經濟開發計劃과 企劃擔當者 能力分析

최두식 서울大學校 行政大學院 1970 국내석사

HL-60 세포에서 H2B histone 유전자 발현에 대한 Octamer binding transcription factor의 역할

최두식 충남대학교 1995 국내석사

To gain insight on the role of octamer binding transcription factor (OTF) in the transcriptional regulation of the H2B histone gene expression in HL-60 cells, the binding pattern of OTF to octamer motif in the human H2B histone gene upstream region has been investigated with DNase I footprinting and DNA mobility shift assay. One nuclear factor bound at -42bp (octamer motif), Two DNA-protein complexes (OTF-1A 8z 1B) were formed by DNA mobility shift assay when octamer element was incubated with nuclear extract of HL-60 cells. Although the DNase I protection pattern by nuclear extract of the cycloheximide (CHX)-treated HL-60 cells showed the same result as CHX-treated HL-60 cells, the decrease of OTF level was detected by DNA mobility shift assay. The levels of OTF are unchanged during cell cycle and OTF does not require synthesis of new proteins during the S phase. These results suggest that cell cycle-specific posttranslational modification of OTF may be responsible for the S phase-dependent transcription of H2B histone gene.

회분식 장치에서 미생물을 이용한 고농도 액상산업폐기물의 탈질 특성

최두식 한국교통대학교 일반대학원 2019 국내박사

본 연구는 500mℓ의 Bioreactor에 합성 폐액 360mℓ와 생물종자 40mℓ를 혼합하여 400mℓ의 실험 용액을 만들어 일정시간 반응시켜 시료를 채취하여 pH 변화, 온도의 영향, 미생물의 성장, 질소의 제거 등을 분석하였다. 실험 조건은 질산성 질소의 농도는 10,000 ∼ 25,000 mg/ℓ, pH의 범위는 6.0 ∼ 9.0, 사용된 용액은 Ammonium nitrate(NH4NO3), Sodium nitrate(NaNO3), A/N(Acetic acid/Nitrate)비는 0.8, 1.0, 1.2, 미생물의 농도는(Biomass concentration) 10 ∼ 50 %, 전자공여체(Electron donor)는 CH3COOH, 온도는 25 ∼ 35 ℃의 변화를 주었으며, 미생물은 Pseudomonas halodennitrificans를 이용하였다. 실험 방법은 Batch reactor에서 반응을 시켜 일정 시간이 경과 후에 주사기를 사용하여 시료를 채취하고 pH를 측정한 후, 광학밀도를 측정하였다. 또한, Microfilter와 원심분리기를 이용하여 시료를 고·액 분리하였고 분리된 액체에서 질소의 농도를 분석하고 분리된 고체는 하루동안 60 ℃ 건조기에서 건조한 후 MLSS(Mixed liquor suspended solid)를 분석하고, 600 ℃에서 30분간 연소시킨 후 MLVSS(Mixed liquor volatile suspended solid)를 분석하였다. 질산성 질소를 함유한 고농도 액상산업폐기물의 발생억제와 효율적인 처리를 위한 생물학적 탈질을 연구한 결과는 다음과 같다. - 운전 가능 pH 범위는 6.5 – 8.5, 최적 운전 온도는 30℃, A/N 비는 1.0이 최적 조건이며, 미생물의 량을 최대로 유지하는 것이 바람직하고 Nitrate 양에 관계없이 생물학적 처리가 가능하다. - Ammonium nitrate(NH4NO3) 용액의 탈질 속도는 3.55g/l.day, MLSS는 2.75g/l, MLVSS/MLSS는 83.9%, 질소제거 효율은 96.7 % 이다. - Sodium nitrate(NaNO3) 용액의 탈질 속도는 3.24g/l.day, MLSS는 2.83g/l, MLVSS/MLSS는 78.1, 질소제거 효율은 95.9 % 이다. - Ammonium nitrate(NH4NO3)와 Sodium nitrate(NaNO3) 용액의 kinetic parameter는 모두 문헌치 범위인 것으로 나타났다. - 탈질용액의 생물학적 처리를 고려하면 recycle이 없을 때는 Ammonium nitrate(NH4NO3) 용액이 효율적이고, recycle이 있는 경우 Sodium nitrate(NaNO3) 용액이 효율적이다. This study carried out the denitrification of liquid industrial wastes containing high concentration nitrate nitrogen by using microorganisms in a batch reactor. A simulated solution was used as the liquid industrial waste. The experiment was carried out by mixing 360 ㎖ of the simulated solution with 40 ㎖ of the microbial seed solution. Experiments were carried out in a batch reactor for a certain period of time, and samples were taken for pH change, effect of temperature, growth of microorganisms, and concentration of nitrate nitrogen. The experimental conditions were as follows: the concentration of nitrate nitrogen was 10,000 ~ 25,000 mg / L, the pH range was 6.0 ~ 9.0, the solutions used were AN (NH4NO3), SN (NaNO3), A / N ratio was 0.8, the concentration of Pseudomonas halodennitrificans was 10 ~ 50 %, the electron donor was CH3COOH, the temperature was 25 ~ 35 ℃, and the microorganism was Pseudomonas halodenitrificans. In the experimental method, samples were sampled using a syringe after a certain reaction time, pH was measured, and optical density was measured. The concentration of nitrogen in the separated liquid was analyzed by using a microfilter and a centrifugal separator. The separated solid was dried in a dryer at 60 ℃ for one day, analyzed for MLSS, and burned at 600 ℃ for 30 minutes and MLVSS were analyzed. The following conclusions were obtained from this study. - The operating pH range is 6.5 - 8.5, optimum operating temperature is 30 ℃, and A / N ratio is 1.0. It is desirable to maintain the maximum amount of microorganisms and biologically treatable regardless of nitrate concentration. - The denitrification rate of ammonia nitrate (NH4NO3) solution is 3.55, MLSS is 2.75g/l, MLVSS / MLSS is 83.9 %, and nitrogen removal efficiency is 96.7%. - The kinetic parameters of ammonia nitrate (NH4NO3) and sodium nitrate (NaNO3) solutions were all in the literature range. - Considering the biological treatment of the denitrification solution, the ammonium nitrate (NH4NO3) solution is efficient when there is without recycle, and the sodium nitrate (NaNO3) solution is more effective with recycle.

