Aeromonas hydrophila 에 대한 비단잉어의 면역반응과 수산약제의 영향

지보영(Bo Young Ji),박수일(Soo Il Park) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

This study was carried out in order to investigate the immunosuppressive factor and immune response of color carp. The protection and serum antibody production of juvenile color carp aganist Aeromonas hydrophila were investigated on the effect of temperature differences and injected several aquatic drugs, i.e. Hydrocortisone, Oxytetracycline, Chloramphenicol and Ascorbic acid. The fish were injected intraperitoneally with 1㎎/fish of HKC and FKC at three different temperature conditions as 16℃, 24℃, and 32℃ respectively. There were induced better protection and serum antibody production in the fish which had been kept at 24℃ than in the fish which had been kept at 16℃ and 32℃. The FKC immunized fish were followed 24 hrs later with intraperitoneal injection of 40㎎/㎏ body weight of Hydrocortisone, 60㎎/㎏ body weight of Oxytetracycline. 60㎎/㎏ body weight of Chloramphenicol and 30㎎/㎏ body weight of Ascorbic acid, respectively. The control fish were injected PBS only. The fish given the above aquatic drugs reduced serum antibody production level and protection rate when compared to control fish. As the results, immune response of juvenile color carp immunized FKC at 24℃ was more effective than 16℃ or 32℃ and immune response of juvenile color carp injected several aquatic drugs which was seemed to be immunosuppressive factor.

생약재 황금 뿌리 열수추출물의 넙치 투여시 질병저항성에 미치는 영향

지보영 ( Bo Young Jee ),서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),이은혜 ( Eun Hye Lee*,),최희정 ( Hee Jung Choi ),김진도 ( Jin Do Kim ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.1

Effects of various concentration of skullcap Scutellaria baicalensis in the diets on a nonspecific immunity and a disease resistance of olive flounder were investigated. After feeding trial, weight gain of fish fed 0.05% skullcap immersed group was higher than that of fish fed 0, 0.1 and 1% skullcap diet but no significant differences were observed among the experimental groups. Furthermore, no significant differences in hematological indices of olive flounder were found among the experimental groups. Lysozyme activity in the serum and kidney of the administrated group(0.05% skullcap immersed group) was significantly higher than the control group. In addition, the chemiluminescent(CL) responses of head kidney leucocytes from the 0.05% skullcap immersed group was significantly higher(P<0.05) than the control group. In the histological results, the 1% skullcap immersed group appeared to have the detrimental effects for fish health. In a challenge experiment with Edwardsiella tarda(GY-01) and Streptococcus iniae(FT5228), relative percent survival (RPS) in the 0.05% skullcap immersed group was higher than the control group injected with E. tarda(GY-01) at 4th and 8th weeks. The results suggest that the skullcap extract (0.05%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen such as E. tarda.

담수산 백점충(Ichthyophthirius multifiliis)에 관한 연구 II. 백점충의 인위 감염 및 어체내 충체 발달상

지보영,김기홍,박수일,김이청,Ji, Bo-Young,Kim, Ki-Hong,Park, Soo-Il,Kim, Yi-Cheong 한국어병학회 1998 한국어병학회지 Vol.11 No.1

