Characterization and Control of Vascellum curtisii (Berkeley)Kreisel Causing the Fairy Ring Arcs in the Golf Course in Korea

최대홍,이정한,김희규,Choi, Dae-Hong,Lee, Jung-Han,Kim, Hee-Kyu Turfgrass Society of Korea 2008 한국잔디학회지 Vol.22 No.2

골프코스에 농녹색으로 형성된 페어리링(arc)에서 군집으로 자라난 말불버섯목에 속하는 버섯을 발견하고, 분류학적 특성을 조사한 후 Vascellum curtisii로 동정하고, 친환경 방제법을 시도하였다. 버섯의 자실체 형태를 관찰한 결과 외피(exoperidium)상에 미세한 섬유상의 침상돌기(spine)가 빽빽하게 발달하였고, 촉감은 부드러웠으며 5-6개의 침상돌기(spine)가 끝이 한 곳으로 모아진 모양(Connivent)이 관찰되었다. 자실체는 점점 성숙하면서 spine은 점점 퇴화되어 길이가 짧아지고 약간의 갈색으로 변하면서 결국은 자실체 표면 위에 가루로 남게 된다. 흰색의 내피(endoperidium)는 회갈색으로 성숙된 후에는 중앙 정상부가 약간의 갈색을 띄기 시작하면서 구멍(apical pore)이 발달한다. 내부의 포자형성조직(gleba)은 초기에 다육질의 흰색이었다가, 성숙하면 내부에 올리브 갈색의 기본체(gleba)가 발달하고, 내부에 포자가 발달한다. 포자가 발달하지 않은 하부의 조직(subgleba or sterile babe)은 폭이 좁고, 조직내부는 챔버형이며 gleba와는 막(papery membrane)으로 구분되는 것이 특징이다. 포자는 올리브 갈색으로 크기는 $3-3.5{\mu}m$로 구형이며 돌기가 있으며 두꺼운 벽으로 되어있다. 또한 Lycoperdon 속과는 구분되는 특징으로 중요한 paracapillitium이 관찰되었는데 폭이 $8-9{\mu}m$로 넓고, KOH에 반응하지 않았으며 무색(hyaline)으로 그 벽은 얇다. 외관상 유사한 Vascellum pretense과 Lycoperdon pulcherrimum과 구분할 수 있는 특징을 상세하게 기술하고 비교하였다. 방제법은 농녹색의 ring arc를 직각으로 강하게 긁어서 thatch층이 노출되어 갈색을 띄게 될 때까지 균사매트를 파괴시킨 후 세제용액 500배액을 처리하고 1-2주후에 무처리와 비교한 결과 효과가 뛰어났다. We have found the clusters of tiny spiny puffball-like mushrooms growing gregariously in fairy ring (arcs) rimmed by a zone of darker green grass in the golf courses. Macroscopic as well as microscopic characters were examined for the morphology of fruiting body. Exoperidium is thin and densely spiny with minute fibrillae at early stage. The connivent spines were soft and quite persistent. In age, the fibrillae scrumble away with a powdery coating, which leaves white endoperidium becoming pale brown. It's interior was white and fleshy at first, but turns into an olive-colored dust as the gleba, the spore-producing tissue, develops to maturity and loaded with olive-brown spore mass. Then, distinct apical pore developed on the endoperidium. Rudimentary subgleba(sterile base) was narrow, chambered, delineated from the gleba by a membrane in young material. These characters suggested this fungus is a Vascellum, a member of the family Lycoperdaceae. The shapes of the spores were globose, echinulate, $3{\sim}3.5{\mu}m$ in diameter, thick-walled, and olive brown. Capillitial threads were $8-9{\mu}m$ wide, mostly colorless in KOH solution and thin-walled, which designated as "paracapillitium". This is an another character that distinguishes this mushroom from Lycoperdon spp. The spines developed on exoperidium were characteristically connivent; their apices joined together in a point, leaving a space below, which gives the appearance of vault to each group of usually 5 to 6 fibrillae. Based on the above characters, this fungus is identified as Vascellum curtisii (Berkeley). The characters distinguishable this from Lycoperdon pulcherrimum, and Vascellum pretense are discussed in detail. Control trial was also attempted. Strong vertical raking(SVR) followed by applying 500x detergent solution (Spark, Aekyung Co. Seoul) resulted in excellent control over any other treatments. In this plot, fruiting body was not developed throughout the end of mushroom growing season.

