Polyacrylamide Gel 전기영동법에 의한 토끼의 각 조직에서 본 수용성 단백의 비교연구

최흥민,김지식,우세홍,한양일 최신의학사 1973 最新醫學 Vol.16 No.5

The soluble proteins pattern in the brain, liver, pancreas, spleen, kidney, testis and ovary of rabbits were separated by mean polyacrylamide gel electrophoresis, and it's soluble proteins pattern were compared. In male rabbits tested, soluble protein fractions of the brain, liver, pancreas, spleen, kidney and testis exhibited 15, 16, 9, 14, 14 and 13 respectively. In female rabbits tested, soluble protein fractions of the brain, liver, pancreas, spleen, kidney and ovary exhibited 15, 16, 9, 14, 15 and 10 respectively. In female rabbits tested, it's kidney was shown that one more fraction appeared in' male kidney, and other tissues similar to male, except reproductive organs.

夏節農村人과 正常人의 血液像比較

최흥민,윤기은,한양일,나동진 대한임상병리사협회 1973 대한임상병리사회지 Vol.5 No.1

Hematological normal values of 255 Korean children including number of WBC, hemoglobin, hematocrit, were studied. Mean values according to age and sex were obtained. Results are as table V.

韓國産 貝類의 Hemolymph에 대한 Disc-電氣泳動橡의 比較硏究(Ⅰ)

최흥민,우세홍,한양일,나동진 최신의학사 1974 最新醫學 Vol.17 No.3

韓國産 海産貝類 8種의 hemolymph에 대하여 disc-電氣泳動한 結果 개조개 9개, 꾜막7개, 바지락8개, 동죽6개, 홍합4개, 콩조개6개 남방재첩 9개. 대합7개의 泳動帶를 가진 泳動像을 얻을 수 있었고 各各의 特徵에 따른 差異點을 發見할 수 있었다. 이것이 더 나아가 外形을 分別할 수 없을 때의 種을 鑑別하여 낼 수 있는 하나의 factor가 될 수 있다. The identify of a Pelecipoda is usually readily determined from the physical appearance of the Pelecipoda. The present objective methods of identifying Pelecipoda is based on the species-specific protein separation patterns obtained on electrophoresis of the hemolymph of the Pelecipoda. This is also can be separated by electrophoresis into species characteristic patterns that could be used for species identification. The separation patterns obtained on polyacrylamide gel for the hemolymph of fresh Saxidomus purpuratus, Tagillarca granosa, Tapes philippinarum, Mactra venerifomis, Mytilus edulis, Corbicula felnouilliana, Coricula fluminea, Meretrix lusoria are presented.

흰쥐 및 생쥐의 各器官에 있어서 Esterase Isozyme의 比較硏究

최흥민,김지식,박정오 최신의학사 1973 最新醫學 Vol.16 No.3

Rat. Mouse의 brain, liver, pancreas, spleen, heart 그리고 kidney 에서 Esterase isozyme 을 agar gel 을 使用한 Thin layer electrophoresis 로 그 分劃像을 比較하여 보았다. Rat에서는 kidney를 除外한 모든 器官에서 male과 female이 共히 brain에서 4, liver에서 4, pancreas에서 3, spleen 에서 3, heart에서 3個의 泳動帶가 나타났고 female kidney 에서는 5個의 泳動帶가 分離되었다. Mouse에서도 kidney를 除外한 다른 모든 器官에서 male과 female이 共히 brain에서 3, liver에서 5, pancreas에서 3, spleen에서 3, heart에서 3個의 泳動帶가 나타났고 male kidney 에서는 4個 , female kidney에서는 5個의 泳動帶가 分離되어 나타났다. 以上의 結果로 보아 同一個體에 있어서는 器官別로 다른 Esterase isozyme이 나타났고 同一種에 있어서 brain, liver, pancreas, spleen, 그리고 heart에서는 성별에 관계없이 동일한 Esterase isozyme을 볼수 있었으며 Kidney에서만 male보다 female이 cathode쪽으로 한개의 Band가 나타나 性別差異를 볼 수 있었다. 또한 異種사이에서는 同一한 器官이라도 Esterase isozyme의 差異를 볼 수 있었다. 泳動帶의 mobility를 特徵別로 구분하여 본 結果 Esterase isozyme이 6 group으로 區別됨이 本實驗에서 注目할만한 點이라 하겠다. The esterase isozyme in the brain, liver, pancreas, spleen, heart and kidney of Rat, Mouse were separated by means of Agar gel electrophoresis, and their esterases were compared. In male Rat tested. Esterase isozyme of the brain. liver. pancreas, spleen, heart and kidney exhibited 4, 4, 3, 3, 3, and 4 bands each other. In female Rat tested, it's kidney was shown that one more band appeared in male kidney, and other organs similar to male. The male mouse esterase isozyme of the brain, liver, pancreas, spleen, heart and kidney exhibited 3, 5, 3, 3, 3 and 4 bands each other. In female mouse tested, it's kidney was shown that one more band appeared in male kidney, and other organs similar to male. The result of experiment, the bands were classified into six groups.

