벼 염기성 chitinase cDNA 유전자의 대장균에의 발현

판철호,임성렬,박노동,김수일 한국응용생명화학회 1994 한국응용생명화학회 학술발표회 Vol.1994 No.1

Secretory Expression of RehmanniaClass III Chitinase in Bacillus subtilis

판철호,김수일 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.3

Anti-inflammatory Effects of Eriodictyol in Lipopolysaccharidestimulated Raw 264.7 Murine Macrophages

판철호,김은선,엄병헌,이재권 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4

Flavonoids have biological activities including anti-allergic, anti-inflammatory, antimicrobial and anticancer activities shown from in vitro studies. Of these biological activities, the antiinflammatory capacity of flavonoids has long been emphasized in Chinese medicine. In this study, I investigated that what flavonoid can be applied to the suppression of lipopolysaccharide (LPS)-induced inflammatory responses in macrophages among the four similar structure flavonoids. Eriodictyol was found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells in non-cytotoxic concentrations. Moreover, eriodictyol strongly suppressed the phagocytic activity of activated macrophages. Pre-treatment of Raw 264.7 cells with eriodictyol reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to be caused by blockage of nuclear factor kappa-light-chainenhancer of activated B cells (NF-κB) activation and phosphorylation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun Nterminal kinase (JNK).

Tectorigenin Inhibits IFN-γ/LPS-induced Inflammatory Responses in Murine Macrophage RAW 264.7 Cells

판철호,김은선,정상훈,노주원,이재권 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.11

Tectorigenin (Tg) and tectoridin (Td) are the major compounds isolated from the rhizomes of iridaceous plant Belamcanda chinensis which is well known as a chinese traditional medicine for the treatment of inflammatory diseases. In this study we investigated whether tectorigenin and tectoridin can be applied to the suppression of interferon-γ and lipopolysaccharide (IFN-γ/LPS)-induced inflammatory responses in macrophages. Anti-inflammatory activities of tectorigenin and tectoridin were compared with genistein (Ge), well known isoflavonoid as a phytoestrogen and regarded as an emerging anti-inflammatory agent. Both compounds showed low cytotoxic effect. In Raw 264.7 cells activated with IFN-γ/LPS, pre-treated tectorigenin was found to inhibit the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the secretion of interleukin (IL)-1β dose-dependently. Tectorigenin also decreased the expression of cyclooxigenase (COX)-2 and the production of prostaglandin E2 (PGE₂) in dose-dependent manner. These inhibitory effects of tectorigenin were found to be caused by the blocking of nuclear factor kappa-B (NF-κB) activation. Compared with genistein and tectoridin, tectorigenin showed significant inhibitory effect for almost anti-inflammatory tests in this study. All these results clearly demonstrated that tectorigenin appears to have the potential to prevent inflammation.

Study on the materialization of microalgal carotenoid

판철호 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

Microalgae are important primary producers in aquatic environments and play a critical role in supporting aquatic animals. Various chemicals, including fatty acids, sterols, phenolic compounds, terpenes, enzymes, polysaccharides, alkaloids, toxins, and pigments, have been isolated and structurally determined from an extensive investigation of microalgae. Carotenoids are biosynthesized by bacteria, algae, fungi, and plants, but not by animals, which must obtain carotenoids from their food. In this study, we focused on the materialization of carotenoids from Chlorella sp. and marine diatoms.

Enzymatic Properties of Recombinant Rehmannia Class III Chitinase Expressed in Bacillus subtilis

판철호,Su-Il Kim 한국키틴키토산학회 2008 한국키틴키토산학회지 Vol.13 No.3

To investigate the enzymatic properties of an acidic class III chitinase of which gene, JHIII, was isolated from Rehmannia glutinosa, the recombinant protein, rJHII, was obtained from the culture of B. subtilis WB600 transformed with JHIII gene. It showed a maximum chitinolytic activity at pH 6 and 50oC, and revealed a relatively high enzymatic activity of more than 50% in the broad range of pH (pH 2 to pH 8) and temperature (20oC to 70oC). The catalytic activity of rJHI was lowered to 85% and 42% in the presence of 5M and 10 M allosamidin, respectively, which is a potent competitive inhibitor of family 18 chitinases. The rJHIII was considered as an endo-type enzyme because it hydrolyzed (GlcNAc)6 into (GlcNAc)₂, (GlcNAc)₃, and (GlcNAc)₄, but did not hydrolyze (GlcNAc)₂-5. The rJHI inhibited the growth of Fusarium oxysporum R2, which was isolated from the root of Rehmannia and identified as a specific fungal pathogen of Rehmannia. The inhibition by rJHIII was a dose-dependent manner.

