V_2O_5-AS_2O_3-Na_2O-BaO系 유리반도체의 Switching현상과 IR 흡수 Spectra에 관한 연구

채인경 숙명여자대학교 대학원원우회 1990 원우논총 Vol.8 No.-

Current-Voltage characteristic were investigated as a funtion of Temperature from 50℃ to 150℃ for V_2O_5-As_2O_3-Na_2O-BaO glasses having 51mol %, 65mol %, 75mol % V_2O_5. Threshold voltage of switching effect decrease with increasing temperature and V_2O_5mol %. It is concluded that the switching effect may be associated with a phase transition from a disordered glasses state to an ordered devitrified state due to self-heating effects. Absorption bands of V_2O_5-As_2O_3-Na_2O-BaO glasses have been studied by Infrared spectroscopy(400-4000㎝^1). Absorption bands shift to long wavelength as V_2O_5mol% and BaOmol% increase.

Effect of Rosemarinus officinalis L. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

채인경,유미희,임남경,정영태,이진호,천경수,이인선 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10

Atherosclerosis is a chronic and progressive inflammatory disease. Novel anti-inflammatory therapies may have promise as treatment strategies for cardiovascular risk reduction. Rosemary (Rosemarinus officinalis L.) has been used in folk medicine to treat headaches, epilepsy, poor circulation, and many other ailments. It was found that rosemary could act as a stimulant and mild analgesic and could reduce inflammation. However, the mechanisms underlying the anti-inflammatory and antiatherosclerotic effects of rosemary need more study. This study investigated effects of the rosemary components, carnosic acid (CA), and carnosol (CAR), on cell migration. Monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) were determined by Western blot and gelatin zymography, respectively, in RAW 264.7 macrophages and vascular smooth muscle cells (VSMCs). VSMC migration was assessed by a Matrigel migration assay. Active compounds of rosemary extracts were also analyzed using a reversed-phase high-performance liquid chromatography. MMP-9 and MCP-1 activities were markedly diminished with methanol extract (RM), n-hexane fraction (RH), and CA in RAW 264.7 cells. RM, RH, CA, and CAR suppressed tumor necrosis factor-alpha–induced VSMC migration by inhibiting MMP-9 expression. Chromatograms of RM- and RH-containing CA and CAR revealed higher CA contents of RM (9.4%, 93.85 μg/mg dry wt.) and, especially, RH (18.4%, 184.00 μg/mg dry wt.), which were appreciably elevated compared with the similar CAR content in RM and RH (3.7%, 37.30 μg/mg dry wt.; and 2.5%, 25.05 μg/mg dry wt., respectively). Rosemary, especially its CA component, has potential antiatherosclerosis effects related to cell migration.

주안역 그린공간 만들기 아이디어 제안

채인경,김예지 한국환경정책학회 2011 한국환경정책학회 학술대회논문집 Vol.2011 No.10

국내 시판되는 허브류의 항산화 및 항균효과 검색

채인경(In-Gyeong Chae),김현정(Hyun-Jeong Kim),유미희(Mi-Hee Yu),김혁일(Hyuk-Il Kim),이인선(In-Seon Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.10

