http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 케이제이쳉 (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
서로 다른 지역에서 분리된 혐기성 곰팡이의 발효특성 비교에 관한 연구
이성실,하종규,케이제이쳉 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5
Four strains of anaerobic gut fungi isolated from different geographical areas were grown at 1, 2, 4, and 6 days on rice straw substrate for comparison of fermentation end-products, substrate utilization and secretion of cellulose and xylanase enzymes. Two of the fungi (Piromyces rhizinflata B157 and Orpinomyces joyonii SG4) were isolated from the fences of bison in England and the rumen of sheep in Canada, respectively, and the other two fungi(Piromyces SS23-2 and Orpinomyces KNGF-2) isolated from the rumen of sheep and Korean native goat, respectively in Korea. The rice straw degradation rate by tested anaerobic gut fungi after 6 days of incubation were estimated 50.54, 31.19 and 58.82 and 53.03% for P. rhizinflata B157, O. joyonii SG4, Piromyces SS23-2 and Orpinomyces KNGF-2, respectively. Two of fungi belong to Piromyces genus secreted the more higher levels of enzymes in a relatively short time than those belong to Orpinomyces genus. Fungi isolated in Korean also secreted the more higher levels of CMCase and xylanase enzymes than those isolated in Canada and England, with statistical difference(P$lt;0.05). Our results indicated that the cellulolysis by anaerobic gut fungi could be different according to the geographical distribution, host animals, isolation parts such as rumen, fences or saliva etc, even though classified and assigned same genus of fungi. The correlation of the diversity of gut fungi with their hosts and geographic areas, their physiology, especially their cellulolytic activities, and their origins and relationships with other organisms should all prove to be fruitful areas for future research.
-
반추위액으로부터 혐기성 미생물(박테리아 , 프로토조아 및 곰팡이)의 분리 방법
신기준,이성실,하종규,박정근,케이제이쳉 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5
Separation methods for rumen microbial fractions contained the bacterial, protozoal and fungal fraction which are the most useful to assess the relative contribution of microbial groups for digestion of feedstuffs and the useful tools to study the rumen microbiology. The fractionation methods have been classified into three main categories; physical method based on cell size and weight, chemical method based on the microbial sensitivity to some chemicals, and physicochemical methods. After microbial groups were selected by physical treatment(centrifuge, filtering and autoclaves), chemical treatment(by the addition of antibiotics and/or some chemicals) and physicochemical treatments of rumen fluid, enumerated each microbial fraction using microscopy and roll-tube methods, and then estimated each separation method. This study emphasized the relative advantageous of the different methods and the factors to be kept in mind when choosing the most appropriate method for a definite goal. Physical treatment(filteration with nylon cloth had larger pore size about 500㎛ or centrifugation) was estimated potentially sufficient to separate protozoal fraction alone from the rumen fluids, due to the phenomenal differences their cell size and weight compared to other microbes, but must be considered of the some kinds of bacteria and/or fungal spores can be survive in protozoa) cell. When separating the microbial groups from the rumen fluids, a physicochemical method is probably the best method, especially when needed separation of bacterial fraction and fungal fraction. And the physicochemical method gave more precise results are presented in Fig. 4 in the main segments of the paper.
-
반추위 혐기성 미생물(박테리아 , 프로토조아 및 곰팡이)이 세포벽 구성물질의 분해에 미치는 상대적 역할
신기준,이성실,하종규,박정근,케이제이쳉 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5
To study the relative contributions of microbial groups (bacteria, protozoa and fungi) in rumen fluids to the overall process of cell wall digestion in the rumen, representatives of these groups were selected by physical treatment, chemical treatment and physicochemical treatments of whole rumen fluids. Physical treatments were performed by centrifuge, filteration and autoclave based on their size and weight. Chemical treatments were also performed by the addition of antibiotics and chemicals to rumen fluids as follows; antibacterial agent[streptomycin sulfate, penicillin G potassium and chloramphenicol(0.010㎎/㎖l each)], antiprotozoan agent[copper sulfate(0.15㎎/㎖), sodium lauryl sulfate (0.010mglml) and dioctyl sulfossucinate sodium salts(0.200㎎/㎖}], and antifungal agent[cychloheximide (0.05㎎/㎖) and nystatin(200U/㎖)]. To evaluate the potential activity and relative contribution to degradation of cell walls by specific microbial groups, the following fractions were prepared: a positive system (whole ruminal fluid), a bacterial (B) system, a protozoan (P) system, a fungal (F) system and a negative system (cell free rumen fluid). To assess the interactions between specific microbial fractions mixed cultures (B+P, B+F, and P+F) were also assigned. Patterns of degradation due to the various treatments resulted in three distinct groups of data based on the degradation rate of cell wall material and on cell wall degrading enzyme activities. The order of degradation was Positive and F systems $gt; B system $gt; Negative and P systems. Therefore, fungal activity was responsible for most of the cell wall degradation. Cell wall degradation by the anaerobic bacterial fraction was significantly less than by the fungal fraction and the protozoan fraction failed to grow under the conditions used. In general, in the mixed culture systems the co-culture systems demonstrated a decrease in cellulolysis compared with the monoculture systems. When one microbial fraction was associated with another microbial fraction, two types of results were obtained. The protozoan fraction inhibited cellulolysis of cell wall material by both the bacterial and fungal fraction, while in the coculture between the bacterial fraction and fungal fraction, a synergistic interaction was detected.
-
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
