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실크 피브로인을 함유한 비출혈용 복합 지혈제 개발에 대한 연구
문보미 ( Bo Mi Moon ),주형우 ( Hyung Woo Ju ),박현정 ( Hyun Jung Park ),이정민 ( Jung Min Lee ),이옥주 ( Ok Joo Lee ),박찬흠 ( Chan Hum Park ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.2s
Epistaxis is defined as active bleeding arising from the nasal mucosa. It constitutes one of the most common otolaryngology emergencies and can be severe or even fatal. Hemostasis is a process which causes bleeding to stop, meaning to keep blood within a damaged blood vessel which involves differential sequential events. This system in humans has been the most extensively researched. However, lot of research work is going on and lot more needs to be done to overcome the problem associated with this serious issue. This study was conducted to develop a new sponge type of biomaterial to be used for ideal hemostatic agent. The combination of three biomaterials; Silk fibroin (SF), Hyaluronic acid (HA), Gelatin (Gel) and Polyvinyl alcohol (PVA) as proposed hemostatic agent were tested against commercially available hemostatic pad (ChitoClot®). A comparative study of these silk based hemostasis agents designed in combination of SF/HA/PVA and SF/Gel/PVA sponges were tested on injured model of rats. Each sponge to be tested was made same size of (1×1 cm) then applied on top of injury site while applying a pressure of 5 N for 30 seconds. The In vivo experiments were carried by creating a proximal arterial injury in unilateral femoral arteries of 12 anesthetized SD rats. In conclusion, this study suggest a new developed silk fibroin-based sponges as hemostatic materials, which induced durable hemostasis and an increased blood clotting in animal studies. These initial studies explores the loopholes present in the existing ChitoClot® pad compared with that of fabricated silkbased sponges.
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사례 연구를 통한 BIM 전환설계 프로젝트의 설계오류 이슈 분석
김범준(Kim Beom Jun),주형우(Ju Hyung Woo),장문석(Jang Moon Seok),김병주(Kim Byeoung Ju),진상윤(Chin Sang Yoon) 한국BIM학회 2016 KIBIM Magazine Vol.6 No.2
Recently, the introduction and spread of BIM technology has been actively promoted at the domestic level and abroad. However, the BIM adoption and diffusion rate in the whole construction industry has been slower than expected, this is because they applied to construction projects in a way that does not meet the basic objective of BIM adoption. The objective of this paper is to derive a BIM adoption strategy that can provide benefits and efficiency from the design phase, by analyzing issues related to design errors identified in two real world projects based on the 2D to BIM conversion process. Types of issues, type distribution, and degree of BIM contribution to find issues were analyzed in a quantitative way, and then a BIM adoption strategy was derived. As a result, this paper identified that there obviously exists a limit in the 2D to BIM conversion process by repeating the problems that occur in the traditional 2D design process. Therefore, the authors of this paper insist that the design firms should adopt BIM-based design processes, fully, to get financial benefits as well as to improve the design quality.
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PCL/TiO₂ Nanoparticle 3차원 지지체 제조 및 특성 평가
김정호(Jun Ho Kim),이옥주(Ok Joo Lee),Faheem A Sheikh,주형우(Hyung Woo Ju),문보미(Bo Mi Moon),박현정(Hyun Jung Park),박찬흠(Chan Hum Park) 한국고분자학회 2014 폴리머 Vol.38 No.2
Polycaprolactone(PCL)은 생분해성 고분자로 인장강도, 신장률, 충격강도 등의 기계적 물성이 우수하다. TiO2(titanium dioxide) nanoparticle은 친수성으로 밀도가 높고 생체적합성이 우수하다. 본 연구에서는 PCL과 TiO2(titaniumdioxide) nanoparticle을 이용하여 salt-leaching방법으로 3차원 다공성 지지체를 제작하였다. 제작한 지지체를 FESEM,FTIR, TGA, 압축강도 측정 등을 통해 물성을 분석하였다. TiO2 nanoparticle에 의해 물흡수도와 팽윤도는 감소하였으나 압축강도는 증가하였다. CCK-8 assay를 통해 세포의 증식률을 확인한 결과, TiO2 nanoparticle에 의한세포 독성은 없는 것으로 확인되었다. 이러한 연구결과는 PCL/TiO2 nanoparticle 지지체의 생체재료로 사용가능성을 제시하였다. Polycaprolactone (PCL) is a synthetic biodegradable polymer with excellent mechanical properties. TiO2 (tita-nium dioxide) has a hydrophilic, high density and excellent biocompatibility. In this work, we produced three-dimensional porous scaffolds with PCL and TiO2 nanoparticles using a salt-leaching method. Physical properties of the scaffolds were analyzed by FE-SEM, FTIR, TGA and compressive strength. Interestingly, the addition of TiO2 nanoparticles decreased the water absorption and swelling ratio of the porous scaffolds. However, the compressive strength was increased by TiO2. CCK-8 assay, which is generally used for the analysis of cell growth, shows that TiO2 nanoparticles have no cytotoxicity. Taken together, we suggest that the PLC/TiO2-scaffold can be used for biomedical applications.
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초음파와 동결/융해에 의한 실크/PVA 하이드로젤의 제조 및 특성 평가
이옥주(Ok Joo Lee),김정호(Jung Ho Kim),주형우(Hyung Woo Ju),문보미(Bo Mi Moon),박현정(Hyun Jung Park),Faheem A Sheikh,박찬흠(Chan Hum Park) 한국고분자학회 2013 폴리머 Vol.37 No.6
실크 피브로인과 poly(vinyl alcohol)(PVA)는 뛰어난 생체적합성과 수용성을 가져 생체의학 분야에서 주목하는 재료이다. 본 연구에서는 실크 피브로인과 PVA를 초음파와 동결/융해 방법으로 드레싱제로서 사용 가능한 하이드로젤을 제조하고자 하였다. 실크와 PVA를 100/0, 75/25, 50/50, 25/75, 0/100 비율로 혼합하였다. 제작한 하이드로젤을 FE-SEM, TGA, FTIR, 압축 강도 측정 등을 통해 물성을 분석하였다. 실크/PVA 하이드로젤은 PVA 함량이 증가할수록 공극 크기와 팽윤도는 감소하였으며, 젤의 강도는 증가하였다. PVA를 첨가함으로써 실크의 기계적 물성이향상되는 것을 확인하였다. 본 연구에서 제조된 실크/PVA 하이드로젤은 드레싱제로서 사용 가능성을 제시하였다. Biomaterials like silk fibroin (SF) and poly(vinyl alcohol) (PVA) have received increasing attention in biomedical applications because of their attractive properties such as hydrophobicity and biocompatibility. In this study, efficient systems consisting of interpenetrating SF/PVA hydrogels were prepared as potential candidate for wound dressing applications. A simple approach consisting of sonication and a freezing-thawing technique was adopted to fabricate the hydrogels. Different blend ratios consisting of SF (100, 75, 50, 25 and 0%) with respect to the weight of PVA were prepared. The produced hydrogels were characterized for physico-chemical investigations using various states of techniques like; FE-SEM, TGA, FTIR and tensile strength. The addition of PVA to SF was proved to be beneficial in terms of reducing the pore size and swelling ratio of hydrogels. The mechanical property of SF had been increased by addition of PVA. These results show that SF/PVA hydrogels may serve as potential candidates for wound dressing application.
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