春香傳 構造 一考察

최두식 建國大學校 大學院 1979 국내석사

In this essay, I attempted to analyze the structure of Chunhyangjon, the famous Korean classic in Yi dynasty, according to the theories of J.B. Fages and Levi Straus. But this research is merely a part of the structual analyses. The outline of the research is as following: 1. The hero Lee Mong Yong and the heroine Chun Hyang had heterogeneous factors. For the hero's part, there can be found obstructing factors in their combination. 2. The date of their combination was Danohjeol(端午節), a festival day of May by the lunar calendar, when all creations are most animated every year. Lee Mong Yong did not know the true meaning of the festival. 3. The place of their combination wao Kwanghallu(廣寒樓) and Ohjakkyo(烏鵲橋). The name, Kwanghallu, which is derive from Taoism, has vertical meaning. It means connecting the heaven and the earth. Lee Mong Yong did not recognize the vertical meaning in this respect. He lost the Yangban's(兩班) prestige and leadership. The name of Ohjakkyo is derived from folktale. The folktale says; Gyon Woo(牽牛) a man driving the ox, and Jiknyo(織女), a woman weaver, meet only once a year at Ohjakkyo bridge on July 7 by lunar calendar. This bridge has the horizontal meaning, which Chung Hyang perceived in its full sense. She had well-rounded character, tolerance, and self-purification, as if the water flowing under the bridge had. 4. The vehicle of their combination is Wolmae's(月梅) dream. But Lee was a man of no dreams. 5. Chun Hyang's social position is promoted by her will heading for virtue. And her promotion of social position means the rising from the position of humbles to that of commons 6. Chun Hyang's suffering from whip execution and imprisonment have much to do with the elements of Dangunshinwha(Korean national foundation myth); whip execution is associated with garlic and wormwood, and imprisonment, with cave. 7. In the strict sense, Lee Mong Yong has neither conclusive virtue, nor has Byunsatto (卞使道) conclusive vice.

