만성 저온 스트레스 동물모델에서의 황련(黃連)의 항염증 효능 연구

최진규 ( Jin Gyu Choi ),허유진 ( Eugene Huh ),이원일 ( Wonil Lee ),김윤경 ( Yun-kyung Kim ),이태희 ( Tae-hee Lee ),오명숙 ( Myung Sook Oh ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6

Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not. Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 ㎎/㎏, (4) CCS+CRE 300 ㎎/㎏, (5) CCS+CRE 1,000 ㎎/㎏ groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at 4℃ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus. Results : Oral administration of CRE 1,000 ㎎/㎏ significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS. Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.

복신(茯神)의 인지기능 향상 및 해마 신경세포분화 촉진에 대한 효능 연구

최진규 ( Jin Gyu Choi ),심여문 ( Yeo Moon Sim ),김원남 ( Won Nam Kim ),김선여 ( Sun Yeou Kim ),오명숙 ( Myung Sook Oh ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.2

Objectives : The aim of this study was to investigate the memory enhancing properties of extract of Hoelen Cum Radix (HCR) and its possible mechanism in mice of normal condition. Methods : We evaluated the effects of HCR on cognitive function and memory enhancement in normal mice. Male ICR mice were orally administrated with HCR 100 mg/kg for 7 days and equal volume of saline was administrated to the control group in the same condition. We conducted two behavioral tests which measure the spatial working memory (Y-maze test) and cognitive fear memory (passive avoidance test). We also investigated whether HCR affects the hippocampal neurogenesis in the brain. To assess the effects of HCR on neural progenitor cell differentiation and neurite outgrowth in the early stage of hippocampal neurogenesis, we performed doublecortin (DCX), a direct neurogenesis marker, immunohistochemical analysis in the dentate gyrus (DG) of the mouse hippocampus. Results : HCR significantly enhanced memory and cognitive function as determined by the Y-maze test ( p<0.05) and passive avoidance test ( p<0.001). Moreover, HCR increased DCX positive cells ( p<0.01) and neurite length ( p<0.01) compared to the control group. These results indicated that HCR stimulates differentiation of neural progenitor cells and promotes neurite outgrowth in hippocampal DG of the mice. Conclusion : We concluded that HCR shows memory enhancing effects through the stimulation of hippocampal neurogenesis as a consequence of accelerated neuronal differentiation and neurite outgrowth in the DG of the hippocampus after HCR treatment.

나선 맞춤을 이용한 단백질의 3차원 구조 추출

최진규(Jin Gyu Choi),안성준(Sung Joon Ahn) 한국지능시스템학회 2011 한국지능시스템학회 학술발표 논문집 Vol.21 No.1

생명 정보학 분야에서 단백질의 구조에 대한 연구는 매우 중요한 분야 중에 하나이다. 컴퓨터 환경의 발전에 따라 단백질 정보는 데이터베이스화 되어 보급되고 있으나, 이를 분석하기 위해서는 여전히 사람이 직접 비교하는 방법을 사용하고 있다. 이를 극복하고자 본 논문에서는 단백질 구조를 3차원 패턴으로 접근해 보고자 한다. 단백질의 2차 구조, 그 중에서도 가장 흔히 나타나는 나선 구조를 단백질 원자의 좌표로부터 나선 맞춤을 이용하여 추출한다. 그리고 비교 분석을 위해서 그 것을 2차원 맵으로 표현하는 방법을 제안한다.

풍하중 저감형 방음판의 실증 연구

최진규(Jin-Gyu Choi),이찬영(Chan-Young Lee) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.12