총 폴리페놀 함량은 로즈마리에서 가장 높은 폴리페놀 함량을 보였고, 구절초, 라벤더, 레몬그라스도 72~80 ㎍/㎎의 비교적 높은 폴리페놀 함량을 보였다. 반면 카렌듈라와 목향은 다른 허브류보다 20~40% 정도의 낮은 폴리페놀의 함량을 보였다. 그리고 총 플라보노이드 함량에서는 레몬그라스, 로즈마리, 구절초, 카렌듈라, 라벤더, 목향 순으로 높은 함량을 나타냈다. 로즈마리, 라벤더, 구절초, 레몬그라스는 최고농도에서 90% 이상의 높은 DPPH 소거능이 있었고, IC??값은 로즈마리 6.23±0.58 ㎍/mL, 라벤더 18.29±0.68 ㎍/mL, 구절초 20.33±1.04 ㎍/mL, 레몬그라스 22.38±0.77 ㎍/mL, 카렌듈라 60.03±4.01 ㎍/mL, 목향 65.64±2.64 ㎍/mL이었다. 이들 허브 추출물들의 항균 활성을 살펴보면, S. aureus 균주는 최고농도 100 ㎎/mL에서 구절초 14.5 ㎜, 로즈마리 15 ㎜로 가장 뛰어난 항균활성을 나타내었고, E. coli O157:H7 균주는 최고농도 100 ㎎/mL에서 구절초 16㎜, 로즈마리 17 ㎜로 우수한 항균효과를 나타냈다. 따라서 허브류 중에서 로즈마리 추출물이 가장 높은 총 폴리페놀의 함량을 보였고, 총 플라보노이드의 함량은 레몬그라스에서 가장 높았다. 또한 S. aureus와 E. coli O157:H7 2종류의균에 대한 항균 효과는 로즈마리와 구절초에서 가장 뛰어난 효과를 확인하였다. In this study, we investigated the antioxidant and antibacterial activity of methanol extracts from 6 plants which were Chrysanthemum zawadskii Herb. var. latilobum (Maxim.) Kitamura (Gu-jeol-cho), Lavandula spica L. (Lavender), Rosmainus offcinals L. (Rosemary), Cymbopogon citrates (Lemongrass), Saussureae radix Mok-hyang), Calendular officinalis L. (Calendular). Antioxidative effects of herbal extracts were measured by polyphenols, flavonoids contents and DPPH radical scavenging activity assay. We also evaluated the antibacterial activity against Staphylococcus aureus and Escherichia coli O157:H7. Methanol extracts from Gu-jeol-cho, lavender, rosemary and lemongrass showed high polyphenols contents as well as strong DPPH scavenging activity. In particular, rosemary extract contained highest polyphenol levels as 126.69±2.62 ㎍/㎎ compared to other herbs. As for DPPH radical scavenging activities, IC?? values of rosemary extracts were 6.23±0.58 ㎍/mL. The rosemary extracts also showed higher antibacterial effects against S. aureus and E. coli O157:H7. These results indicate that rosemary could be used as natural antioxidant and antibacterial agents.

가자, 라벤더, 강향의 항염증 및 항산화 활성 검색

In Gyeong Chae(채인경),Mi Hee Yu(유미희),Hyuk Il Kim(김혁일),In Seon Lee(이인선) 한국생명과학회 2011 생명과학회지 Vol.21 No.4

