황색종 잎담배의 엽면지질 성분 조성 및 건조중 변화

지상운,김도연,이문용,김영희,신승구 한국연초학회 1997 한국연초학회지 Vol.19 No.1

The composition of leaf surface lipid in Flue-cured tobacco and their changes during curing was investigated. The flue-cured variety, Nicotiana tabacum cv. NC 82 was cultivated at Eumsung experiment station in 1996. The samples of riced leaves with different stalk Position(Lugs, Cutter, Leaf and Tips) and different curing stage at half yellowing(24hr), yellowing(48 hr) , color axing(72 hr), midrib drying(96 hr) and cured(120 hr) were collected for analysis of leaf surface lipid. $\alpha$ - and $\beta$ - 4, 8, 13-Duvatriene-1, 3-diol($\alpha$, $\beta$-DVT) were major components in leaf surface lipid extracted with methylene chloride and sugar ester was detected slightly DVT content was increased with ascending stalk position, but increasing trend of total hydrocarbon was not observed. While DVT was decreased throughout curing of tobacco leaves, hydrocarbon content did not show significant change during curing process. Twenty-two duvane compounds were detected by capillary GC in duvane fraction isolated from leaf surface lipid and of which 11 compounds were identified by GC-MS. These compounds were decreased with curing in all stalk position.

잎담배중 Duvatrienediol에 관한 연구

지상운,안기영,이문수,박영수,정찬선 한국연초학회 1992 한국연초학회지 Vol.14 No.2

It would be clear that the constituents of the leaf surface lipid is ye비 important as an evaluation index of tobacco leaf quality since the quality of tobacco specific aroma with leaf species depends on the contents of the lipid and the strength of the aroma is determined by the amounts of the lipid secreted. For the reason, a rapid and peproducible method to quantify DVT, which is a kind of lipid, has been studied. The biosynthesis procedure of DVT in leaf growing processes, and the volatile or decompositional characters of DVT in leaf drying processes were also discussed. In consequence, it might be possible to get the data available to the cultivation of better tobacco leaf and the manufacture of cigarettes with better aroma and taste. The results obtained from this study are as follows. 1. Chloroform/dichloromethane solvent was better than chloroform alone for DVT extraction. The extraction yields of the leaf surface lipid were about 5% 2. The extractives with dichloromethane were treated by silylation with BSTPa and the quantitative analysis of DVT was carried out using SE -54 fused silica capillary column. It was found that rapid and reproducible data could be obtained from these methods. 3. In flue - cured tobacco species, DVT contents were $30.3\mu\textrm{g}/cm^2$ in the beginning stage of leaf drying processes and $12.1\mu\textrm{g}/cm^2$ corresponded to 30% levels of the beginning stage, in the end stage. 4. DVT contents in Burley mere 2 times as large as those in fluecured tobacco. DVT in the upper stalk position of leaf was 3 times larger than that in the lower stalk position. 5. DVT of tobacco leaves was decomposed by $SO_2$ gas or the sun light. The decomposition rate was largest in the sample used methanol as a extraction solvent.

제품담배의 공기희석율에 따른 주류연중 휘발성 유기화합물의 이행특성

지상운,황건중,이문수,신창호,김수호,김종열,김미주,Ji Sang-Un,Hwang Keon-Joong,Rhee Moon-Soo,Shin Chang-Ho,Kim Soo-Ho,Kim Jong-Yeol,Kim Mi-Ju 한국연초학회 2004 한국연초학회지 Vol.26 No.2

This study was conducted to investigate the delivery pattern of volatile organic compounds(VOCs) in mainstream smoke generated by the combustion of a different ventilated cigarette. To compare the delivery pattern and the concentration of VOCs in mainstream smoke, the six different ventilated cigarette was manufactured and analyzed VOCs using the GC/MS. As a result of this experiments, cambridge filter used to trap the particulate matter in mainstream smoke did not affect on the trapping of VOCs components, and two impinger method among the trapping methods was the best condition to trap VOCs from mainstream smoke. As the slope of the delivery of VOCs such as isoprene, acrylonitrile and toluene were higher than 1, but that of benzene was lower than other VOCs.

