Inducible nitric oxide synthase(iNOS) is synthesized by human gingival tissue and cultured human gingival fibroblasts

지숙,김흥중,국중기,김병옥 대한치주과학회 2003 대한치주과학회 학술대회자료집 Vol.2003 No.2

전치부 심미와 치주 건강을 위한 치관 연장술 : 문헌 고찰 및 증례보고 Rationale and Report of Cases

지숙,손경준,김상목 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

Clinical crown lengthening is a useful procedure to provide tooth length for proper restorative of a tooth without compromising the periodontium or the retentive qualities of the restoration. It is also useful for esthetic enhancement in patients with gingival hyperplasia and a "gummy smile" due to incomplete exposure of the anatomic crown. Total treatment could involve endodontic. orthodontic. periodontic . and restorative procedures for anterior esthetics. Careful evaluation. case selection, treatment planning, and surgical treatment following the principles outlines in this article can achieve results that meet the functional and esthetic challenges of current dental practice .

한 은둔형 외톨이 청년이 ‘자립을 이루어가는 경험’에 대한 내러티브 탐구

지숙,구자경 한국독서치료학회 2023 독서치료연구 Vol.15 No.3

This study is a narrative exploration of a reclusive loner youth’s experience and meaning of voluntary self-reliance in seclusion. The research participant, Inho (a pseudonym), is a 29-year-old reclusive young man, and the data were generated primarily through five interviews of two hours each between September 6, 2022, and May 31, 2023, as well as occasional online conversations. Data analysis was conducted using the narrative inquiry procedure proposed by Clandinin and Connelly (2000), and when writing the research text, the story was told in a three-dimensional narrative inquiry space to understand Inho’s experience and construct its meaning. Consequently, Inho’s narrative was reconstructed with the following meanings: 1) leaving the life of a victim of violence to become the master of one’s own life; 2) discovering one’s talents and dreams; 3) facing one’s own deficiencies; and 4) building a sense of solidarity. Inho’s experience of achieving self-reliance was based on his individual will to be self-reliant and was achieved through the cooperation of social, economic, and emotional self-reliance. We hope that Inho’s story will help psychological counselors and reading therapists who work with reclusive loners and their families to understand them and support their independence.

Effect of aging on expression of nitric oxide and inducible nitric oxide synthase in human gingival fibroblasts

지숙,국중기,박주철,김흥중,장현선,김종관,김병옥,Ji, Suk,Kook, Jung-Ki,Park, Joo-Cheol,Kim, Heung-Joong,Jang, Hyun-Seon,Kim, Chong-Kwan,Kim, Byung-Ock The Korean Academy of Periodontoloy 2006 Journal of Periodontal & Implant Science Vol.36 No.2