미취학 아동의 적혈구 엽산치에 관한 연구

최흥민,김태전 최신의학사 1978 最新醫學 Vol.21 No.6

The present study was carried out to establish the levels of red cell folate as a better basic data to appraise the untritional stational status of folic acid in normal infants and children (1-7 ages). Blood sample was collected from 10 healthy infants and 60 children who visited or were adimitted to the Severence hospital, Yonsei university college of Medicine from Aug. 1975 to Apr. 1976. The folate assay was done with modified Cooperman's method, using Lactobacillus casei ATCC 7469. The results obtained were as following. 1. In infant, the level of red cell folate was 474.8:±94.2ng/ml with range 160.2-1091.2ng/mL 2. In children. the level of red cell folate was 252.3±12.3ng/ml with range 91.0-620.3ng/ml. 3. The level of red cell folate in infants was significantly higher than that in children and adults (P 0.005) 4. There were no significant difference on the sex of pre-school children. As the results of the above, the level of red cell folate was considered along with age in indicating the nutritional status of folic acid in normal infants and children. It was suggested that the number of subjects was inadequate in this study, and in future study the more subjects should qe measured to get the better criterion of red cell folate for pre-school children.

ACTH投與에 따른 白鼠의 1日中 名種 白血球의 變動像硏究

최흥민,한양일,나동진 최신의학사 1974 最新醫學 Vol.17 No.3

A survey was carried out in order to figure out of phenomenon of increasing and decreasing rate of WBC during a day in normal and healthy rat by injection ACTH. For this survey, it was classified by two groups 20 male rat and 20 female rat that are 8 month old. This blood sampling was collected by thin smear method 10 times from 10:30 A. M. to next 8:30 A. M. by 2 hours intervals and calculated by direct manual method. As the results of this survey, the following conclusions were obtained. 1. Increase and decrease of neutrophil and lymphocyte was almost same with control. 2. Eosinophil was decreased after two and four hours by injected ACTH.

하루중 정상인의 백혈구수 변동상 조사

최흥민,이명화,구성회,한식일,나동진 한국환경보건학회 1974 한국환경보건학회지 Vol.1 No.1

A survey was carried out in order to figure out of phenomenon of increasing and decreasing rate of WBC during a day in normal and healthy person from Oct. 1, 1972 to Nov. 31, 1972. For this survey, it was classified by two groups 50 male students and 35 female students who are from 18 to 25 years old. The blood sampling was collected by thin smear method 8 times from 7:30 A.M. to 10:30 P.M. by 2 hours intervals and calculated by direct manual method. As the results of this survey, the following conclusions were obtained 1) Number of WBC was the lowest degree during the forenoon, and it was the highest degree around the midnight. It was increase from around the noon up to the midnight and decrease from the midnight up to around the noon. 2) Increasing and decreasing curve of WBC of male and female was keeping same pace with both sex in a day. 3) Increase and decrease of lymphocyte and eosinophil was keeping almost same pace with WBC. 4) Increasing rate of number of monocyte was more speedily increasing during the afternoon than the night.

夏節農村人과 正常人의 血液像比較

崔興敏,尹基殷,韓良一,羅東進 대한임상병리사협회 1973 임상병리사회지 Vol.5 No.1

Indomethacin으로 유발된 생쥐의 위 점막 손상에 대한 향사평위산(香砂平胃散)의 보호 효과

최흥민,임성우,Choi, Heung-Min,Lim, Seong-Woo 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.3

This study was carried out to investigate the effects of Hyangsapyeongwisan(HP) on gastric mucosal lesions induced by indomethacin in mice. The control group consisted of gastro-inflammation elicited mice. The sample group consisted of mice given HP after onset of gastro-inflammation. In common morphological and histochemical change, various cell abnormalities were observed in the control group, such as mucous surface cell, peanut cell, surface epithelial cell, goblet cell abnormalities, all caused hemorrhagic erosion. The sample group was the same as the control group. In the immunohistochemical change, the distributions of COX-I, BrdU treated with HP were notably higher than those of the control group(p<O.05). The distributions of TUNEL, $NF-{\kappa}B$, COX-2, PKC, IL-12B in mice treated with HP were notably lower than those of the control group(p<O.05). The above results support a role for Hyangsapyeongwisan in treating gastric mucosal lesions.

正傳加味二陳湯이 Dibutyltin Dichloride(DBTC)로 유발된 흰쥐의 췌장염에 미치는 영향

최흥민(Heung-min Choi),김학재(Hak-jae Kim),한이수(Yi-soo Han),임성우(Seong-woo Lim) 대한한의학회 2007 대한한의학회지 Vol.28 No.3