稙物種子油의 Triglyceride 組成에 關한 硏究

崔守安 부산교육대학교 과학교육연구소 1986 科學敎育硏究 Vol.11 No.-

The triglyceride compositions of peach kernel and apricot kernel oil have been investigated by a combination of high performance liquid chromatography (HPLC) and gas liquid chromatography (GLC). The triglycerides of peach kernel and apricot kernel oil were first separated by thin layer chromatography (TLC), and fractionated on the basis of their partition nunber (PN) by HPLC on a C-18_μ-Bondapak column with methenol-chloroform solvent mixture. Each of acyl carbon number (CN) of triglyceride. Also the fatty acid compositions of these triglycerides were determinded by GLC. From the consecutive analyses of these three chromatography techniques, the possible triglyceride compositions of peach kernel and apricot kernel oil were combinated into fifteen and thirteen kinds of triglycerides, respectively. The major Triglycerides of peach kernerl oil were those of (3×C_(18:1), 30.9%), (2×C_(18:1), C_(18:2), 21.2%), (C_(18:1), 2×C_(18:2), 10.6%), (3×C_(18:2), 3.8%), (C_(18:0), 2×C_(18:1), 1.8%), (C_(16:0), C_(18:1), C_(18:2), 1.5%), (C_(18:0), C_(18:1), C_(18:2), 1.1%), and those of apricot kernel oil were(3×C_(18:1), 39.5%), (2×C_(18:1), C_(18:2), 24.5%), (C_(18:1), 2×C_(18:2), 14.2%), (3×C_(18:2), 2.0%).

Malonaldehyde 및 Hexanal의 DNA 損傷作用

崔守安 부산교육대학교 과학교육연구소 1990 科學敎育硏究 Vol.15 No.-

The damage of plasmid DNA by malonaldehyde and hexanal to elucidate the DNA damage mechanism by lipid peroxidation products was investigated through 1% agarose gel electrophoresis. DNA damage was induced by malonaldehyde and dexanal, byt its mechanism was due to the formation of the DNA-carbonyl compound complex, not to the scission of DNA strand. And there was not the effects of avtive oxygens on the DNA damage by malonaldehtde and hexanal.

Linoleic acid의 酸化에 의한 活性酸素種의 生成

최수안 부산교육대학교 과학교육연구소 1989 科學敎育硏究 Vol.14 No.-

The Formation of superoxide anion(·O_2^-)and hydrogen peroxide(H_2O_2) during linoleic acid peroxidation were investigated in linoleic acid-aqueous system at 37℃. Superoxide anion was rapidly generated in the early stage of peroxidation, marked to 0.375(absorbance at 560mm) in the 12mM linoleic acid(POV below 80millieq/kg) incubated for Ⅰ day and then decrased with time-elapsed. Hydrogen peroxide was also rapidly generated in the early stage of peroxidation regardless of linoleic acid concentration. And, superoxide dismutase (SOD) and catalase greatly inhibited the formation of superoxide anion and hydrogen peroxide, respectively.

간수 중의 各成分組成에 對하여

崔守安 釜山敎育大學 1968 부산교육대학 논문집 Vol.4 No.1

This study aims to make clear the interrelations between each ion contained in salt produced by means of spontaneous evaporation and in salt produced by means of boiling evaporation. Comparative examination between each composition in the above mentioned kinds of salt was attemptted and the composition analysis of brine was conducted under the following methods. 1. The quantity of Ca was determined through the method of volumetric analysis that positive ion in the sample was extracted by precipitating with H_2S and ammonia liquid, the filtered liquid was alkalized and was precipitated into CaC_2O_4 with (NH_4)_2C_2O_4 sol, the precipitated material was decomposed into C_2O_4H_2 sol with dil H_2SO_4 and finally this C_2O_4H_2 sol was titrated with KMnO_4 sol. 2. The quantity of Mg was determined by adding (NH_4)_2HPO_4 sol to the liquid of Mg salt, by precipitating this with MgNH_4PO_4 to burn, and finally by determining this as Mg_2P_2O. 3. K was quantitatively analyzed by melting the compound of NaCl, Kcl into the precipitation of KClO_4 with HClO_4 so. 4. The compound of Nacl, Kcl was precipitated with HClO_4 sol into KClo_4 and the value of K was taken from Nacl, Kcl to get the value of Na. 5. The quantitative determination of Cl followed the Mr. Mohr's method. 6. The quantity of Br_2 was determined by adding K_2Cr_2O_7, H_2SO_4 to the sample to boil and by collecting the separated Br_2 from the compound liquid of NaOH, H_2O_2 sol. this collected NaBr liquid was again added to by K_2Cr_2O_7, H_2SO_4 and boiled in order to collect this separated Br_2 into Kl sol. This separated iodide was titrated with normal solution of Na_2S_2O_3 sol to get the quantity of Br_2 7. The quantity of SO_4, was determined by the method that the sample was slightly acidified with Hcl and to this solution BaCl_2 sol was added and precipitated with BaSO_4 and then dried by heating in order to get the determination. The result of this analysis shows the fact that generally the amount of each composition of salt made by means of boiling evaporation is greater than that of salt made by means of spontaneous evaporation except the fact that the amount of Cl' is almost same in both cases. The sequence of the amount of each composition in salt is as follows: Cl″, SO″_4, Mg, Na, K, Br_2, Ca(from the highest amount to the lowest).