지상발표 : 간의 거대상 해면종 1예

최대홍,기춘석,박경남,김용일,이중달 대한소화기학회 1986 대한소화기학회 추계학술대회 Vol.1986 No.-

Characterization and Control of Vascellum curtisii (Berkeley)Kreisel Causing the Fairy Ring Arcs in the Golf Course in Korea

최대홍,이정한,김희규 한국잔디학회 2008 Weed & Turfgrass Science Vol.22 No.2

We have found the clusters of tiny spiny puffball-like mushrooms growing gregariously in fairy ring (arcs) rimmed by a zone of darker green grass in the golf courses. Macroscopic as well as microscopic characters were examined for the morphology of fruiting body. Exoperidium is thin and densely spiny with minute fibrillae at early stage. The connivent spines were soft and quite persistent. In age, the fibrillae scrumble away with a powdery coating, which leaves white endoperidium becoming pale brown. It’s interior was white and fleshy at first, but turns into an olive-colored dust as the gleba, the spore-producing tissue, develops to maturity and loaded with olive-brown spore mass. Then, distinct apical pore developed on the endoperidium. Rudimentary subgleba(sterile base) was narrow, chambered, delineated from the gleba by a membrane in young material. These characters suggested this fungus is a Vascellum, a member of the family Lycoperdaceae. The shapes of the spores were globose, echinulate, 3~3.5μm in diameter, thick-walled, and olive brown. Capillitial threads were 8-9μm wide, mostly colorless in KOH solution and thin-walled, which designated as “paracapillitium”. This is an another character that distinguishes this mushroom from Lycoperdon spp. The spines developed on exoperidium were characteristically connivent; their apices joined together in a point, leaving a space below, which gives the appearance of vault to each group of usually 5 to 6 fibrillae. Based on the above characters, this fungus is identified as Vascellum curtisii (Berkeley). The characters distinguishable this from Lycoperdon pulcherimum, and Vascellum pretense are discussed in detail. Control trial was also attempted. Strong vertical raking(SVR) followed by applying 500x detergent solution (Spark, Aekyung Co. Seoul) resulted in excellent control over any other treatments. In this plot, fruiting body was not developed throughout the end of mushroom growing season. We have found the clusters of tiny spiny puffball-like mushrooms growing gregariously in fairy ring (arcs) rimmed by a zone of darker green grass in the golf courses. Macroscopic as well as microscopic characters were examined for the morphology of fruiting body. Exoperidium is thin and densely spiny with minute fibrillae at early stage. The connivent spines were soft and quite persistent. In age, the fibrillae scrumble away with a powdery coating, which leaves white endoperidium becoming pale brown. It’s interior was white and fleshy at first, but turns into an olive-colored dust as the gleba, the spore-producing tissue, develops to maturity and loaded with olive-brown spore mass. Then, distinct apical pore developed on the endoperidium. Rudimentary subgleba(sterile base) was narrow, chambered, delineated from the gleba by a membrane in young material. These characters suggested this fungus is a Vascellum, a member of the family Lycoperdaceae. The shapes of the spores were globose, echinulate, 3~3.5μm in diameter, thick-walled, and olive brown. Capillitial threads were 8-9μm wide, mostly colorless in KOH solution and thin-walled, which designated as “paracapillitium”. This is an another character that distinguishes this mushroom from Lycoperdon spp. The spines developed on exoperidium were characteristically connivent; their apices joined together in a point, leaving a space below, which gives the appearance of vault to each group of usually 5 to 6 fibrillae. Based on the above characters, this fungus is identified as Vascellum curtisii (Berkeley). The characters distinguishable this from Lycoperdon pulcherimum, and Vascellum pretense are discussed in detail. Control trial was also attempted. Strong vertical raking(SVR) followed by applying 500x detergent solution (Spark, Aekyung Co. Seoul) resulted in excellent control over any other treatments. In this plot, fruiting body was not developed throughout the end of mushroom growing season.

골프코스의 이종잔디 조성과 혼입 현황

최대홍(Dae Hong Choi),박남일(Nam Il Park),최성환(Sung Hwan Choi),박기웅(Kee Woong Park),김진우(Jinwoo Kim),곽연식(Youn-Sig Kwak),이증주(Jeung Joo Lee) 韓國雜草學會 2012 Weed&Turfgrass Science Vol.32 No.3