韓國詠史文學硏究

최두식 건국대학교 대학원 1987 국내박사

History has been a matter of great interest to human beings. It is inevitable that any individual has his own personal history, and. that any society has its own history, and that any country has its, own vicissitude in history. In this respect men recognize and create their own history with much concern for it, and so they are generally considered as a historical animal. There is no denying the fact that only Korea and China have taken too much interest in their own histories in particular compared with any other countries in the Oriental civilization, even though the interests in history are common to both the East and the West Judging from this, we may remember that the writings of biographical style in various individual collections and the genealogical writings were rampant in the past, and that would prove vividly the concern for the individual records and the historical records of family at that time. Our Korean ancestors have recorded and developed their biographical writings to say nothing of their genealogical ones, but in addition to this, they have been greatly interested in the rise and the fall of a certain dynasty, and as a result of this, the various historical books of many historians have been gushed out. In this respect it may be that history should be given much more weight in academic field, because it may be no exaggeration to say that the knowledges of the past came from only these historical writings. Therefore it might be recognized that the test of intellectual character in man was his own historical knowledge. By the way, most of historical writings were written in the Chinese characters, and they were as good as the monopolistic possessions of the Confucian scholars. Because all the common people couldn't write and read those works without the knowledge of the Chinese characters when the Hankul or the Korean alphabet has not yet been invented. The work which these defects of historical writings' is Youngsa(詠史). Moreover a prodigious amount of historical writings recorded in the Chinese characters were preserved in the book storehouse and could be of little practical use. These historical writings left unused were mostly transformed into the poetic works, oun-shi(五言詩: classical Chinese verse which has five letters to one line) or chirun-shi(七言詩: classical Chinese verse which has seven letters to one line), and sung by the educated Confucian scholars or intellectuals, and these records were gradually of practical use. These efforts could be found in Chonjayokdaeka(千字歷代歌) or any other writings, which were given their stress on study of characters and on cultivation of historical consciousness. Youngsa can be divided into two types: the short form and the long form. In general the former is lyrical dealing with a single person or even, twhile the latter is epic dealing with numerous persons or events. And so it may be considered that the short form could' be changed into the long form. Youngsa in long form was written in classical poetic style, oun-shi, ckirun-shi, and or so, and became the basis of Kasa(歌辭: a kind of long formed work, so called jangka); and among youngsas Yokdaejunli-ka(歷代轉理歌) in the Chinese characters and idu(吏讀) inscription could be considered as the similar to kasa. There are some in long formed youngsas; A Reminiscent Poem of Twenty-One Capitals(二十一都□古詩) which is divided partly by capital, and Yongbiochun-ka(龍飛御天歌) which is divided chapterly by each deed, and A Historical Tale of Yi Dynasty for Five Hundred Years(李朝五百年史話) divided by each king per chapter. And the scanning way of these poetic works is not against that of the long formed youngsa. As Wookuk kyongshi-ka(憂國警詩歌) and its similar modes are divided partly on the basis of the singing way of changka (唱歌), its singing way is the different from those of the above mentioned writings Such as this, the poetic work which was divided partly in accordance to the singing way of changko, appeared, but the long formed youngsa went its own way, and its famous example is Historical Songs' about Five Thousand Years. In' general youngsa has an episodic plot(□殷性的 結構方式) Yokdae-ka, Hanyang-ka(漢陽歌), and Historical Songs about Five Thousand Years(半萬年史歌) show the rise and the fall of dynasty successively in chronological order. Its way of writing is similar to a chronicle style. But in Daemyongboksu-ka (大明復□歌) the undesirable history in the past is rewritten into the good and justifiable one through the form of youngsa, and so it has a charm in its plot, but it is every bit a fiction. As the authors of youngsa were mostly the Confucian scholars, they took the serious view of, the historical truth. So they didn't fabricate its truth at all, and they sang only the truth. Its good examples are Yokdae-ka and so on. This work has some problems in the literary aspect that its plot is flat not dramatic. Aristotle talks