치주질환의 진행이 나이에 의해 영향을 받는다는 사실은 알려져 있으나 노화에 따른 치주조직 세포의 기능적인 변화에 관한 사실은 많이 알려져 있지 않다. 노화에 따른 세포의 노화가 치주질환의 진행에 어떠한 여향을 끼치는가를 아는 것은 중요하다. 염증 상태에서 nitric oxide (NO)는 조직 파괴에 관여하는 인자로 작용하여 치주질환의 진행에 관여하는 것으로 알려져 있다. 따라서 이 연구는 사람의 치은에서 배양된 치은섬유아세포를 이용하여 세포의 노화에 따른 NO와 이의 합성효소인 inducible nitric oxide synthase (iNOS)의 발현을 알아봄으로써 세포의 노화가 치주질환의 진행에 끼치는 영향에 대해 알아보고자 하였다. 10세의 환자와 55세의 환자에서 각각 채취한 치은에서 배양된 세포와 10세의 환자에서 채취한 세포를 계속적인 계대배양을 통해 얻은 실험실 상 노화된 세포를 포함하여 총 3 종류의 치은섬유세포를 실험에 이용하였다. Hot phenol-water extraction을 통해 추출된 Porphyromonas, gingivalis ATCC 33277 lipopolysaccharide (LPS)와 재조합 $IFN-{\gamma}$ 를 세포에 적용시켜 Griess assay를 통해 조건화된 배지에서 NO를 측정하였다. 20세와 55세의 환자에서 채취된 치은 조직과 총 3 종류의 배양된 세포에 NOS-II 항체를 적용시켜 iNOS 단백질 발현을 관찰하였다. Total RNA를 추출하여 RT-PCR를 통해 iNOS mRNA의 발현을 분석하였다. 치은섬유아세포에서 NO는 자발적으로 발생되었고, 이러한 발현은 젊은 세포보다 노화된 세포에서 강하였다. P, gingivalis LPS와 제조합 $IFN-{\gamma}$는 치은섬유아세포에서 NO의 발현을 증가시켰고, 이러한 발현은 젊은 세포보다 노화된 세포에서 강하였다. 면역조직화학 염색에서 iNOS 단백질은 젊은 사람과 노화된 사람의 치은 조직 모두에서 치은섬유아세포와 상피의 기저층 세포와 염증세포에서 발현되었으나 노화에 따른 발현의 차이를 구별할 수는 없었다. 세포의 면역염색에서 iNOS 단백질은 노화된 세포에서 강하게 발현되었고 이러한 발현은 LPS와 $IFN-{\gamma}$ 에 의해 강화되었다. LPS와 $INF-{\gamma}$ 의 조건이 주어지지 않은 상태에서 iNOS mRNA는 젊은 세포에서보다 노화된 세포에서 강하게 발현되었다. 이러한 결과를 통해 세포의 노화가 NO와 iNOS 발현을 증가시킴으로서 치주질환의 진행에 영향을 끼칠 수 있음을 시사하였다.

수직 치근 파절의 임상적 및 방사선적 연구

지숙,정승우,황규영 조선대학교 구강생물학연구소 2003 口腔生物學硏究 Vol.27 No.1

Vertical root fractures have been described as longitudinally oriented fractures of the root, extending from the root canal to the periodontium. They usually occur in endodontically treated teeth, although occurrence in non-restored teeth has been described. It can traverse the root in different directions mesially/distally or facially/lingually and may or may not involve the pulpal chamber. If unrecognized, vertical root fractures can lead to frustration and inappropriate endodontic treatment. Diagnosis is sometimes difficult as there is often no single clinical feature which indicates that root fracture is present and sings and symptoms are often delayed. Vertical root fractures that involved the gingival sulcus/pocket area usually have a hopeless prognosis due to continuous bacterial invasion of the fracture space from the oral environment. Single-rooted teeth will have to be extracted. In multi-rooted teeth a treatment alternative is hemisection and extraction of the fractured root. Where successful outcomes of conservative treatment have been claimed, the long-term prognosis has yet to be proven. This study reviews the clinical characteristics, diagnostic signs, and possible causes of vertical root fractures and reports 3 cases of vertical root fractrues.

Innate immune response to oral bacteria and the immune evasive characteristics of periodontal pathogens

지숙,최영님 대한치주과학회 2013 Journal of Periodontal & Implant Science Vol.43 No.1

Periodontitis is a chronic inflammation of periodontal tissue caused by subgingival plaque-associated bacteria. Periodontitis has long been understood to be the result of an excessive host response to plaque bacteria. In addition, periodontal pathogens have been regarded as the causative agents that induce a hyperinflammatory response from the host. In this brief review,host-microbe interaction of nonperiodontopathic versus periodontopathic bacteria with innate immune components encountered in the gingival sulcus will be described. In particular, we will describe the susceptibility of these microbes to antimicrobial peptides (AMPs) and phagocytosis by neutrophils, the induction of tissue-destructive mediators from neutrophils,the induction of AMPs and interleukin (IL)-8 from gingival epithelial cells, and the pattern recognition receptors that mediate the regulation of AMPs and IL-8 in gingival epithelial cells. This review indicates that true periodontal pathogens are poor activators/suppressors of a host immune response, and they evade host defense mechanisms.

