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New record of three oxytrichid ciliates (Ciliophora: Oxytrichidae) from South Korea
채규석,민기식 국립생물자원관 2022 Journal of species research Vol.11 No.4
Three oxytrichid ciliates, Oxytricha lithofera Foissner, 2016, Pleurotricha curdsi (Shi et al., 2002) Gupta et al., 2003 and Sterkiella tetracirrata Kumar et al., 2015, were isolated from soils and confirmed as new to South Korea. Oxytricha lithofera was identified based on lithosomes, cortical granules, 21-33 adoral zone membranelles, one left (14-21 cirri) and one right (15-18) marginal rows and 5 transverse cirri. Pleurotricha curdsi was identified based on the possession of 2 macronuclear nodules, 2-3 micronuclei, 46-53 adoral zone membranelles, 3 frontal cirri, 5 frontoventral cirri, 5-7 postoral ventral cirri, 2-3 right marginal rows and 5 transverse cirri. Sterkiella tetracirrata was identified with respect 4 macronuclear nodules, 3-6 micronuclei, 25-40 adoral zone membranelles, 3 frontal cirri, 3 postoral ventral cirri, 2 pretransverse cirri, one left (21-30 cirri) and one right (24-30) marginal row and 4 transverse cirri. On the basis of 18S rDNA sequence analyses, we describe the phylogenetic positions of the three species.
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채규석,정종우,이원제,민기식 국립생물자원관 2022 Journal of species research Vol.11 No.4
Through the ‘Discovery of Korean Indigenous Species’ project hosted by the National Institute of Biological Resources (NIBR), new or unrecorded species of various protozoans inhabiting the Korean Peninsula have been discovered. Samples were collected from marine, freshwater, and soil habitats in South Korea and all species were identified and reviewed by experts working on the respective taxonomic group. This study includes 17 unrecorded Korean protozoan species discovered through this project, which belong to four phyla: Amoebozoa Lühe, 1913, Cercozoa Cavalier-Smith, 1998, Euglenozoa Cavalier-Smith, 1981, and Ciliophora Doflein, 1901. Among them, three families (Rhogostomidae Dumack et al., 2017, Parauronematidae Small and Lynn, 1985, and Cyclidiidae Ehrenberg, 1838) and three genera (Rhogostoma Belar, 1921, Parauronema Thompson, 1967, and Cyclidium Müller, 1773) were reported for the first time in Korea. Unlike the previous paper of 2017, this study provides a comprehensive taxonomic account of each species (e.g., species name, collection site, synonyms, specimen vouchers, diagnoses, and figures). Additionally, all the species were assigned Korean names.
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채경희 ( Chae Kyung Hee ),최예환 ( Choi Ye Hwan ),연규석 ( Yeon Kyu Seok ),이윤수 ( Lee Youn Su ),주명기 ( Joo Myung Ki ) 한국농공학회 2003 韓國農工學會誌 : 전원과 자원 Vol.45 No.1
The effects of binder content and silica sand content on the durability characteristics of lightweight polymer concretes are examined. As a result, the flame lingering times using unsaturated polyester resin and non-combustible polyester resin were 60∼120 and 0∼4 seconds respectively, and the combustion lengths were 9∼11 mm and 0∼3 mm, respectively. Thus it is believed that the lightweight polymer concrete was incombustible and the light weight polymer concrete in which non-combustible material was added was perfectly non-combustible. The percent of original mass of lightweight polymer concrete, according to the freezing-thawing experiment, was below 0.3 %, which was much less than that of cement concrete. The pluse velocity, for the case of the binder content 28 %, showed the minimum decreasing rate for the lightweight polymer concrete with silica sand content of 50 %. The higher the binder content, the greater the durability. That is much higher than other material and believed that the freezing-thawing was suppressed by a low absorption.
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채경희,주명기,이윤수,연규석,최예환 한국농공학회 2003 한국농공학회논문집 Vol.45 No.1
The effects of binder content and silica sand content on the durability characteristics of lightweight polymer concretes are examined. As a result, the flame lingering times using unsaturated polyester resin and non-combustible polyester resin were 60∼120 and 0∼4 seconds respectively, and the combustion lengths were 9∼11 mm and 0∼3 mm, respectively. Thus it is believed that the lightweight polymer concrete was incombustible and the light weight polymer concrete in which non-combustible material was added was perfectly non-combustible. The percent of original mass of lightweight polymer concrete, according to the freezing-thawing experiment, was below 0.3 %, which was much less than that of cement concrete. The pluse velocity, for the case of the binder content 28 %, showed the minimum decreasing rate for the lightweight polymer concrete with silica sand content of 50 %. The higher the binder content, the greater the durability. That is much higher than other material and believed that the freezing-thawing was suppressed by a low absorption.
