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Principle of Emulsion PCR and Its Applications in Biotechnology
채창훈 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.4
Emulsion polymerase chain reaction (PCR) is performed on compartmentalized DNA, allowing a large number of PCR reactions to be carried out in parallel. Emulsion PCR has unique advantages in DNA amplification. It can be applied in many molecular biological assays, especially those requiring highly sensitive and specific DNA amplification. This review discusses the principle of emulsion PCR and its applications in biotechnology. Related technologies are also discussed.
채창훈,Jinhyung Park 한국축산학회 2023 한국축산학회지 Vol.65 No.1
Climate change has worsened droughts and floods, and created conditions more likely to lead to pathogen contamination of surface water and groundwater. Thus, there is a growing need to disinfect livestock water. Ultraviolet (UV) irradiation is widely accepted as an appropriate method for disinfecting livestock water, as it does not produce hazardous chemical compounds and kills pathogens. However, UV-based disinfection inevitably consumes electricity, so it is necessary to improve UV disinfection effectiveness. Aluminum-based reflective nanolens arrays that enhanced the effectiveness of a continuous-flow UV water disinfection system were developed using electrochemical and chemical processes, including electropolishing and two-step anodization. A continuous UV disinfection system was custom designed and the parts were produced using a three-dimensional printer. Electropolished aluminum was anodized at 40 and 80 V in 0.3 M oxalic acid, at 120 and 160 V in 1.0 M phosphoric acid, and at 200 and 240 V in 1.5 M citric acid. The average nanolens diameters (D) of the aluminum-based reflective nanolens arrays prepared using 40, 80, 120, 160, 200, and 240 V anodization were 95.44, 160.98, 226.64, 309.90, 296.32, and 339.68 nm, respectively. Simple UV reflection behind irradiated water disinfected Escherichia coli O157:H7 in water more than did the non-reflective control. UV reflection and focusing behind irradiated water using an aluminum-based reflective nanolens array disinfected E. coli O157:H7 more than did simple UV reflection. Such enhancement of the UV disinfection effectiveness was significantly effective when a nanolens array with D 226.64 nm, close to the wavelength of the irradiated UV (254 nm), was used.
채창훈,장해진,오세욱 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.1
Shigella sonnei shares many physiological aspects with Escherichia coli; thus, so far no culture-based method has been developed to detect and quantify S. sonnei separately from E. coli. Therefore, little information is available for the growth characteristics of S. sonnei in food. This study aimed to address a systematic scheme to quantify S. sonnei in beef separately from E. coli using quantitative real-time polymerase chain reaction (qRTPCR) and subsequently predict its growth characteristics. The use of S. sonnei-specific primers in qRT-PCR allowed to obtain growth curves of S. sonnei in beef at different temperatures, and the fitting of curves into a modified Gompertz model let us analyze the growth characteristics such as the lag time, maximum growth rate, and maximum quantity of S. sonnei in beef at different temperatures. A systematic scheme for RT-PCR-based quantification and a predictive modeling described in this study may be a useful means to analyze S. sonnei growth in food.
Emulsion PCR to Improve Sensitivity of PCR-based E. coli O157:H7 ATCC 35150 Detection
채창훈,오세욱 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
Conventional PCR (cPCR) with 38 thermal cycles (cPCR38cycles) failed to amplify 3 copies of Escherichia coli O157:H7 DNA at the presence of 3.28×106 copies of Salmonella Typhimurium DNA, but emulsion PCR (ePCR) with 20 thermal cycles and a subsequent conventional PCR with 38 thermal cycles (ePCR20cycles-sPCR38cycles) amplified them. Partitioning individual E. coli O157:H7 DNA with emulsion droplet improved the specificity of PCR and let 3 copies of E. coli O157:H7 DNA to be amplified specifically at the presence of 3.28×106 copies of S. Typhimurium DNA. Application of ePCR improved the sensitivity of PCR-based pathogen detection and enabled the detection of 3 copies of E. coli O157:H7 DNA.
Electrochemical impedimetric biosensors for food safety
채창훈,오세욱 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.7
Electrochemical impedimetric biosensors (EIBs)have a simple structure and can be used to rapidly andsensitively detect and measure hazards in food. EIBs detectand measure target molecules by transducing biochemicalreactions on their surface to electrical signal outputsresponding to a sinusoidal electrical signal input. Due totheir structural simplicity and analytical sensitivity, EIBsare regarded as the most potent method of food hazardmonitoring that can be implemented in the food supplychain. This paper discusses the theoretical background,structure, and construction of EIB and its applications infood safety.
Roles of Milk Fat Globule Membrane on Fat Digestion and Infant Nutrition
채창훈,오세종,임지영 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.3
Milk fats are present as globules emulsified in the aqueous phase of milk and stabilized by a delicate membrane architecture called milk fat globule membrane (MFGM). The unique structure and composition of the MFGM play an important role in fat digestion and the metabolic programming of neonates. The objective of this review is to compare the structure, composition, and physicochemical characteristics of fat globules in human milk, bovine milk, and infant formula. It provides an overview of the fat digestion process and enzymes in healthy infants, and describes the possible roles of the MFGM in association with factors affecting fat digestion. Lastly, the health benefits of the MFGM on infant nutrition and future perspectives are discussed with a focus on brain development, metabolic response, and gut health.