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DMBA 매식과 방사선 조사로 유도된 백서 타액선 종양에서 H-ras 암유전자의 활성화
허기수,최종환,최순철,박태원,유동수,Hu Key-Soon,Choi Jong-Whan,Choi Soon-Chul,Park Tae-Won,You Dong-Soo 대한영상치의학회 1998 Imaging Science in Dentistry Vol.28 No.1
Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutations has important prognostic implications in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA -implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the fOllowing results were concluded: 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/㎍lgDNA in the experimental group with the DMBA-implantation/irradiation according to results of transfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9% (5/36) in the experimental group with the DMBA implantation, 15.4 % (6/39) in the experimental group with the DMBA -implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6%(2/36) and 8.3%(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7%(3/39) in the experimental group with the DMBA -implantation/irradiation.
최승호(Seung-Ho Choi),최종환(Jong-Whan Choi),안계선(Kye-Sun Ahn) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-
본 논문에서는 VEX 시스템을 이용하여 자동 주행 장치를 구현하였다. 센서를 이용하여 장애물을 감 지하고, 각각의 바퀴를 조정하여 안전한 경로로 움직일 수 있는 자동주행 장치를 제작한 결과 만족한 주행 결과를 얻었다.
구재경,송승준,강선경(Sun-Kyung Kang),최종환(Jong-Whan Choi) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-
본 논문에서는 최근 소음으로 인해 인식률이 저하되는 현상을 보충하기 위해 사람의 입술 모양을 검출하여 소음과 같은 잡음환경에서 음성인식 기술을 함께 사용할 수 있는 알고리즘개발을 제안한다. 조명 변화에 견고한 인식결과를 얻기 위해 본 논문에서는 히스토그램 매칭과 입술 폴딩, RASTA 필터를 적용하였다. 또한 임베디드 환경에서 사용자의 입술모양을 실시간으로 동작하기 위해 입술검출 방법과 특징 추출 방법을 제안하였다. 본 논문에서 제안한 방법은 계산량이 작고 자원을 적게 사용함으로써 임베디드 환경에서 실시간으로 동작할 수 있었다.
AE를 이용한 마그네슘 합금 마찰용접부의 인장강도 예측
신창민(Chang-Min Shin),강대민(Dae-Min Kang),최종환(Jong-Whan Choi),곽재섭(Jae-Seob Kwak) 한국기계가공학회 2012 한국기계가공학회지 Vol.11 No.2
In this study, the friction welding experiment was performed by using the design of experiment. And the signal data acquired by acoustic emission sensor were analyzed to predict the tensile strength of friction welding part at friction welding process for AZ31 magnesium alloy. A dimensionless coefficient(ØAE), which consisted in the square of AE rms and variance, was defined as the characteristic of friction welding and the prediction equation was obtained by using linear regression. As the result of analysis, it was seen that the correlation between predicted and measured values became very close and on-line prediction of the ensile strength was possible in friction welding part.
실험계획법에 의한 AZ31마그네슘합금의 마찰접합시 최적공정설계
강대민(Dae-Min Kang),곽재섭(Jae-Seob Kwak),최종환(Jong-Whan Choi),박경도(Kyeong-Do Park) 한국기계가공학회 2011 한국기계가공학회지 Vol.10 No.4
Magnesium alloy has been known as lightweight material in automobile and electronic industry with aluminum alloy, titanium alloy and plastic material. Friction welding is useful to join different kinds of metals and nonferrous metals they are difficult to be joined by such as gas welding, resistance welding and electronic beam welding. In this study, friction welding was performed to investigate optimization process of Mg alloy with a 20㎜ diameter solid bar. For that, the orthogonal array (L9(34)) was used that contained four factors and each factor had three levels. Control factors were heating pressure, heating time, upsetting pressure and upsetting time. Also tensile tests were carried out to measure mechanical properties for welded conditions. The levels of heating pressure and upsetting pressure used were 15, 25, 35㎫, and 30, 50, 70㎫, respectively. In addition those of heating time and upsetting time were 0.5, 1, 1.5 sec and 3, 4, 5 sec., respectively, rotating speed of 2000rpm. From the experimental results, optimization condition was estimated as follows; heating pressure=35㎫, upsetting pressure=70㎫, heating time=1.5sec, upsetting time=3sec.