Micro Thin Layer Chromatography에 의한 식품 중의 색소 및 Amino Acid의 분리

최수안 釜山敎育大學 1970 부산교육대학 논문집 Vol.6 No.1

The writer has tried in this study to separate pigments and amino acfds from the various Kinds of food product by means of the Thin Layer Chromatography in order to get some physiological reactions on human bodies of noxious Pigments, find out the nutritive value of various amino acids, and consequently to add to the improvement of the national health service, 1. Separation of Pigment First the various Kinds of pigment contained in liquid juice, sheep's milk, cow's milk, soda water, candy, canned berry, canned pineapple, et c. have been separated; and the frequency of use of these pigments have been examined. and then the unauthorized pigments have been differentiated from the authorized ones, The pigments contained in these food products are as follows: Tantrazine, Amaranth, Sunset yellows FCF, Erythrothine, Brilliant Blue FCF, Griner Green B, Light Green SF yellowish, Fast Green FCF, Acid Violet 6B, Indigo Carmine, Quramine, Rhodamine, yellow OB, Ponso 3R, Yellow AB, Ponso R, 2. Separation of Amino Aeid In order to separate the various Kinds of amino acid contained in soy sauce, grape wine, strawberry wine, which are home made, and tangle(sea weed), protein has been hydrolyzed following the Fither's Organic chemical Experiment Method, and from this hydrolyzed liquid, the varions Kinds of amino acid have been separated, The development of the work has followed the first dimensional method and the second dimensional method, (1) In soy sauce, Asparatic Acid, Lysine, Glutamic Acid, Arginine, Serine, Glycine, Threonine, Valine, Leucine or lst Leucine, Phenyl Alanine, are contained. (2) In0 grape wine, Lysine, Cystine, Threonine or Tyrosine, valine, Leucine or lso Leucine, Tryphtophan or Methionine or phenyl Alanine, are contained. (3) In strawberry wine, Lysine, Cystine, Threonine or Tryosine, Valine, lso Leucine or Leucine, Tryphtophan or Methionine or Phenyl Alanineyc, are contained. (4) In tangle(sea weed), Lysine, Cystine, Threonine or Tryosine, valine, lso Leusin or Leucine, Tryphtophan or Methionine or phenyl Alanine, are contained.

금속판막교체술 후 초기 6개월간 Warfarin 유지용량 변화와 이에 영향을 미치는 요인 분석

최수안,정영미,정소영,한현주,이병구,박경호,조남춘 한국병원약사회 2000 병원약사회지 Vol.17 No.2

Patients with mechanical heart valve prostheses require lifelong anticoagulation to reduce the risks of valve thrombosis and thromboembolism. These risks are greatest in the first three months after surgery and are exacerbated by inadequate anticoagulation. Although it is recognised that anticoagulant control is worse in the first few months after starting treatment, the relationship of this to warfarin dosing and the frequency of monitoring is unclear. We have therefore assessed the precision of anticoagulant control in the first three months after mechanical heart valve replacement and taken and analysis of influencing factors. Eighty-five consecutive patients who underwent isolated mechanical heart valve replacement between April 1997 and March 1998, were identified retrospectively from hospital records. Of these were excluded patients in taking a medicine appearing drug interactions or decreasing the liver function. The clinical data included patients demographics, warfain dosages and INR changes for the first six months after surgery, weight changes, warfain maintenance dosages by age, sex, valve type. Warfarin mean daily doses were 3.79±1.9, 3.60±1.67, 4.04±1.5, 4.36±1.6, 4.62±1.42, 4.98±1.6㎎ at postdischarge, 2 weeks, 1, 2, 3 and 6 months, respectively. Theses increased significantly. Patients gained weight but it was not significantly between weight and warfain dosages changes. Theses were not influenced by the valve type, age but significantly increased 19.3±0.31% male, 80±0.84% female, respectively. These results suggested that there is a sex difference in the anticoagulation effects of warfarin. The anticoagulant response to warfarin is changed for the first three month after mechanical valve replacement. Therefore it is nescessary to control strictly for this periods.

