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RISS 인기검색어
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- 저자 : 최병태(Byung Tae Choi) (검색결과 134 건)
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Regulation of Cyclin D3 by Calpain Protease in Human Breast Carcinoma MDA-MB-231 Cells
최병태,김군도,최영현,Choi, Byung-Tae,Kim, Gun-Do,Choi, Yung-Hyun Korean Society of Life Science 2006 생명과학회지 Vol.16 No.4
The $Ca^{2+}-activated$ neutral protease calpain induced proteolysis has been suggested to play a role in certain cell growth regulatory proteins. Cyclin proteolysis is essential for cell cycle progression. D-type cyclins, which form an assembly with cyclin-dependent kinases (cdk4 and cdk6), are synthesized earlier in G1 of the cell cycle and seem to be induced in response to external signals that promote entry into the cell cycle. Here we show that cyclin D3 protein levels are regulated at the posttranscriptional level by calpain protease. Treatment of human breast carcinoma MDA-MB-231 cells with lovastatin and actinomycin D resulted in a loss of cyclin D3 protein that was completely reversible by the peptide aldehyde calpain inhibitor, LLnL. The specific inhibitor of the 26S proteasome, lactacystin, the lysosome inhibitors, ammonium chloride and chloroquine, and the serine protease inhibitor, phenylmethylsulfonylfluoride (PMSF), did not block the degradation of cyclin D3 by lovastatin and actinomycin D. Results of in vitro degradation of cyclin D3 by purified calpain showed that cyclin D3 protein is degraded in a $Ca^{2+}-dependent$ manner, and the half-life of cyclin D3 protein was dramatically increased in LLnL treated cells. These data suggested that cyclin D3 protein is regulated by the $Ca^{2+}-activated$ protease calpain.
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Effects of Mycelial Extract of Phellinus linteus on Ethanol-Induced Liver Injury in Rats
최병태,최영현,길영기,Choi, Byung-Tae,Choi, Yung-Hyun,Gil, Young-Gi Korean Society of Life Science 2006 생명과학회지 Vol.16 No.6
We investigated the anti-inflammatory effects of mycelial culture extract from Phellinus linteus (MCPL) for suppression in the process of ethanol-induced inflammation in rat liver. Levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly increased in the serum of ethanol-treated rats compared to normal. However, the level of ALT was arrested markedly in ethanol-treated rats with MCPL compared to ethanol alone treated control ones. Severe histopathological changes of liver such as cloudy swelling, inflammatory cells infiltration, Kupffer cell reaction and focal necrosis were demonstrated in the rats challenged with ethanol compared with normal. Fewer scores of these changes were observed in MCPL-treated rat with recovered glycogen in centrolobular region of hepatic lobule. The Western analysis showed that the expression of inflammatory proteins such as cyclooxygenase (COX)-1, COX-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor $(TNF)-{\alpha}$ were increased in the ethanol-treated rat. But decline of COX-2 and iNOS expression were observed in MCPL-treated rat. Immunohistochemical analysis showed that the expression of COX-2 and $TNF-{\alpha}$ tended to increase in ethanol-treated rat, but decrease of these reactions were induced by MCPL treatment. These results suggest that MCPL may act as a protective agent for alcohol-induced liver injury through a regulating inflammation-related proteins.
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최병태(Byung Tae Choi),고우신(Woo Shin Ko),이용태(Yong Tae Lee),김경철(Gyeong Cheol Kim),이준혁(Jun Hyuk Lee),길영기(Young Gi Gil) 대한체질인류학회 1999 대한체질인류학회지 Vol.12 No.2
염증에서 iNOS 의 발현을 보기 위하여 흰쥐 정맥내 LPS 주사후 4시간,8 시간,12 시간에 뇌, 허파, 간, 심장을, 피부절제를 행한 후 5 일,10 일,15 일에 피부를 취하여 면역조직화학적으로 관찰하였다. LPS 주사군을 살펴보면 뇌에서 맥락엘기상피, 뇌실막세포에서 반응하며 일부 신경세포와 신경성유에서도 반응하는데 뇌들보와 시상하부에서 특히 높은 반응을 나타내었다. 허파에서는 허파파리큰포식세포, 허파꽈리세포, 민무늬근육세포 및 염증세포에서 반응이 나타났다. 간에서는 동굴모세혈관내 별큰포식세포와 더불어 염증세포에서 반응하며 일부 간세포와 쓸개관에서도 관찰되었다. 성장에서는 성장근육세포와 내피세포에서 관찰되었다. 이상의 반응은 LPS 투여후 8 시간에 높게 나타나며 뇌에서만 12 시 간에 다소 감소할뿐 다른 기관에서는 12 시간 까지 이러한 반응이 지속되었다. 피부에서는표피, 털뿌리의 상피뿌리집에서 반응을 나타내며 창상 치유 과정의 염증세포에서 강한 반응을 보이는데 특히 가피층아래 생괴사층에서 가장 강한 반응을 보였다. 반응정도는 창상치유 전과정에서 관찰되나 치유가 완료됨에 따라 줄어 들었다.
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