플라즈마 회전전극법에 의한 Al-Cu 합금 분말의 제조

최국선,김진영,이동희 연세대학교 대학원 1991 延世論叢 Vol.27 No.1

The production and characterization of Al-Cu alloy powders by the plasma- rotating-electrode process(PREP) was carried out to determine the influence of operating conditions(rotating speed, and plasma current) and alloy compositions on such particle characteristics as particle size distributon, shape, microstructure, and chemical composition. Each experimental condition showed a particle size range of 75∼353μm with mean sizes of 140∼160μm. The mean particle size of the powders changed with alloy compositions at the same rotating speed and plasma current. Particle shapes were mostly of the ligament type. Microstructures consisted mainly of dendrites, but also lamellar cell in the vicinity of eutectic composition. Al -20wt.% Cu powders of the same size, from even a single atomizing run, showed a wide variety of microstructure due to different thermal and nucleation history. Chemical compositions of alloy powders varied with particle size as a result of the interaction between high temperature plasma gas and material constituents of alloys during powder formation.

2원계 합금에 있어서 평면상 응고계면의 안정법에 관한 이론적 연구

최국선,한정호,김도훈,이동희 대한금속재료학회 1983 대한금속재료학회 학술대회 개요집 Vol.1983 No.1

파프리카 과실에 괴사반점을 일으키는 Pepper mild mottle virus의 병원형

최국선,최승국,조점덕,조인숙 한국식물병리학회 2013 식물병연구 Vol.19 No.2

Black necrotic spots were observed from the fruits of paprika that were cultivating in a vinylhouse. The casual agents of the symptom were identified as several isolates of Pepper mild mottle virus (PMMoV) by responses of indicator plants, electron microscopy, and RT-PCR analysis. Symptoms of the viral disease were mild mottle in the young leaves, necrotic spots on the fruits and the fruit apex of paprika, but the symptoms were not shown on the mature leaves. All of the PMMoV isolates were determined as P1.2.3 pathotypes from the biological responses on the chilli pepper lines used for discrimination of tobamovirus pathotypes. Pathogenicity of the PMMoV isolates was also confirmed using mechanical inoculation method to paprika seedlings. The coat protein (CP) genes of the PMMoV isolates were compared at the nucleotide and amino acid levels with the previously published PMMoV isolate. The isolates share 96 to 99% CP nucleotide identity among the isolates. The CP of P1.2-pathotype PMMoV-P2 presented Met at position 139, But the CPs of P1.2.3-pathotype PMMoVs from paprika showed Met to Asn substitution at the same position. This is the first report of identification of P1.2.3-pathotype PMMoV isolates from paprika in Korea. 시설재배단지에서 파프리카의 열매에 검은 괴저 반점증상이 발생하였다. 이 증상주를 전자현미경, 지표식물 검정 및 RT-PCR 분석 결과, 원인 바이러스로 PMMoV로동정되었다. 신엽에서 병징은 약한 모틀 증상, 과경과 과일에 검은 반점이 보였지만, 성숙한 잎에서는 증상이 나타나지 않았다. PMMoV의 분리주들에 대한 Tobamovirus pathotype(P)의 판별 고추계통에 접종한 결과, 모두 P1.2.3으로 확인되었다. 분리한 PMMoV를 건전 파프리카 유묘에 기계적 접종을 통하여 이 바이러스의 병원성을 증명하였다. PMMoV 분리주들의 외피단백질 유전자의 상동성은 96−99%였다. P1.2인 PMMoV-P2 분리주의 외피단백질 아미노산 배열 139번째가 Met인 반면, 파프리카 분리주 모두 이 위치가 Asn으로 확인되었다. 본 논문은 파프리카에서 PMMoV pathotype P1.2.3의 동정에 관한 우리나라 최초 보고이다.

