Paeonia anomala L.의 물 추출물이 3T3-L1 지방 전구세포의 분화 및지방 축적에 미치는 영향

최문열,김미형,김미려 대한본초학회 2024 大韓本草學會誌 Vol.39 No.2

Objective : Obesity is a disease caused by energy imbalance and increases the risk of complications such as cardiovascular disease and cancer. Additionally, a recent study reported that excessive production of ROS stimulates the expression of transcription factors related to adipogenesis, such as PPAR-γ, C/EBP-α and β, in 3T3-L1 cells. In this study, Paeonia anomala L. (PAL) was selected as a candidate for improving and preventing obesity from Mongolian medical literature, and its effect on fat metabolism was observed in vitro. Methods : The activity of PAL extract against lipid accumulation was measured in 3T3-L1 cells through Oil red O staining. Additionally, the expression of lipid metabolism-related proteins was measured by western blot. Results : Intracellular lipid accumulation was significantly lower in the treatment group than in the control group. In addition, pAMPK/AMPK protein expression in the treatment group tended to increase in a concentration-dependent manner, and PPAR-γ protein expression in the treatment group showed a significant decrease in a concentration- dependent manner compared to the control group. Conclusion : These results suggest that PAL extract may have an anti-obesity effect, and it is believed that it can be used to develop functional materials for the treatment of obesity.

액상 펄스 레이저 어블레이션에 의한 친수성 그라파이트 나노입자의 제조 및 센서 응용

최문열,김용태,Choi, Moonyoul,Kim, Yong-Tae 한국전기화학회 2012 한국전기화학회지 Vol.15 No.4

일반적으로 그라파이트는 높은 결정성으로 인해 나노입자상으로 제조하기 어려우며 특히 표면에 친수성을 부여하기가 쉽지 않은 재료로 알려져 왔다. 본 연구에서는 박막 증착에 널리 활용되어 오던 펄스 레이저 어블레이션 기법을 액상에 적용하여 친수성이 부여된 그라파이트 나노입자를 합성하였다. 타겟으로는 그라파이트 로드를 사용하였으며 레이저 출력을 조절하며 액상에서 어블레이션을 실시한 결과 매우 높은 분산 안정성을 갖는 친수성 그라파이트 나노입자를 합성할 수 있었다. FT-IR 분석결과 합성된 친수성 그라파이트 나노입자는 카르복시기 및 카르보닐기 등이 나노입자의 형성과 동시에 표면에 도입된 것이 밝혀졌으며 이는 제타 포텐셜로도 확인할 수 있었다. 최종적으로 Polyethyleneglycol(PEG)과 컴포지트하여 아세톤 센서에 적용한 결과 기존의 카본 블랙 대비 우수한 감도를 나타내었다. It is widely recognized that it is hard to prepare hydrophilic graphite nanoparticles because of their high crystallinity and inert characteristics. In this study, we successfully synthesized the hydrophilic graphite nanoparticles by using liquid phase pulsed laser ablation method which has been actively employed for the thin film deposition up to now. The obtained hydrophilic graphite showed an ultra-high dispersion stability in water, because the hydrophilic functional groups like carboxyl and carbonyl group was simultaneously introduced onto the graphite surface with the nanoparticle formation, as confirmed by FT-IR and zeta potential measurements. Finally, a markedly enhanced gas sensing ability for acetone was shown in comparison with the conventional carbon black for the carbon polymer composite sensor with polyethyleneglycol (PEG).

몽골산 약용식물 수종의 항비만 활성 탐색을 위한 기초 연구

최문열,김소영,김미려 대한본초학회 2023 大韓本草學會誌 Vol.38 No.3

Objective : Obesity in modern society has a significant impact on pathological, psychological and social problems. Therefore, many studies on obesity treatment using herbal extracts with fewer side effects have been conducted. This study was designed to investigate the effect of inhibiting fat accumulation in vitro in order to Mongolian medicinal plants find anti-obesity candidate among. Methods : We measured pancreatic lipase inhibitory activity in Mongolian medicinal plants extract. Cytotoxicity of these extracts was monitored in 3T3-L1 cells by MTT assay. In addition, the anti-obesity effects was confirmed at concentrations of 0.2, 0.1 and 0.05 ㎎/㎖ through Oil red O staining. Results : Among Mongolian medicinal plants, Rheum undulatum roots in September (RURS), Paeonia anomala L. (PAL), and Fragaria orientalis (FO) showed the highest pancreatic lipase inhibitory activity. As a result of the MTT assay, more than 80% was judged to be non-toxic, and the concentration was determined, and as a result of evaluating the lipid accumulation inhibitory effect, 6 types were selected as candidates. Conclusion : Based on these results, the top 7 species expected to be used as anti-obesity functional materials were selected. However, additional efficacy verification and mechanism of action need to be established in the future. So, it is expected that the medicinal plants verified through this will be used as functional materials for the prevention and treatment of obesity.