한국 자생식물 추출물의 항산화 및 Aldo-keto Reductase Family 1 B10 효소 억제 효과

판철호 ( Cheol Ho Pan ),이주영 ( Joo Young Lee ),송대근 ( Dae Geun Song ),김종환 ( Jong Hwan Kim ),안수용 ( Soo Young Ahn ),배덕성 ( Deok Sung Bae ),김영훈 ( Young Hoon Kim ),이재권 ( Jae Kwon Lee ) 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.4

지황 chitin 분해효소의 전기영동 양상 및 분류

판철호(Cheol Ho Pan),이은아(Eun A Lee),조도현(Do Hyun Jo),김수일(Su Il Kim) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7

Chitinase, which is one of the pathogenesis-related proteins, was examined from Rehmannia glutinosa. Rehmannia chitinases were classified into basic and acidic groups by chitin affinity column chromatography. According to the chitinase pattern by native PAGE, basic chitinases of low Rf values were extracted more under an acidic condition (pH 2.9) than a basic condition (pH 8.8), while acidic chitinases having high Rf values were mainly detected in basic extracts. There were in total seven chitinase isoforms of three basic and four acidic isoforms in Rehmannia. The range of molecular weight of Rehmannia chitinase was from 28 to 32 kD. It showed the possibility of tissue specific expression of acidic chitinases and basic chitinases were mainly detected in root.

Mathematical models of pretreatment processes to utilize purple-fleshed potato (Solanum tuberosum L.) peels for anthocyanin extraction

정화빈,판철호,윤원병 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

The effect of particle size on the extraction rate of anthocyanin from Solanum tuberosum L. (purple-fleshed potato) peels (PFPPs) using the stagnant single-stage batch extraction system was investigated. Core pretreatment processes such as drying and grinding were quantitatively evaluated using mathematical models. The drying behavior of PFPP was successfully described using thin layer models (Page model and Midilli-Kucuk model). The effective diffusion coefficient of drying temperature at 40oC was determined to be 1.67x10−12 m2/s. The grinding time to obtain particles of a specific size was accurately estimated using the grinding kinetic model (R2=0.97). The extraction rate of anthocyanin increased as the particle size decreased; however, when the particle size was 0.15mm, the anthocyanin content decreased. Our study demonstrated that the grinding kinetic model is useful to estimate the grinding time to produce an optimum particle size for anthocyanin extraction from PFPP.

유색감자 홍영(Solanum tuberosum L.)의 입자 크기에 따른 반죽과 면의 물리적 특성

정화빈 ( Hwabin Jung ),판철호 ( Cheol Ho Pan ),윤원병 ( Won Byong Yoon ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.3

건강기능성 물질을 함유하며 고유의 색과 향미를 가지는 유색감자 홍영 분말을 첨가한 반죽 및 면의 물리화학적 특성을 확인하였다. 홍영 미세 분말은 ball-milling을 통하여 제조하였으며, 매우 낮은 입자도로 인하여 감자의 세포 및 전분 구조가 파괴되어 손상 전분의 함량이 높았다. 이 같은 손상 전분은 낮은 온도에서 물을 흡수하고 팽창하는 성질이 높아 일반 분쇄를 통하여 얻은 분말로 제조한 반죽에 비하여 BM 분말을 반죽에 첨가하였을 때 탄성이 높고 자유수가 적은 반죽 및 면이 제조되었다. 입자 크기를 달리하여 분쇄한 홍영 분말의 경우 손상 전분이 아닌 첨가 분말의 표면적에 의하여 영향을 받으므로 입자 크기가 클수록 반죽 내의 자유수가 많아 열처리 시 더 많은 호화가 발생하였다. 홍영 분말을 첨가한 경우 홍영 특유의 붉은색이 증가함을 확인하였으며, 홍영 분말의 입자 크기가 커질수록 적색도가 감소하였다. 홍영 분말의 다양한 입자 크기에 따라 손상 전분의 발생 및 입자의 표면적에 의하여 서로 다른 호화 특성 및 면 반죽의 조직감을 나타내었다. 본연구 결과를 통하여 미세 분말(BM)의 경우 손상 전분으로 인하여 생반죽의 탄성이 밀가루에 비하여 매우 높아 성형에 더 많은 힘이 필요함을 확인할 수 있었다. 따라서 미세분말인 BM 분말과 다르게 생반죽의 성형이 용이하면서 홍영 특유의 붉은 색을 가장 잘 나타내며, 조리 이후 반죽의 탄성을 향상시키고 밀가루와 인장력에 유의차가 나타나지 않는 R1 및 R2 분말로 제조한 면이 우수한 품질을 나타냄을 확인하였다. 본 연구 결과는 전분을 함유하는 대체분말을 이용한 제면 제품의 개발 시 입자 크기의 결정에 대한 기반이 될 수 있을 것으로 사료된다. The physical properties of raw and cooked dough for noodle with different sizes of red fleshed potato flour (RFPF) prepared by ball-milling and grinding were investigated. Microstructure images were taken to examine the structure of wheat flour (W) and RFPFs in dough. Thermal and texture properties were measured to investigate the effect of particle size on the physical properties of dough. Thermal properties of flours demonstrated that the highest degree of the damage in starch structure was found in ball-milled (BM) flour. Viscoelastic properties were evaluated using stress relaxation test. The addition of RFPF in dough showed a higher value of elastic components and shorter relaxation time. The increment of the lone spring modulus (Ee) for BM dough was 1346.66% whereas for the largest size of RFPF, the dough was 2543.75%. That different degree in the increment of viscoelastic properties indicates that highly damaged starch in BM showed less gelatinization compared to other RFPFs. The addition of RFPF increased the color parameter a (red-green) of dough and noodle from -3.46±0.17 to 6.08±0.13, respectively. This study suggested that very fine flour rather negatively affected the dough properties, whereas finding a suitable particle size plays an important role in controlling the quality of both dough and noodle.