about non-plot in his Poetics that the episodic plot is the worst of all. The complete and familiar plot, however, makes the readers incapable, and so it is very fortunate for the readers that youngsa have some non-plot aspects. There fore I may, be able. to find their dramatic plots not in the works themselves but in the heart of the readers when they read them. The authors of youngsa were the scholars and intellectuals in the educated class, who could be accessible to the historical records. Most of those who wrote youngsa in the Chinese poetic form are identified while the authors who wrote them in Korean are anonymous. Especially as Hanyang-ka and other works under its category have many different versions, it is not so easy to identify their authors. Taking the fact into account that many transcribers or collectors have continued to revise, correct, and erase the considerable parts of works, the living different versions are said to belong to many readers who have taken part in, their writing. Because most of, transcribers or collectors were readers themselves. The original author, however, was sure to be one writer. The views of the writers in youngsa are divided into two attitudes the attitude to affirm the standpoint of the mystery in the ancient history and attitude to deny it. As the ancient society, so called the sacred community was in the period when the religious orders or the mysterical orders dominated, much more weight on the deeds in the ancient community could be found in the ancient history. In King Dongmyong(東明王篇) and Jewang-woonki(帝王□紀) their authors praised the mysteriousness of the founding of the ancient state affirmatively through the poetic lines of strong mythological tints. The affirmative attitude on this mysteriousness developed into the views on the Heaven worship, and many events on the Creation of Heaven and Earth were treated very importantly in youngsas of the Chinese ancient history. By the way on the historical viewpoint of social evolution considering how the sacred community had developed into the secular society, the people in those days denied the mysterious community and affirmed the substantial society, and moreover they pursuited the scientific reasonableness rather than the religious orders, and did the reasonable intellect rather than the unreasonable mysteriousness. That is to say, the writers of youngsas from the class of the scholars assumed the negative attitude of the strange mythical happenings with the thought of Confucian rationalism. In the case of A Reminiscent Poem of Twenty-One Capitals we can find the affirmative views on the holiness of Dankun-chosun(檀君朝鮮) and King Dongmyong not the mythical aspects. The thought of the Heaven worship produced the manners to take a serious view of Providence under the monarchism, and became one of the most important consciousness of orders presented in Yokdae-ka and other works under its category. Then this Heaven worship attitude leaned toward Docham-Poongsu-sol(圖□風水說, a kind of Oriental geomantic theory) and divination. We can see this aspect in the part of Yongbiochun-ka which the sign of the founding of Chosun was sung, and in the part of Hanyang-ka which the deeds about the transfer of the capital to Hanyang were sung. This thought changed into humanism by the action of the view of life and universe based on the life force, and this exemplifies that the best spirits of mountains and rivers are supposed to bring forth the able men with their own efforts in Yokdae-ka(with special reference to cultural history) of Akbu-kajip(樂府歌集). And the main idea in youngsa is Buddhist thought with special respect to the guiding principle of Confucius. In consequence of this, the spirit of resistance to the savage foreigners and the anti-Japanese sentiment came to the fore with the tendency to guard against the heretical views to Confucianism and the Buddhism. It may be suggested that it is the expression of the anti-savage foreigners that youngsa deals with the deeds from the ancient age to Ming period except those in Ch'ing period. Hwadong-yokdae-ka(華東歷代歌), Yokdae-chonja(歷代千字), Yokdae-yichonia(歷代二千字), and Daemyongboksu-ka deal with the events and the deeds up to the end of Ming period. It is the distinctive feature of youngsa that though it deals with descriptive aspect, it doesn't include the heroic action of historical character. The characters in this kind of work are the passive persons who act in accordance to Providence not human nature. The warlike character with heroism are not allowed by the reason of being against the guiding principle of Confucius. Therefore we can't find out the scenes of war or battlefield in those works, and this rests on the basis of aversion to militarism. The cruelty of revenge in Daehanboksu-ka(大韓復□歌) is supposed to be not realistic but fictious, and it is only author's subconscious product, not bellicosity.