최근 도시개발과 생활수준의 향상으로 인해 교통량이 크게 증가하고 있으며, 이와 더불어 증가되는 도로 소음으로 인해 많은 민원이 제기되고 있다. 이에 대한 대책으로 도로변에 높은 방음벽이 설치되고 있으나 방음벽 주요 설계 요건인 풍하중은 공사비의 기하급수적인 증가뿐만 아니라 방음벽 높이 제한의 요인으로 작용하게 된다. 이에 본 연구에서는 기존 방음벽 수준의 차음 성능과 더불어 풍하중을 획기적으로 저감할 수 있는 우수한 가격 경쟁력의 방음벽을 개발 하는 것을 목적으로 한다. 본 연구 대상의 방음판은 헬름홀츠의 공명기 이론을 바탕으로 공기와 같은 유체는 통과하고 소음은 저감할 수 있는 신개념 통기형 방음판에 해당된다. 본 연구에서는 실 크기의 금속재 방음판을 제작하여 음압투과손실실험, 풍동실험 및 재료품질 실험을 수행하여 고속도로의 품질기준을 만족하는 결과를 도출하였다. 또한 신뢰성을 검증하기 위해 현장에 제작 및 설치를 하고 시간대 별로 소음을 측정하여 방음판의 소음 차단 효과를 확인하였다. 향후 도로에 통풍형 방음벽을 설치할 경우 높은 소음 차단 효과로 인하여 쾌적한 생활환경을 조성할 수 있을 것으로 예상된다. Traffic volume has been greatly increasing due to urban development and the improvement of living standards, and many complaints are being raised due to the increasing road noise. As a countermeasure against these problems, highly soundproof walls are installed on the sides of roads. However, the ability to bear wind loads is a major design requirement for soundproof walls, which contributes to the exponential increases in construction costs and restricts the height of the walls. The aim of this study is to improve the performance of soundproof walls and to dramatically reduce wind loads while maintaining excellent price competitiveness. Based on Helmholz"s resonator theory, a new concept is proposed for a ventilation-type soundproofing plate that can pass through a fluid like air and reduce noise. A full-scale metal soundproofing plate was produced to satisfy the quality standards of highways by conducting a sound-pressure transmission-loss test, wind tunnel test, and material quality test. To verify the reliability, the wall was manufactured and installed, and the sound insulation effect was examined by measuring the noise over time. In the future, ventilated soundproof walls on roads could create a pleasant living environment due to the high noise-insulation effect.

페일 세이프 코드의 성형가공 롤 포밍 머신의 개발

최진규(Jin-Gyu Choi),이동욱(Dong-Wook Lee),박민혁(Min-Hyeok Park),남광식(Kwang-Sik Nam),조상(Zhao Shang),이석순(Seok-Soon Lee) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.5

Roll Forming technology has a problem forming components by depending on only experiences without accurate data in actual field. To solve the problem, its necessary to analyze FEM of Roll Forming process. Main function of Roll Forming Tool is continuous bending work of materials thickness with few changes. Therefore, this research did a comparative study between FEM result of aluminum plate formed by Roll_Forming Tool and its experiment. FEM result shows that plastic deformation of aluminum plate can be practicable and it is similar to the result of thickness experiment. In addition, design of automatic roll forming machine helped forming process.

심전도와 마이크 센서를 이용한 맥파전달시간 측정

최진규(Jin-Gyu Choi),강영환(Young-Hwan Kang),박재순(Jae-Soon Park),정연호(Yeun-Ho Jeong),구치완(Chi-Wan koo) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.6

휴대폰카메라와 디지털카메라의 화소의 헤도닉가격비교

최진규 ( Jin Gyu Choi ),박두수 ( Doo Soo Pak ),강임호 ( Im Ho Kang ) 정보통신정책학회 2009 정보통신정책연구 Vol.16 No.4

인진호(茵蔯蒿)와 한인진(韓茵蔯)의 추출용매별 항염증 효능 비교

노동진 ( Dongjin Noh ),최진규 ( Jin Gyu Choi ),홍순선 ( Soon-sun Hong ),오명숙 ( Myung Sook Oh ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.3