재생된 골과 자연골 사이의 계면에 대한 유한요소법적 분석

지숙,이상화,김수관,김병옥 대한악안면성형재건외과학회 2004 Maxillofacial Plastic Reconstructive Surgery Vol.26 No.1

Intracellular Degradation of Fusobacterium nucleatum in Human Gingival Epithelial Cells

지숙,Ji Eun Shin,Yong Cheol Kim,Youngnim Choi 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.6

The role of Fusobacterium nucleatum in oral health and disease is controversial. We have previously shown that F. nucleatum invades gingival epithelial cells. However, the destiny of the internalized F. nucleatum is not clear. In the present study, the intracellular destiny of F. nucleatum and its cytopathic effect on gingival epithelial cells were studied. The ability of F. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (HOK-16B) cells were compared by confocal microscopy and flow cytometry. F. nucleatum had the highest invasive capacity, comparable to that of Porphyromonas gingivalis, a periodontal pathogen. Confocal microscopic examination revealed colocalization of internalized F. nucleatum with endosomes and lysosomes. Examination by transmission electron microscopy revealed that most intracellular F. nucleatum was located within vesicular structures with single enclosed membranes. Furthermore, F. nucleatum could not survive within gingival epithelial cells and had no cytopathic effects on host cells. Interestingly,endosomal maturation played a role in induction of the antimicrobial peptides human beta defensin (HBD)-2 and -3 by F. nucleatum from gingival epithelial cells. F. nucleatum is destined to enter an endocytic degradation pathway after invasion and has no cytopathic effect on gingival epithelial cells, which may cast new light on the role of F. nucleatum in the pathogenesis of periodontitis.

日本における法学研究者養成をめぐる現状と課題

指宿信(Makoto Ibusuki) 전북대학교 법학연구소 2019 法學硏究 Vol.61 No.-

Multivariate Analysis of the Predictors of Survival for Patients with Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization: Focusing on Superselective Chemoembolization

지숙경,조윤구,안용식,김미영,박윤옥,김재균,김완태 대한영상의학회 2008 Korean Journal of Radiology Vol.9 No.6

Objective: While the prognostic factors of survival for patients with hepatocellular carcinoma (HCC) who underwent transarterial chemoembolization (TACE) are well known, the clinical significance of performing selective TACE for HCC patients has not been clearly documented. We tried to analyze the potential factors of disease-free survival for these patients, including the performance of selective TACE. Materials and Methods: A total of 151 patients with HCC who underwent TACE were retrospectively analyzed for their disease-free survival (a median follow-up of 23 months, range: 1-88 months). Univariate and multivariate analyses were performed for 20 potential factors by using the Cox proportional hazard model, including 19 baseline factors and one procedure-related factor (conventional versus selective TACE). The parameters that proved to be significant on the univariate analysis were subsequently tested with the multivariate model. Results: Conventional or selective TACE was performed for 40 and 111 patients, respectively. Univariate and multivariate analyses revealed that tumor multiplicity, venous tumor thrombosis and selective TACE were the only three independent significant prognostic factors of disease-free survival (p = 0.002, 0.015 and 0.019, respectively). Conclusion: In our study, selective TACE was a favorable prognostic factor for the disease-free survival of patients with HCC who underwent TACE. Objective: While the prognostic factors of survival for patients with hepatocellular carcinoma (HCC) who underwent transarterial chemoembolization (TACE) are well known, the clinical significance of performing selective TACE for HCC patients has not been clearly documented. We tried to analyze the potential factors of disease-free survival for these patients, including the performance of selective TACE. Materials and Methods: A total of 151 patients with HCC who underwent TACE were retrospectively analyzed for their disease-free survival (a median follow-up of 23 months, range: 1-88 months). Univariate and multivariate analyses were performed for 20 potential factors by using the Cox proportional hazard model, including 19 baseline factors and one procedure-related factor (conventional versus selective TACE). The parameters that proved to be significant on the univariate analysis were subsequently tested with the multivariate model. Results: Conventional or selective TACE was performed for 40 and 111 patients, respectively. Univariate and multivariate analyses revealed that tumor multiplicity, venous tumor thrombosis and selective TACE were the only three independent significant prognostic factors of disease-free survival (p = 0.002, 0.015 and 0.019, respectively). Conclusion: In our study, selective TACE was a favorable prognostic factor for the disease-free survival of patients with HCC who underwent TACE.