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두부비지의 버섯 폐배지 대체 수준이 발효사료의 영양소 함량, 미생물 성상 및 반추위 내 발효특성에 미치는 영향
주영호 ( Young-ho Joo ),정희한 ( Hui-han Jeong ),김동현 ( Dong-hyeon Kim ),이혁준 ( Hyuk-jun Lee ),이성신 ( Seong-shin Lee ),김상범 ( Sang-bum Kim ),김삼철 ( Sam-churl Kim ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.5
This study aimed to estimate the effects of replacing Mushroom By-Product (MBP) with Tofu By-Product (TBP) on the chemical composition, microbes, and rumen fermentation indices of Fermented Diets (FDs). The basal diet was formulated using MBP, TBP, rice bran, molasses, and inoculants. The MBP in the basal diet was replaced with TBP at 0, 5, and 10% on Dry Matter (DM) basis for the experimental diets. The experimental diets were fermented at 39°C for 144 h. Chemical composition, pH, microbes, and rumen fermentation indices of the FDs were analyzed. With increasing TBP replacement, crude protein content of FDs increased (L, P < 0.001), whereas crude ash content decreased (L, P = 0.002). Lactic acid bacteria and Bacillus subtilis contents in the TBP-replaced FDs were higher than those in the control (P < 0.05), whereas pH level and mold count were lower (P < 0.05). With increasing TBP replacement, in vitro rumen digestibility of DM (L, P = 0.053) and neutral detergent fiber (L, P = 0.024) increased, wheres rumen pH changed (P = 0.026) quadratically. Rumen total volatile fatty acid (L, P = 0.001) and iso-butyrate contents (Q, P = 0.003) increased with increasing TBP replacement. In conclusion, this study indicates that the replacement of MBP with TBP could improve the quality of FD.
한우분에 굴 패각분말을 첨가 시 분의 특성과 미생물에 미치는 영향 -실험실 연구를 중심으로-
장홍희,주영호,서명지,김지윤,이성신,최정석,정승민,노현탁,김삼철,Chang, Hong Hee,Joo, Young-Ho,Seo, Myeong-Ji,Kim, Ji-Yoon,Lee, Seong-Shin,Choi, Jeong-Seok,Jeong, Seung-Min,Noh, Hyeon-Tak,Kim, Sam-Churl 한국환경과학회 2021 한국환경과학회지 Vol.30 No.8
To improve the environmental management and resources, in this study, we aimed to investigate the effect of adding oyster shell powder to Hanwoo manure on its characteristics and microbial composition during the storage period. Additives were deposited on top of the manure surface at the rate of 0, 0.5, and 1% of oyster shell powder per 200 g of Hanwoo manure in a plastic container with three replicates; however, untreated manure litter served as the control. Manure characteristics (dry matter, organic matter and crude ash) and microbial composition (lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli) were evaluated at day 0, 2, 4, and 8. Manure characteristics exhibited an effect on dry matter, organic matter, and crude ash at day 2 and 8 (p<0.05), and not for day 0 and 4 (p>0.05). With the exception of yeast content at day 4 of storage, lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli exhibited no significant differences in all conditions during the storage period. Conclusively, addition of 1% oyster shell powder to Hanwoo manure resulted in slightly better manure characteristics; however, its microbial composition remained unchanged.
