http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 최차용 (검색결과 143 건)
- 무료
- 기관 내 무료
- 유료
-
최차용,박홍우 한국화학공학회 1981 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.19 No.6
PDP 11-03마이크로 콤퓨터를 발효 시스템에 성공적으로 연결하고 개스 분석기를 주된 측정 기기로 사용하여 효모 Saccharomyces cerevisiae의 배양을 수행하였다. 필요한 방정식과 콤퓨터 프로그램을 개발하여 시스템 운전에 사용하였다. 최적 휘드 백 정책을 콤퓨터 프로그램하여 콤퓨터에 기억시킨 후 측정치를 사용하여 적절한 값의 제어시그날을 발생시키도록 하였다. 간접적으로 얻은 균체농도는 직접 측정치를 상당히 정확하게 예측하였다. 온 라인 휘드 백 제어를 수행한 결과 얻어진 호흡지수는 아주 흡족한 상태를 보였으며 전반적인 세포 생산 수율은 회분 배양과 비교했을 때 상당히 증가되었다. A PDP 11-03 microcomputer was successfully interfaced to the fermentation system and yeast Saccharomyces cerevisiae cultivation with this computerized system was carried out using the gas analyzers as the primary measuring elements. The necessary equations and computer programs were developed and used in the system operation. The optimal feed-back control policy computerized and stored, in the system utilizes these monitored variables in order to generate the output control signals at proper level. The indirectly determined cell-concentration has predicted the direct measurement rather accurately. The profile of the respiratory quotient obtained upon exercising the on-line fee-back control has shown a very smooth and desirable behaviour and the ob erall cell yield has been improved quite significantly as compared with the batch system without control.
-
-
-
-
Minimally Complex Problem Set for an Ab Initio Protein Structure Prediction Study
최차용,RyangGug Kim 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.5
A minimally complex problem set for ab initio protein structure prediction has been proposed. As well as consisting of non-redundant and crystallographically determined high-resolution protein structures, without disulphide bonds, modified residues, unusual connectivities and heteromolecules, it is more importantly a collection of protein structures, with a high probability of being the same in the crystal form as in solution. To our knowledge, this is the first attempt at this kind of dataset. Considering the lattice constraint in crystals, and the possible flexibility in solution of crystallographically determined protein structures, our dataset is thought to be the safest starting points for an ab initio protein structure prediction study.
-
-
Construction of CpG Motif-enriched DNA Vaccine Plasmids for Enhanced Early Immune Response
최차용,Young Seoub Park,Seung Ha Hwang 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1
A DNA vaccine methodology using eukaryote expression vectors to produce immunizing proteins in the vaccinated hosts is a novel approach to the development of vaccine and immuno-therapeutics, and it has achieved considerable success over several infectious diseases and various cancers. To further enhance its efficiency, attempts were made to develop novel plasmid vectors containing multiple immunostimulatory CpG motifs, for rapid and strong immune response. First, a 2.9 kb compact plasmid vector (pVAC), containing CMV promoter, polycloning site, BGH poly(A) terminator, ampicillin resistance gene and pBR322 origin was constructed. A pVAC-hEPO was also constructed, which contained a human erythropoietin gene, for evaluating the transfection efficiency of naked plasmid DNA both in vitro and in vivo. To examine the adjuvant effect of multi-CpG motifs on naked plasmid DNA, 22 and 44 enriched and unmethylated CpG motifs were introduced into pVAC to generate pVAC-ISS1 and pVAC-ISS2, respectively. 100 g of pSecTagB, pVAC, pVAC-ISS1 or pVAC-ISS2 were each injected intramuscularly into the tibilias anterior muscle of Balb/c mice. The level of interleukin-6 induced in the mice injected with pVAC-ISS1 and pVAC-ISS2 were significantly elevated after 12 hours, which were almost 2 and 2.5 times higher than that in the mice injected with pSecTagB, respectively. These results suggest that DNA vaccine plasmids with enriched CpG motifs can induce rapid secretion of interleukin-6 by lymphocytes. In conclusion, these vectors can contribute to the development of adjuvant-free DNA vaccinations against infectious diseases and various cancers.