Gas chromatography-Thermal Energy Analyzer에 의한 휘발성 니트로소아민과 담배 특유의 니트로소아민들의 동시 분석연구

이문수,지상운,박영수,Rhee, Mun-Su,Ji, Sang-Woon,Park, Yang-Su 한국연초학회 1993 한국연초학회지 Vol.15 No.2

This is to investigate the methodology for the simultaneous determination of Wk, mk and TSNA using gas chromatography(GC) in combination with chemiluminescence detector, thermal energy analyzer(TEA) . The simultaneous analysis has been estimated by evaluating tobacco. The TEA was linked to GC equipped with non -polar SPB -5 fused silica capillary column which was introduced into the ceramic pyrolysis tube by the point of 16cm from the end of TEA. Quantification was carried out by internal standardization with WDPA after calibration of retention times and response factors with authentic nitrosoamines. It was demonstrated that WDPA was most preferable as internal standard for the simultaneous analysis. The recoveries of the internal standard were in the range of 83∼96% . Nitrosoamines in this method were detected with determination limit of 0.1ng and was made by a straight line in calibration curve by TEA response. The suitability of nitrosoamines extraction in tobacco leaf was investigated. It was most suitable to extract nitrosoamines from tobacco leaves with 0.01 M NaOH within a period of 8 hours. Thimerosal as an antibacterial agent was added to NaOH solution to prevent artifactual formation. The fractionation and the purification of nitrosoamines form alkaline extracts were conveniently performed using Extrelut multilayer column and dichloromethane. Reproducible and reliable results were obtained for the determination of nitrosamines in a relatively short time compared to previous known method. TSNA contents in burley were about 4 times higher as those in the fluecured tobacco.

궐련담배 연기 응축물의 pH에 관한 연구

안기영,지상운 한국연초학회 1990 한국연초학회지 Vol.12 No.1

Cigarette smoke is an aerosol consisted of both vapor and Particulate phases. In this study, the smoke condensates from several blended cigarettes were dissolved in water and the pH of the resulting solution was determined. The pH of the smoke condensate decreased with increase of puff number. In the beginning the pH decreased a little, while decreased remarkably from puff No.5 to puff No.10.

ORS-ICP-MS를 이용한 담배 연기의 중금속 성분 분석

조성일,지상운,황건중,Cho, Sung-Eel,Ji, Sang-Un,Hwang, Keon-Joong 한국연초학회 2008 한국연초학회지 Vol.30 No.1

In this study, we compared three different pretreatment methods, such as block digestion, microwave digestion and solvent injection, coupled with Octopole Reaction System(ORS)-ICP-MS was used for the quantification of heavy metals in mainstream smoke of 2R4F reference cigarette, and then evaluated those efficiencies in the recovery, repeatability and reproducibility. In all methods, volatile heavy metals, As, showed the highest CV value, and Cd showed the lowest one. However, the solvent injection method showed the most stable recovery rate and CV value of 2.36 %. This method showed also advantages in time-consuming and compatibility with ICP-MS system. This results demonstrate that solvent injection method can be recommended as a superior pretreatment procedure to be able to reduce contaminants and spectral interference as well as loss of the elements interested.

Comparative Smoke Analysis of CORESTA Monitoring Cigarettes

이승용,지상운,신해근,Lee, Seung-Yong,Ji, Sang-Un,Shin, Hea-Geun The Korean Society of Tobacco Science 2011 한국연초학회지 Vol.33 No.1

