백일해 예방약의 백혈구 증가 인자에 관한 연구

최성배 대한미생물학회 1985 大韓微生物學會誌 Vol.20 No.1

It was well known that B. pertussis cells possess protective antigen, histamine sensitizing factor, he- atstable and labile toxin, hemagglutinin, agglutinogen and the others. Previous reports involving above antigenic properties of B. pertussis have been carried out fnr several years. However, leucocyte promo- ting property was not yet investigated. In this report, the resu1ts of studies on the leucocytosis, particulary the lymphocytosis, produced in mice by injecting pertussis vaccine were presented. Especially leueocyte promoting property and histamine sensitizing property of B. pertussis vaccine treated at various temperatures were compared. The relationship between the leucocyte prornoting property and histamine sensitizing property was investigated. Results were as follows; 1. Although leucocytosis was significantly rised in both 0.5ml injection and D.lml injection of pertus- sis vaccine than in control, at the higher dose (0.5ml injection) an elevation in white cell count was more significant. The leucocyte response to pertussis vaccine was greater following 0.5 ml injection than following O.lml injection. 2. Lymphocytosis was significantly rised in both 0.5ml injection and O.lm! Injection of pertussis vac- cine than in control. At higher dose (0.5ml injection), an elevation in lymphocyte count was more significanl. 3. Order of elevation in differential leucocyte counts was lymphocyte, polymorphonuclear leucocyte and monocyte. 4. The leucocyte response to pertussis vaccine was 2 fold greater following intravenous injection than follawing subcutaneous injection. 5. Decrease leucocyte promoting activity and histarnine sensitizing activity resulted from exposure to temperature above 56C. Histamine sensitizing activity of pertussis vaccine treated at various temperatures paralleled leucocyte promoting activity.

Adenovirus의 접종량 변화에 따르는 증식 곡선과 세포 병변

최성배 대한바이러스학회 1989 Journal of Bacteriology and Virology Vol.19 No.2

Adenovirus 증식에 미치는 Hormone의 영향

최성배 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.2

Approximately 40 separate adenoviruses have been isolated from man, Viruses in this group replicate in the nucle of infected cells and are of similar size (70-90 nm in diameter) and shape. All members appear to have a core cornposed of single molecule of DNA and common complement fixing structure virus antigen. Adenovirus infection in man produce acute pharygitis, tracheo bronchitis, bronchopneumonia or conjunctivitis. Several members of this group, including types commonly associated with respiratory disease in man, are capable of producing rnalignant tumors in young hamsters, and a few types have been shown to be oncogenic in young rats. Adenoviruses types 1, 2 and 5 have been recovered from surgically removed tonsil and adenoids and have been also associated with sporadic mild respiratory illness of infant and children. To obtain evidence concerning the effect of hormone on adenoviruses replication, investigation of 4 proto type viruses of this group was under taken. For practical consideration it was desirable to investigate of the effect of hormone (estradiol- 17B) on adenoviruses yields in L cells. Results were as follows: 1. Yields of adenovirus type 1, 4, 6, in L cell were inhibited by the treatment of estradiol-17B. 2. No significant effect of estradiol-17B was noted in productive system of adenovirus type 2.

Adenovirus증식의 초기과정

최성배 대한바이러스학회 1988 Journal of Bacteriology and Virology Vol.18 No.2

Approximately 30separate adenovisuses have been isolated from man. Visuses in this group replicate in the nuclei of infected cells and are of similar size(60-90 nm in diameter) and shape. All mernbers appear to have a core composed of single molecule of DNA and common compliment fixing structure visus antigen. Adenovirsus infection in man produce acute pharygitis, tracheo bronchitis, bronchopneumonia or conjunctivitis. Several members of this group, including types commonly associated with respiratory disease in man, are capable of producing malignant tumors in young hamsters, and a few types have been shown to be oncogenic in young rats. Adenoviruses types 1, 2 and 5 have been recovered from surgically removed tonsil and adenoids and have been also associated with sporadic mild respiratory illness of infant and children. To obtain evidence concerning the early period of multiplication of adenoviruses, investigation of 4 proto type viruses of this group was undertaken. For practical consideration it was desirable to investigate of the adsorption and initial cycle of multiplication. The experimental results indicate that the adenoviruses studied adsorb at a relatively slow rate to Hela cell; that the latent or eclipse period of the multiplication cycle is relatively long and is longer for type 1 and 2 than for type 4 and 5; that the incremental period of viral propagation is relatively short. Results were as follows : 1. Following combination of virus with Hela cell(direct measurement of adenovirus adsorption), maximun virus adsorption required 6-9 hours. 2. Result of development of cytopathic effects in cells exposed to virus for varing periods (indirect measurement of adenovirus adsorption) indicate that adenovirus type 1 and type 5 were adsorbed faster than type 2 and type 4. 3. The latent or eclipse period of the multiplication cycle is relatively long and is longer for type 1 and 2 than for type 4 and 5. The latent period for type 1 and 2 viruses were approximately 18 hours, whereas for the related type 4 and 5 viruses the latent periods were significantly shorter, lasting about 12-13 hours. 4. The incrernental period of viral propagation is relatively short. Incremental period for type 1 and 2 viruses was 10-12 hours. For the type 4 and 5 viruses, howver, incremental period was somewhat longer.