본 연구는 내수면 양식 어류에 백점충, Ichthyophthirius multifiliis를 인위 감염시켜 감염 특성 및 어체내 충체 발달상을 밝히고자 하였다. 어종별(무지개 송어, 메기, 이스라엘 잉어), 수온별($15^{\circ}C$, $18^{\circ}C$, $21^{\circ}C$) 및 충체 발달 단계별(trophont, protomont, theront) 인위 감염 시험 결과, 어종별로는 무지개 송어 시험구에서, 수온별로는 $18^{\circ}C$ 시험구에서 그리고 충체 발달 단계별로는 theront 시험구에서 양성적인 인위 감염을 확인할 수 있었다. 그리고 theront 농도별에 있어서는 어체당 1000개체의 theront가 감염을 양성적으로 유도할 수 있는 것으로 조사되었으며 어체당 1500개체 이상의 theront는 무지개 송어치어에 100%의 감염을 유도하였다. 수온 $18^{\circ}C$의 인위 감염된 무지개 송어에서 7일 동안 어체내 충체의 발달 과정을 조사한 결과, 충체는 시간이 경과함에 따라 연속적인 발달을 행하여 크기가 증대되었으며 감염 3일째부터는 성숙되는 것으로 확인되었다. 그리고 총체의 형태학적 변화는 감염 2일째에는 구부장치가 기능적으로 발달하기 시작한 것을 볼 수 있었고, 감염 4일째부터는 수축포가 현저히 발달되기 시작하였으며 감염 6일째는 이들의 수가 현저히 증가한 것을 확인할 수 있었다. 그리고 감염 기간이 경과함에 따라 충체는 숙주의 아가미 일차 새변의 입새 동맥쪽으로 이동하는 경향을 나타내었다. Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.

Negatively Charged Membrane을 이용한 해수 중어류질병바이러스의 검출

지보영 ( Bo Young Jee ),김광일 ( Kwang Li Kim ),이순정 ( Soon Jeong Lee ),김기홍 ( Ki Hong Kim ),진지웅 ( Ji Woong Jin ),정현도 ( Hyun Do Jeong ) 한국수산과학회(구 한국수산학회) 2013 한국수산과학회지 Vol.46 No.1

After an outbreak of viral disease in an aquafarm, release of virus(es)from infected fish into environmental seawa- ter has been suspected. ln the present study, we utilized a negatively charged membrane(HAtype)as an efficient method for concentration and detection of fish pathogonic viruses, specally, megalocytivinus and viral hemor- rhagic septicemia virus(VHSV)prent in field-collected seawater samples of inoculated into seawater artificially. Positivly charged viruses adsorbed onto the negatively charged membrane and were cluted with l mM NaOH (pH10.5)following rinsing with 0.5 mM H2SO4(pH3.0). Megalocytivirus and VHSV particles isolated using an- egatively changed HA membrane from seawater inoculated with each virus at a concentration of 10 viral paricles/ mL were of sufficient quantity to show positive results in atwo-step PCR(or RTtwo-step PCR); however, despite it being negatively charged, a cellulose acetata(CA)membraneshowed negative results. ln quantitative PCR, the detection limits of the HA membrane for megalocytivirus and VHSV in seawater were 1.20E+00 viral particles/ mL and 1.22E+01 Viralpartieles/mL, respectivly. The calculated mean recovery yields fiom 1 L seawater spiked with kmown concentrations of megalocytivirus and VHSV particles were 28.11% and 23.00%, respectively. The concentrate of a 1-L samplc of culturing seawater from the aqutank of flounder suffering from VHSV showed clear positive results in PCR when isolated with an HA, but not a CA, membrane, viral isolation using an HA membrane is a practical and reliable method for detection of fish pathogenic virtes in seawater.

수온변화에 따른 해수내 VHSV(Viral Hemorrhagic Septicemia Virus)의 생존능 분석

정지민(Ji Min JEONG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),서정수(Jung Soo SEO),황성돈(Seong Don HWANG),이지훈(Ji-Hoon LEE),황지연(Jee Youn HWANG) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.5

2005년부터 2007년 사이 양식 넙치, Paralichthys olivaceus를 대상으로 한 어류바이러스 검출에 대한 통계 자료

조미영 ( Mi Young Cho ),박경현 ( Gyeong Hyun Park ),지보영 ( Bo Young Ji ),김진우 ( Jin Woo Kim ) 한국어병학회 2010 한국어병학회지 Vol.23 No.2

The epidemiological study was performed to survey the prevalence of fish pathogens in cultured olive flounder, Paralichthys olivaceus from 2005 to 2007. In this study, the fish pathogens were detected from 1,528 among 2,238 fish samples collected yearly in 5 sites from February, May, August and November. Annual incidences for three years show a yearly increase and there were 60.6% in 2005, 66.7% in 2006 and 72.3% in 2007, respectively. Seasonal prevalence was 63.5% in February, 67.4% in May, 75.1% in August and 64.4% in November for three years. The detection rates of 6 viral pathogens were 35.6% in 2005, 44.6% in 2006 and 24.4% in 2007 and the peak rate was 55.4% at adult size group (above 41㎝). Viral nervous necrosis virus (24.7%) has been the most predominant virus in this investigation, while much lower rates were noted in viral haemorrhagic septicemia virus (10.6%) and red sea bream iridovirus (0.9%).