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교육 현장에서 시행된 임상 술기 시험의 다면적 타당도 분석
채한,이민정,김명호,김규석,조은별 대한한의학회 2024 대한한의학회지 Vol.45 No.1
Introduction: The importance of clinical skills training in traditional Korean medicine education is increasingly emphasized. Since the clinical skills tests are high-stakes tests that determine success in national licensing exams, it is essential to develop reliable multifaceted analysis methods for clinical skills tests in actual education settings. In this study, we applied the multifaceted validity evaluation methods to the evaluation results of the cardiopulmonary resuscitation module to confirm the applicability and effectiveness of the methods. Methods: In this study, we used internal consistency, factor analysis, generalizability theory G-study and D-study, ANOVA, Kendall's tau, descriptive statistics, and other statistical methods to analyze the multidimensional validity of a cardiopulmonary resuscitation test in clinical education settings over the past three years. Results: The factor analysis and internal consistency analysis showed that the evaluation rubric had an unstable structure and low concordance. The G-study showed that the error of the clinical skills assessment was large due to the evaluator and unexpected errors. The D-study showed that the variance error of the evaluator should be significantly reduced to validate the evaluation. The ANOVA and Kendall's tau confirmed that evaluator heterogeneity was a problem. Discussion and Conclusion: Clinical skills tests should be continuously evaluated and managed for validity in two steps of pre-production and actual implementation. This study has presented specific methods for analyzing the validity of clinical skills training and testing in actual education settings. This study would contribute to the foundation for competency-based evidence-based education in practical clinical training.
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채의수,김종광,손상균,Yoon Young Cho,Byung Min Ahn,문준호,Seoung Woo Jeon,박재용,In Taek Lee,최규석,Soo Han Jun 대한의학회 2008 Journal of Korean medical science Vol.23 No.3
Since vascular endothelial growth factor (VEGF) is known to be a potent pro-angiogenic factor, we evaluated the potential association of two VEGF gene polymorphisms (-634G>C and 936C>T) with the susceptibility and the clinicopathologic characteristics of colorectal cancer (CRC). The VEGF genotypes were determined using fresh colorectal tissue from 465 patients who had undergone a surgical resection and peripheral blood lymphocytes from 413 healthy controls by PCR/DHPLC assay. For the -634G>C polymorphism, the -634 GC or CC genotype was associated with a decreased risk of CRC (odds ratio [OR], 0.62; p=0.001) as a dominant model of C allele, whereas the 936 TT genotype correlated with advanced stage/ metastasis, a high serum level of CA19-9, and an higher grade in patients with CRC. In the haplotype analyses, haplotype -634C/936C and -634G/936T were associated with a decreased susceptibility of CRC (OR, 0.53 and 0.56; p<0.001, respectively). These observations imply that the VEGF gene polymorphisms may be associated with the susceptibility or clinicopathologic features of CRC. However, further studies of other VEGF sequence variants and their biological functions are needed to understand the role of the VEGF gene polymorphisms in the development and progression of CRC.
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조윤영,김종광,채의수,손상균,강병욱,문준호,전성우,박준석,박진영,최규석 대한암학회 2011 Cancer Research and Treatment Vol.43 No.3
Purpose Insulin-like growth factors (IGF) regulate a wide range of biological functions including cell proliferation, differentiation, and apoptosis through paracrine and autocrine mechanisms. Accordingly, the present study analyzed polymorphisms of IGF genes and their impact on the prognosis for patients with colorectal cancer. Materials and Methods Four hundred and two consecutive patients with curatively resected colorectal adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from fresh colorectal tissue and 8 polymorphisms of IGF genes determined using a real-time polymerase chain reaction genotyping assay. Results Pathologic stages after surgery were as follows: stage 0/I (n=85, 21.1%), stage II (n=147,36.6%), stage III (n=145, 36.1%), and stage IV (n=25, 6.2%). Multivariate survival analysis including stage, age, site of disease, and carcinoembryonic antigen level showed that the progressionfree survival for patients with the IGF2 +1280 GG genotype was slightly better than for the patients with the combined IGF2 +1280 AA and AG genotype (p=0.056), although there was no significant difference in the overall survival. However, the other polymorphisms were not associated with survival. Conclusion None of the 8 IGF1 or IGF2 gene polymorphisms investigated in this study were found to be independent prognostic markers for Korean patients with surgically resected colorectal cancer.
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문지혜,김강산,채규석,민기식,정재호 한국동물분류학회 2020 Animal Systematics, Evolution and Diversity Vol.36 No.2
From a moss sample, we isolated and identified Notohymena gangwonensis Kim et al., 2019 based on morphological and molecular data. The moss and type population has completely identical 18S rRNA (nuclear small subunit ribosomal RNA) gene sequences and both are highly similar in morphological and morphometric attributes, except for the diameter and arrangement of the cortical granules. Thus, we reexamined the type materials (i.e., micrographs and gDNA) and resulted in finding mistakes made by the authors of the species. Based on these data and supporting materials newly obtained (i.e., internal transcribed spacer [ITS] 1, ITS2, 5.8S, and partial 28S rDNA sequences, and scanning electron micrographs), we provide improved diagnosis of the species to clarify its identity. In addition, a key for Notohymena species is provided.
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