영남산간지역에서 옥수수와 수수×수수 교잡종의 파종시기와 수확시기가 조사료 생육 특성과 사료가치에 미치는 영향
이혁준,주영호,이성신,한옥규,구자환,민형규,오정식,김삼철,Lee, Hyuk-Jun,Joo, Young-Ho,Lee, Seong-Shin,Paradipta, Dimas Hand Vidya,Han, Ouk-Kyu,Ku, Ja-Hwan,Min, Hyeong-Gyu,Oh, Jung-Sik,Kim, Sam-Churl 한국초지조사료학회 2019 한국초지조사료학회지 Vol.39 No.2
본 연구는 영남산간지역에서 옥수수와 수수${\times}$수수 교잡종의 파종시기와 수확시기를 구명하고자 2015년부터 2016년까지 수행하였다. 옥수수는 국내 육성품종인 광평옥을 5월 8일, 19일 및 27일에 파종하였고 8월 10일, 20일 및 30일에 수확하였다. 수수${\times}$수수 교잡종은 수입품종인 SS405를 5월 27일, 6월 6일과 16일에 파종하였고 8월 10일, 20일 및 30일에 수확하였다. 옥수수의 생산성 측면에서는 파종시기가 빠를수록 간장, 생엽수, 이삭비율, 생초수량 및 건물수량이 증가하였으며, 수확시기가 빠를수록 건물수량은 감소하였다. 사료가치 측면에서는 파종시기가 빠를수록 건물과 조단백질 함량은 증가하였고, 수확시기가 빠를수록 NDF와 ADF 함량은 감소하였다. 수수${\times}$수수 교잡종의 생산성 측면에서는 파종시기가 빠를수록 초장, 생초수량 및 건물수량은 증가한 반면, 수확시기가 빠를수록 초장, 생초수량 및 건물수량은 감소하였다. 사료가치 측면에서는 파종시기가 빠를수록 건물 함량은 증가하였으나, NDF 함량은 감소하였다. 조단백질 함량은 수확시기가 빠를수록 증가하였다. 이상의 결과에서 영남산간지역에서 여름사료작물로 옥수수와 수수${\times}$수수 교잡종을 생산할 경우 6월 초순 이전까지는 파종하여 8월 중순 이후에 수확하는 것이 조사료 생산성과 사료가치의 향상에 유리할 것으로 판단되었다. This study was carried out from 2015 to 2016 to identify the suitable sowing and harvesting dates of summer crops in the mountain of Yeongnam, South Korea. The experimental design consisted of the different sowing and harvesting dates as follows: corn hybrid (Z. mays, Kwangpyeongok) of sowing (May 8, 19, and 27) and harvesting (August 10, 20, and 30); sorghum${\times}$sorghum hybrid (Sorghum bicolor ${\times}$ Sorghum bicolor, SS405) of sowing (May 27, June 19 and June 27) and harvesting (August 10, 20, and 30). In corn hybrid, ear rate and dry matter (DM) yield decreased (p<0.05) with the postponement of sowing date. Otherwise, ear rate and DM yield increased (p<0.05) with the postponement of harvesting date. Crude protein content decreased (p<0.05) with the postponement of sowing date, but neutral detergent fiber content increased (p<0.05). In sorghum${\times}$sorghum hybrid, plant length and DM yield with the postponement of harvesting date increased (p<0.05), while crude protein content with the postponement of harvesting date decreased (p<0.05). This study concluded that sowing corn hybrid in early May and sorghum${\times}$sorghum hybrid in early June then harvest in the middle of August was recommend to increase dry matter yield and feed value.
밤 발효사료 제조과정에서 미생물 첨가수준이 영양소 함량과 반추위 내 발효특성에 미치는 영향
김동현 ( Dong-hyeon Kim ),주영호 ( Young-ho Joo ),이혁준 ( Hyuk-jun Lee ),이성신 ( Seong-shin Lee ),( Dimas H. V. Paradhipta ),최낙진 ( Nag-jin Choi ),김삼철 ( Sam-churl Kim ) 한국환경과학회 2018 한국환경과학회지 Vol.27 No.5
This study aimed to estimate the effect of inoculant application level on chemical composition and bacterial count of fermented chestnut meal (FCM), and its rumen fermentation characteristics. The inoculant contained Lactobacillus acidophilus (1.2 x 10<sup>10</sup> cfu/g), Bacillus subtilis (2.1 x 10<sup>10</sup> cfu/g), and Saccharomyces cerevisiae (2.3 x 10<sup>10</sup> cfu/g). The chestnut meal mixed with molasses, double distilled water, and inoculant at 1 kg, 3 g, 480 mL, and 20 mL ratio for the basal chestnut meal diet. The double distilled water from basal chestnut meal diet was substituted with bacterial inoculant at a level of 0 (Control), 20 (Medium), and 40 mL (High) in the experimental diets. The mixed experimental diets were incubated at 39℃ for 7, 14, and 21 days, respectively. On 7 days of FCM incubation, the contents of crude protein (CP) (quadratic, P=0.043) and neutral detergent fiber (quadratic, P=0.071) decreased by increases of inoculant application levels, whereas bacterial count (quadratic, P=0.065) and rumen NH3-N (linear, P=0.063) increased. By increases of inoculant application levels on 14 days of FCM incubation, the increases were found on dry matter (DM) (quadratic, P=0.085), CP (quadratic, P=0.059), acid detergent fiber (quadratic, P=0.056), in vitro DM digestibility (linear, P=0.002), rumen total volatile fatty acid (VFA) (linear, P=0.057), and rumen iso-butyrate (linear, P=0.054). However, the decreases were found on bacterial count (linear, P=0.002), propionate (linear, P=0.099), and butyrate (quadratic, P=0.082). On 21 days of FCM incubation, in vitro DM digestibility (linear, P=0.002) and total VFA (linear, P=0.001) increased by increases of inoculant application levels, whereas the contents of CP (quadratic, P=0.034) and neutral detergent fiber (quadratic, P=0.047) decreased. These results indicate that the FCM with a medium level of inoculant application and 14 of fermentation had beneficial effects by increasing DM digestibility and rumen total VFA content, without altering bacterial count.