고추약한모틀바이러스 병원형에 대한 파프리카 품종의 저항성 스크리닝

최국선,최승국,조인숙,권선정 한국식물병리학회 2014 식물병연구 Vol.20 No.4

The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causing necrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivars against PMMoV pathotypes, P1.2 and P1.2.3, using bioassay and genetic markers. L1, L3, and L4 genotypes expressing resistance to the pathotypes existed in those cultivars but L2 genotype did not. L4L4 in cvs. Easy andMagnifico, L4L3 in cvs. Scirocco and Orange glory F1, L4L1 in cv. Special F1, L3L3 in cvs. Fiesta, Piero and Derby,and L3L1 in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. wereFiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of L4 genotype showed systemicinfection when inoculated with PMMoV-P1.2.3. However, those cvs. despite the presence of L3 genotypeshowed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts wheninoculated with PMMoV-P1.2 The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causingnecrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivarsagainst PMMoV pathotypes, P1.2 and P1.2.3, using bioassay and genetic markers. L1, L3, and L4 genotypes expressingresistance to the pathotypes existed in those cultivars but L2 genotype did not. L4L4 in cvs. Easy andMagnifico, L4L3 in cvs. Scirocco and Orange glory F1, L4L1 in cv. Special F1, L3L3 in cvs. Fiesta, Piero and Derby,and L3L1 in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. wereFiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of L4 genotype showed systemicinfection when inoculated with PMMoV-P1.2.3. However, those cvs. despite the presence of L3 genotypeshowed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts wheninoculated with PMMoV-P1.2

Influence of Oxygen Concentration on the Hardness and Microstructure of Blended Elemental Ti/TiH2-6Al-4V Alloys (초)

최국선,허경호,( N. R. Munirathnam ),임재원,김원백 대한금속재료학회 ( 구 대한금속학회 ) 2008 대한금속재료학회 학술대회 개요집 Vol.2008 No.1

박과작물에 발생하는 2종의 Tobamovirus의 특성 및 예방

최국선 韓國施設園藝硏究會 2000 施設園藝硏究 Vol.13 No.1

고추에서 분리한 오이모자이크바이러스(CMV-GTN)의 특성과 고추 품종의 저항성 평가

최국선,권선정,최승국,조인숙,윤주연 한국식물병리학회 2015 식물병연구 Vol.21 No.2

Cucumber mosaic virus (CMV) is one of the most destructive viruses in chilli pepper. An isolate of CMV was obtained from the chilli pepper cv. Chungyang showing top necrosis symptom in 2013 and designated as CMV-GTN. CMV-GTN was compared with the well-characterized isolate, CMV-Ca-P1, by investigating their amino acid sequences of the coat protein (CP) and biological reactions in several host plants. The CP of CMV-Ca-P1 composed of 217 amino acids but that of CMV-GTN composed of 218 amino acids by including additional valine in the 57th amino acid position. Amino acid sequence similarity of the CP gene among CMV-GTN and other CMV isolates recorded in the GeneBank database ranged from 96% to 99%. CMV-GTN was selected as a representative isolate to screen the resistance pepper cultivars to CMV because it was highly pathogenic to tomatoes and peppers upon biological assays. The virulence of CMV-GTN was tested on 135 pepper cultivars which has been bred in Korea and compared with that of CMV-Ca-P1. Only the cv. Premium was resistant and three cvs. Hot star, Kaiser, and Good choice were moderately resistant to CMV-GTN, whereas two cvs. Baerotta and Kaiser were resistant to CMV-Ca-P1. 오이모자이크바이러스(CMV)는 고추에 심한 피해를 주는 바이러스이다. 2013년 고추 품종 청양의 선단부위에 괴저증상이 나타난 식물체로부터 CMV를 순수 분리하였고 이 분리주를CMV-GTN으로 명명하였다. CMV-GTN과 기존에 특성이 잘 알려진 CMV-Ca-P1을 대상으로 외피단백질의 아미노산 서열과 여러 기주식물에서 생물적 반응을 비교하였다. CMV-Ca-P1의 외피단백질 아미노산은 217개로 구성되어 있었으나, CMV-GTN 은 아미노산 서열 57번째에 발린이 추가된 218개로 구성되어있었다. CMV-GTN과 GeneBank에 등록된 다양한 CMV 분리주들의 외피단백질유전자 유사성은 96-99%였다. 생물 검정에서토마토와 고추에서 병원성이 강하게 발현되는 CMV-GTN을 고추 품종에 대한 저항성 스크린을 위하여 선발하였다. 고추 시판품종 135종에 대하여 CMV-GTN으로 검정하였고 CMV-Ca-P1과저항성 반응을 비교하였다. 그 결과 CMV-GTN에 대하여 저항성반응을 표현한 품종은 프레미엄, 중도 저항성 품종은 핫스타, 카이저, 굿쵸이스였다. 한편 CMV-Ca-P1에 대한 저항성 품종은베로따와 카이저였다.