사회적 지원에 대한 인식이 직무만족과 성과에 미치는 영향 : 리더십 유형을 중심으로

최두식 경희대학교 대학원 2019 국내박사

In order to survive in a competitive, rapidly changing political, social, and economic environment, the organization is seeking various measures such as improving technology and marketing. In human services, attention is also increasingly focused on the measures of securing and retaining, and of preventing drains of talented people who have acquired professional knowledge and skills and have a sense of professionalism. In particular, employees who provide services at customer contacts must interact with customers at all times, and should face customers in a courteous manner in the process of delivering services. However, there is a case they do not take proper attitudes and this situation causes imbalance between the employee and the customer, and are exposed to the stress. Employees who provide services at customer contact experience intense job stress through this process, and ultimately affect organizational effectiveness such as job performance and turnover intention. Organizations are managed through internal factors such as personality type and self-efficacy, external factors such as social support and control, to reduce job stress. In particular, the social support - an external factor - may be referred to a factor that draws the interests of researchers in recent years as a factor affecting job satisfaction as well as relieving job stress. In this study, we examined the effects of recognition of social support on job stress, job satisfaction and job performance, and investigated whether leadership style plays a role as a control variable, with business line and size controlled, in order to demonstrate empirically how the social support impacts on job performance by weakening the negative impact on job stress and increasing job satisfaction. The results are as follow: First, social support had a negative (-) effect on job stress, so job stress decreased as social support increased. As a result of examining the moderating effects of leadership style on the influence of social support on job stress, it was found that transactional leadership and servant leadership, among leadership styles, play roles as control variables. Second, social support had a positive effect on job satisfaction, so it was found that job satisfaction increased with the increase of social support. As a result of examining the moderating effects of leadership style on the influence of social support on job stress, it was found that servant leadership, among leadership styles, plays a role as a control variable. Third, social support had a positive effect on job performance, so it was found that job performance increased with the increase of social support. As a result of examining the moderating effect of leadership style on the effect of social support on job performance, transactional leadership, transformational leadership and servant leadership, among leadership styles, played roles as control variables. Fourth, job stress had a negative (-) effect on job performance, so job performance decreased as job stress increased. As a result of examining the moderating effect of the leadership styles on the relationship between job stress and job performance, only servant leadership, among the leadership styles, played a role as a control variable. Fifth, job satisfaction had a positive effect on job performance, so job performance increased as job satisfaction increased. As a result of examining the moderating effects of the leadership styles on the relationship between job satisfaction and job performance, it was found that the transformational leadership and servant leadership, among the leadership styles, played roles as the controlling variables. 조직에서는 경쟁이 치열하고 급변하고 있는 정치ㆍ사회ㆍ경제ㆍ환경 등에서 생존하기 위해 기술력 향상이나 마케팅 활용 등 여러 가지 방안을 모색하고 있으며, 인적 서비스도 전문적 지식과 기술을 습득하고 직업의식이 투철한 인재의 확보 및 유지방안과 유출 방지방안에 대한 관심도가 높아져 가고 있다. 특히, 고객접점에서 서비스를 제공하고 있는 종사원의 경우 항상 고객과 상호작용을 해야 하며, 서비스를 전달하는 과정에서 예의바른 태도로 고객을 대해야 한다. 하지만 예의바른 태도를 취하지 않는 경우가 발생하게 되고 이러한 상황으로 인하여 종사원과 고객 사이의 불균형을 초래하게 되어 스트레스에 노출되게 된다. 고객접점에서 서비스를 제공하는 종사원들은 이러한 과정을 통해 강도 높은 직무스트레스를 경험하게 되며, 궁극적으로 직무성과 및 이직의도와 같은 조직유효성에 영향을 미치게 된다. 조직에서는 이와 같은 직무스트레스를 감소시키기 위해 성격유형 및 자기효능감과 같은 내적요인과 사회적 지원 및 통제소재 등과 같은 외적요인을 통해 관리하고 있다. 특히 외적 요인인 사회적 지원은 직무스트레스를 해소시켜줄 뿐만 아니라 직무만족에도 영향을 미치는 요인으로 최근에 연구자들에 의해 관심이 높아져 가고 있는 요인이라 할 수 있다. 본 연구에서는 사회적 지원이 직무스트레스에 미치는 부정적인 영향을 약화시키고, 직무만족을 높여 직무성과에 어떠한 영향을 미치는지를 실증적으로 검증하고자 기업업종, 기업규모를 통제한 상태에서 사회적 지원에 대한 인식이 직무스트레스, 직무만족과 직무성과에 미치는 영향관계를 살펴보면서 리더십 유형이 조절변수로서의 역할을 하는지 살펴본 결과, 첫째, 사회적 지원이 직무 스트레스에 부(-)의 영향을 미치는 것으로 나타났기 때문에 사회적 지원이 커질수록 직무 스트레스가 감소(-)함을 알 수 있었다. 사회적 지원이 직무 스트레스에 미치는 영향관계에서 리더십 유형의 조절효과를 살펴본 결과 리더십 유형 중에서 거래적 리더십과 서번트 리더십이 조절변수로서의 역할을 하는 것으로 나타났다. 둘째, 사회적 지원이 직무만족에 긍정적(+)인 영향을 미치는 것으로 나타났기 때문에 사회적 지원이 커질수록 직무만족이 증가(+)함을 알 수 있었다. 사회적 지원이 직무만족에 미치는 영향관계에서 리더십 유형의 조절효과를 살펴본 결과 리더십 유형 중에서 서번트 리더십이 조절변수로서의 역할을 하는 것으로 나타났다. 셋째, 사회적 지원이 직무성과에 긍정적(+)인 영향을 미치는 것으로 나타났기 때문에 사회적 지원이 커질수록 직무성과가 증가(+)함을 알 수 있었다. 사회적 지원이 직무성과에 미치는 영향관계에서 리더십 유형의 조절효과를 살펴본 결과 리더십 유형 중에서 거래적 리더십, 변혁적 리더십과 서번트 리더십이 조절변수로서의 역할을 하는 것으로 나타났다. 넷째, 직무 스트레스가 직무성과에 부(-)의 영향을 미치는 것으로 나타났기 때문에 직무 스트레스가 커질수록 직무성과가 감소(-)함을 알 수 있었다. 직무 스트레스가 직무성과에 미치는 영향관계에서 리더십 유형의 조절효과를 살펴본 결과 리더십 유형 중에서 서번트 리더십만이 조절변수로서의 역할을 하는 것으로 나타났다. 다섯째, 직무만족이 직무성과에 긍정적(+)인 영향을 미치는 것으로 나타났기 때문에 직무만족이 커질수록 직무성과가 증가(+)함을 알 수 있었다. 직무만족이 직무성과에 미치는 영향관계에서 리더십 유형의 조절효과를 살펴본 결과 리더십 유형 중에서 변혁적 리더십과 서번트 리더십이 조절변수로서의 역할을 하는 것으로 나타났다.

