과배란 방법이 Rat 수정란의 양과 질에 미치는 영향

진동일,양무희 韓國受精卵移植學會 1997 한국동물생명공학회지 Vol.12 No.2

본 연구는 rat에서 PMSG도는 FSH 처리에 의한 과배란 유도가 배란율과 수정란의 질에 미치는 영향을 알아보기 위해 호르몬 처리하고 교미시킨 후 4일령에 난관과 자궁을 세척하여 정상 8-세포기 난자와 비정상 난자를 조사하였고 각 처리에서 채란된 난자 중에 정상난자를 골라 체외 배양하여 발육율을 비교 평가하였다. 미성숙 rat에서는 평균19.1개의 수정란이 채취되었으며 성숙rat에서는 14.2개가 채취되었고 미성숙 rat에서는 성숙 rat에 비해 더

결핵균의 분리율과 오염율에 대한 선택배지 및 비선택배지 사용의 영향

진동일,우경자,정윤섭,이삼열 대한임상검사정도관리학회 1988 臨床檢査와 精度管理 Vol.10 No.1

성장관련 수용체 유전자를 이용한 형질전환토끼의 생산과 분석

진동일 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Transgenic rabbits were produced by DNA microinjection using growth hormone receptor (GHR) and IGF-I receptor (IGF-IR) genes. Overall efficiencies for production of transgenic rabbits were 3.2% and 3.1% in GHR and IGF-IR genes, respectively. Founder rabbits transmitted transgenes to their progenies through medelian fashion. The mRNA expression of transgenes using Northern blotting with probes of IGF-IR and GHR genes was checked in liver of transgenic rabbits. Transgenic rabbits with IGF-IR and GHR genes more expressed mRNA than control non-transgneic rabbits. Growth rate in GHR and IGF-IR transgenic rabbits was faster than non-transgenic rabbits. Transgenic rabbits grew larger (25% and 15% increase in body weight of GHR and IGF-IR transgenic rabbits, respectively) than non-transgenic rabbits and organ weight of transgenic rabbits increased. Double transgenic rabbits were produced through the mating between IGF-IR and GHR single transgenic rabbits. Transmission pattern of double transgenic mice were almost the same as that of single transgenic rabbits. Growth of double transgenic rabbits (IGF-IR/GHR) were fastest compared with transgenic rabbits containing IGF-IR and/or GHR genes. The experiments demonstrated that these growth-related genes could constitutively promote growth of female and male rabbits and transgenic rabbits with these genes could be used as model animals for growth-related studies of other livestock animals.

Survival of Single Blastomeres Isolated from 2-, 4- and 8-Cell Mouse Embryos after Freezing and Thawing

진동일 선문대학교 자연과학대학 1998 자연과학대학 논문집 Vol.1 No.-

본 연구는 2-, 4- 또는 8-세포기 생쥐 수정란으로부터 분리된 단일 할구의 발달 및 동결 후 생존능력을 조사하기 위하여 실시하였다. 2-, 4- 또는 8-세포기 생쥐 수정란에서 각각 223개, 60개, 188개의 할구를 분리하여 96시간 배양하였는데 이 중 2-세포기 수정란으로부터 분리된 할구는 111개(49.8%)가 배반포기까지 발육하였으며, 4-세포기와 8-세포기 수정란으로부터 분리된 할구는 각각 12개(20.0%)와 31개(16.5%)가 배반포기까지 발육하였다. 분리된 할구를 완만 동결한 후 융해하여 배양하여 배반포기까지의 발육율은 2-세포기 할구는 27.1%(16/50), 4-세포기 할구는 34.4% (4/11) 그리고 8-세포기 할구는 17.6%(3/17)였으며, 융해 후 할구 회수율은 각각 54.2%(65/120), 46.4% (13/28), 24.3%(17/70)을 나타내었다. 2-세포기 할구를 동결 융해한 후 동기화 시킨 생쥐 자궁에 이식하여 1마리가 정상적으로 발달하였고 융해하지 않은 할구로부터는 2마리의 태아가 발달하였다. The development of single blastomeres isolated from 2-, 4- and 8-cell mouse embryos and the ability of such blastomeres to survive deep freezing were studied. Of 223, 60 and 188 single blastomeres isolated from 2-, 4- and 8-cell mouse embryos, respectively, 111 blastomeres (49.8%) from 2-cell embryos, 12 blastomeres (20.0%)) from 4-cell embryos and blastomeres (16.5) from 8-cell embryos developed into blastocysts after culture for 96 hrs. The survival of frozen-thawed blastomeres from 2-. 4- and 8-cell embryos was 27.1% (16/50), 34.4% (4/11) and 17.6% (3/17), and the recovery rate was 54.2% (65/120), 46.4% (13/28) and 24.3% (17/70), respectively. One apparently normal fetus was obtained from the transferred frozen-thawed blastomere from 2-cell embryos and two fetuses from the transferred unfrozen blastomeres.

