[통일의 길] 명사의 고향을 가다 : 차인태 아나운서, “통일이여, 어서 오라!”

차인태 평화문제연구소 2015 통일한국 Vol.381 No.-

지상파 3사 일일드라마에 나타난 호칭어.지칭어 관련 언어사용 사례 분석

차인태 경기대학교 연구교류처 2008 論文集 Vol.53 No.-

할리우드의 映畵産業 戰略과 韓國映畵産業의 新 進路

차인태,전평국 경기대학교 1999 論文集 Vol.43 No.1

This paper discusses the American movie industry, represented by Hollywood. and its foreign market strategy and its overall impact on the korean movie industry. Especially, this paper focuses on analyzing the current crisis of the Korea movie industry and developing the possible alternatives or new path for its reinvigoration by comparing the Production, distribution, and promotion system between the movie industries of the two countries. This paper argues that the current world dominance of the American movie industry can be attributed to its economies of scale, mass production, windowing effect through the horizontal and vertical integration among the relevant industries, strong marketing and distribution system, successful creation of derivative industries through the utilization of mass communication, and the export push and price differentiation policy of the American government. In contrast, this paper argues, the Korean movie industry is handicapped by insufficient movie infrastructure, emphasis on import of foreign movies rather than on national production of its own movies, meagerness of movie production capital, absence of the cooperation system or structure among the movie-relevant industries, and non-transparency of capital flowing for movie production. Following these arguments, this paper suggests that establishment of the strong infrastructure, creation of its own new image and brand for Korean movies, aggressive and efficient marketing strategy, and appropriate policy support of the government are necessary for the reinvigoration and development of the Korean movie industry.

Isolation, Expression, and Characterization of S-adenosyl-Lmethionine Synthetase from Lactobacillus paraplantarum 72815

차인태,남영도,서명지 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

The S-adenosyl-L-methionine (SAM) synthetase gene was mined from Lactobacillus paraplantarum 72815 isolated from the traditional Korean fermented food Kimchi. The gene was cloned and expressed in Escherichia coli to obtain recombinant SAM synthetase (SAM-s-LP). The purified SAM-s-LP was characterized as 40ºC and pH 8.5 as the optimal temperature and pH for activity, respectively. The apparent Km values of SAM-s-LP were 1.47±0.59 mM for L-Met and 2.84±0.82 mM for ATP.

PC통신 언어 분석

차인태 한국음성과학회 2001 음성과학 Vol.8 No.3

Nowadays, computer-mediated communications such as chatting and e-mail are widely used in younger generation. It has been known that the communication words used by on-line users (so-called netizens) are quite different from those used by ordinary people. That is, the words are generally characterized by shortened, mimetic and onomatopoeic words, and the netizens also use various types of emoticon expressed like signs or symbols. This can cause some orthographic as well as cultural problems. This paper investigates some statistical data in PC-based communications, analyzes the general characteristics, and probides some explanations.

Micrococcus sp. HJ19에서 체외분비 단백질 분해효소의 생산조건과 효소특성

차인태,오용식,조운동,임채성,이제관,이오석,노동현,Cha, In-Tae,Oh, Yong-Sik,Cho, Woon-Dong,Lim, Chae-Sung,Lee, Je-Kwan,Lee, Oh-Seuk,Roh, Dong-Hyun 한국미생물학회 2009 미생물학회지 Vol.45 No.1

단백질 분해효소는 아미노산 간에 존재하는 공유결합인 펩티드 결합을 절단하는 효소이며, 세계적으로 가장 많이 판매되는 효소이다. 해양 심층수로부터 분리된 Micrococcus sp. HJ19의 체외분비 단백질 분해효소를 생산하는 최적의 배지조건을 조사하기 위하여 기본배지로 변형 STY 배지(1% tryptone, 0.15% yeast extract, 0.01% NaCl, 여과살균한 바닷물)를 사용하였다. 탄소원으로 포도당보다 갈락토오스를 사용하였을 때 1.3배로 높은 활성을 보여주었으며, 질소원으로는 casamino acid를 사용하였을 때 가장 낮은 활성을 보여준 반면, 맥아추출물을 사용하였을 때 가장 높은 활성을 나타내었다. 생산된 체외단백질 분해효소는 $35^{\circ}C$에서 최적의 활성을 나타내었으며, 최적 pH는 8.5로 판명되었다. Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to investigate optimal medium compositions of carbon and nitrogen source for enzyme production, modified STY medium containing 0.15% yeast extract were used as basal medium. When galactose was used as carbon source, enzyme activity showed 1.3 higher than that of glucose. For nitrogen source addition of casamino acids to basal medium in place of tryptone showed lowest activity, whereas addition of malt extract showed maximal activity. The optimum temperature and pH of extracellular protease were found to be $35^{\circ}C$ an pH 8.5.

