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        Pediococcus pentosaceus K1270에 의한 인공치태 형성억제 효과

        최외임,한수지,김신 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.4

        치태형성을 억제하는 유산균 K1270을 김치로부터 분리한 후 배양적, 생화학적 특징 및 16S rDNA 염기서열 분석에 의해 Pediococcus pentosaceus K1270으로 동정하였다. K1270 균주는 5% 자당이 함유된 배지에서 Streptococcus mutans Ingbritt에 의한 인공치태 형성을 대조군에 비해 94.6 % 억제하였으며, 비수용성 글루캔의 합성을 89.6 % 억제하였다. S mutans Ingbritt의 증식은 대조군에 비해 100배 정도 억제하였다. K1270균주는 TMB와 peroxidase가 첨가된 MRS 한천배치에서 과산화수소를 생산하였으며, 인공치태 형성에 대한 K1270균주의 억제 효과는 catalase첨가에 의해 일부 감소되었다. K1270 균주의 배양 상청액을 10 % 자당이 함유된 2×M17 broth에 동량 가한 경우 인공치태 형성 및 Streptococcus mutans Ingbritt의 증식이 억제되었으며, 이 억제효과는 catalase첨가에 의해 일부 감소되었고, 열처리, 또는 trypsin 처지에 의해 완전히 소실되었다. 따라서, 본 연구에서 분리, 동정된 P. pentosaceus K1270은 과산화 수소와 bacteriocin 유사물질을 분비하여 S. mutans Ingbritt의 증식을 억제함으로써 인공치태 형성을 억제하는 것으로 사료된다. Pediococcus pentosaceus K1270 was isolated from naturally fermented kimchi and identified based on the 16S rDNA sequence as well as cultural and biochemical characteristics. This strain strongly inhibited the formation of biofilm by Streptococcus mutans Ingbritt. K1270 also showed antibacterial activity against S. utans Ingbritt. It was observed that K1270 strain produced hydrogen peroxide on MRS agar supplemented with 3, 3, 5, 5-tetramethlybenzidine (TMB) and peroxidase and the inhibitory effect of K1270 strain on the biofilm formation was reversed by the addition of catalase in part. Culture supernatant of K1270 inhibited the biofilm formation and the multiplication of S. mtans Ingbritt. This inhibitory effect of culture supernatant was decreased slightly by the addition of catalase and abolished by heat or trypsin treatment. Thus, this study suggests that P. pentosaceus K1270 inhibit the biofilm formation through the inhibition of the replication of S. mutans Ingbritt by producing hydrogen peroxide and bacteriocin.

      • KCI등재

        S. mutans와 L. salivarius에 대한 glass ionomer 수복재의 항균성에 관한 연구

        김신,최외임 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.1

        For the purpose of evaluating the autibacterial antivities of some restorative materials to major cariogenic bacteria, the size and patterns of inhibition zone of amalgam, composite resin,glass ionomer cement, and glass cermet cement to Streptococcus mutans and Lactobacillus salivarius were assessed with diffusion-in gel method. The results were as follows: 1. To Streptococcus mutans was revealed strong antibacterial effect with glass cermet ce-ment, glass ionomer cement, amalgam in order. 2. The antibacterial activity to Lactobacillus salivarius was revealed with glass ionomer ce-ment and glass cermet cement. And the former was stronger. 3. Streptococcus mutans showed higher sensitivity than Lactobacillus salivarius in every material examined.

      • KCI등재
      • KCI등재

        어린이의 하악과두골절에 대한 비외과적 정복 및 악기능회복의 치험례

        이애련,김신,김주미,황보민,김동규,최외임 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

        The author treated a case of mandibular left condylar fracture in children with conservative measures utilizing elastic rubbers, and obtained the following results. 1. It will be better to adapt non-surgical conservative method based upon functional orthopedic approaches in condylar fractures of growing children, with the exception in the cases with bilateral fracture concomittent with displacement. 2. The treatment should be initiated soon after infliction of trauma and take more than 6 months of treatment-period. 3. In order to prevent the articular ankylosis, it would be better to minimize the period of intermaxillary fixation and allow the functional mandibular movement as soon as possible. In this case, a little more treatment for orthodontics and follow-up observation would be required.

      • KCI등재
      • KCI등재

        유전치부의 심미수복을 위한 Silicoated Resin-bonded stainless steel crown의 제작 및 치험례

        이애련,김신,최외임,황보민,김주미,김동규 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

        For the purpose of establishing the method of esthetic full coverage restoration with superior esthetics and strength for primary anterior teeth, a trial was made with silicoated resin-bonded stainless steel crown with enhanced bond strength between stainless steel and composite resin. The results were as follows: 1. The result was revealed superior in the aspects of esthetics, strength, color stability, labial thickness and chairtime which is very significant especially for un-cooperative children. 2. On the contrary, the necessity of more than 2 appointments and the aspect of economy due to the lack of common-use were remained to be solved from now on.

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