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가미부정증효방의 조직손상 회복 및 항산화작용 작용을 통한 방사선 부작용 감소효과
최주선,이은옥,김동희,임낙철,김성훈 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5
This study was attempted to investigate the radioprotective effects of Gamibujungjunghyo-bang(GBJJHB) after in vitro and in vivo irradiation with x-ray. The protection effect on normal cells and anti-tumor enhancing effect through cytotoxicity on A549 cancer cells after exposure to irradiation were not significantly increased by GBJJHB compare with control. GBJJHB prevented weight loss after exposure to irradiation and rather induced weight gain. Spleen index was not changed by GBJJHB compared with control. WBC and platelet were significantly increased in GBJJHB treated group as compared with control group after irradiation by Liniac. In histotological changes of intestine, jejunum and ileum of BALB/C mice after exposure to irradiation, expoliation and fusion of villi were decreased and crypt length was significantly maintained longer in GBJJHB treated group as compared with control. There was a significant increase in the number of crypts labeled with BrdU and also crypt survival only in jejunum of mice pretreated with GBJJHB. Activities of SOD and catalase were significantly increased in GBJJHB treated group. These results suggest GBJGHB can be applied for prevention from side effects and damage by radiotherapy to cancer.
김동희,최주선,추지희,송호철,이은옥,강인철,최종원,김성훈 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6
Antitumor and antimetastatic effects of holyessing were studied. Cytotoxicity against various cancer cell lines, antiadhesion, Huvec cell proliferation, pulmonary colonization, and T/C% to S-180 and immunomodulatory effect were evaluated, respectively. The results were summarized as follows; HE exhibited significant cytotoxicity against Raw cell, B16-BL6 and Sk-Mel-2 cell in 1.0% conc. but didn't exhibit cytotoxicity against L929, HT-1080 and A549 cell. HE inhibited adhesion of HT1080 cell to complex extracellular matrix. And also inhibited the proliferation of HUVEC cell in dose-dependent manner. The T/C% was 113% in HE treated group in animal study with S-180. In pulmonary colonization assay by B16-F10, a number of colonies in the lungs were decreased but insignificantly. By FACS analysis, T cell and T-helper cell were significantly increased in HE treated group. From above results it was concluded that HE could be usefully applied for the prevention and treatment of cancer.
목초액과 항암성 본초가 가미된 복합목초액의 항암 및 항전이 활성에 미치는 영향
김동희,최주선,김성훈 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.1
Antitumor and antimetastatic effects of holyessing and complex holyessing supplemented antitumor herbs were studied. Cytotoxicity against various cancer cell lines, DNA topoisomerase I, pulmonary colonization, antiangiogenesis, and T/C% to S-180 were evaluated, respectively. The results were summarized as follows; Holyessing and Ganodema japonicum and Ginseng radix was not exhibited cytotoxicity against all cell lines, while and Oldenlandiae diffusae Herba showed a potent cytotoxic activities against B16-F10 cell lines. 20 times of diluted holyessing(1:20) and 150㎍/㎖ of Ganodema japonicum and Ginseng radix were shown significantly inhibited DNA topoisomerase I activity. The T/C% was 113% in holyessing(HSL) treated group respectively in animal study with S-180. In pulmonary colonization assay by B16-F10, a number of colonies in the lungs were decreased more significantly in complex holyessing(CHSL-A, CHSL-B) supplemented with Ganodema japonicum, Ginseng radix and Oldenlandiae diffusae Herba than in control group. In vitro neovascularization assays, angiogenesis was significantly inhibited in complex holyessing treated group(CHSL-A, CHSL-B) than in control group. Holyessing(HSL) and complex holyessing(CHSL-A, CHSL-B) groups strongly inhibited angiogenesis than control by CAM assay. Holyessing(HSL) induced aoptosis and and synergistically increased by complex holyessing(CHSL-A, CHSL-B) groups supplementing antitumor herbs. Key word : antitumor activity, holyessing, Complex holyessing, Ganodema japonicum, Ginseng radix, Oldenlandiae diffusae Herba, DNA topoisomerase I activity, pulmonary colonization, neovascularization, apoptosis