International Solanaceae Genome Projects: The Korean Efforts on Pepper and Tomato Genomics

최도일 한국육종학회 2008 한국육종학회 학술발표회 발표요지 Vol.40 No.-

농산물 물류표준화 통한 국제경쟁력 제고

최도일 (사)한국포장협회 2004 包裝界 Vol.134 No.-

제4차 국제 가지과 유전체 워크숍을 마치고…

최도일 한국원예학회 2008 한국원예학회 학술발표요지 Vol.2008 No.5

A Method of High Frequency Virus-induced Gene Silencing in Chili Pepper (Capsicum annuum L. cv. Bukang)

최도일,정은숙,성은수,김영철,정은주,오상근,이상협,박정미,정영희 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.2

Using a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system, expression of phytogene desaturase (PDS) and ribulose-1,5-bisphosphate car-boxylase small-subuit (rbcS) genes was suppressed in Nicotiana benthamiana and pepper plants (Capsicum annuum L. cv. Bukang). The silenced phenotypes of pale yellow (rbcS), and photobleached leaves (PDS), were invariably obvious 2 weeks after inoculation with the TRV-based vector. In a parallel experiment, the same set of genes was silenced in N. benthamiana and yielded identical phenotypes to pepper 1 week after in-oculation. Northern blot analyses showed that the en-dogenous levels of CarbcS and CaPDS transcripts were dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. To our knowledge, this is the first high frequency VIGS method in pepper plants. It should provide a tool for large-scale gene silencing studies in pepper functional genomics.

초오가 포함된 단방약에 의한 Aconitine 중독

최도일,진영호,이재백 대한응급의학회 2002 대한응급의학회지 Vol.13 No.2

Purpose: Preparations of Aconitum roots in Chinese and Korean medicine are carefully prescribe for analgesic, antirheumatic and neurologic indications. However, in Korean folk medicine, a crude medical herb prepared from Aconitum is still used as form of a decoction or an unrefined tablet. Potential hazards of these medicinal herbs are poisoning and occasional fatalities due to low margins of safety. This study was conducted to document the adverse effects related to the use of these folk recipes that resulted in the emergency department(ED) admission. Methods: A retrospective survey was conducted of 30 patients presented to the ED of a tertiary University Hospital with suspected adverse effects from folk recipes containing Acontium over a three-year period from 1999 to 2001. The patients was analyzed according to sex, age, intention of use, residential area, vital signs on ED presentation, time to prsentation, time to discharge or ECG normalization, frequency of chief complaints, and ECG abnomalities. Results: The patients mean age was around 67 years old. The patients were predominantly female (22 patients) and from rural areas (21 patients). The drug effects of Aconitum were not appreciated by almost all the patients. Of the chief complaints, nausea or vomiting was most common (76.7%). Time to presentation in the ED varied (30∼930 minutes), which suggests that the occurrence of symptoms is directly related to the amount of aconitine in folk recipes containing Aconitum. Upon the arrival, most of the patients were hypotensive (MVP: 62 mmHg), but heart rates were normal or tachycardic. The ECG findings were variable, and, except for one case, normal sinus rhythm was restored within 24 hours without pharmacological intervention. Conclusion: This research demonstrates the need to emphasize public announcement to deter the injudicious use of folk recipes containing Aconitum species.

통증에 대한 단일차원 평가법과 구두 표현법을 이용한 통증경감의 임상적 의미

최도일,정태오,진영호,이재백 대한응급의학회 2003 대한응급의학회지 Vol.14 No.1

Purpose: The visual analogue scale (VAS) pain score is widely and frequently used to evaluate the severity of pain. However, statistically significant differences in the VAS scores may not always mean differences in pain severity. This study is to determine clinically meaningful reductions in pain severity as measured by the VAS and by a verbal categorical rating of pain. Methods: Three hundred adult patients presenting to the emergency department (ED) with acute pain resulting from trauma or non-traumatic diseases were enrolled in this prospective, descriptive study. A 100-mm non-hatched, horizontal visual analogue scale was used to measure pain severity. The VAS measurements were obtained two times 1 minute apart at admission, 30 minutes after admission, and 1 hour after treatment. At each VAS measurements, patients also gave verbal ratings of their pain as "more pain," "the same pain," or "less pain." Data from the groups reporting "the same pain" or "less pain" were compared with their preceding descriptions and yielded a VAS difference. The mean VAS change was calculated, from which a grand means and 95% confidence intervals (95% Cl) were determined. Results: At 30 minutes after admission and 1 hour after treatment, 256 and 31 patients, respectively described their pain as "the same pain," and 33 and 269 patients described it as "less pain." The mean reduction in VAS for the group reporting that pain was "the same pain" was 13 to 16 mm (95% Cl, 8 to 20 mm) instead of 'zero.' For the group reporting that pain was "less pain," the mean reductions in VAS score were 24 mm (95% Cl, 20 to 28 mm) at 30 minutes after admission and 44 mm (95% Cl, 42 to 46 mm) at 1 hour after treatment. Conclusion: When evaluating management for acute pain in the ED, a difference in VAS score of less than 20 mm without regard to the presence or absence of treatment is unlikely to signify a clinically meaningful reduction in pain severity. This study provides guidance to those who design and interpret clinical studies of the acute pain experience in the ED.

