacrificed from 4 to 12 days after the final inoculation of C parvum. Location and distribution of two pathogens, C parvum and rotavirus, in the intestinal mucosa of piglets tested were examined by pathological and immunohistological means. In addition, parasitological test using the feces of piglets was applied for the detection of cryptosporidial oocysts as well. A group showed diarrhea from 4 to 6 days post-inoculation(PI) and also discharged C parvum oocysts in feces during the day 4 to 7 PI. In tissue sections of jejunum and ileum, cryptosporidial oocysts were observed a few on the top of villi with slightly fusion. B group represented sign of diarrhea and discharge of oocysts from 2 to 11 days PI. There were some cryptosporidial oocysts both in the jejunal lumen and in the lumen of mucosal glands. As progressed, oocysts were most commonly distributed on the tip of villi of jejunum. Histopathologically there were also mild to moderately fused, attenuated focal desquamated, congested villi and mononuclear cell infiltration of varying degrees in the lamina propria of small intestine and colon at the day 4 and 7 PI. C group showed slightly to mildly attenuated and fused top of villi and mildly mucosal congestion. D group as controls was grossly and histopathologically normal in all parts of intestine. The present results indicate that the piglets inoculated with C parvum only are certainly milder in pathogenesis including duration of clinical course and severity of lesion than those in piglets concurrently infected with porcine rotavirus and C parvum. Also the strain (VRI-CN91) of C parvum used in the study has very low pathogenicity to occur enteritis of piglets.

한전의 중소기업 기술지원 사업

한동운,Han, Dong-Un 한국전기산업진흥회 1995 電氣産業 Vol.6 No.4

국내 서식 야생 고양이의 톡소플라즈마증, 한타바이러스 감염증 및 리켓치아성 질병에 대한 혈청학적 조사

한동운(Dong Un Han),이정길(Chung Gil Lee),강문일(Mun Il Kang),장환(Hwan Jang),김희선(Hee Sun Kim),김홍집(Hong Jib Kim),위성환(Sung Hwan wee) 한국예방수의학회 1999 예방수의학회지 Vol.23 No.4

1995년 11월부터 1997년 10월까지 2년간 전국을 5개 구역으로 구분하여 야외에서 서식중인 고양이 212두를 포획하여 톡소플라스마와 한타바이러스 및 리켓치아에 대한 항체보유율을 조사하였다. 톡소플라스마 항체 검출을 위하여 포획된 고양이의 혈청에 대하여 Latex 응집반응을 실시한 결과 79.3% (168/212)가 음성소견인 32배 미만의 항체가를 나타냈고 20.7% (44/212)에서 32배 이상의 항체가를 나타냈다. 항체 양성소견을 보인 44두에 대한 항체 역가별 분포도는 1:32~256 배가 38.6%, 1:512~2,048 배가 31.8% 그리고 4,096 배 이상이 29.5%로 역가별로는 유의성 있는 차이를 보이지 않았다(P<0.05). 국내의 야외 고양이의 톡소플라스마에 대한 암수간의 항체 보유율의 차이는 수컷이 22.3%(23/103)로 암컷의 19.3%(21/109)에 비해 다소 높게 나타났다. 고양이의 지역별 항체보유율은 서울ㆍ경기(31.9%)가 가장 높았고, 다음으로 강원(24.4%), 전라(17.5%), 충청(15.0%)지역의 순으로 나타났으며, 경상(12.5%)지역이 가장 낮은 항체수준을 나타냈다. 연령증가에 따른 항체보유율은 4세 이상이 25%(3/12), 2~4세가 21.6%(11/51), 1~2세가 22.4%(19/87), 1세미만에서 19.4%(11/62)으로 연령별로는 유의성있는 차이가 관찰되지 않았다. 검사대상 야외고양이 중 지역별로 165두에 대하여 한타바이러스와 scrub typhus, murine typhus와 spotted fever group rickettsia 등 3종의 리켓치아에 대한 간접형광항체법으로 검사한 결과 다른 질병의 감염 여부와 관계없이 혈청내에서 항체 양성소견이 관찰되지 않아 현재까지 국내의 고양이들은 이들 질병에 감염이 이루어지지 않은 것으로 판단되었다. This study was performed to obtain the recent information of sero-prevalence against zoonotic diseases including toxoplasmosis, hantanvirus infection, scrub tyhpus, murine typhus, and spotted fever group, transmitted by cats. Serum, samples of cats were collected from five regions in Korea from November 1995 to Octorber 1997. Basic anatomic and pathophysiologic conditions of all cats collected were recorded. Sera were screened by indirect immunoflurescent antibody assay for the presence of specific antibodies against Rickettsia tsutsugamushi, R. typhi and R. sibirica, and hantavirus. For Toxoplasma gondii, there was used by latex aggultination test. Seropositives against T. gondii in 212 stray cats from SeoulㆍKyounggi (47), Kangwon (45), Choungchong (40), Cholla (40), and Kyoungsang (40) were 20.7% (44 cases). Seropositive antibody titers tested were classified as 38.6% in 1:32~256, 31.8% in 1:512~2,048, and 29.5% in 1:4,096~32,768. By sex, male(22.3%) showed higher in seropositives than female (19.3%). No seropositives to hantavirus, Rickettsia tsutsugamushi, R. typhi and R. sibirica in 165 stray cats were detected. Stray cats in Korea were fortunately not invaded by zoonotic causative agents including hantavirus and main zootic rickettsial pathogens.