염증반응의 중요한 작용인자로 알려진 nitric oxide (NO) 저해율에 대한 24종 천연물 추출물의 효과를 25 ㎍/㎖의 농도에서 검색해 본 결과, 다른 추출물에 비해 가자, 라벤더, 강향이 우수한 효과를 나타냈다. 하지만 강향은 세포 생존율이 52.67±5.66%인 것으로 보아 세포독성으로 인해 NO 저해율이 높게 나타난 것으로 보여 지므로 농도를 낮춰 다음 실험을 진행하였다. 농도별 NO생성능을 측정해본 결과. 최고농도인 100 ㎍/㎖에서 가자와 라벤더는 NO 생성량이 20-30%로 감소하였으며, 강향은 10 ㎍/㎖에서 NO 생성량이 약 30%로 다른 시료에 비해 낮은 농도에서 높은 저해활성을 나타내었다. RAW 264.7 세포주에 LPS와 각각의 시료를 처리하여 염증을 유발한 후 염증인자인 NO의 생성, 그리고 proinflammatory cytokines인 TNF-α의 생성량을 측정한 결과, 강향 추출물은 낮은 농도에서 높은 저해활성을 나타내, LPS로 유도된 염증 인자들을 효과적으로 감소시키는 것으로 나타났다. 각각의 총 폴리페놀 함량은 가자 616 ㎍/㎎, 라벤더 73.98 ㎍/㎎, 강향 161.82 ㎍/㎎으로 세 가지 추출물 모두 높은 함량을 지내고 있었으며, 특히 가자 추출물의 폴리페놀 함량은 매우 높았다. 플라보노이드 함량은 가자 96.13 ㎍/㎎, 라벤더 11.77 ㎍/㎎, 강향 41.21 ㎍/㎎으로 또한 가자 추출물이 가장 높은 함량을 나타내었다. 각각의 RC50값은 가자 2.09 ㎍/㎖, 라벤더 17.25 ㎍/㎖, 강향 6.31 ㎍/㎖로 나타났으며 세 가지 시료 모두 낮은 RC50값으로 DPPH radical 소거능이 우수하였다. 특히 가자는 천연항산화제인 ascorbic acid (1.57 ㎍/㎖)와 비슷한 RC50값을 나타내 뛰어난 효과로 보여 진다. This study was performed to evaluate the anti-inflammatory and antioxidant activities of methanol extract from natural products. Cell viability was determined by MTT assay. The production of NO and TNF-α were measured by Griess assay and enzyme-linked immunosorbent assay (ELISA). In order to effectively screen for anti-inflammatory agents, we first examined the inhibitory effects of 24 natural products on the production of lipopolysaccharide (LPS)-induced nitric oxide (NO) in RAW 264.7 cells. Three extracts of Terminalia chebulaRetz., Lavandula spicaL., and Dalbergia odoriferaT. significantly inhibited NO production. The three extracts significantly decreased production of NO in a dose-dependent manner. Terminalia chebulaRetz. decreased TNF-α production. Antioxidative effects of the three extracts were measured based on polyphenol and flavonoid contents and DPPH radical scavenging activity assay. The three extracts showed high polyphenol contents as well as strong DPPH scavenging activities. In particular, Terminalia chebulaRetz. contained the highest polyphenol and flavonoid levels of 616 and 96 ㎍/㎎, respectively, compared to Lavandula spica L. and Dalbergia odoriferaT. As DPPH radical scavensing activities, RC50 values of Terminalia chebulaRetz. were 2.09 ㎍/㎖.

V_2O_5-As_2O_3-Na_2O-BaO系 유리半導體의 電氣的 特質에 관한 硏究

박애주,채인경 淑明女子大學校 1990 論文集 Vol.30 No.-

Glasses of V_2O_5-As_2O_3-Na_3O-BaO system varying concentration ratio of the compotents have been prepared and their electric propperties, such as, dc electric resistivity, dielectric constant and dielectric loss have been measured. Electric resistivity of the glasses are measured at the break temperature between 27℃ to 180℃ and are found to be decreasing as temperature and concentration of V_2O_5 increase. Plots of In ρ vs 1/T show two straight lines breaking at the break temperature and activation energies are calculated from these lines in the range of 0.322 eV∼0.505 eV for the low temperature region and 0.437 eV∼0.638 eV for the high temperature region, respectively. Dielectric constants of the glasses are found to be exponentially increasing as frequencies decrease. At a given frequency, the dielectric constant is larger for the higher temperature. The Dielectric losses of the glasses show turning points at a certain frequency. The turning points are shifted to the higher frequency when temperatures are increased. Dielectric characteristics of the glasses are observed to satisfy the Debye theory.