미숙감귤의 항산화 및 항노화 활성에 대한 평가

최문열 ( Moon-yeol Choi ),박해진 ( Hae-jin Park ),김재수 ( Jae Soo Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.1

Objective : This study was designed to investigate effect of immature fruits from Citrus unshiu Markovich to mature them on antioxidative and antiaging-related enzyme activities in vitro. Methods : Fruits from Open field-cultivated C. unshiu Markovich (ripe and unripe fruits of Citri) made a purchase in Jeju island. We measured total polyphenol and flavonoid contents in mature C. unshiu Markovich (MC) and immature C. unshiu Markovich (IMC) 70% ethanol extract. DPPH free radical, ABTS radical, and hydroxyl radical scavenging activities in both extracts were determined. Also, reducing power, SOD-like activities, elastase inhibition, and collagenase inhibition activities of these extract were assayed at 5, 1, 0.5, and 0.1 ㎎/㎖. Results : The total polyphenol contents in the ethanol extract of IMC were higher than those of MC (26.99 ± 0.89 ㎎/TAEg). And total flavonoid contents in them were very similar to both extract (MC; 7.25 ± 0.4 ㎎/RUEg and IMC; 7.44 ± 0.18 ㎎/RUEg). DPPH free radical scavenging activities, ABTS radical scavenging capacity, reducing power, and SOD-like activities of IMC-treated group showed significant increase compared to those of MC group in all treated concentrations. Particularly, DPPH free radical scavenging and elastase inhibition activities in IMC-treated group at 5 ㎎/㎖ showed similar with positive control group. Besides, IMC extract at 5 ㎎/㎖ concentration surpassed positive control (BHA or Vit. C) in SOD-like activities and ABTS radical scavenging capacity. Conclusion : These results suggest that IMC ethanol extract may be used as a natural antioxidant and a antiaging material for development of functional foods and cosmeceuticals.

C2C12 myotube의 산화적 손상에 대한 혼합 한약재 추출물(HME)의 Akt/FoxO3 신호 조절을 통한 보호 효과

김소영,최문열,이은탁,추성태,김미려 대한본초학회 2022 大韓本草學會誌 Vol.37 No.4

Objectives : In this study, we investigated the synergistic protective effects of medicinal herbal mixture (HME) including Mori Ramulus (MR), Acanthopanacis Cortex (AC), Eucommiae Cortex (EC), and Black soybean (BS) in C2C12 cells, mouse myoblasts. Methods : Effects of HME on cell viability of C2C12 myoblasts were monitored by MTT assay. Anti-atrophic activity of HME was determined in myoblasts and myotubes under oxidative stress by H2O2. C2C12 myoblasts were differentiated into myotubes in a medium containing 2% horse serum for 6 days. After that, we measured that expression of MyoD and myogenine, the myogenic regulatory factors, to identify the mechanism of inhibiting muscle atophy after HME treatment. In addition, suppression of phosphorylation of Akt, FoxO3a and MARF-1, transcription factors of degradation proteins were analyzed via western blotting. Results : As a result of MTT, HME there was no show cytotoxicity up to a concentration of 1 mg/ml. The cytoprotective effects on oxidative stressed myoblast and myotube was better in HME extract than those of MR, AC, EU, and BS, respectively. HME treatment in Myotube induced by oxidative stress after H2O2 treatment increased Myo D, Myogenine activation, and Akt, FoxO3a phosphorylation and decreased expression of MuRF-1. As the results, HME has synergistic effects on protection against proteolysis of C2C12 myotubes through activation of the Akt signaling pathway under oxidative stress. Conclusions : These results suggest that HME may also be useful as a preventing and treating material for skeletal muscle atrophy caused by age-related diseases.

Streptozotocin으로 유발된 흰쥐의 당뇨 증상 개선에 미치는 한약재 첨가 청국장 추출물의 영향

조창숙,김소영,최문열,김미형,고경아,김미려,서부일 대한본초학회 2022 大韓本草學會誌 Vol.37 No.4

Objective : This study was conducted to investigate antidiabetic effects of chunggugjang with medicinal herbal complex (CJ) in streptozotocin (STZ)-induced animal models. Method : STZ (65 mg/kg) was injected intraperitoneally to induce diabetes. Then rats were divided into 5 groups ; NG (normal diet + 0.9% saline), COS (STZ +saline 5 mL/kg), COB (STZ + fermented soybean(100 mg/kg), CJ 100/200 (STZ+CJ(100 and 200 mg/kg), CJ 300/600 (STZ+CJ(300 and 600 mg/kg). 4 weeks later, oral glucose tolerance test (OGTT) was performed. After sacrificing rats, serum levels of blood urea nitrogen (BUN), creatinine, aspartate amino transferase (AST), alanine amino transferase (ALT), total cholesterol (TC), and triglyceride (TG) were measured and histological changes were observed. Result : Body weight change and food efficiency ratio (FER) were decreased in the CJ 300/600 group than in the COS group. But, there was no change in water intake. Serum levels of glucose, AST, ALT and BUN were lower in the CJ 300/600 group than in the COS group. Also, TG, TC, and creatinine were decreased in the CJ 300/600 group than in the COS group. According to OGTT, 120 minutes postprandial glucose levels were lower in the CJ 300/600 group than in the COS group. In addition, administration of CJ extracts restored histopathological damage. Conclusion : The results suggest that CJ can be used as a functional material for diabetes treatment as it has the effect to improve pathological symptoms in STZ-induced diabetic rats. 1