This experiment was conducted as a part of Asia collaborative study on purpose of verifying the difference between CM6 and CM7 including 3R4F for reference. It carried out using various analytical categories for example, main stream, sidestream and Av. smoke. Additional analysis such as physical properties, blending ratio, combustibility and general leaf component analysis also implemented in order to investigate the difference. We complied with ISO standard and CORESTA recommended method during analytical operating procedures. In this study, we described that comparative analytical result for CM6 and CM7 known as reference or monitoring cigarettes including 3R4F for reference. All sample cigarettes were conditioned at $22^{\circ}C$, 60% relative humidity for 48 hours. Av. Smoke, MS and SS smoke analysis were performed over five times with two smoking condition, ISO and Health Canada with the exception of Av. smoke analysis. We complied with ISO standard method during analytical operating procedures. And, we conducted additional analysis, such as physical properties, blending ratio, combustibility and leaf component analysis also in order to investigate the difference. In conclusion, we found out some differences between CORESTA monitoring cigarette No. 6 and No 7. The smoke components such as total particulate matters, NFDPM, nicotine and carbon monoxide contents of CM7 were a little lower than CM6. And, these phenomena were the same as not only main stream smoke but also side stream smoke and Av. smoke. This tendency was consistent with ISO and Health Canada smoking condition. Besides, leaf constituents' color of CM7 was darker than CM6. In case of combustibility, it showed short combustion time approximately 30 seconds.

두 점 교정법을 이용한 담배 연기 성분 중 니코틴 분석 결과에 대한 불확도 평가

김미주,지상운,황건중,이문수,조성일,Kim Mi-Ju,Ji Sang-Un,Hwang Keon-Joong,Lee Moon-Soo,Cho Sung-Eel 한국연초학회 2004 한국연초학회지 Vol.26 No.2

Uncertainty of final measurement results considering main uncertainty sources being in nicotine of mainstream smoke was estimated. This study was accomplished by using the ISO 'The Guide to the Expression of Uncertainty in Measurement'. Using the two point re-calibration method, uncertainty for nicotine concentration was calculated considering the uncertainty sources of each step. The concentration and uncertainty of nicotine in mainstream smoke was estimated as $153.95{\pm}17.84\;{\mu}g/mL\;(0.77\pm0.089 mg/cig)$. The expanded uncertainty was $17.84 {\mu}g/mL(\pm0.089 mg/cig).$ The reported expanded uncertainty of the measurement is stated as the standard uncertainty of measurement multiplied by a coverage factor of 2, which for a normal distribution corresponds to a coverage probability of approximately $95\%$ The former expression indicates the conversion concentration into the sample.

담배연기중 일산화질소의 신속분석

이문수,장기철,지상운,Rhee, Mun-Su,Jang, Gi-Cheol,Ji, Sang-Un 한국연초학회 1993 한국연초학회지 Vol.15 No.1

Nitric oxide of gas phase in cigarette smoke was conveniently determined by Chemiluminescence NO analyzer with 20 ports rotary type smoking machine Data are presented to support this observation. On the basis of this method the amount of NO in the smoke from nonblended and commercial cigarettes were estimated. Gas phase NO concentration in main stream smoke was correlated well with the nitrate levels in tobacco leaves, but there was no correlation with the tar yields. In consequence, it would be considered that the methodology in this study provided simple and rapid quantification for NO in cigarette smoke.

다채널 선형자동흡연장치 부착형 부류연 포집장치의 개발과 평가

김효근,황건중,지상운,이존태,이문수,Kim Hyo-Keum,Hwang Keon- Jung,Ji Sang-Un,Lee John-Tae,Rhee Moon-Soo 한국연초학회 2005 한국연초학회지 Vol.27 No.1

The Fishtail Chimney system mounted on 1 channel smoking machine is not appropriate for the routine analysis of sidestream smoke, because of its low repeatability and very long time required for smoke collection. To overcome this inconvenience, we developed a new sidestream smoke collecting apparatus compatible for 8 channel linear smoking machine. An electric motor driven stroke and automatic control system were adopted in this device to maximize convenience and efficiency of its operation. Also, we carried out the international collaborative study on monitoring sidestream smoke analysis to test the performance of this system. From the statistical analysis of the data obtained in our laboratory and other participating labs, it has been indicated that the newly developed sidestream smoke collection apparatus could be applicable to the routine analysis of sidestream smoke.