Effects of Fermented Red Ginseng Extracts on Hyperglycemia in Streptozotocin-induced Diabetic Rats

김현정,채인경,이성규,정현진,이은주,이인선 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.2

Fermented red ginseng (FRG) was prepared by inoculating 0.1% Lactobacillus fermentum NUC-C1 and fermenting them at 40°C for 12 hours. The ginsenoside contents of FRG were increased compared with those of red ginseng (RG). Moreover,the levels of the ginsenosides Rg2, Rg3, and Rh2 in FRG increased significantly. In an oral glucose tolerance test (OGTT),blood glucose levels were lower in animals fed with RG and FRG extracts than in normal controls. In particular, FRG extracts in OGTT were superior to RG extracts. The antidiabetic effects of FRG in streptozotocin (STZ)-induced diabetic rats were investigated. Rats were divided into four groups: normal control, diabetes mellitus (DM), FRG administered at 100 mg/kg,and FRG administered at 200 mg/kg groups. FRG extracts were orally administered to each treatment group for 3 weeks, and blood glucose, insulin, and lipid levels of each group were determined. Orally administered FRG extracts significantly reduced blood glucose levels and increased plasma insulin levels in diabetic rats. Additionally, the activities of disaccharidases, including sucrase, lactase, and maltase, were decreased significantly in the FRG groups. FRG groups also had reduced triglyceride and total cholesterol levels, compared with the DM group. These results suggest that FRG may have antidiabetic effects in STZ-induced diabetic rats.

Fraxetin Induces Heme Oxygenase-1 Expression by Activation of Akt/Nrf2 or AMP-activated Protein Kinase /Nrf2 Pathway in HaCaT Cells

Juthika Kundu,채인경,천경수 대한암예방학회 2016 Journal of cancer prevention Vol.21 No.3

Background: Fraxetin (7,8-dihydroxy-6-methoxy coumarin), a coumarin derivative, has been reported to possess antioxidative, antiinflammatory and neuroprotective effects. A number of recent observations suggest that the induction of heme oxygenase-1 (HO-1) inhibits inflammation and tumorigenesis. In the present study, we determined the effect of fraxetin on HO-1 expression in HaCaT human keratinocytes and investigated its underlying molecular mechanisms. Methods: Reverse transcriptase-PCR and Western blot analysis were performed to detect HO-1 mRNA and protein expression, respectively. Cell viability was measured by the MTS test. The induction of intracellular reactive oxygen species (ROS) by fraxetin was evaluated by 2‘,7‘-dichlorofluorescin diacetate staining. Results: Fraxetin upregulated mRNA and protein expression of HO-1. Incubation with fraxetin induced the localization of nuclear factor-erythroid-2-related factor-2 (Nrf2) in the nucleus and increased the antioxidant response element-reporter gene activity. Fraxetin also induced the phosphorylation of Akt and AMP-activated protein kinase (AMPK)α and diminished the expression of phosphatase and tensin homolog, a negative regulator of Akt. Pharmacological inhibition of Akt and AMPKα abrogated fraxetin-induced expression of HO-1 and nuclear localization of Nrf2. Furthermore, fraxetin generated ROS in a concentration-dependent manner. Conclusions: Fraxetin induces HO-1 expression through activation of Akt/Nrf2 or AMPKα/Nrf2 pathway in HaCaT cells.

Antioxidant Effects of Fermented Red Ginseng Extracts in Streptozotocin-Induced Diabetic Rats

김현정,이성규,채인경,김미진,임남경,유미희,이은주,이인선 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.2

The antioxidant activities of fermented red ginseng (FRG) were investigated in vitro and in vivo. The contents of total polyphenol and total flavonoid in FRG extracts were 17.01±2.00 μg/mg and 18.42±3.97 μg/mg, respectively. These extracts were capable of directly scavenging α, α-diphenyl-picrylhydrazyl free radicals. The antioxidative effects of the FRG extracts in streptozotocin (STZ)-induced diabetic rats were also investigated. The activities of plasma alanine transaminase, aspartate transaminase, and γ-glutamyltransferase were significantly decreased by extract administration as compared to an STZ control group. Hepatic glutathione content depleted by STZ treatment was significantly increased by treatment of the FRG extracts, but the elevation of lipid peroxide content induced by STZ was significantly decreased by the extracts. Activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase decreased after STZ-treatment were recovered by the treatment of the FRG extracts. These results indicate that FRG extracts have antioxidative effets in STZ-induced diabetic rats.