우리나라에서 발생하는 딸기 바이러스병(2007-2008)

최국선,이진아,조점덕,정봉남,조인숙,김정수 한국식물병리학회 2009 식물병연구 Vol.15 No.1

Virus disease surveys of strawberries cultivated and preserved as germplasm resources in Korea was conducted during 2007-2008. Virus detection was conducted by RT-PCR using total RNAs extracted from strawberry samples. We detected the infection with Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry vein banding virus (SVBV) and Strawberry pallidosis associated virus (SPaV) while no infection with Strawberry crinkle virus (SCV), Strawberry necrotic shock virus (SNSV), Strawberry latent ring spot virus (SLRSV) and Arabis mosaic virus (ArMV) was observed. The infection rate of virus disease on 4 cultivars including Seolhyang, Maehyang, Gumhyang, and Dahong, bred in Korea, was 0.1, 1.9, 0, and 0%, respectively. Surprisingly, however, cultivar Red Peal introduced from Japan in 1997 revealed 48.3% virus infection rate. SMYEV, SMoV and SPaV were also identified in strawberries growing in the farm fields of Korea. In the field, however, SMYEV was the most predominant virus (97.4%) among those 3 identified viruses. SVBV was detected only in strawberry kept as a germplasm. Virus disease surveys of strawberries cultivated and preserved as germplasm resources in Korea was conducted during 2007-2008. Virus detection was conducted by RT-PCR using total RNAs extracted from strawberry samples. We detected the infection with Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry vein banding virus (SVBV) and Strawberry pallidosis associated virus (SPaV) while no infection with Strawberry crinkle virus (SCV), Strawberry necrotic shock virus (SNSV), Strawberry latent ring spot virus (SLRSV) and Arabis mosaic virus (ArMV) was observed. The infection rate of virus disease on 4 cultivars including Seolhyang, Maehyang, Gumhyang, and Dahong, bred in Korea, was 0.1, 1.9, 0, and 0%, respectively. Surprisingly, however, cultivar Red Peal introduced from Japan in 1997 revealed 48.3% virus infection rate. SMYEV, SMoV and SPaV were also identified in strawberries growing in the farm fields of Korea. In the field, however, SMYEV was the most predominant virus (97.4%) among those 3 identified viruses. SVBV was detected only in strawberry kept as a germplasm.

전자빔 drip 용해횟수가 Mo 잉고트 특성에 미치는 영향

최국선,이강인,이동희 ( Good Sun Choi,Kang In Rhee,Dong Hi Lee ) 한국주조공학회 1995 한국주조공학회지 Vol.15 No.3

N/A Molybdenum ingot of 50mm in diameter were obtained from sintered Mo bars by EB drip melting technique. Macroscopic observation of EB remelted ingot indicates that coarse and columnar grains grow in the direction parallel to ingot pulling direction. This can be explained by slow solidification (3㎚/min), large temperature gradient and heat flow to this direction. The orientation of columnar structure was found to be <110>, <200> and <211> by the analysis of X-ray diffraction patterns. The contents of typical metallic impurities in Mo sintered bar are 1.2ppm Cr, 3ppm Fe, 44ppm Zr, 150ppm W. Most of metallic impurities were reduced below the order of ppm except zirconium and tungsten by the selective evaporation. In the removal of nonmetallic impurities, oxygen and carbon impurities were lowered from 120 to 6ppm and from 157 to 106ppm, respectively, after first melting. Although the purification effect was not significant with the number of remelting, Vickers hardness was reduced from 217 to 195 and 184 in sequence with increasing the number of remelting.

TiAl 금속간화합물 분말의 미세구조에 관한 연구

최국선,이동희 대한금속재료학회 1991 대한금속재료학회 학술대회 개요집 Vol.1991 No.1