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진기옥,양성기 제주대학교 해양과환경연구소 2001 해양과환경연구소 연구논문집 Vol.25 No.-
The number of developed groundwater wells on Jeju island is approximately 5,100 and 245 of them are located in the mountainous area whose altitude varies from 200m to 600m. Since the development and usage of groundwater on hillsides started, a detailed investigation on groundwater characteristics, such as the distribution of the geologic stratum, groundwater occurring characteristics, content and amount of gainable groundwater, has been investigated. In this study, groundwater occurring characteristics are examined through the evaluation of drawdown and level fluctuation based on the distributive type found in the geologic stratum in the mountains. The higher the land elevation is, the deeper the absolute depth of natural and balanced groundwater level occurs. However drawdown, which show 25.7m, 24.63m, 13.8m and 12.5m, respectively at each elevation, tends to decrease as ground elevation increases. It is found that more than half of the transmissible geologic structure of Sumgol, Gotjawal, Oreum, or small river is distributed over the hillside area from data examined by Jeju province. Although it helps groundwater recharge, it does not have direct relationship with the transmissivity which is represented by the capacity of the groundwater supply. The capacity of groundwater supply mainly depends on the distributive type of the aquifers that poses low permeablilty.
진기옥,양성기 제주대학교 해양과환경연구소 2001 해양과환경연구소 연구논문집 Vol.25 No.-
As the quality of life and tourism have improved, various facilities have been established on hillsides which have excellent views and consequentiy development of groundwater has been promoted. At present the number of developed groundwater wells the in Jeju island is around 5,100 and 245 of them are located in the mountainous area whose altitude varies from 200m to 600m. Since the development and usage of groundwater on hillsides have stared activated, the detailed investigation of groundwater characteristics, such as the distribution of geologic stratum, understanding of hydraulic properties, the content and the amount of gainable groundwater, has been investigated required. In this study, the hydraulic characteristics are examined through the evaluation of drawdown and transmissibility based on the distributive type of geologic stratum in the mountains. Classes of 201∼300m, 301∼400m, 401∼500m and 501∼600m show transmissivities of 183.5㎡/d, 41.11㎡/d, 631.02㎡/d and 1,162.65㎡/d respectively, which means that transmissivity increases as elevation grows. Their average value is 292.19㎡/d, higher than 201.8㎡/d that is estimated from data of 279 wells which have developed in lowlands until 1998.
α-1,3-Galactosyltransferase Knock Out(GalT KO) 돼지유래 골수 중간엽 줄기세포의 특성 규명
옥선아,오건봉,황성수,임석기,김영임,박진기 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.3
A major barrier to progress in pig to primate organ transplantation or cell therapy is the presence of terminal α -1,3-galactosyl epitopes on the surface of pig cells. Therefore, the purpose of this experiment was to establish and cha- racterize mesenchymal stromal/stem cells (MSCs) derived from α-1,3-galactosyltransferase (GalT) knock out (GalT KO) pig to confirm their potential for cell therapy. Bone marrow (BM)-MSCs from GalT KO pig of 1 month old were isolated by Ficoll-Paque PLUS gradient and cultured with A-DMEM + 10% FBS on plastic dishes in 5% CO2 incubator at 38.5. GalT KO BM-MSCs were analyzed for the expression of CD markers (CD45-, 29+, 90+ and 105+) and in vitro differentiation ability (adiopogenesis and osteogenesis). Further, cell proliferation capacity and cell aging of GalT KO BM-MSCs were compared to Wild BM-MSCs by BrdU incorporation assay (Roche, Germany) using ELISA at intervals of two days for 7 days. Finally, the cell size was also evaluated in GalT KO and Wild BM-MSCs. Statistical analysis was performed by T-test (P<0.05). GalT KO BM-MSCs showed fibroblast-like cell morphology on plastic culture dish at passage 1 and exhibited CD45-, 29+, 90+ and 105+ expression profile. Follow in ginduction in StemPro adipogenesis and osteogenesis media for 3 weeks, GalT KO BM-MSCs were differentiated into adipocytes, as demonstrated by Oilred Ostaining of lipid vacuoles and osteocytes, as confirmed by Alizarinred Sstaining of mineral dispositions, respectively. BrdU incorporation assay showed a significant decrease in cell proliferation capacity of GalT KO BM-MSCs compared to Wild BM-MSCs from 3 day, when they were seeded at 1x103 cells/well in 96-well plate. Passage 3 GalT KO and Wild BM-MSCs at 80% confluence in culture dish were allowed to form single cells to calculate cell size. The results showed that GalT KO BM-MSCs (15.0 ± 0.4 μm) had a little larger cell size than Wild BM-MSCs (13.5 ± 0.3 μm). From the above findings, it is summarized that GalT KO BM-MSCs possessed similar biological properties with Wild BM-MSCs, but exhibited a weak cell proliferation ability and resistance to cell aging. Therefore, GalT KO BM-MSCs might form a good source for cell therapy after due consideration to low proliferation potency in vitro.
열-구조 연성해석에 의한 알루미늄 6061 마찰교반용접 특성 변수 연구
박찬우,팽진기,옥주선 한국항공우주학회 2011 한국항공우주학회 학술발표회 논문집 Vol.2011 No.11
마찰교반용접(FSW)과정에서의 모재의 변형과 온도분포를 연구하기 위해 A fully coupled thermomechanical model 을 적용하였다. 본 연구의 주요변수는 용접 툴의 회전속도이며, 다른 주요변수에 따른 영향을 3 차원해석 결과로 나타내었다. 본 해석 결과, 최대온도가 용접모재의 용융점이하에서 나타남을 알 수 있다. 본 수치 모델에서는 용접모재의 leading side 에서 높은 온도구배가 발생하며, 회전 각속도가 증가할수록 최대 온도가 증가함을 알 수 있다. 본 연구는 ABAQUS Ver.6.7 로 시뮬레이션을 수행하였다. 또한 마찰교반용(FSW)과정에서의 대 변형은 ALE(Arbitrary Lagrangian-Eulerian) finite element formulation 을 사용하였으며, Mass scaling 을 사용하여 해석 시간을 줄일 수 있었다. A fully coupled thermo-mechanical model is adopted to study the temperature distribution and the material deformation in friction stir welding (FSW) process. Rotational speed is most important parameter in this research. Three dimensional analysis results under different process parameters were presented. Results indicate that the maximum temperature is lower than the melting point of the welding material. The higher temperature gradient occurs in the leading side of the workpiece. The maximum temperature can be increased with increasing the tool angular velocity, rpm in the current numerical modeling. In this research ABAQUS Ver.6.7 is used to analyze a fully coupled thermo-mechanical model. ALE(Arbitrary Lagrangian-Eulerian) finite element formulation is used for the large deformation in FSW process and the Mass scaling is used for analysis time efficiency.
권대진,오건봉,옥선아,이정웅,이승수,박진기,장원경,황성수 사단법인 한국동물생명공학회 2013 Reproductive & developmental biology Vol.36 No.4
This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.