-
-
크로마토포아와 NAD Kinase 로 이루어진 공역반응계의 모형화와 수치모사
최차용,박두홍 한국화학공학회 1982 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.20 No.2
크로마토포아와 NAD kinase로 구성된 공역 반응계에 대하여 수학적 모형화와 콤퓨터 수치모사를 수행하였다. 크로마토포아에 대해서는 경험적인 반응 속도식을 사용하였으며 공역 반응계의 다른 촉매 성분에 대해서는 이론적으로 유도된 식들을 사용하였다. 크로마토포아와 NAD kinase로부터 완전히 분리하기가 어렵기 때문에 마이오 카이네이즈도 반응계의 구성 성분으로 포함시켰다. 반응계의 촉매 성분들의 상대적인 활성 비율이 NADP 생산에 미치는 영향도 조사되었다. 반응계의 여러 생화학 성분들의 시간에 따른 변화 추이도 수치모사 결과로부터 알 수 있었다. 실험 데이타와 콤퓨터 수치모사 결과를 비교하여 보았다. Mathematical modeling and computer simulation were carried out on the coupled reaction system compreised of chromatophore and NAD kinase. An empirical kinetic model was used for the chromatophore whereas analytically derived models were employed for other catalytic components of the system. Myokinase was also included as a system component because of the difficulty in its complete separation from the chromatophore and NAD kinase. The effect of the relative ratio of the catalytic activities of the system on the overall production of NADP was studied. The time course behaviour of the biochemical species of the system was also obtained from the simulation results. Experimental data and the results of the computer simulation were compared.
-
최차용,임동준 한국화학공학회 1984 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.22 No.4
칼슘 알지네이트 담체에 Candida lipolytica MX 9-11 R 3세포를 포괄시켜 고정화 효모 세포를 만들었다. 유동층 회분 반응기에서 고정화 효모 세포를 사용하여 포도당으로 부터 시트르산 생성에 관한 연구를 하였다. 세포량 증가에 필요한 다른 영양소는 넣지 않고 포도당만 사용함으로써 세포 증식을 다소 막을 수 있었다. 유동층 회분 반응기에서 시트르산 생산성은 반응온도, pH, 공기 유량속도에 크게 영향을 받았다. 시트르산 생산의 최적 조건은, 균일계에서는 26℃(Tabuchi), 30℃(Nakanishi)와 pH 5(Marchal), pH6.5(Nakanishi)였으나, 여기서는 28℃, pH6이었다. 공기 유량속도를 증가시키면 시트르산 생산이 증가되었는데 이것은 용존 산소의 증가와 물질전달 저항의 감소에 기인한 것 같다. 유동층 회분 반응기에 공급되는 공기중에 CO₂를 어느정도 혼입시켰을 때 시트르산의 생산성이 증가하였다. 최적 CO₂의 함량은 16%(V/V)였다. Immobilized yeast cell (Candida lipolytica MX 9-11 R3) was prepared by entrapping the whole cell in calcium alginate matrix. The fermentative production of citric acid from gulcose was studied using this immobilized yeast cell in a fluidized-bed batch reactor. One could more or less prevent the cell growth using glucose as the sole nutrient and thus eliminating other nutrients necessary for the cell mass doubling. The productivity of citric acid was remarkably influenced by reaction temperature, pH, and air flow rate in fluidized-bed batch reactor. The optimal conditions for the citric acid production were found to be 28℃ and pH 6 as compared with those of homogeneous case, i.e. 26℃ (Tabuchi), 30℃ (Nakanishi), and pH 5 (Marchal), pH 6.5 (Nakanishi). The increased air flow rate resulted in an enhanced citric acid production possibly due to the increased dissolved oxygen concentration and the decrease in mass transfer resistance. The intentional addition of CO₂ gas into the air supply to the fluidized-bed batch reactor gave a better citric acid productivity in certain concentration ranges. The optimal CO₂ content was found to be 16% (v/v),
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