전복에 Oxytetracycline의 처리에 따른 잔류성 분석

김나영(Na-Young KIM),조희성(Hee-Sung JO),한지도(Ji-Do HAN),박민우(Min-Woo PARK),김진우(Jin-Woo KIM),김현정(Hyun-Jeong KIM),지보영(Bo-Young JEE),원경미(Kyoung-Mi WON) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.1

Two digenean parasites infestations of sea bass, Lateolabrax japonicus(Cuvier), form the Korean southern sea

김기홍,최은석,지보영,허성회,Kim, Ki-Hong,Choi, Eun-Seok,Ji, Bo-Young,Huh, Sung-Hoi 한국어병학회 1997 한국어병학회지 Vol.10 No.1

한국 남해안에서 채집된 농어의 장에서 흡충류 2종-Tergestia laticollis, Biovarium cryptocotyle-이 검출되었다. T. laticollis는 구흡반의 주위로 거꾸로된 왕관모양의 두엽들과 인두주변에 6쌍의 판자모양 구조물 및 충란의 크기 등에 의해 다른 종들과 구분되어졌으며, B. cryptocotyle의 가장 특징적인 점은 난소가 2분지된 점이었다. 이 두 종은 우리나라에서 처음 기록되는 미기록종들이었으며, 농어는 T.laticollis의 새로운 종숙주임이 금번 연구를 통해 밝혀졌다. Two species of digeneans, Tergestia laticollis and Biovarium cryptocotyle, were collected from the intestine of sea basses(Lateolabrax japonicus) captured at the Korean Southern Sea. T. laticollis was characterized by having cephalic lobes around oral sucker, six muscular flanges on each lateral surface at level of pharynx, and species-specific egg size. The most outstanding characteristic of B. cryptocotyle was two-divided ovary. These two digenean species were the first recording species in Korea and L. japonicus was the newly recording host for T. laticollis.

Amoxicillin trihydrate의 단독 경구투여 및 정맥투여에 따른 뱀장어, Anguilla japonica 체내 약물동태학적 특성

전은지 ( Eun Ji Jeon ),서정수 ( Jeong Soo Seo ),김진도 ( Jin Do Kim ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),황지연 ( Jee Youn Hwang ),박명애 ( Myoung Ae Park ),지보영 ( Bo Young Jee ),김진우 ( Jin Woo Kim ),김이청 ( Y 한국어병학회 2010 한국어병학회지 Vol.23 No.3