Adenovirus와 항원 생성에 관한 연구

최성배 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Adenoviruses possess complement-fixing antigen which has been separated from infectious virus. It is a subject to determine whether the CF antigen represents a precursor of infectious adenovirus particle or whether the CF antigen is a product formed independently and concomitantly with infectious particle. This report describes the experiments which study the relationship between CF antigen and infectious virus and determine the time of detection of these two properties. In these experiments adenovirus types 1, 4, 5, were employed. Results were as follows. 1. Intracellular CF antigen had eclipse period of 18-21 hrs and intracellular infectious was detected 16-21 hrs after infection of HeLa cells. 2. CF antigen and infectious virus were produced independently and the CF antigen was not a precursor to infectious virus. 3. HeLa cells infected with adenovirus type 4 were capable of continued antigen production despite early development of maximun cytopathogenic effect. 4. The present study with adenovirus types, 1, 4, 5 indicated these viruses have similar growth cha- racteristics.

온도와 pH변환에 따르는 Adenovirus의 안전성

최성배 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.2

아메리카 사실주의와 표면사이

崔聖培 전주대학교 예술문화연구소 1997 예술문화 Vol.2 No.-

대구가톨릭대학교 자동차부품 지역혁신센터(RIC) 현황 및 전망

최성배,김기대 한국공작기계학회 2007 한국공작기계학회지 Vol.16 No.1

장티프스균의 항원성에 관한 연구

최성배 대한감염학회 1984 감염 Vol.16 No.2

The antigenic structure of Salmonella typhi has been reported by many investigators. However, the location in the cell of active material is not known with certainty. Thus, the present experiment was carried out to determine the antigenic structure of Salmonella typhi. In this study, the method of the preparation of cell wall, protoplasm and whole cell of Salmonella typhi was described and protective antigenicity and toxicity of these preparations of Salmonella typhi were presented. Results were as follows: 1) The protective antigen of Salmonella typhi investigated by mouse protection test was mainly located in cell wall preparation. The order of protective antigen of the preparations of Salmonella typhi was cell wall, whole cell and protoplasm. 2) The toxicity of Salmonella typhi was mainly present in the cell wall preparation. The order of toxicity of the preparation was cell wall, whole cell and protoplasm.

寫眞의 被寫體와 背景의 露出測定 比較

崔聖培 Natural Science Research Institute JEONJU UNIVERSI 1986 전주대학교 자연과학연구소 학술논문집 Vol.1 No.1

The relation between photo Subject and background is a full Compromise condition in exposure determination. It is necessary to consider the circumstances of photo subjects and backgrounds with variations of brightness, darkness, texture of surface and color for good result. This study was planned to analyze the results of pictures by a series of work, exposuring, developing and printing. A doll as a photo subject and four different backgrounds were set under the same lighting of a flood -lamp at a fixed distance for following comparison of influenced subject by contrast backgrounds. White Kent paper was the most bright background and black textile was extreme dark background. The medium grey textile and reflecting surface aluminium foil were used too. By measuring with light meter, the contrasts of brightness and darkness were checked up on the backgrounds. The white background was 16 times as bright a s black one, 4 times as bright as grey one and twice as bright as aluminium foil. The brightness of grey back was intermediate between whtie one and black one the former the most bright one and the latter the most dark one. The subject in white background was twice as bright as that in the other backgrounds because brightness of subject was influenced by brilliant background. In reflecting background, diffused reflection influenced upon subject partially. Under-exposed situation could result from extreme bright background or subject when they are exposed by average light metering without modification. On the other hand, over -exposed circumstances could come out of extreme dark background or subject by direct applications of measured light value without modification. It was difficult to measure the light under the condition of reflecting background, because diffused reflection interferes with light metering. We could check up the circumstances of over--exposure and under-exposure partially on the photographed subject caused by diffused reflection. The grey-tone background offers proper condition for subject on the whole. Following conaiderations are available for good result of photos A, Exposure of extreme bright or white subject and background has to be increased to prevent under-exposure. B, Exposure of extreme dark or black subject and background has to be reduced to prevent over -exposure. C, Partial light metering is superior to average light metering for correct exposure on the average. D, Background ought to be regarded as a photo subject to determine correct exposure.