Gel filtration chromatography와 propionic acid/urea polyacrylamide gel electrophoresis를 이용한 봉독 성분의 분리

최영권,최석호,권기록,Choi, Young-Chon,Choi, Suk-Ho,Kwon, Ki-Rok 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2

Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase $A_2$ and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

결핵성 흉막염 환자에서 흉수 내 Matrix Metalloproteinases 및 Tissue Inhibitors of Metalloproteinases 농도와 잔여 흉막비후와의 관계

최영권 ( Young Kwon Choi ),안창혁 ( Chang Hyeok An ),김유진 ( Yu Jin Kim ),경선영 ( Sun Young Kyung ),이상표 ( Sang Pyo Lee ),박정웅 ( Jeong Woong Park ),정성환 ( Sung Hwan Jeong ) 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.65 No.1

연구배경: 잔여 흉막비후는 결핵성 흉막염 치료 후 흔히 나타날 수 있는 합병증 중의 하나이며, 이로 인해 호흡 기능에 지장을 주는 경우가 있다. 이에 결핵성 흉막염 진단 시 흉수 내의 metalloproteinase (MMP)s와 tissue inhibitor of metalloproteinase (TIMP)s의 농도가 치료 후 잔여 흉막비후가 지속되는 지 예측할 수 있는 인자가 될수 있는 지 알아보고자 하였다. 방법: 2004년 1월부터 2005년 6월 사이에 흉수가 발견되어 입원한 환자를 대상으로 전향적 연구를 시행하였다. 진단 시 흉수의 분석을 통해 결핵성 흉막염, 부폐렴성 흉수, 악성 흉수, 여출액군으로 나누고, 환자의 혈청과 흉수에서 ELISA 방법을 이용하여 MMP-1, -2, -8, -9와 TIMP-1, -2를 측정하였다. 결핵성 흉막염의 경우 흉부엑스선검사로 항결핵제 치료 종결 시점과 마지막 추적 관찰시점에 잔여 흉막비후의 두께를 측정하여 잔여 흉막비후가 있는 군과 없는 군으로 나누었다. 결과: 흉수가 발견되어 입원한 환자 중 제외 기준에 해당하는 환자를 제외하고 총 39명의 환자가 대상이 되었다. 이 중 결핵성 흉막염은 23명, 부폐렴성 흉수 7명, 악성 흉수 7명, 여출액 2명이었다. 결핵성 흉막염 환자 23명중 본원에서 항결핵제 치료를 종료한 환자는 17명이었으며, 이 중 잔여 흉막비후가 없는 군은 10명(59%)이었으며, 잔여 흉막비후가 있는 군은 7명(41%)이었다. 잔여 흉막비후가 있는 군은 흉수 TIMP-1 (41,405.9±9,737.3 ng/mL)이 잔여 흉막비후가 없는 군(29,134.9±8,801.8)보다 의미있게 높았다(p=0.032). 치료 종료 후 평균 8±5개월의 추적관찰이 가능한 13명의 환자들에서, 마지막으로 촬영한 흉부 후전위 촬영에서 잔여 흉막비후가 없는 군은 11명(85%)이었고, 잔여 흉막비후가 있는 군은 2명(15%)이었다. 잔여 흉막비후가 있는 군은 흉수 TIMP-2 (34.4±6.5ng/mL)가 잔여 흉막비후가 없는 군(44.4±15.5)보다 의미있게 낮았다(p=0.038). 결론: 결핵성 흉막염의 잔여 흉막비후의 발생에 TIMP-1과 TIMP-2이 관여 될 수도 있을 것으로 추정된다. Background: Residual pleural thickening (RPT) is the most frequent complication of tuberculous pleurisy (TP), and this can happen despite of administering adequate anti-tuberculous (TB) therapy. Yet there was no definite relation between RPT and other variables. The aim of this study was to examine matrix metalloproteinases (MMPs) and the inhibitors of metalloproteinases (TIMPs) and to identify the factors that can predict the occurrence of RPT. Methods: The patients with newly-detected pleural effusions were prospectively enrolled in this study from January 2004 to June 2005. The levels of MMP-1, -2, -8 and -9, and TIMP-1 and -2 were determined in the serum and pleural fluid by ELISA. The residual pleural thickness was measured at the completion of treatment and at the point of the final follow-up with the chest X-ray films. Results: The study included 39 patients with pleural fluid (PF). Twenty-three had tuberculous effusion, 7 had parapneumonic effusion, 7 had malignant effusion and 2 had transudates. For the 17 patients who completed the anti-TB treatment among the 23 patients with TP, 7 (41%) had RPT and 10 (59%) did not. The level of PF TIMP-1 in the patients with RPT (41,405.9±9,737.3 ng/mL) was significantly higher than that of those patients without RPT (29,134.9±8,801.8) at the completion of treatment (p=0.032). In 13 patients who were followed-up until a mean of 8±5 months after treatment, 2 (15%) had RPT and 11 (85%) did not. The level of PF TIMP-2 in the patients with RPT (34.4±6.5 ng/mL) was lower than that of those patients without RPT (44.4±15.5) at the point of the final follow-up (p=0.038). Conclusion: The residual pleural thickening in TP might be related to the TIMP-1 and TIMP-2 levels in the pleural fluid.