랫드에서 옻나무 추출액(Rhus-II)의 4주 반복 투여 안정성 평가

최창순,한동운,Choi Changsun,Han Dong Un 한국식품위생안전성학회 2005 한국식품위생안전성학회지 Vol.20 No.1

The purpose of this study was to examine the four week repeated toxicity in Sprague-Dawley rats orally administrated with Rhus-II (water fraction of Rhus Veniciflua). In acute toxicity test, three groups (40 rats of both sex) were administrated different dosages of Rhus-II, 2 g/kg (high dosage group), 1 g/kg, 0.5 g/kg and one group (10 rats of both sex) were received by orally only saline according to the Regulation on Korea Food and Drug Administration, respectively. There was no difference in body weight change, feed intake and water consumption among different dose groups. There was no alteration in relative organ weight by the administration of Rhus-II. No death of abnormal clinical signs was observed during the experimental period. Between the groups orally administered Rhus-II and the control group, there was no statistical significance in urinalysis, hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by four week treatment with Rhus-II. These results suggest that no observable effect level(NOEL) of the test orally administration was considered to be more than 2g/kg in rats under the conditions employed in this study.

Argyrophilic nucleolar organizer region(AgNOR) 염색을 이용한 림프구의 미세핵분석법의 개선

김성호,한동운,강문일,임정택,Kim, Sung-ho,Han, Dong-un,Kang, Mun-il,Lim, Jeong-taek 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2

A technique to improve the analysis of micronuclei(MN) in lymphocytes as a cytogenetic indicator is reported. For the purpose of diminishing the variation of the result from individual reader and making it easier to distinguish accurately a cytokinesis blicked(CB) lymphocyte and micronuclei, we tried a modified one-step silver staining technique as a method for detection of the argyrophilic nucleolar organizer region(AgNOR) with or without conventional Giemsa stain in the slide from CB method. Compared with the conventional Giemsa stain, the preparation processed with this method are especially useful for the accurate analysis of MN of cultured lymphocyte with cytochalasin B. This method will be a useful technique for automated calculation of MN.

국내 사육 돈군내 Porcine cytomegalovirus에 관한 혈청역학적 연구

강문일,한미,한동운,김희선,김병한,김홍집,안수환,Kang, Mun-il,Han, Mi,Tajima, Tomoko,Han, Dong-un,Kim, Hee-sun,Kim, Byung-han,Kim, Hong-jib,Ahn, Soo-hwan 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

The purpose of this study was to investigate the confirmation and prevalence of porcine cytomegalovirus (PCMV) infection of pigs in Korea using enzyme-linked immunosorbent assay (ELISA). Four hundred-eighty one sera tested were collected from the areas of Kyonggi, Kangwon, Chungcheong, Cholla, Gyongsang and Cheju during the year of 1991 to 1997 except 1994. PCMV antigen, OF-1 strain, for ELISA, was prepared 19-PFT-F cell line originated from porcine fallopian tube. Positive control was used by sera made from the specific pathogen free piglets which were infected with OF-1 strain. Three hundred-sixty seven of 481 sera (76.3%) were positive against PCMV. Antibody titers of these seropositives were classified by 129 (26.8%) cases in more than 1 : 12,800, 77 (16.0%) in 1 : 6,400, 76 (15.8%) in 1 : 3,200, 44 (9.2%) in 1 : 1,600, and 41 (8.5%) in 1 : 800, respectively. Also, the seropositive pigs were divided by 87.4% (76/87) in older than 6 month-old, 73.9% (238/322) in 2~6-month old, and 73.6% (53/72) in less than 2-month old, respectively. Regional prevalence rate of PCMV infection in Korea showed 89.7% (70/78) in Chungchong, 79.8/% (83/104) in Cholla, 79.4% (143/180) in Kyonggi, 79.3% (42/53) in Gyongsang, 50% (15/30) in Kangwon, and 38.9% (14/36) in Cheju area, respectively. In the sera collected in 1991, seropositive rate was appeared as 90.2% (37/41). From 1992 to 1997 except 1994, the average infection rate to PCMV was 77.5%. Consequently, these results confirmed that PCMV in Korean piggeries was introduced at least before the year of 1991. More importantly, PCMV infection has been prevailing nation-wide in pig herds in Korea.

