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Saucernetin-8 from Schisandra chinensis as a Potent Antiviral Agent Against Coxsackievirus B3
최화정 대한미생물학회 2024 Journal of Bacteriology and Virology Vol.54 No.1
Various plants have been studied for their therapeutic potential to treat virusinduced diseases. This study aimed to investigate in vitro anti-coxsackievirus B3 (CVB3) effect of saucernetin-8 isolated from Schisandra chinensis and mode of its action. The action of saucernetin-8 on CVB3 proliferation, the effect of saucernetin-8 on VP3 protein, mitochondrial reactive oxygen species (mtROS) and expression levels of STING/p-STING/IRF3/p-IRF3 were investigated by RT-qPCR, immunofluorescence and Western blot. Saucernetin-8 exhibited over 60% inhibition of CVB3-induced cytotoxicity at a concentration of 0.4 μg/mL. Saucernetin-8 completely suppressed viral genome replication at a concentration of 10 μg/mL. Furthermore, saucernetin-8 inhibited the expression of VP3, the capsid protein of CVB3, by approximately 50% at a concentration of 10 μg/mL. Immunofluorescence assay showed that saucernetin-8 inhibited VP3 expression by approximately 50%. Saucernetin-8 increased mtROS levels in CVB3-infected Vero cells. The STING/IRF3 pathway is activated in cells treated with saucernetin-8. Therefore, saucernetin-8 is a candidate for development of antivirals against CVB3.
Evaluation of Antiviral Activity of Zanthoxylum Species Against Picornaviruses
최화정 질병관리본부 2016 Osong Public Health and Research Persptectives Vol.7 No.6
Human rhinoviruses and enteroviruses (family Picornaviridae) infect millions of people worldwide each year, but little is known about effective therapeutical treatment for the infection caused by these viruses. We sought to determine whether or not Zanthoxylum (Rutaceae) species can exhibit antiviral activity against picornaviruses. The leaf parts of four Zanthoxylum species were extracted with methanol, and the extracts were investigated for their antiviral activity against picornaviruses using cytopathic effects by cytopathic effect reduction. Leaf extracts of Zanthoxylum piperitum among four Zanthoxylum species were found to possess only broad-spectrum antipicornavirus activity against human rhninovirus 2 with a 50% inhibitory concentration (IC50) value of 59.48 ㎍/mL, human rhinovirus 3 with an IC50 value of 39.94 ㎍/mL, coxsackie A16 virus with an IC50 value of 45.80 ㎍/mL, coxsackie B3 virus with an IC50 value of 68.53 ㎍/mL, coxsackie B4 virus with an IC50 value of 93.58 ㎍/mL, and enterovirus 71 virus with an IC50 value of 4.48 ㎍/mL. However, ribavirin did not possess antiviral activity against human rhinovirus 3 and four enteroviruses. Therefore, leaves of Z. piperitum showed broad-spectrum antipicornavirus activity, and may be useful as a candidate for studying picornavirus agents and development of pharmaceuticals.
In Vitro Antiviral Activity of Sakuranetin against Human Rhinovirus 3
최화정 질병관리본부 2017 Osong Public Health and Research Persptectives Vol.8 No.6
Objectives: Rhinoviruses (RVs) cause common cold and are associated with exacerbation of chronic inflammatory respiratory diseases. Until now, no clinically effective antiviral chemotherapeutic agents to treat diseases caused by human rhinoviruses (HRVs) have been reported. We assessed the anti-HRV3 activity of sakuranetin isolated from Sorbus commixta Hedl. in human epithelioid carcinoma cervix (HeLa) cells, to evaluate its anti-rhinoviral potential in the clinical setting. Methods: Antiviral activity and cytotoxicity as well as the effect of sakuranetin on HRV3-induced cytopathic effects (CPEs) were evaluated using the sulforhodamine B (SRB) method using CPE reduction. The morphology of HRV3-infected cells was studied using a light microscope. Results: Sakuranetin actively inhibited HRV3 replication and exhibited antiviral activity of more than 67% without cytotoxicity in HeLa cells, at 100 μg/mL. Ribavirin showed anti-HRV3 activity similar to that of sakuranetin. Treatment of HRV-infected HeLa cells with sakuranetin visibly reduced CPEs. Conclusion: The inhibition of HRV production by sakuranetin is mainly due to its general antioxidant activity through inhibition of viral adsorption. Therefore, the antiviral activity of sakuranetin should be further investigated to elucidate its mode of action and prevent HRV3- mediated diseases in pathological conditions.
Inhibitory Effects of Norwogonin, Oroxylin A, and Mosloflavone on Enterovirus 71
최화정,송혁환,이재숙,고현정,송재형 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.5
Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.
최화정,이재훈,여선주,Kusuma Kaewintajuk,이규양,김석,송현옥,박현 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5
Coccidiosis affects many vertebrates worldwide,but treatment with known anti-coccidial drugs causesseveral adverse side effects. There is a critical need for thedevelopment and evaluation of new drugs. The anti-coccidialeffect of 1-[4-(4-nitrophenoxy)phenyl]propane-1-one(NPPP), a synthetic compound, was studied in vitro andin vivo. Treatment with NPPP showed anti-Toxoplasmaactivity in vitro with a lower EC50 value than pyrimethamine. In ICR mice infected with Toxoplasma gondii, oraladministration of NPPP for 4 days showed statisticallysignificant anti-Toxoplasma activity with lower numbers oftachyzoite than those of the negative control (p\0.01). NPPP also exhibited strong anti-Eimeria activity in Eimeriatenella-infected chickens when treated for 4 days withorally administered NPPP at a dose of 100 mg/kg. Potentialtarget proteins of NPPP were analyzed by proteomic profilesof T. gondii tachyzoites. Two hypothetical proteinswere identified as possible targets of NPPP, a putativeortholog of vacuolar ATP synthase subunit C and a class IS-adenosylmethionine-dependent methyltransferase. Ourdata show that the NPPP might be an anti-coccidial drugcandidate for clinical application against coccidial infections. Future investigations will focus on identifying thefunction of proteins regulated by NPPP.
어성초로부터 분리된 Quercetin의 카드뮴에 대한 독성억제효과
최화정,노용주,강정일,백승화,Choi, Hwa-Jung,No, Yong-Ju,Kang, Jeong-Il,Baek, Seung-Hwa 대한약학회 2009 약학회지 Vol.53 No.1
The aim of this study was to develop antitoxic compound for cadmium-induced cytotoxic Vero cells. These cells were divided into five groups; control group (medium only), cadmium group (cadmium only), and two experimental groups. SRB (sulphorodamine B) assay was performed to evaluate the cytotoxicity of cell organelles. After cadmium was treated on Vero cells, we determined IC50 values to examine the detoxification effects of Houttuynia cordata methanol extract and quercetin under cadmium-induced cytotoxicity. Furthermore, morphological changes were observed by the light microscope. In Vero cells, methanol extract of Houttuynia cordata, and quercetin showed inhibitory effects on cadmium-induced cytotoxicity and these detoxification effects were increased in a concentration-dependent manner. These results suggest that methanol extract and quercetin from Houttuynia cordata retain a potential antitoxic activity.