연구논문 : 혼합 생균제 급여가 한국 토종닭의 생산성과 가슴살의 지방산 조성에 미치는 영향
이혁준 ( Hyuk Jun Lee ),김동현 ( Dong Hyeon Kim ),주영호 ( Young Ho Joo ),윤희 ( Hee Yoon ),최인학 ( In Hag Choi ),김수기 ( Soo Ki Kim ),송인근 ( In Geun Song ),장인환 ( In Hwan Jang ),김삼철 ( Sam Churl Kim ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.3
This study aimed to investigate the effects of dietary supplementation of mixed probiotics on growth performance and fatty acid profiles of Korean native chicken and to provide information regarding producers. Ninety six Korean native chicken (48 males and 48 females, Hanhyup-3-ho) were allocated to 16 wire cages with 4 treatments, 4 replicates and 6 chicks (3 males and 3 females) per wire cage and fed one of four diets containing 0, 0.5, 1 and 1.5% mixed probiotics for 6 weeks. There were no differences among treatments in growth performance of Korean native chicken. For fatty acid profiles, no statistically differences in each fatty acid, total saturated fatty acid and unsaturated fatty acids were observed in all treatments. In the present study, irrespective of statistically differences, inclusion of mixed probiotics tended to improve growth performance, reduce saturated fatty acid and increase unsaturated fatty acids in breast muscles from Korean native chicken compared to controls.
혼합 생균제 급여가 거세한우의 성장, 혈액성상 및 육질에 미치는 영향
( Dimas H. V. Paradhipta ),전충환 ( Chung-hwan Jeon ),최인학 ( In-hag Choi ),이혁준 ( Hyuk-jun Lee ),주영호 ( Young-ho Joo ),이성신 ( Sung-shin Lee ),김동현 ( Dong-hyeon Kim ),김삼철 ( Sam-churl Kim ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.8
In this study, the effect of probiotic supplementation on growth performance, blood metabolites, and meat quality of Hanwoo steer was investigated. A total of 32 Hanwoo steers (15-17 months, average body weight 462±37.9 kg) were randomly allotted to 4 dietary treatments (0, 0.5, 1.0, and 1.5% mixed probiotics), with four Hanwoo steers per pen (two replicates per treatments), and reared for 12 months. There were no differences among treatments in growth performance of Hanwoo steer (P>0.05); however, feed intake decreased linearly with increasing levels of mixed probiotics. Growth hormone and Blood Urea Nitrogen (BUN) levels responded linearly with increasing levels of dietary mixed probiotics (P<0.05), but not insulin and blood glucose did not. In particular, total cholesterol was significantly lower for the 1% mixed probiotic treatment in comparison with that of the other treatments (P<0.05). The pH, Thiobarbituric Acid Reactive Substances (TBARS), cooking loss, and meat color were influenced by increasing levels of mixed probiotics (P<0.05), but the carcass characteristics and shear force were not. Regarding sensory evaluation, the addition of mixed probiotics resulted in significant difference in meat color, tenderness, aroma, off-flavor, juiciness, and marbling score, but not in overall acceptability. In addition, fatty acid profiles indicated no differences between control and mixed probiotic treatments. In conclusion, mixed probiotic treatment at 1% levels can enhance consumer preferences possibly by reducing cholesterol and TBARS.