Swim-up, Percoll, Sil-Select를 이용한 정자처리법에 의한 정자회수율, 운동성 및 체외수정율의 비교분석

하정희,엄기붕,정형민,정미경,김현규,고정재,윤태기,차광열,Ha, J.H.,Oum, K.B.,Chung, H.M.,Chung, M.K.,Kim, H.K.,Ko, J.J.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

It is well known that discard of seminal plasma from the semen and separation of motile sperm should be preceded before insemination for IUI or IVF. Till now, more than ten kinds of semen treatment methods have been developed. Of those, swim-up and Percoll methods have been used widely in ART laboratories as a routine semen treatment methods because of its advantages. However, there are reports that Percoll can make a genetic trouble because of its chemical structure and therefore the necessity has been arisen to substitute Percoll for other equivalent materials. This study was performed to evaluate the effects of three different sperm preparation methods (swim-up, Percoll and Sil-Select) on sperm motility, sperm recovery rate and fertilization rate. Also, the feasibility of using Sil-Select instead of Percoll in ART was evaluated. Each semen samples were divided into three fractions and motile sperm were recovered by swim-up, Percoll and Sil-Select gradient centrifugation methods. Normal and sub-normal criteria of fifteen semen samples and seventeen IVF cycles were included in these study. As results, no significant difference was found in sperm recovery rate in normal semen treated by a Swim-up, Percoll and Sil-Select method ($13.2{\times}10^6,\;17.5{\times}10^6\;and\;17.7{\times}10^6$ respectively). The initial sperm motility was 61.9% and this increased to 87.1%, 92.6% and 89.5% through Swim-up, Percoll and Sil-Select treatment, respectively. Higher motility was observed in Percoll and Sil-Select treated groups (81.5%, 79.2%, respectively) than swim-up group (66.8%) after incubation for 24hrs. In sub-normal group, sperm recovery rates were higher in Sil-Select group $(2.9{\times}10^6)$ than Percoll gradients group $(1.8{\times}10^6)$. In IVF cycles, the outcomes of fertilization using sperm treated by swim-up and Sil-Select group were similar (82.2%, 79.7% respectively). In conclusion, our results indicate that Sil-Select can be used as a substitute material for sperm preparation instead of Percoll.

생약의 Benzodiazepine 수용체 효능활성 검색 (II). -천마성분 및 유효분획의 활성-

하정희,용철순,김정애,허근,이동웅,Ha, Jeoung-Hee,Yong, Chul-Soon,Kim, Jung-Ae,Huh, Keun,Lee, Dong-Ung 한국생약학회 1999 생약학회지 Vol.30 No.3

In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of $[^3H]Ro15-1788$, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of $[^3H]flunitrazepam$ binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.

생약의 Benzodiazepine 수용체 효현활성 검색(I) -수종의 신경안정 생약 추출물에 대한 활성 검색-

하정희,박용기,강병수,이동웅,Ha, Jeoung-Hee,Park, Yong-Ki,Kang, Byung-Soo,Lee, Dong-Ung 한국생약학회 1999 생약학회지 Vol.30 No.2

This study was aimed to evaluate an agonistic activity to benzodiazepine receptor of several medicinal plants, which have been used as sedatives in oriental medicine. Methanol extracts of medicinal plants which were used in this study inhibited the binding of $[^3H]Ro15-1788$, a selective benzodiazepine receptor antagonist to benzodiazepine receptor of rat cortices. Inhibitory activity of Cyperus rotundus was observed to be the highest among the tested medicinal plants. Methanol extracts of Cyperus rotundus and Zizypus jujuba inhibited a $[^3H]flunitrazepam$, a selective benzodiazepine receptor agonist, binding to benzodiazepine receptor. GABA significantly enhanced the inhibition of $[3H]flunitrazepam$ binding by Cyperus rotundus and Zizypus jujuba, and these positive GABA shifts supported the strong possibility of agonistic activity to benzodiazepine receptor. From these results, it may be concluded that the substance or substances with neurochemical properties characteristic of a benzodiazepine receptor agonist may be important components and contribute to the sedative property of these medicinal plants.