가시오가피와 더덕 추출물을 첨가한 발효유가 마우스의 면역기능에 미치는 영향

임상동(Sang-Dong Lim),성기승(Ki-Seung Seong),김기성(Kee-Sung Kim),한동운(Dong-Un Han) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.3

가시오가피의 변역활성 증진 효과를 알아보기 위하여 선발된 수컷 마우스를 대상으로 하여 발효유에 가시오가피와 더덕 열수 추출건조물(가시오가피 : 더덕 = 8:2)을 혼합하여 투여한 그룹 1㎎/㎖(A), 3 ㎎/㎖(B), 9 ㎎/㎖(C)의 3그룹으로 나누어 임상적용 경로인 경구투여를 선택하여 발효유를 각각 3 ㎖/㎏씩 위내로 직접 투여하였다. 대조군은 발효유만 먹인 그룹(D)과 식염수만 먹인 그룹(E)을 두었다. 오가피의 함량이 ICR계 수컷 마우스의 면역기능에 미치는 영향에 대해 알아본 결과 모든 군에서 마우스의 증체량과 체중 증가는 유의성 있는 차이를 보이지 않았고, 7주와 10주령에 안락사 시킨 마우스의 각 장기의 무게에서도 유의성 있는 차이를 보이지 않았다. 비장계수는 7주령에서는 B군이, 10주령에는 C군이 유의성 있는 증가가 관찰되었다(p<0.05). 항체생산세포수는 식이급여 7주에 B와 C군이, 10주에 C군이 각각 대조군에 비해 통계적인 유의성이 있었다(p<0.05). 양적혈구에 대한 응집소가는 식이의 급여기간이 길어질수록 감소하였는데, 7주차에는 A, B, C군에서, 10주차에서는 B와 C군에서 유의성이 있었다(p<0.05). 혈중 면역글로불린(IgG)의 수치는 7주에서는 C군이, 10주에서는 B와 C군이 유의성이 있었다. 림프구는 B와 C군이 대조군보다 증가하였고, 비장 및 흉선조직에서도 B와 C군이 활발한 면역반응을 보였다. 이상의 결과 가시오가피와 더덕 열수추출건조물이 생체 내에서 면역력을 증강시킬 수 있을 것으로 사료되었다. We investigated the immunomodulatory actions of water extract from Acanthopanax senticosus in male ICR mice. The mice were treated with fermented milk containing three added doses of freeze dried extract: 3 ㎎/㎏ (A), 9 ㎎/㎏ (B), and 27 ㎎/㎏ (C) of body weight with Acanthopanax senticosus: Codonopsis lanceolata (8:2) for 7 and 10 weeks, respectively. Organ weights, plaque-forming cell tests, agglutination tests, IgG tests, differential white cell counts, and histological tests were performed at the 7th and 10th weeks of dietary treatment. There were no significant differences in body weight and organ weight. The spleen indices of group B at 7 weeks and group C at 10 weeks were significantly higher than those of the control group (p < 0.05). For the plaque-forming cell test, groups B and C at 7 weeks, and group C at 10 weeks, showed significant increases over the control group (p < 0.05). The agglutination test decreased with an extended experimental period. Groups A, B, and C at 7 weeks, and groups B and C at 10 weeks, had greater antibody responses to sheep red blood cells (SRBC) than the control group. The IgG antibody production of group C at 7 weeks and groups B and C at 10 weeks were significantly higher than the control group (p < 0.05). In groups B and C, lymphocyte percentage was higher than the control group, and their spleen and thymus tissues showed active immune reactions.