The Biological Role of ras Signaling Pathway in Monocytes

진동일 암연구센터 1996 암연구의최신지견 Vol.1996 No.-

토끼의 배아세포 분리에 관한 연구

진동일 선문대학교 ·중소기업기술지원연구소 1999 선문공대 연구/기술 논문집 Vol.4 No.1

토끼 배아세포(Embryonic Stem Cell)를 분리하기 위해 토끼 1-cell embryo를 채란하여 in vitro에서 blastocyst까지 배양한 후 mouse embryonic fibroblasts(MEF), rabbit embryonic fibroblasts(REF) 및 STO cell expressing Leukemia Inhibition Factor gene(SNL) feeder cell과 공배양하였다. 외관상 충실한 토끼 배아세포 8 개를 확보하였고 분리된 토끼 ES ceIl의 모양은 주위에 분화된 세포가 없는 전형적인 colony모양으로 성장하고 액체질소에 동결보존 및 3-5차례의 계대배양 후에도 이러한 모양은 계속 유지되었다 충분히 자란 dish를 1 : 2로 계대배양을 한 후 다시 confluent하게 자라는 데에 걸리는 시간(doubling time)은 빠른 경우 84시간으로 나타났다. 분리된 토끼 ES cell은 gelatin이 coating되지 않은 culture dish에 이식 배양하였을 때 부유상태로 증식하면서 내부에 강(腔)이 생기고 외배엽과 내배엽이 형성하는 전형적인 Embryoid body 모양을 나타내어 분리된 ES cell이 미분화상태의 stem cell임이 확인되었다. To establish rabbit Embryonic Stem (ES) cells, rabbit one-cell embryos were collected and cultured in vitro to blastocysts. Blastocysts were co-cultured with mouse embryonic fibroblasts (MEF), rabbit embryonic fibroblasts (REF) or STO cells expressing LIF (SNL). Although rabbit ES cells were isolated with low efficiencies, total 8 ES cell lines were kept in vitro with normal colony shape. Their doubling time was about 84 hours and their shapes were maintained with passing and freezing. These rabbit ES cells were differentiated into embryoid body following the culture in the uncoated dishes which indicated that they were undifferentiated stem cells.

Tetraploidy Induction of Mouse Embryos by In Vitro Culture with Cytochalasin B

진동일 한국수정란이식학회 1999 한국동물생명공학회지 Vol.14 No.2

효율적인 homozygous동물을 생산하기 위한 실험의 단계로 염색체가 4배체인 수정란의 이용성을 타진하기 위해 생쥐 수정란과 cytochalasin B를 사용하여 4배체 유도에 관한 실험을 수행하였다. 생쥐 2-세포기 수정란을 10 ㎍/ml 농도의 cytochalasin B로 약 20시간 배양하였을 때 모든 수정란은 발육을 거의 멈추었으나, 이 수정란을 cytochalasin B-free medium에 체외배양 하였을 때 발육이 재개되어 48시간 후 상실기나 배반포기까지 약 74%의 발육율을 나타내었다. 그러나 발육된 수정란의 세포수는 대조구에 비해 훨씬 적은 것으로 나타났다. 염색체 분석결과 cytochalasin B로 처리한 대부분의 수정란은 4배체인 것으로 나타났고 약간의 수정란은 mosaicism과 다배체를 나타내기도 하였다. 그러므로 cytochalasin B를 이용하여 효과적으로 4배체의 수정란을 유도할 수 있는 것으로 나타났다.

Effects of Slow Freezing on Development of Blastomeres Separated from Mouse Preimplantation Embryos

진동일 한국가축번식학회 2000 Reproductive & developmental biology Vol.24 No.3

The development of single blastomeres isolated from 2-, 4- and 8-cell mouse embryos and the ability of such blastomeres to survive slow freezing were studied. Of 223, 60 and 188 single blastomeres isolated from 2-, 4- and 8-cell mouse embryos, respectively, 111 blastomeres (49.8%) from 2-cell embryos, 12 blastomeres (20.0%) from 4-cell embryos and blastomeres (16.5) from 8-cell embryos developed into blastocysts after culture for 96 hrs. The recovery rate was 54.2% (65/120), 46.4% (13/28) and 24.3% (17/70) of blastomeres derived from 2-, 4- and 8-cell embryos following freezing and thawing and the survival of frozen-thawed blastomeres was 27.1% (16/59), 36.4% (4/11) and 17.6% (3/17), and respectively. The apparently six normal fetuses were obtained from frozen-thawed blastomere from 2-cell embryos after transferring into the recipients. These results indicate that mouse blastomeres isolated from preimplatation stage embryos can survive storage in liquid nitrogen following slow freezing.

동해방지제 , 식빙 , 동결속도 및 보존기간이 생쥐 초기배의 생존성에 미치는 영향

진동일,임경순,오봉국,이용빈 ( D . I . Jin,K . S . Im,B . K . Ohh,Y . B . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.7

To study the factors affecting cryopreservation of mouse embryos, 6-12 week old ICR mice were used. Embryos were dehydrated in IPBS medium containing DMSO or Glycerol by 3 step procedure and cooled to -50℃ at different cooling rates (1.0, 3.0, 5.0, 7.0 and 10.0℃/min) and plunged into liquid nitrogen (-196℃) and stored for 12hrs to 30 days. Embryos were thawed in air to room temperature at rate of about 20℃/min and rehydrated by 3 step procedure. When the embryos were dehydrated in the medium containing cryoprotectants, then survival rates were decreased compared to embryos which did not dehydrated, and same trend was noted when the ice nucaeation of embryos was induced at -5℃. The survival rate of embryos dehydrated or cooled to -5℃ showed no difference between 8-cell and morula stage embryos. The survival rate of embryos was best (45%) when the cooling rate was 1℃/min, but it was decreased when the cooling rate was increased. Embryos could be stored up for 15-30 days without significant decrease in their survival rate when they were cooled at 1℃/min to -50℃ and stored in -196℃.

복제 소와 생쥐에서 태반 발육이상에 관한 분자생물학적 연구

진동일,김홍래,Naruse, Kenji,이혜란,윤종택,Wakayama, Teruhiko,박창식 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10