우슬의 에탄올 추출물이 모발의 흑화에 미치는 영향

이미자 ( Mi-ja Lee ),최문열 ( Moon-yeol Choi ),김유진 ( Yoo Jin Kim ),김미려 ( Mi Ryeo Kim ),유왕근 ( Wang Keun Yoo ) 대구한의대학교 제한동의학술원 2021 東西醫學 Vol.46 No.1

Objective : a-MSH (alpha-melanocyte-stimulating hormone) and IBMX (3-lsobutyl-1-methylxanthine) were known as regulating melanogenesis and differentiation, but they were restricted usage because of stability. So, natural products have developed about regulating melanogenesis and differentiation. Therefore, we investigated about effect of Achyramthis Radix (AR) ethanol extract on gray hair. Methods : a-MSH was used as a positive control. We set up the safe concentration range by MTT assay and conducted melanin contents and tyrosinase activity in mouse melanoma (B16F10) cells. Results : As results, melanin contents were increased in Achyramthis Radix ethanol extract (AR) group contrast to control group. Also, AR group is higher than control group on tyrosinase activity. Conclusion : Our results suggest that AR could be used as a potential material for the treatment of gray hair by increasing the melanogenesis of B16F10 cells.

우슬의 에탄올 추출물이 모발 성장에 미치는 영향

이미자 ( Mi-ja Lee ),최문열 ( Moon-yeol Choi ),김유진 ( Yoo Jin Kim ),김미려 ( Mi Ryeo Kim ),유왕근 ( Wang Keun Yoo ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.4

Objective : As more and more people are interested in appearance in modern society, the increasing number of hair loss population can have an important impact on psychological and social problems such as depression and inappropriate interpersonal symptoms. Therefore, much research is being done on treatments for alopecia using herbal extracts with relatively few side effects. This study was investigated about the effect of Achyranthis Radix (AR) extract with ethanol solvent on hair growth. Methods : We determined the promoting efficacy of AR-ethanol extract compared with minoxidil (MNXD) on the growth of human hair dermal papilla cells (HDPCs). Cell viability was measured by MTT assay and cell proliferation was confirmed by cell cycle analysis from flow cytometry in HDPCs. Also, we monitored the safe concentration range through MTT assay. And protein expression of hair growth-related genes (insulin-like growth factor 1 (IGF-1), Wnt3a, Protein kinase B (Akt), Extracellular signal-regulated kinase (Erk)) was monitored by western blot. Results : On cell cycle analysis, the G2/M phase was higher than that of the DW group in AR ethanol extract group at 0.05 and 0.1 ㎎/㎖. All protein expression levels of HDPCs were increased in AR ethanol extract groups and the MNXD group, compared to the DW group, respectively. Conclusion : As mentioned above, AR extract increased cell proliferation and the protein expression of IGF-1, Wnt3a, Akt, Erk in HDPCs. These results suggest that AR ethanol extract has promoted hair growth and it might be potential hair growth supplement.

추출 용매를 달리한 생강 추출물에 대한 생리활성의 비교 평가 연구

김소영 ( So Young Kim ),최문열 ( Moon-yeol Choi ),도은주 ( Eun Ju Do ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.2

Objective : This study was designed to investigate anti-oxidative, anti-aging and anti-inflammatory activities of Zingiber officinale Roscoe water (ZD) and 50% ethanol extracts (ZE). Method : The antioxidant capacity of ZD or ZE was analyzed by DPPH, ABTS scavenging activities and reducing power, respectively. The anti-aging efficacy was performed with tyrosinase and collagenase inhibition activities. The anti-inflammatory activities were confirmed through inhibition of nitric oxide production and TNF-α expression in LPStreated Raw 264.7 cells. Result : As a result of measuring DPPH and ABTS radical scavenging activities, both ZD and ZE extracts increased in concentration-dependent manners. The ABTS radical scavenging activities of water and 50% ethanol extract were similar to that of positive control at high concentration. On reducing power, both ZD and ZE showed higher activities than the positive control at high concentration. On inhibition activities of tyrosinase and collagenase both ZD and ZE showed the large inhibition rates at low concentration. Also, the inhibitory efficacy of ZD on NO and TNF-α production was better than ZE in RAW 264.7 cells. Conclusion : These results suggest that ZD and ZE have anti-oxidative, anti-aging and anti-inflammatory effects in vitro. Especially, ZD was more effective than ZE. Thus, Ginger extract is considered to be highly useful as a cosmetic or food material with anti-oxidative, anti-aging (prevention of whitening and wrinkle) and anti-inflammatory effects. Also these effects may vary depending on the extraction solvent.

기업설문 조사를 통한 약선 HMR의 필요성과 수요자 맞춤형 제품개발 방향설정 연구

박해진,이정훈,장재기,유왕근,한삼성,최문열,노성수,박순애,김미림 한국식생활문화학회 2019 한국식생활문화학회학술대회논문집 Vol.2019 No.11