예비유아교사의 다문화 감수성 함양을 위한 예술교육의 방향 탐색

하정희(Ha, Jeong-Hee) 한국어린이교육문화비평학회 2021 영유아교육과정연구 Vol.11 No.1

우리나라는 다문화 사회로 빠른 변화를 겪고 있으며 우리 사회 전반에서 다문화, 다인종을 만나는 것이 흔해졌을 뿐만 아니라 우리의 생각이나 삶의 방식에도 영향을 미치는 다문화 상황에 놓여있다. 유아교육 현장에서 교사는 다문화 가정 자녀에 대한 교육 실행의 어려움에 직면하고 있으며 유아교사양성기관은 예비유아교사를 대상으로 보다 교육 실천력이 높은 다문화교육을 실시할 필요성이 긴급해졌다. 이에 본 연구는 유아교사로서 함양해야 할 역량으로서 다문화 감수성을 선정하였으며 기존의 전공 교과목과의 통합을 통해 그 교육적 효과를 높이는 방안을 제시하였다. 다문화 감수성 함양을 위한 다문화 통합교육 프로그램 구성의 가능성을 예술교육에서 탐색하였다. 본 연구는 다문화 감수성 함양을 위한 예술교육의 방향으로 예술교육과 다문화 감수성 교육의 목표 간 적절한 균형 달성, 예술교육과 다문화 감수성 통합을 위한 교육내용의 선정 및 조직화, 예술적 상상력지향의 예술교육 방법 모색, 다중감각을 활용하는 교육 프로그램의 구성, 협력과 의사소통의 강화 등 이상 5개 방향을 제시하였다. 본 연구의 결과는 예비유아교사의 다문화 역량 함양을 위한 구체적인 교육 실행 방향을 제시함으로써 다문화 통합 교육 프로그램 구성을 위한 기초연구로서의 가치가 있다. Our country is undergoing a rapid change to a multicultural society. It has become common to meet multicultural and multi-ethnic people across our society, and it is in a multicultural situation that also affects our thoughts and ways of life. At the site of early childhood education, teachers are facing difficulties in implementing education for children from multicultural families, and early childhood teacher training institutions need to provide more practical multicultural education for preservice teachers. Therefore, this study selected multicultural sensitivity as a competence to foster as an early childhood teacher and suggested ways to enhance educational effectiveness through integration with existing major subjects. Art education explored the possibility of organizing a multicultural integrated education program to foster multicultural sensitivity. This study presented five directions: achieving an appropriate balance between artistic education and multicultural sensitivity education goals, selecting and organizing educational content for integrating artistic and multicultural sensitivity, creating multiple-sense education programs, and strengthening cooperation and communication.

COVID-19와 어린이·청소년 신체활동 감소에 대한 글로벌 시각

하정희 ( Ha Jung Hee ) 중앙대학교 학교체육연구소 2023 Asian Journal of Physical Education of Sport Scien Vol.11 No.4

이 연구는 COVID-19와 신체활동 감소 경향을 바탕으로 우리나라에서의 어린이·청소년 신체활동 가이드라인 보급과 활용, 어린이·청소년의 신체활동 수준과 글로벌 감시체계 현황, COVID-19 팬데믹 기간 중의 어린이·청소년 신체활동에 대한 현황 분석을 통하여 다음과 같은 결론을 제시한다. 첫째, 우리나라 국민건강증진 정책에서 어린이·청소년 신체활동 연관 지표가 우선적인 국가수준 관리지표로 다루어져야 한다. 둘째, 어린이·청소년 신체활동 좌식행동 글로벌 감시체계 네트워크 참여를 적극 지지하고 참여를 확대해야 한다. 셋째, 코로나와 같은 팬데믹은 향후에도 발생할 수 있는 만큼 신체활동을 통한 위험 감소에 노력하여야 하며, 특히 어린이·청소년들의 신체활동의 신체적 심리·정서적 건강 유익성에 대한 이해를 높여야 한다. The purpose of this study was to investigate the global trend of decreased physical activity level in children and adolescents caused by COVID-19, and provide informational data to facilitate policies for promoting physical activity among children and adolescents in South Korea post COVID-19. Based on the findings, the study recommends the following: Firstly, assessment indicators related to physical activity among children and adolescents should be given priority as national assessment indicators in national health promotion policy. Secondly, there should be strong support for active participation and expansion of the global surveillance network for sedentary behavior in physical activity among children and adolescents. Thirdly, given that pandemics such as COVID-19 may occur in the future, it is imperative to focus on risks through physical inactivity, and in particular, increasing awareness of health benefits for children and adolescents.