봉약침의 LD₅₀ 변화 보고

장성봉,최영권,권기록,Jang, Seong-Bong,Choi, Young-Kwon,Kwon, Ki-Rok 대한약침학회 2005 Journal of pharmacopuncture Vol.8 No.1

Objectives : This experiment was conducted to reevaluate $LD_{50}$ of Korean bee venom acupuncture as many changes have occurred over the years. Methods : ICR mice were used as the experiment animals and bee venom acupuncture was manufactured under the protocols of Korean Institute of herbal Acupuncture. Based on the previous reports, experiment was divided into pre and main sections. Results : 1. Presumed $LD_{50}$ value is at 5.25mg/kg 2. Deaths of experiment animals occurred within 48 hours. 3. Reduced toxicity of the bee venom acupuncture is likely to be the results of more refined manufacturing process and production. Conclusion : Comparing with the values of the previous results, toxicity of the bee venom acupuncture showed significant changes and more accurate findings on $LD_{50}$ value must be accomplished to lead further studies on the bee venom acupuncture.

급성 신우신염이 병발한 급성 연쇄상구균 감염후 사구체신염 1례

조창이,최승희,최영권,김병희,유용상,유용상,김준성,Cho Chang-Yee,Cho Seung-Hee,Choi Young-Kwon,Kim Byung-Hee,Yoo Yong-Sang,Yoo Yong-Sang,Kim Joon-Sung 대한소아신장학회 2004 Childhood kidney diseases Vol.8 No.2

저자들은 두통, 안면 부종, 고열 및 옆구리 통증 등을 주소로 내원한 5세 여아에서 특징적인임상 양상과 검사 소견 등을 통하여 첫 발병시에 E. coli 감염에 의한 급성 신우신염이 병발한 급성 연쇄상구균 감염후 사구체신염으로 진단하고 치료한 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Acute poststreptococcal glomerulonephritis(APSGN) is the most common form of postinfectious glomerulonephritis, and acute pyelonephritis(APN) is the most severe form of urinary tract infection in childhood. However, the concurrence of two diseases is uncommon in the literature. We describe a case of APSGN accompanied with APN in a 5-year-old female who presented with fever, left flank pain, headache and facial edema. Urinalysis showed pyuria, microscopic hematuria, and mild proteinulra. Serial urine cultures grew Escherichia coli. ${^99m}$Tc-DMSA renal scan revealed a cortical defect in the upper pole of left kidney. She had a history of preceding pharyngitis, in addition, showed high blood pressure, high anti-streptolysin 0 titer, and low serum complement levels. The patient improved completely with supportive treatment, Including antibiotic and antihypertensive therapy. These findings suggested that APSGN and APN could be manifested simultaneously or be .superimposed on each other.

플랜트 민감설비용 가스계 소화시스템을 위한 저소음 노즐의 성능 평가

김준희(Jun Hee Kim),최영권(Young Kwon Choi),이홍철(Hon Cheol Lee),노고섭(Go Seop Rho),박종원(Jong Won Park),황인주(In Ju Hwang) 대한설비공학회 2020 대한설비공학회 학술발표대회논문집 Vol.2020 No.6