흰털오가피와 더덕 추출물을 첨가한 발효유 급여가 마우스의 면역기능에 미치는 영향

임상동,성기승,김기성,한동운,Lim, Sang-Dong,Seong, Ki-Seung,Kim, Kee-Sung,Han, Dong-Un 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.1

흰털오가피의 면역활성 증진 효과를 알아보기 위하여 선발된 수컷 마우스를 대상으로 하여 발효유에 흰털오가피 (뿌리 : 잎 : 줄기)와 더덕 열수추출건조물을 (5 : 2 : 1.5) : 1.5 비율로 혼합하여 투여한 그룹 1 mg/mL(A), 3 mg/mL(B), 9 mg/mL(C) 3그룹으로 나누어 임상적용 경로인 경구투여를 선택하여 발효유를 각각 3 mL/kg씩 위내로 직접 투여하였다. 대조군은 발효유만 먹인 그룹(D)과 식염수만 먹인 그룹(E)을 두었다. 흰털오가피의 함량이 ICR계 수컷 마우스의 면역기능에 미치는 영향에 대해 알아본 결과 모든 그룹에서 마우스의 증체량과 체중 증가는 유의성 있는 차이를 보이지 않았고, 7주와 10주령에 안락사 시킨 마우스의 각 장기 무게에서도 간, 신장, 심장, 폐, 고환의 무게에서는 유의성 있는 차이를 보이지 않았다. 비장계수는 7주령 C군과 10주령 B군에서 유의성(p<0.05)있는 증가가 있었으며, 식이 지속기간이 길어질수록 증가된 반면, 흉선계수는 모든 군에서 유의성 있는 증가가 관찰되지 않았다. 항체생산 세포수는 흰털오가피 투여군이 대조군에 비해 증가하였으며, 7주령에 비해 10주령이 감소하는 경향을 보였다. 항원에 대한 항체 생성량을 알아보기 위하여 혈구응집반응을 실시한 결과 흰털오가피 투여군이 대조군에 비하여 증가하는 경향을 보였고 10주령 C군에서 유의성 있는 차이를 보였다. 면역글로블린 농도는 흰털오가피 투여군에서 증가하였고, 7 및 10주령 C군에서 유의성이 있었다. We have investigated the immunomodulatory activity of water extracts of Acanthopanax divaricatus var. alveofructus in male ICR mice. Mice were administrated with fermented milk containing freeze-dried extract 3 mg/Kg (A), 9 mg/kg (B), 27 mg/Kg (C) per body weight with A. divaricatus var. alveofructus (loots : leaves : stem) : Codonopsis lanceolata = (5 : 2 : 1.5) : 1.5 for 7 and 10 weeks, respectively. Body weight, relative organ weight, cellularity of lymphoid organs, plaque- forming cell (PFC) assay, agglutination (AGG) test and lymphoproliferation were examined in various groups of animals. Any significant differences of body weight gain were not recorded in the tested ICR mice. There was significant different (p<0.05) in the spleen index in B group of 10 weeks and C group of 7 weeks fed mouse. The thymus gain weight was increased during administration of the extract, but there was no significant increase on other organs gain. Humoral immunity as measured by PFC showed more decreased PFC level in 10 weeks than in 7 weeks. In the HT, A. divaricatus var. albeofructus extract also showed a significant increase (p<0.05) in C group of 10 weeks. Administration of extracts from A. divaricatus var. albeofructus increased significantly in the production of IgG antibodies on the mice immunized with SRBC in B group of 7 and 10 weeks (p<0.05).

간접형광항체법(間接螢光抗體法)에 의한 개의 바이러스-canine distemper virus, canine parvovirus, canine coronavirus, canine adenovirus type-2, canine parainfluenzavirus-항체분포 조사

윤기복,강문일,박남용,한동운,Yoon, Ki-bok,Kang, Mun-il,Park, Nam-yong,Han, Dong-un 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1

An indirect immunofluorescent antibody test was applied to survey the antibody prevalence on five canine viruses including canine distempervirus(CDV), canine parvovirus(CPV), canine coronavirus(CCV), canine adenovirus type-2(CAV-2), canine parainfluenzavirus(CPIV) in dogs. The period studied was from October 1992 to June 1993. A total of 80 dog sera was collected from veterinary clinics in Kwangju and Seoul, and examined for the presence of virus antibodies. Immunofluorescent antibodies(IFA) to all viruses were present in a high percentage of 80 sera tested. Seventyfive(93.8%) showed detectable IFA against CPV, 67(83.8%) against CDV, 51(63.8%) against CCV, 42(52.5%) against CPIV and 34(42.5%) against CAV-2. These suggested that all viruses were endemic in the communities. IFA levels against each virus were also distributed fairly irregularly. IFAs for CDV and CPV were detected more frequently with a relatively high incidence in vaccinated group less than 1 years of age. IFAs for CAV-2 were detected more frequently with growing age. In the correlation of clinical signs and antibody prevalence, dogs that showed hematochezia and vomiting had high titers in the positive sera is noteworthy, particularly for CDV and CPV. The significance between dogs those who had diarrhea, dyspnea and salivation and those viruses were obscure.