아목시실린(Amoxicillin trihydrate; Amox)을 뱀장어(평균 체중 220±10 g)에 1일 1회 경구투여(40 및 80 mg/kg body weight) 및 정맥투여(1 mg/kg)한 다음, 경시적(0시간∼720시간)으로 혈장내 Amox의 잔류농도를 분석하였다. 40 및 80 mg/kg 농도로 경구투여한 모든 시험구에서 투여 6시간째 각각 3.3±0.5 및 3.4±0.1 ㎍/㎖로 최대혈중농도를 나타내었다. Amox의 모든 시험구는 투여 720시간째 혈중에서 검출되지 않았다. Amox의 경구투여방법에 따른 뱀장어 체내 약물 혈중농도 측정결과를 바탕으로 WinNonlin program을 이용한 2-compartment model로 하여 Amox의 흡수, 배설, 반감기 등 약물동태학적 매개변수(parameter)를 조사하였다. 2-compartment model을 이용한 분석을 통하여 40 및 80 mg/kg Amox를 경구 투여한 경우, 혈장농도-시간곡선하 면적(AUC)은 각각 464 및 667 ㎍·h/㎖, 혈중최고농도의 도달시간(Tmax)은 2.1 및 3.6 hr, 혈중최고농도(Cmax)는 3.04 및 3.4 ㎕/㎖로 계산되었다. 1 mg/kg Amox을 정맥 투여한 경우, 혈장농도-시간곡선하 면적(AUC)은 748 ㎍·h/㎖, 혈중최고농도(Cmax)는 4.2 ㎕/㎖로 계산되었다. 1일 1회 단독으로 40 및 80 mg/kg Amox를 각각 경구 투여시의 뱀장어내 생체내이용율(F%; bioavailability)은 각각 1.6, 1.1%로 매우 낮게 나타났다. 이러한 결과는 아목시실린을 삼수화물 형태로 사용함에도 낮은 어류내 생체내이용율을 가진다는 사실을 알 수 있다. The pharmacokinetic properties of amoxicillin trihydrate (Amox) were studied after single oral administration and single intravenous injection to cultured eel, Anguilla japonica, respectively (average 220±10 g, 28±1℃). Plasma samples were taken at 3, 5, 10, 15, 24, 30, 48, 96 and 144 h post-dose. The kinetic profile of absorption, distribution and elimination of Amox in plasma were analyzed fitting to a two-compartment model by WinNonlin program. In oral dosage of 40 and 80 ㎎/㎏ body weight, the peak plasma concentrations of Amox, which attained at 3∼12 h post-dose, were 3.4 and 3.3 ㎍/㎖, respectively. In intravenous injection with 1 ㎎/㎏, the peak plasma concentrations of Amox, which attained at 9 h post-dose, was 7.2 ㎍/㎖. The following parmeters were calculated for a single oral dosage of 40 and 80 ㎎/㎏ body weight, respectively: AUC (the area under the concentration-time curve)〓 464 and 667 ㎍·h/㎖; Tmax (time for maximum concentration)〓 2.1 and 3.6 h; Cmax (maximum concentration)〓 3.04 and 3.4 ㎍/㎖. Following intravenous injection at 1 ㎎/㎏, this parameters were AUC= 748 ㎍·h/㎖; Cmax〓 4.2 ㎍/㎖. The apparent oral bioavailability at 40 and 80 ㎎/㎏ were 1.6 and 1.1%, respectively. Despite using the trihydrate form of amoxicillin, the oral bioavailability was low in eel.

The helminthic parasites of greenling, hexagrammos otakii Jordan et Starks, from the Korean southern sea

김기홍,허성회,지보영,Kim, Ki-Hong,Huh, Sung-Hoi,Ji, Bo-Young 한국어병학회 1997 한국어병학회지 Vol.10 No.2

A monogenean gill parasite, Prosomicrocotyla gotoi, and a digenean intestinal fluke. Opecoelus sphaericus, were found from greenilngs (Hexagrammos otakii) captured at the Korean southern sea. P. gotoi was characterized by having two separated frills of opisthaptor and distinguished from P. chirii in testis and clamp numbers. O. sphaericus had three pairs of finger-like marginal appendages on the ventral sucker and the parasite was distinguished from O. nipponicus in the location pattern of testes. These two helminth species were the first recording species in Korea. 한국 남해안에서 채집한 쥐노래미를 대상으로 기생성 연충류를 조사한 결과 아가미에 기생하는 단생흡충류, Prosomicrocotyla gotoi, 1종과 장관에 기생하는 흡충류, Opecoelus sphaericus, 1종이 발견되었다. 이 두종은 모두 우리나라에서는 처음 기록되는 미기록종들이었다. P. gotoi는 후고착반(opisthaptor)이 두갈래로 나뉘어진 frill형태에 의해 특징지워지며, 유사종인 P.chirii와는 고환과 clamp의 수에 의해 구별되었다. O. sphaericus는 복흡반의 가장자리에 3쌍의 손가락 모양 돌기가 나 있었으며, 유사종인 O. nipponicus와는 고환의 위치에 의해서 구별되어졌다.