한국잔디로 조성된 국내 골프코스에서 한지형잔디의 사용증가로 이종잔디의 혼입이 증가하고 있다. 본 연구에서는 전국 50곳의 골프코스들을 조사하여 이종잔디 식재와 혼입 현황을 파악하였다. 조사한 골프코스들 중에서 이종잔디가 1 종류 이상 혼입된 곳은 47곳으로 나타났으므로, 이종잔디의 혼입 문제는 전국 거의 모든 골프코스에서 발생하는 것으로 사료되었다. 이종잔디의 혼입은 한국잔디에서 크리핑벤트그래스와 켄터키블루그래스의 혼입 가능성이 있는 37곳 중 각각 25곳과 33곳, 켄터키블루그래스에서 크리핑벤트그래스의 혼입 가능성이 있는 47곳 중 43곳에서 발생하였다. 한편, 이종잔디의 혼입(한국잔디에 크리핑벤트그래스의 혼입, 한국잔디에 켄터키블루그래스의 혼입, 또는 켄터키블루그래스에 크리핑벤트그래스의 혼입)이 발생한 골프클럽 중 한 종류의 혼입이 나타난 곳은 14곳, 두 종류가 나타난 곳은 12곳, 세 종류 모두가 나타난 곳은 21곳이었다. The majority of turfgrass composition of golf courses are zoysiagrass, and mingle with cool-season turfgrasses have been increased. As result of mingling the species, invading problem has been raised. In this study, 50 golf courses nationwide were surveyed of grass composition and invading problem with hetero-species of turfgrasses. Among the courses, 47 golf courses have invading problem with more than one species at least. The invading problem has been issued most of golf course in nationwide. Thirty-seven courses composited with zoysiagrass; at 25 courses showed creeping bentgrass invading and at 33 courses were invaded by kentucky bluegrass. Among 47 kentucky bluegrass composition courses, 43 courses had creeping bentgrass invading problem. Invading problem within the grass species (creeping bentgrass in zoysiagrass, kentucky bluegrass in zoysiagrass, creeping bentgrass in kentucky bluegrass); 14, 12, and 21 courses were detected single species, two species, and three species invading problem, respectively.

골프장에서의 이종잔디 방제용 약제선발

최대홍(Choi, D. H.),최성환(S. H. Choi),안수정(J. S. Ahn),이증주(J. J. Lee) 한국잡초학회 2011 한국잡초학회 별책(학술대회 초록집) Vol.31 No.1

골프코스에서 혼입된 이종잔디의 방제

최대홍(Dae Hong Choi),박남일(Nam Il Park),최성환(Sung Hwan Choi),박기웅(Kee Woong Park),김진우(Jinwoo Kim),곽연식(Youn-Sig Kwak),이증주(Jeung Joo Lee) 韓國雜草學會 2012 Weed&Turfgrass Science Vol.32 No.3

본 연구는 골프코스에 혼입되어 문제가 되는 이종잔디를 제초제를 이용하여 효과적으로 방제하기 위해 수행되었다. 한국잔디에 혼입된 크리핑벤트그래스를 방제하기 위하여 asulam sodium 수용제와 trifloxysulfuron-sodium 입상수화제, 한국잔디에 혼입된 켄터키불루그래스를 방제하기 위하여 trifloxysulfuron-sodium 입상수화제, 켄터키불루그래스에 혼입된 크리핑벤트그래스를 방제하기 위하여 mesotrione 액상수화제를 각각 선발하였다. 크리핑벤트그래스에 대하여 asulam sodium 수용제와 trifloxysulfuron-sodium 입상수화제는 약제살포 70일 후 까지, mesotrione 액상수화제는 230일 후 까지, 켄터키불루그래스에 대하여 trifloxysulfuron-sodium 입상수화제는 110일 후 까지 90% 수준의 방제효과를 유지하였다. Asulam sodium 수용제와 trifloxysulfuron-sodium 입상수화제는 한국잔디에 대하여, mesotrione 액상수화제는 켄터키불루그래스에 대하여 각각 4배량의 처리에서도 약해가 미미하였다. 한국잔디에 혼입된 크리핑벤트그래스에 대하여 asulam sodium 수용제는 기준량 처리 40일 후에 80% 이상, trifloxysulfuron-sodium 입상수화제는 기준량 처리 30일 후에 85% 이상의 방제효과를 나타내었다. 한국잔디에 혼입된 켄터키불루그래스에 대하여 trifloxysulfuron-sodium 입상수화제는 기준량 처리 40일 후에 85%이상의 방제가를 나타내었다. 켄터키불루그래스에 혼입된 크리핑벤트그래스에 대하여 mesotrione 액상수화제는 기준량 처리 30일 후에 90%이상의 방제효과를 나타내었다. In this study, we attempted to control invaded hetero-species turfgrass in golf courses with herbicides. Asulam sodium SP and trifloxysulfuron-sodium WG (to control creeping bentgrass in zoysiagrass), trifloxysulfuron-sodium WG (to control kentucky bluegrass in zoysiagrass), and mesotrione SC (to control creeping bentgrass in kentucky bluegrass) were selected as efficient herbicides. To creeping bentgrass, asulam sodium SP and trifloxysulfuron-sodium WG until 70 days after application (DAA), mesotrione SC until 230 DAA and to kentucky bluegrass trifloxysulfuron-sodium WG until 110 DAA; the applications showed 90% herbicidal effect. At 4 X recommended application dose, asulam sodium SP, trifloxysulfuronsodium WG (to zoysiagrass) and mesotrione (to kentucky bluegrass) showed insignificant harmful effect. With recommended dose, asulam sodium SP showed 80% control effect of creeping bentgrass in zoysiagrass until 40 DAA and trifloxysulfuron-sodium WG demonstrated 85% control efficiency until 30 DAA. Trifloxysulfuron-sodium WG controlled 85% of kentucky bluegrass in zoysiagrass until 40 DAA. Mesotrione SC with recommended dose showed 95% control efficiency to creeping bentgrass in kentucky bluegrass.