본 연구에서는 오일·가스 플랜트 민감설비에 적용되는 소화시스템의 약제 방출소음 저감을 위해 새롭게 설계된 소화 노즐을 제시하고 이를 기존 상용 소화노즐과의 비교 실험을 통해 소음저감 수준을 평가하였으며 흡읍재의 증가와 흐름우회유로의 유무에 따른 소음 저감 정도를 분석하였다. 총 6가지의 시험 케이스가 고려되었으며 소음 측정 실험을 위하여 IG-100 소화제를 이용한 실험 설비가 구성되었다. 새롭게 고안된 저소음 노즐은 기존 상용 노즐과 해외 저소음 노즐 대비 각각 약 23.7%와 3.6% 감소된 소음치를 나타내었다. 흡음재는 소음저감에 큰 영향을 미치지만 일정 수준 이상에서는 그 효과가 미비한 것으로 나타났으며, 흐름우회유로 또한 소음저감에 상당한 효과를 나타내는 것을 확인하였다.

증례 : 소화기; 보존적 치료로 호전된 장괴사를 동반한 급성 하장간막 동맥 혈전증 1예

최윤식 ( Yun Sik Choi ),양동훈 ( Dong Hoon Yang ),남광우 ( Kwang Woo Nam ),이신원 ( Sin Won Lee ),최영권 ( Young Kwon Choi ),변정식 ( Jeong Sik Byeon ),양석균 ( Suk Kyun Yang ) 대한내과학회 2013 대한내과학회지 Vol.85 No.6

급성 장간막 혈전증은 대부분 상장간막 동맥에 발생하므로 그 예후가 매우 불량하며 특히 장괴사를 동반한 경우에는 반드시 수술이 필요하다. 그러나 저자들은 비록 장괴사가 동반되었으나 하장간막 동맥에 부분적인 폐색을 유발한 혈전증을 보존적 치료만으로 성공적으로 치료하였기에 문헌고찰과 함께 보고하는 바이다. Acute mesenteric thrombosis accounts for 25-30% of acute mesenteric ischemia and occurs usually alongside severe atherosclerotic disease. Acute mesenteric thrombosis primarily affects the superior mesenteric artery; thus, inferior mesenteric arterial thrombosis is an extremely rare form of the condition. Surgical treatment is mandatory to resolve impending or overt bowel infarction in acute mesenteric ischemia patients. However, here we report a case of colonic infarction caused by acute inferior mesenteric thrombosis successfully treated by conservative management. (Korean J Med 2013;85:604-608)

담도암 진단을 위해 담도배액관에서 얻은 담즙세포진검사의 유용성에 관한 연구

김진용 ( Jin Yong Kim ),최준혁 ( Joon Hyuk Choi ),김진희 ( Jin Hee Kim ),김창래 ( Chang Lae Kim ),배승현 ( Seung Hyeon Bae ),최영권 ( Young Kwon Choi ),하연정 ( Yeon Jung Ha ),송민주 ( Min Joo Song ),최준호 ( Jun Ho Choi ),홍승모 대한소화기학회 2014 대한소화기학회지 Vol.63 No.2

Background/Aims: Biliary drainage is performed in many patients with cholangiocarcinoma (CCA) to relieve obstructive jaundice. For those who have undergone biliary drainage, bile cytology can be easily performed since the access is already achieved. This study aims to determine the clinical usefulness of bile cytology for the diagnosis of CCA and to evaluate factors affecting its diagnostic yield. Methods: A total of 766 consecutive patients with CCA underwent bile cytology via endoscopic nasobiliary drainage or percutaneous transhepatic biliary drainage from January 2000 to June 2012. Data were collected by retrospectively reviewing the medical records. We evaluated the diagnostic yield of bile cytology with/without other sampling methods including brush cytology and endobiliary forcep biopsy, and the optimal number of repeated bile sampling. Several factors affecting diagnostic yield were then analyzed. Results: The sensitivity of bile cytology, endobiliary forceps biopsy, and a combination of both sampling methods were 24.7% (189/766), 74.4% (259/348), and 77.9% (271/348), respectively. The cumulative positive rate of bile sampling increased from 40.7% (77/189) at first sampling to 93.1% (176/189) at third sampling. On multivariate analysis, factors associated with positive bile cytology were perihilar tumor location, intraductal growing tumor type, tumor extent ≥20 mm, poorly differentiated grade tumor, and three or more samplings. Conclusions: Although bile cytology itself has a low sensitivity in diagnosing CCA, it has an additive role when combined with endobiliary forceps biopsy. Due to the relative ease and low cost, bile cytology can be considered a reasonable complementary diagnostic tool for diagnosing CCA. (Korean J Gastroenterol 2014;63:107-113)