블록 암호 알고리즘에 대한 라운드 축소 오류 주입 공격

최두식 호서대학교 2012 국내석사

블록 암호 알고리즘(AES, Triple DES)을 사용하고 있는 암호 디바이스에 오류를 주입하여 정상적인 블록 암호의 라운드가 실행되지 못하도록 하고, 이를 통해 얻은 오류 암호문과 정상 암호문을 이용하여 블록 암호 알고리즘에서 사용하는 비밀키 추출한다.

하수처리 구역 내 공공하수도 원인자부담금의 합리적 방안연구

최두식 忠州大學敎 産業大學院 2005 국내석사

우리나라 貿易의 부가가치구조

최두식 동국대학교 대학원 1998 국내석사

HL-60 세포에서 12-O-tetradecanoyl-phorbol-13-acetate에 의한 DNA topoisomerse Ⅱα 유전자 전사조절에 관련된 trans-acting factors의 특성에 관한 연구

최두식 충남대학교 대학원 1998 국내박사

DNA topoisomerase IIa plays an important role in events leading to mitosis and Topo IIa gene is regulated at cell-cycle dependent manner. To gain insight on the mechanism of transcriptional repression of Topo IIa gene, the binding pattern of nuclear proteins to various cis-elements in the Topo IIa gene upstream region has been investigated with DNase I footprinting and DNA mobility shift assay. HL-60 cells were arrested at G_/G_ phase after TPA treatment in FACS analysis. The level of Topo IIa mRNA and unknotting activity were reduced at 24 hours after exposure with TPA. The unclear factors, were which newly induced during reduction of Topo IIa mRNA after TPA treatment, bound with at least four sites : I-CCAAT-1 at -68 bp, I-CCAAT-2 at -108 bp, I-CCAAT-4 at -259 bp and novel cis-element at -196 (Topo IIa specific sequence, TSS : upper strand, 5-TAGTCCCGCCTCCCTAACCT-3). The DNase I protection pattern showed the same result with the control after addition of competitors including TSS and I-CCAAT. DNA mobility shift assays using oligonucleotides containing the I-CCAATs showed one specific DNA-protein complex and the competition studies indicated apparent binding of ICB-1~4 to the same protein while ICB-5 bound to a different protein. DNA mobility shift assays using oligonucleotide containing the TSS showed two specific DNA-protein complexes and the TSSBP bound to TSS-specific and novel trans-acting factor. TPA-dependet induction of TSS binding protein (TSSBP) reduced after pretreatment of staurosporin and completely vanished after pretreatment of cycloheximide. These results suggest that the transcriptional repression of Topo IIa gene during TPA-induced differentiation of HL-60 cells may be mediated by four transacting factors that are newly induced by TPA and TSSBP is a novel trans-acting factor.