Characterization and Control of Vascellum curtisii (Berkeley) Kreisel Causing the Fairy Ring Arcs in the Golf Course in Korea

Dae-Hong Choi(최대홍),Jung-Han Lee(이정한),Hee-Kyu Kim(김희규) 한국잡초학회·한국잔디학회 2008 Weed & Turfgrass Science Vol.22 No.2

골프코스에 농녹색으로 형성된 페어리링(arc)에서 군집으로 자라난 말불버섯목에 속하는 버섯을 발견하고, 분류학적 특성을 조사한 후 Vascellum curtisii로 동정하고, 친환경방제법을 시도하였다. 버섯의 자실체 형태를 관찰한 결과 외피(exoperidium)상에 미세한 섬유상의 침상돌기(spine)가 빽빽하게 발달하였고, 촉감은 부드러웠으며 5-6개의 침상돌기(spine)가 끝이 한 곳으로 모아진 모양(Connivent)이 관찰되었다. 자실체는 점점 성숙하면서 spine은 점점 퇴화되어 길이가 짧아지고 약간의 갈색으로 변하면서 결국은 자실체 표면 위에 가루로 남게 된다. 흰색의내피(endoperidium)는 회갈색으로 성숙된 후에는 중앙 정상부가 약간의 갈색을 띄기 시작하면서 구멍(apical pore)이 발달한다. 내부의 포자형성조직(gleba)은 초기에 다육질의 흰색이었다가, 성숙하면 내부에 올리브 갈색의 기본체(gleba)가 발달하고, 내부에 포자가 발달한다. 포자가 발달하지 않은 하부의 조직(subgleba or sterile base)은 폭이 좁고, 조직내부는 챔버형이며 gleba와는 막(papery membrane)으로 구분되는 것이 특징이다. 포자는 올리브 갈색으로 크기는 3-3.5㎛로 구형이며 돌기가 있으며 두꺼운 벽으로 되어있다. 또한 Lycopercdon 속과는 구분되는 특징으로 중요한 paracapillitium이 관찰되었는데 폭이 8-9㎛로 넓고, KOH에 반응하지 않았으며 무색(hyaline)으로 그 벽은 얇다. 외관상 유사한 Vascellum pratense과 Lycoperdon pulcherrimum과 구분할 수 있는 특징을 상세하게 기술하고 비교하였다. 방제법은 농녹색의 ring arc를 직각으로 강하게 긁어서 thatch층이 노출되어 갈색을 띄게 될 때까지 균사매트를 파괴시킨 후 세제용액 500배액을 처리하고 1-2주후에 무처리와 비교한 결과 효과가 뛰어났다. We have found the clusters of tiny spiny puffball-like mushrooms growing gregariously in fairy ring (arcs) rimmed by a zone of darker green grass in the golf courses. Macroscopic as well as microscopic characters were examined for the morphology of fruiting body. Exoperidium is thin and densely spiny with minute fibrillae at early stage. The connivent spines were soft and quite persistent. In age, the fibrillae scrumble away with a powdery coating, which leaves white endoperidium becoming pale brown. It's interior was white and fleshy at first, but turns into an olive-colored dust as the gleba, the spore-producing tissue, develops to maturity and loaded with olive-brown spore mass. Then, distinct apical pore developed on the endoperidium. Rudimentary subgleba(sterile base) was narrow, chambered, delineated from the gleba by a membrane in young material. These characters suggested this fungus is a Vascellum. a member of the family Lycoperdaceae. The shapes of the spores were globose, echinulate, 3-3.5㎛ in diameter, thick-walled, and olive brown. Capillitial threads were 8-9㎛ wide, mostly colorless in KOH solution and thin-walled, which designated as "paracapillitium". This is an another character that distinguishes this mushroom from Lycoperdon spp. The spines developed on exoperidium were characteristically connivent; their apices joined together in a point, leaving a space below, which gives the appearance of vault to each group of usually 5 to 6 fibrillae. Based on the above characters, this fungus is identified as Vascellum curtisii (Berkeley). The characters distinguishable this from Lycoperdon pulcherimum, and Vascellum pretense are discussed in detail. Control trial was also attempted. Strong vertical raking(SVR) followed by applying 500x detergent solution (Spark, Aekyung Co. Seoul) resulted in excellent control over any other treatments. In this plot, fruiting body was not developed throughout the end of mushroom growing season.