개다래 초임계 박추출물이 염증 및 동맥경화에 미치는 영향

유미희(Mi Hee Yu),채인경(In Gyeong Chae),최준혁(Jun Hyeok Choi),임효권(Hyo Gwon Im),최희돈(Hee Don Choi),양선아(Seun Ah Yang),이진호(Jin Ho Lee),이인선(In-Seon Lee) 한국식품과학회 2010 한국식품과학회지 Vol.42 No.4

개다래(Actinidia polyama Max.)는 복통, 류마티스 관절염, 뇌졸중 치료에 사용되었으며, 최근 소염, 진통, 통풍에 효과가 있다고 알려져 있으나 특유의 강한 향과 맛을 지니고 있어 기능성 식품이나 치료용 약물을 개발하고자 할 때 많은 제약이 따른다. 따라서 개다래를 초임계 추출하여 향과 맛을 개선하고, 본 연구에서는 초임계추출 후 남은 부산물을 이용하여 (박)추출물을 제조하고 항염증 및 항동맥경화 활성을 조사하였다. 개다래 주정 추출물 및 초임계 박 추출물의 NO 소거활성을 비교한 결과, 개다래박 11 추출물 10, 100 ㎍/㎖의 농도에서 36.63, 79.58%의 NO 소거활성을 보여 주정 추출물(4.61, 19.00%)에 비해 훨씬 높은 NO 소거활성을 보였다. 또한 RAW 264.7 세포주에 개다래 초임계 박 11 추출물을 처리하고, LPS를 처리하여 염증을 유발한 후염증인자인 PGE₂의 생성, 그리고 proinflammatory cytokines인 TNF-α의 생성량을 측정한 결과, 개다래 박 11 추출물은 LPS로 유도된 염증 인자들을 효과적으로 감소시키는 것으로 나타났다. 또한 박 11 추출물의 MMP-9 활성에 미치는 영향을 알아보기 위해 zymography를 실시한 결과, 추출물 50, 100 ㎍/㎖의 농도에서 MMP-9의 활성이 효과적으로 감소됨을 확인하였으며, MMP-9의 단백질 발현도 억제됨을 확인하였다. 결론적으로 개다래 박11 추출물은 LPS로 유도된 염증 인자들을 효과적으로 감소시켰으며, TNF-α에 의해 증가된 MMP-9의 활성을 억제하는 것으로 나타나 동맥경화를 비롯한 고혈압, 암, 당뇨, 관절염 등의 만성염증성 질환의 예방과 치료에 효과적으로 적용할 수 있을 것으로 생각된다. The fruit of Actinidia polygama, Mock-chun-ryo in Korea, has been used as traditional medicine for abdominal pain, rheumatic arthritis, and stroke. In a previous study, the ethanol extract of A. polygama Max. showed antiinflammatory activity in RAW 264.7 cells. In this study, we investigated the anti-inflammatory and anti-atherosclerosis effects of supercritical fluid marc extracts from A. polygama Max. Anti-inflammatory extracts were produced from supercritical fluid extraction of the silver vine under the following conditions; pressure, 1,500-4,500 psi, temperature 35-55℃ and extraction time 1-2 hr. To evaluate the anti-inflammatory and anti-atherosclerotic effects of the extracts, we studied nitric oxide (NO), prostaglandin E₂ (PGE₂), and tumor necrosis factor-alpha (TNF-α) levels in RAW 264.7 cells and MMP-9 activity in human aortic smooth muscle cells (HASMC). The Marc 11 extract inhibited the production of NO, PGE₂, and TNF-α by lipopolysaccharide in RAW 264.7 cells. Moreover, the marc 11 extract inhibited TNF-α-induced MMP-9 activity in HASMC. These results indicate that the Marc 11 extract of A. polygama Max. has the potential for use as an anti-atherosclerosis agent.