완벽주의와 역기능적 인지가 학습자의 자기결정성 및 학업성취에 미치는 영향

하정희(Ha Jung Hee),임연욱(Im Yeon Wook),하영자(Ha Young Ja) 학습자중심교과교육학회 2011 학습자중심교과교육연구 Vol.11 No.2

본 연구는 대학생 279명을 대상으로 하여 완벽주의와 역기능적 인지의 관련성을 살 펴보고, 완벽주의와 역기능적 인지가 자기결정성 및 학업성취에 미치는 경로를 살펴 보고, 이에 대한 구조모형을 검증하였다. 연구결과, 자기지향적 완벽주의는 역기능적 인지의 하위변인인 역기능적 불안 및 역기능적 공격성에만 부분적으로 부적 상관을 나타내는 반면에, 사회적으로 부과된 완벽주의는 역기능적 인지의 하위변인인 역기능 적 우울, 역기능적 불안, 역기능적 공격성 모두와 정적 상관을 나타내었다. 또한 경쟁 모형이 최종모형으로 선택되었는데, 이는 사회적으로 부과된 완벽주의 성향이 클수록 역기능적 인지 수준이 커지게 되고, 역기능적 인지 수준이 커질수록 자기결정성이 낮 아진다는 것이다. 한편, 학업성취는 사회적으로 부과된 완벽주의와 부적 상관을 나타 내었다. 이와 같은 결과를 통해 학습자의 자기결정성에 영향을 주는 변인들의 특성 및 과정을 살펴볼 수 있었으며, 마지막으로 이 연구에 대한 교육적 의의와 후속연구에 대 한 제언을 하였다. This study investigated 279 college students to explore the relationship between perfectionism and dysfunctional cognition and the way how perfectionism and dysfunctional cognition affect self decision making and academic achievement, and finally examined the structural model on them. The results show that self oriented perfectionism has negative correlation partly only with dysfunctional anxiety and dysfunctional aggression, which are the sub-variables of dysfunctional cognition. On the other hand, socially-prescribed perfectionism shows positive correlation with all of the dysfunctional depression, dysfunctional anxiety, dysfunctional aggression which are the sub-variables of dysfunctional cognition. In this study, the competitive model was chosen as the final model. This means that dysfunctional cognition increases as socially-prescribed perfectionism increases, and self decision making decreases as dysfunctional cognition increases. Academic achievement shows negative correlation with socially-prescribed perfectionism. With these results, this study analyzes the features of the variables which affect students self decision making. At last educational implications of this study is presented and the further studies are suggested.

연령집단구성에 따른 유아의 사회적 놀이 유형 비교

하정희(Jeong Hee Ha) 한국영유아교원교육학회 1998 유아교육학논집 Vol.2 No.1

역할놀이의 연령별 특성 탐색

하정희 ( Jeong Hee Ha ),성은영 ( Eun Young Sung ),이혜경 ( Hye Kyoung Lee ) 영유아교육학회 2005 영유아교육연구 Vol.8 No.-

한국체육대학교와 조선체육대학의 스포츠교류 제안

하정희(Ha, Jung-Hee),손환(Son, Hwan) 한국체육과학회 2016 한국체육과학회지 Vol.25 No.3

This Study had a research to examine a new direction of sport exchange between south and north Korea. For the research, this study on the background and suggestion of the sport exchange between Korea National Sport University(KNSU) and Korea University of Physical Education(KUPE), and the character of both school. And the results are following. The sport exchange between KNSU and KUPE is meaningful compare to the past. Because university students are the main agent of this sport exchange and they are the heros of the future who have nation on their shoulder. Also, this university based sport exchange can be a good model after the past sport exchange between south and north Korea. This sport exchange is expected to enlarge its scale, such as hold an academic seminar or group project, so that it can be a guide which suggest a new direction of exchange between south and north Korea.

일본의 문헌에서 본 스포츠영웅 손기정

하정희(Ha, Jung-Hee),손환(Son, Hwan) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.3

This Study examined Japanese documents, focusing on Berlin Olympic to World War Ⅱ, postwar to Barcelona Olympic and after the Barcelona Olympic to death of Shon Kee Chung, to know how Japanese documents cover Shon Kee Chung. And conclusions are following. First, in Japanese document, it says Shon Kee Chung won a marathon in Berlin Olympic, which fulfilled Japan"s wish in 24 year. Also, we could recognize its intention that they want to consider Shon Kee Chung, who won a first prize and the third each, as Japanese. Second, Japanese document described well how Shon Kee Chung won a marathon and said it fulfilled Japan"s 24 year long desire. Also it described Shon Kee Chung as a Japanese. Third, it mentioned Shon Kee Chung won a marathon, making a spurt, as a Japanese and introduced how Japanese media reported. But unlike before, it mentioned that Shon Kee Chung is a Korean and covered in detail about his life and work.