Tofua Arc의 열수구환경으로부터 호열성 혐기성 고세균(Thermococcus)의 농화배양 및 동정

차인태,김소정,김종걸,박수제,정만영,주세종,권개경,이성근,Cha, In-Tae,Kim, So-Jeong,Kim, Jong-Geol,Park, Soo-Je,Jung, Man-Young,Ju, Se-Jong,Kwon, Kae-Kyoung,Rhee, Sung-Keun 한국미생물학회 2012 미생물학회지 Vol.48 No.1

Hydrothermal vents (HTV) provide special environments for evolution of lives independent on solar energy. HTV samples were gained from Tofua arc trench in Tonga, South Pacific. We investigated archaeal diversity enriched using combinations of various electron donors (yeast extract and $H_2$) and electron acceptors [Iron (III), elemental sulfur ($S^0$) and nitrate. PCR amplification was performed to detect archaeal 16S rRNA genes after the cultures were incubated $65^{\circ}C$ and $80^{\circ}C$ for 2 weeks. The cultures showing archaeal growth were transferred using the dilution-to-extinction method. 16S rRNA gene PCR-Denaturing Gradient Gel Electrophoresis was used to identify the enriched archaea in the highest dilutions where archaeal growth was observed. Most of cultured archaea belonged to genus of Thermococcus (T. alcaliphilius, T. litoralis, T. celer, T. barossii, T. thoreducens, T. coalescens) with 98-99% 16S rRNA gene similarities. Interestingly, archaeal growth was observed in the cultures with Iron (III) and nitrate as an electron acceptor. It was supposed that archaea might use the elemental sulfur generated from oxidation of the reducing agent, sulfide. To cultivate diverse archaea excluding Thermococcus, it would be required to use other reducing agents instead of sulfide. 열수구(Hydrothermal vent)는 빛이 없는 환경에서 생명체의 진화가 일어나는 독특한 환경을 유지하고 있다. 남태평양 Tonga의 Tofua arc의 열수구로부터 퇴적물을 채취하여 산화철[iron(III)], 황(elemental sulfur, $S^0$) 그리고 질산염을 전자수용체로 사용하고, 수소($H_2$), yeast extract를 전자공여체로 사용하여 배양에 의한 미생물의 다양성을 연구하였다. 배양 온도는 각각 $65^{\circ}C$와 $80^{\circ}C$였으며, 연속희석배양법과 16S rRNA 유전자의 PCR-Denaturing Gradient Gel Electrophoresis를 분석하고, 검출된 염기서열의 정보분석을 통하여 고세균을 동정하였다. 16S rRNA 유전자의 계통분류학적 분석 결과 배양된 대부분의 고세균은 Thermococcus 속(T. alcaliphilius, T. litoralis, T. celer, T. barossii, T. thoreducens, T. coalescens)에 속하며 그들과 98-99%의 상동성을 가지고 있었다. Thermococcus 속의 미생물들이 일반적으로 이용할 수 없는 질산염과 산화철을 전자수용체로 첨가한 배양에서 관찰되었으나, 이는 환원제로 첨가한 $Na_2S$의 산화물을 이용하여 성장한 것으로 추정된다. Thermococcus 속에 속하는 고세균 외의 다양한 고세균의 배양을 위해서는 $Na_2S$ 대신 다른 환원제를 사용하는 배양조건의 이용이 요구된다.