증례 : 소화기; 내시경 유두절제술로 제거한 거대 십이지장 주유두의 신경내분비종양

배승현 ( Seung Hyeon Bae ),김진용 ( Jin Yong Kim ),김창래 ( Chang Lae Kim ),최영권 ( Young Kwon Choi ),신보미 ( Bo Mi Shin ),홍승모 ( Seung Mo Hong ),김명환 ( Myung Hwan Kim ) 대한내과학회 2014 대한내과학회지 Vol.86 No.3

Neuroendocrine tumors (NET) of the major duodenal papilla are rare and the natural history of this disease is not clear. We experienced a case in a 31-year-old male. Duodenoscopy revealed an enlarged major duodenal papilla with central umbilication and nodularity. Endoscopic ultrasonography (EUS) demonstrated a 3-cm hypoechoic mass that was confined to the submucosa. A biopsy led to the diagnosis of a grade 1 NET. The patient refused surgery, so we performed an endoscopic papillectomy. The tumor was removed completely. The resected specimen confirmed the diagnosis of a well-differentiated NET and all resection margins were negative. Surgical resection is currently considered to be the gold standard for the treatment of a large NET of the major duodenal papilla; however, endoscopic resection is a possible treatment modality for patients at high surgical risk or who are reluctant to undergo surgery.

증례 : 신장 ; 신 이식 후 항체 매개성 거부반응 환자에서 지연성 회복을 보인 치료 1예

조형진 ( Hyung Jin Cho ),신은혜 ( Eun Hye Shin ),양영주 ( Young Joo Yang ),정지원 ( Ji Won Jung ),지원준 ( Won Jun Ji ),최영권 ( Young Kwon Choi ),박수길 ( Su Kil Park ) 대한내과학회 2014 대한내과학회지 Vol.86 No.2

항체 매개성 거부반응은 이식 신 기능상실에 중요한 원인으로 현재까지 확립된 치료방법이 없는 상태이다. 항체 매개 성 거부반응의 원인으로 생각되는 제공자 특이 항체의 경우 면역억제제를 잘 복용하지 않아서 지연성으로 발생하는 경우가 있어, 지속적인 면역억제제 복용의 교육이 필요하다. 항체 매개성 거부반응에 있어 최근 치료로 도입된 bortezomib 단독치료로서 회복을 보이지 않았던 증례에서 rituximab을 추가로 치료하여 지연성 회복을 보인 증례를 보고하며 일반적인 치료법으로도 회복되지 않는 항체 매개성 거부반응에서 rituximab과 bortezomib의 병합치료를 고려해 볼 수 있겠고 추가적인 연구가 필요할 것으로 생각된다. Antibody-mediated rejection (ABMR) in kidney transplant recipients is mediated by donor-specific antibodies. It is the major cause of graft failure in noncompliant patients and is associated with reduced long-term graft survival. We present a case of delayed recovery of renal function despite aggressive therapy after acute ABMR. A 49-year-old male on triple-drug maintenance immunosuppression (prednisolone, cyclosporine, and azathioprine), who underwent cadaveric donor renal transplantation 14 years earlier, visited our clinic with a serum creatinine level (SCr) of 1.9 mg/dL. The kidney biopsy revealed acute ABMR with diffuse C4d immunopositivity. We started steroid pulse therapy and bortezomib with plasmapheresis. Nevertheless, the SCr increased. Consequently, antithymocyte globulin (ATG) and intravenous immunoglobulin were administered. The SCr increased further to 4.1 mg/dL. Therefore, we performed a second kidney biopsy, which showed no change. Finally, we used rituximab. Fortunately, the SCr decreased gradually and returned to baseline. (Korean J Med 2014;86:237-241)

인간과 로봇간의 움직임의 무선 인터페이스 구현

전풍우(Poong-Woo Jeon),조현택(Hyun-Taek Cho),장평수(Pyoung-Su Jang),조기호(Ki-Ho Cho),김정구(Jeong-Ku Kim),송덕희(Duk-Hee Song),최영권(Young-Kwon Choi),정슬(Seul Jung) 제어로봇시스템학회 2001 제어로봇시스템학회 국제학술대회 논문집 Vol.2001 No.10