경남지역 건설폐기물 발생현황 및 폐콘크리트 재활용방안에 관한 연구

최도일(Choi, Do Il),어석홍(Eo, Seok Hong),이상준(Lee, Sang Jun) 한국콘크리트학회 2009 한국콘크리트학회 학술대회 논문집 Vol.2009 No.11

Roles of Plant Proteases in Pathogen Defense

백광현,최도일 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.4

The genomes of plants contain more than 600 genes encoding a diverse set of proteases and the subunits of proteasomes. These proteases and proteasomes consist of plant proteolytic systems, which are involved in various cellular metabolic processes. Plant proteolytic systems have been shown to have diverse roles in defense responses, such as execution of the attack on the invading organisms, participation in signaling cascades, and perception of the invaders. In order to provide a framework for illustrating the importance of proteolytic systems in plant defense, characteristics of non-proteasome proteases and the 26S proteasome are summarized. The involvement of caspase-like proteases, saspases, apoplastic proteases, and the 26S proteasome in pathogen defense suggests that plant proteolytic systems are essential for defense and further clarity on the roles of plant proteases in defense is challenging but fundamentally important to understand plant-microbe interactions.

Morphological Classification of Trichomes Associated with Possible Biotic Stress Resistance in the Genus Capsicum

김현정,최도일,서은영,김지현,정희진,강병철 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.1

Trichomes are specialized epidermal structure having the functions of physical and chemical block against biotic and abiotic stresses. Several studies on Capsicum species revealed that virus and herbivore resistance is associated with trichome-formation. However, there is no research on the structural characterization of trichomes developed on the epidermis of Capsicum spp. Thus, this study attempts to charaterize the trichome morphologies in 5 species of Capsicum using a Field Emission Scanning Electron Microscopy (FESEM). Six main trichome types were identified by their morphology under FESEM. Both glandular and non-glandular types of trichomes were developed on the epidermal tissues of Capsicum spp. The glandular trichome were further classified into type I, IV and VII according to their base, stalk length, and stalk. Non-glandular trichomes were also classified into type II, III, and V based on stalk cell number and norphology. Almost all the species in C. chinense and C. pubescens had glandular trichomes. To our knowledge, this is the first study on classification of trichomes in the genus Capsicum and,our results could provide basic informations for understanding the structure and function of trichomes on the epidermal differentiation and association with biotic stress tolerance.

Toward Functional Genomics of Plant-Pathogen Interactions:Isolation and Analysis of Defense-related Genes of Hot Pepper Expressed During Resistance Against Pathogen

이상협,최도일 한국식물병리학회 2002 Plant Pathology Journal Vol.18 No.2

To understand plant-pathogen interactions, a complete set of hot pepper genes differentially expressed against pathogen attack was isolated. As an initial step, hundreds of differentially expressed cDNAs were isolated from hot pepper leaves showing non-host resistance against bacterial plant pathogens (Xanthomonas campestris pv. glycines and Pseudomonas syringae pv. syringae) using differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique. Reverse Northern and Northern blot analyses revealed that 50% of those genes were differentially expressed in pepper leaves during non-host resistance response. Among them, independent genes without redundancy were micro-arrayed for further analysis. Random EST sequence database were also generated from various cDNA libraries including pepper tissue specific libraries and leaves showing non-host hypersensitive response against X. campestris pv. glycines. As a primary stage, thousands of cDNA clones were sequenced and EST data were analyzed. These clones are being spotted on glass slide to study the expression profiling. Results of this study may further broaden knowledge on plantpathogen interactions.