한우 송아지의 질병발생과 폐사율 조사

강문일 ( Mun Il Kang ),한동운 ( Dong Un Han ),정용운 ( Yong Un Chung ),정도영 ( Do Young Chung ),이채용 ( Chai Yong Lee ),이정길 ( Chung Gil Lee ),위성환 ( Sung Hwan Wee ),조재진 ( Jae Jin Cho ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.3

From September 1996 to September 1999, 419 Korean-native calves with diseases under 6-month old collected from Kyonggi, Chungcheong, Chonlla and Kyongsang were examined by clinical, microbiological, parasitic, hematologic and histopathological mean. Among them, 124 cases were tested about the neutralization antibodies against infectious bovine rhinotracheitis virus(IBRV), parainfluenza-3 virus(PI-3V), bovine viral diarrhea virus(BVDV), bovine ephemeral fever virus(BEFV). In calf diseases in the survey, enteric diseases(72.8%) were most frequently involved and the following orders were taken by respiratory(17.4%) and reproductive(5.0%) disorders. In the causative pathogens associated with calf diseases and motality, 48.4% was induced by bacteria origin and also 35.6% by viral agents. Calf mortality was up to 76.3% in the cae of bacterial diseases and 55.7% in viral diseases. In bacterial diseases, frequent disorders were composed of colibacillosis(52.7%), salmonellosis(13.8%), pasteurellosis(12.8%) and campylobacteriosis(3.9%) and their mortalities showed 73.8% in colibacillosis, 73.0% in pasteurellosis, 67.9% in salmonellosis and 50.0% in campylobacteriosis(50.0%). Among the outbreaks of viral diseases, there were BVD(22.8%), bovine rotavirus infection (20.8%), bovine coronavirus infection(16.8%), bovine respiratory syncytial virus infection(15.4%), IBR(15.4%), Akabane disease(4.7%) and Chuzan diseases(3.4%). Interesting results through this studies were obtained the first isolate to Chuzan virus and Ainovirus in Korea which could be promised the development for diagnostic method and vaccines soon. Calf mortality to Akabane and Chuzan diseases was 100%. Main parasitic diseases were occupied by coccidiosis and babesiosis and their mortality of babesiosis was 20.0%. Other diseases were abomasal impaction(6.7%) and toxicosis(4.5%). The mortality of abomasal impaction was 89.3%. In some causes with malformations(1.9%) were confirmed as anasarca, derodidymus, polymelia, humerus hypoplasia, and tracheal collapse. Calf diseases had mostly been occurred in one month old group(52.5%) and its prevalence was 25.1% in two to three month old group and 22.4% in four to six month old group. In calf mortality by age, there were 37.9% in one month old group, 18.1% in two and three month old group, and 13.8% in four to six month old group, respectively. The older the age of calf, the less the prevalence of calf enteric diseases. Respiratory diseases in calves to be tested frequently occurred in one to two month old group (41.4%). In one month old calves, the prevalence of enteric disease was 80.0%(p<0.05) and that of reproductive and respiratory disease was 9.5% and 8.2%, respectively. In two month old and four to six month old, enteric disease was 65.7% and 63.8% and respiratory disease was 28.6% and 26.6%. Seasonal prevalence and mortality of Korean-native calf diseases were not a significant difference. Prevalence of calf diseases in summer(31.5%) frequently occurred to compare that in winter(20.3%). Abortion and malformation in calves frequently occurred in spring. Hematological values in 84 calves with clinical signs showed mild to marked leukocytosis. Also, there was slight increase in hematocrit, platelet, mean corpuscular volume and mean plasma volume, but all of those were included the higher level to normal ranges. Calves with respiratory signs showed slightly erythrocytosis. One hundred seventy three calves without clinical signs were not significant different to ill cases in hematological values, but number of platelets was in higher normal range. In 125 calves, 84.8% was taken the antibody to IBRV, but 72% with the antibody had recorded the titer level lower than log(2)5. The neutralizing antibody levels of higher than log(2)5 to PI-3V and BVD virus were 60.8% and 67.2% cases, respectively. There were the cases of 57.6% had the neutralizing antibody level lower than log(2)5 to BEFV.