Objectives : Artemisia capillaris Thunberg (AC) and Artemisia iwayomogi Kitamura (AI) have been used without distinguishment since ancient times due to similar appearance. In this study, we compared the inhibitory effects of AC and AI on the expression of inflammatory cytokines induced by lipopolysaccharide (LPS) in murine macrophages. Methods : AC and AI were extracted by reflux with distilled water (DW) and 70% ethanol (EtOH). We investigated the inhibitory effects of AC and AI on the expression of nitric oxide (NO), inducible NO synthase (iNOS) and tumor necrosis factor-α (TNF-α) induced by LPS in macrophages. Results : Firstly, yield of the samples was higher in order of Artemisia iwayomogi DW Extract (AID), Artemisia iwayomogi 70% EtOH Extract (AIE), Artemisia capillaris DW Extract (ACD) and Artemisia capillaris 70% EtOH Extract (ACE). All of the samples were not toxic in macrophages. The inhibitory effect of the samples on LPS-induced NO expression was stronger in the order of AIE, ACE, AID and ACD. The inhibitory effect of the samples on LPS-induced inducible iNOS expression was stronger in the order of AIE, ACE and AID. Effect of ACD was same with that of AID. In addition, inhibitory effect of the samples on LPS induced TNF-α expression wes stronger in the order of AIE, ACE, AID and ACD. Conclusion: These results showed that AI would be more effective than AC and 70% EtOH would be more effective than DW as an extraction solvent in inflammatory diseases.

선박 용접 소조립에 사용될 절단 철판 인식 기술

김성구(Kim Sung Goo),최진규(Choi Jin Gyu),박제호(Park Jea Ho),안성준(Ahn Sung Joon) 한국통신학회 2023 한국통신학회 학술대회논문집 Vol.2023 No.6

꼬시래기 홍조류로부터 열산가수분해, 효소당화 및 에탄올 발효

나채훈 ( Chae Hun Ra ),최진규 ( Jin Gyu Choi ),강창한 ( Chang Han Kang ),선우인영 ( In Yung Sun Woo ),정귀택 ( Gwi Taek Jeong ),김성구 ( Sung Koo Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2015 한국미생물·생명공학회지 Vol.43 No.1

본 연구는 해조류, 꼬시래기를 발효하여 에탄올을 생산하였다. 최적 전처리 조건은 12% (w/v) 해조류 슬러리, 270 mM 황산, 121도60분동안 실시하였다. 열산가수분해 후에, 꼬시래기 가수분해산물에 16 U/ml의 혼합효소 Viscozyme L과 Celluclast 1.5 L를 이용하여 효소당화를 수행하였다. 50.4 g/l의 총 단당류의 농도는, 120 g dw/l 꼬시래기 슬러리로부터 열산가수분해와 효소당화에 의해 총 탄수화물 60 g/l의 전환율 84.2%를 나타내었다. 꼬시래기 가수분해산물은 분리당화발효(SHF)로 에탄올 생산을 위한 기질로 사용하였다. 고농도 galactose로 순치한 Candida lusitaniae ATCC42720에 의한 에탄올 생산은 0.43의 에탄올 수율(YEtOH)인 22.0 g/l를 생산하였다. 특정 당에 순치한 효모는 혼합당의 흡수에 유용하며, 그 결과 해조류 가수분해산물 배지로부터 높은 에탄올 수율을 나타내었다. The seaweed, Gracilaria verrucosa, was fermented to produce bioethanol. Optimal pretreatment conditions were determined to be 12% (w/v) seaweed slurry and 270 mM sulfuric acid at 121oC for 60 min. After thermal acid hydrolysis, enzymatic saccharification was carried out with 16 U/ml of mixed enzymes using Viscozyme L and Celluclast 1.5 L to G. verrucosa hydrolysates. A total monosaccharide concentration of 50.4 g/l, representing 84.2% conversion of 60 g/l total carbohydrate from 120 g dw/l G. verrucosa slurry was obtained by thermal acid hydrolysis and enzymatic saccharification. G. verrucosa hydrolysate was used as the substrate for ethanol production by separate hydrolysis and fermentation (SHF). Ethanol production by Candida lusitaniae ATCC 42720 acclimated to high-galactose concentrations was 22.0 g/l with ethanol yield (YEtOH) of 0.43. Acclimated yeast to high concentrations of specific sugar could utilize mixed sugars, resulting in higher ethanol yields in the seaweed hydrolysates medium.