단백질 분해효소를 분비하는 Micrococcus sp. HJ19의 분리 및 특성

차인태,오용식,노동현,Cha, In-Tae,Oh, Yong-Sik,Roh, Dong-Hyun 한국미생물학회 2007 미생물학회지 Vol.43 No.3

Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to screen new source of pretense, bacteria secreting extracellular pretense were isolated by enrichment culture from deep sea water samples of East Sea, Korea. A bacterium, named as HJ19, showed the best growth and the largest clear zone in plates supplemented skim milk at $30^{\circ}C$. The partial DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology identified that this strain was In genus Micrococcus. The strain HJ19 could not grow at $10^{\circ}C$ but it started growth and showed pretense activity at $20^{\circ}C$. The optimal growth was at $37^{\circ}C$ and the maximal protease activity at $30^{\circ}C$ was about 480unit/ml. 단백질 분해효소는 아미노산간에 존재하는 peptide결합을 절단하는 효소로 산업적으로 많이 사용된다. 이러한 단백질 분해효소의 새로운 공급원을 찾기 위하여 체외 단백질 분해효소를 생산하는 세균을 둥해 심층수로부터 집식배양으로 분리하였다. 이들 중 빠른 성장과 skim milk를 첨가한 배지에서 가장 큰 투명환을 보여준 균주를 HJ19로 명명하였다. 분리균의 형태학적, 생리생화학적 특성과 16S rRNA gene의 염기서열을 조사한 결과 Micrococcus sp.에 속하는 것으로 나타났다. 분리된 Micrococcus sp. HJ19는 $10^{\circ}C$에서는 자라지 못하였으며 $20^{\circ}C$부터 생육과 효소활성을 보여주었다. 최적 생육 온도는 $37^{\circ}C$였으며, 효소 활성은 $30^{\circ}C$에서 약 480 unit/ml의 활성을 보여 주었다.

동해 심층수로부터 Pseudoalteromonas sp. HJ 47의 분리 및 체외단백질분해효소 특성

차인태,임형준,노동현,Cha, In-Tae,Lim, Hayung-Joon,Roh, Dong-Hyun 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Proteases are enzymes that break peptide bonds between amino acids of other proteins and occupy a crucial position with respect to their applications in both physiological and commercial fields. In order to screen new source of protease, bacteria producing extracellular proteases at low temperature were isolated from deep sea water of East Sea, Korea. A bacterium showing the best growth rate and production of an extracellular protease at low temperature was designated HJ 47. The DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology led to the placement of this organism in the genus Pseudoalteromonas. Although maximal growth was observed at $37^{\circ}C$, enzyme production per culture time was maximum at $20^{\circ}C$. At this temperature, extracellluar protease production was detected from the end of the exponential phage to stationary phase, and maximal at 15 hours after initial production. The optimum temperature and pH of the protease were found to be $35^{\circ}C$ and 8. 단백질분해효소는 다른 단백질들의 아미노산 간에 존재하는 peptide 결합을 절단하며 생리학적, 상업적 측면에서 중요한 위치를 차지하는 효소의 한 부류이다. 이러한 단백질분해효소의 새로운 공급원을 찾기 위하여 비교적 저온에서 체외단백질분해효소를 생산하는 세균을 동해심층수로부터 분리하였다. 분리된 균 중 저온에서의 생육정도와 높은 활성을 가지는 균주를 선별하여 HJ 47이라 명명하였다. 형태학적, 생리생화학적 특성과 16s rRNA gene의 염기서열을 조사한 후 Pseudoalteromonas sp.에 포함하는 것으로 나타났다. 분리된 Pseudoalteromonas sp. HJ 47은 $10^{\circ}C$에서도 비교적 잘 자랐으며, $37^{\circ}C$에서 최적의 생육을 보여 주었다. 최적생육온도와는 달리 배양시간당 최대 체외단백질분해효소의 생산은 $20^{\circ}C$에서 최대였고 대수기 후반과 정지기에 생산이 시작되어 15시간 경과 후 최대의 생산을 보여주었다. 효소활성의 최적온도는 $35^{\circ}C$, 최적 pH는 8로 판명되었다.

위성방송의 산업조직론적 분석요소에 관한 연구

차인태 한국언론학회 2001 한국언론학회 학술대회 발표논문집 Vol.2001 No.-