식물정유의 굼벵이에 대한 살충활성

이동곤,정영학,최대홍,최성환,추호렬,이동운 한국잔디학회 2015 Weed & Turfgrass Science Vol.4 No.2

White grub damages various crops, trees and they can also be one of the most serious pests of turf grass in golf courses. This study was conducted to determine the biocontrol of white grubs with 18 different kinds (anise, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, and turpentine) of plant essential oils in laboratory, green house and field. Anise oil (88.9%), linseed oil (100%), and teatree oil (88.9%) were highly effective among tested essential oils against 3rd instar of Bifurcanmala aulax in cap vial experiment. However, anise and linseed oils showed low mortality against 3rd instar of Popillia japonica in pot greenhouse experiment. Efficacy of anise, linseed oil, and tea-tree was also different depending on target white grub in field trials. Correlated mortality showed 32.6% only in tea-tree oil treatment against pupae of Adoretus tenuimaculatus however, correlated mortality of anise, linseed and tea-tree oil were 54.8, 51.6 and 56.5% respectively against 3rd instar of Exomala orientalis in the field trial in Adelscott Country Club in Hapcheon, Gyeongnam province. 굼벵이는 각종 작물과 나무에 피해를 줄 뿐만 아니라 특히 골프장 잔디에 피해를 주는 주요해충의 하나이다. 본 연구는 18종의 식물정유(anise oil, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, turpentine)를 이용하여 굼벵이의 환경친화적 방제 가능성을 알아보고자 실내와 온실, 야외에서 실험을 수행하였다. Cap vial실험에서 홈줄풍뎅이 3령충에 효과가 높은 정유는anise (88.9%)와 linseed oil (100%) 및 tea-tree oil (88.9%)이었다. 온실 pot에서 왜콩풍뎅이 3령충에 대한 anise와 linseed oil의 효과는 낮게 나타났다. 야외에서 anise와 linseed 및tea-tree oil의 효과도 대상 굼벵이의 종류에 따라 차이를 보였다. 주둥무늬차색풍뎅이 번데기에 대해서는 tea-tree oil 처리에서만 32.6%의 보정사충율을 보였으나 등얼룩풍뎅이3령충에 대해서는 anise와 linseed, tea-tree oil이 각각 54.8%, 51.6%, 56.5%의 보정사충율을 보였다.

지상발표 : α-1-Antitrypsin 결핍증을 동반한 간경변증 2예

기춘석,차호진,최대홍,윤인혁,이민호,이동후,강종명,박경남,조율희,백용균 대한소화기학회 1986 대한소화기학회 추계학술대회 Vol.1986 No.-

IgA 신병증의 임상적 고찰

박한철,박찬현,박문향,강종명,최대홍 대한내과학회 1987 대한내과학회지 Vol.32 No.2

We did clinicopathologic study in 43 patients with IgA nephropathy diagnosed during the period of January, 1985 through August, 1986 at Hanyang University Hospital. The results were as followings; 1) IgA nephropathy were diagnosed in 43 patients among 187 biopsied patients, the prevalence being 23%. 2) It is most common in the 3rd and 4th decades in this adult group. 71.8% of the patients were in these age aroup. The mean age of the patients was 31.4 years, 3) Male to female ratio was 1:l. 4) 53.8% of the patients presented with gross hematuria, and 57% of them had recurrent episodes. 5) The preceding history of the respiratory tract infection were noted in 56.4% of the patients. This history preceded in 85.7% of the patients with gross hematuria, while only 22.2% of the patients with microscopic hematuria had preceding URI history. 6) At the time of initial study, proteinuria was$lt; lg/24h in 56.4% of the patients, and it exceeded 3.5g/24h in 28.2% of them. 7) Patients with microscopic hematuria had more severe illness compared with those with gross hematuria. They had male preponderence, more severe proteinuria and higher blood pressure. 8) At the initial presentation, patients presenting with renal dysfunction have heavier proteinuria and higher blood pressure than those without renal dysfunction.