自然科 實驗學習指導方法의 硏究 : 탐구학습의 효율화를 위한 재료개발

최수안,오진태,옥치율 부산교육대학교 과학교육연구소 1985 科學敎育硏究 Vol.10 No.-

It may be said that the mainpost of natural science study is the inquring study and as for the point of observation and experiment which is a kind of inquiring process, the success of natural science study is dependent upon study materials. Therefore, in order to develop more effective inquring activities, successive studies such as the ploblem solution, improvement and development of study materials are needed. Several units is the field of chemistry and biology are selected, and several study materials improved and developed are examined as followers: 1. Several experiment methods for the game in order to observe the presence of air are tried. 2. In order to observe the solution phenomena of air easily, new experiment designe is devised, ammonia is soluble in the water including two drops of phenolphthalein and the solution phenomena of air changing into red. 3. In the experiment of the generation of carbon dioxide using every kind of shelfish instead of lime, the amount of the generation of carbon dioxide from the concentration of hydrochloric acid and the amount of shelfish is compared and the effort of substitute materials is examined. 4. The utility factor or the inquring study is devised through the utilization of waste materials, the development of substitute materials and the productive activities of instrument observation using study materials used separated units of materials. 5. It is found that a tadpole, a mud-snail and a killifish substitute for a planaria with a living thing material in the field of biology is available for the revival experiment.

自然科 學習資料의 開發 : "물질의 分離" 單元을 中心으로 An Improvemnet Plan in Unit "Separation of Matter", "Environment and plant"

崔守安,金世榮 부산교육대학교 과학교육연구소 1977 科學敎育硏究 Vol.2 No.-

Linoleic acid의 酸化에 의한 DNA 損傷作用 및 活性酸素種의 역할

崔守安,姜珍壎 부산교육대학교 과학교육연구소 1987 科學敎育硏究 Vol.12 No.-

The present study was attempted to investigate on the damage of plasmid DNA by lipid peroxidation and the effects of the active oxygen radicals through the reaction of DNA and linileic acid at 37℃ as model systems. The dgree of DNA damage indeced by linoleic acid peroxidation was increased with increasing of linoleic acid content in reaction system. Linoleic acid at concentration above 10 mM induced the nonspecific double strands scission during peroxidation for 3 days, but the POV was below 100 millieq./kg at that time. And, generally, all of the active oxygen scavengers inhibited the DNA damage and the increase of POV by linoleic acid peroxidation, and such effects were greater in singlet oxygen scavengers and superoxide anion scavengers, but not shown in hydroxyl radical scavengers and hydrogen peroxide scavenger after 1 day incubation. From these profiles, it is concluded that singlet oxygen and superoxide anion geatly affected to the DNA damage induced by linoleic acid peroxidation, but hydrogen peroxide and hydroxy 1 redical participated in the early stage of peroxidation.

植物油의 脂肪酸組成에 關한 硏究 : 枸杞子의 脂肪酸 組成

최수안 부산교육대학교 과학교육연구소 1988 科學敎育硏究 Vol.13 No.-

Fatty acid compositions were incestigated from Licium chinesemil oil. The oil classes, polar and nin polar of the fractions, and each of oil fractions was identified by TLC on the Desitometer. The fatty acids of oil for ech kind were analyzed by GLC. These results, are as fellows: Licium chinesemill oil were found to be PE, 38.5%; PS, 37.61%; PC, 20.71%; SPM, 3.11%; in polar oil, TG, 90.75%; SE, 4.95%; FFA, 2.44%; ST, 2.04%; in non polar oil nd the major fatty acid in oil were identified to be C18;2, 65.8%; c18:1, 14.69%; and c16:0, 12:00%;.