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항원결합에서 항-Z-DNA 단클론항체 Z44의 재조합 중쇄 및 경쇄 가변영역의 역할
권명희,주민경,서정규,김형일 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3
It has long been known that both heavy(VH) and light chain variable region(VL) of antibody are involved in antigen binding. Recently, reports for anti-DNA Abs such as Z22 and Hed-10 have revealed that VH chain is more important than VL in recoginizing antigen. However, it is not certain yet whether the VH chain alone of these specific anti-DNA antibodies has dominant role or such dominance of VH chain can be applicable to other anti-DNA Abs. To investigate this possibility in connection with the role and contribution of VL chain, experiments were performed using mAb Z44 which binds to Z-DNA but rocognizes different epitopes from Z22 which share 95% sequence homology. The recombinant proteins including Z44VH and Z44VL were produced by E.coli. After purification of the these antibodies, the affinity and specificity of recombinant Ab proteins to DNA antigens were tested. The results were as follows; Z44VH bound most strongly to Z-DNA and bound also to ssDNA, whereas Z44VL did not bind to B-DNA, ssDNA, and Z-DNA. In vitro association of Z44VH with Z44VL has increased the affinity to Z-DNA synergistically compared with Z44VVH alone. In the case of association of Z22VH with Z22VL affinity to Z-DNA has showed synergistic increment compared with Z22VH alone. However, the associations of Z44VH with Z22VL or Z22VH with Z44VL did not show any significant increments of affinity. From our findings that Z44VH alone could bind to Z-DNA or to ssDNA and that Z 44VL could not, it could be concluded that Z44VH has dominant role in recognizing the antigen. In addition, it may be suggested that Z44VL can increase the affinity of Z44VH and play some role in changing the specificity of Z44VH.
홍현주,차민호,최강,황선희,소유경,주민경,남궁기,정재승 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1
Objectives : Tourette's Disorder (TD) is a chronic neuropsychiathc disorder characterized by multiple motor and vocal tics with onset in childhood. The aim of this study was to ascertain the increased cortical information transmission in frontal area during tic suppression in drug naive boys with TD using new nonlinear analysis of EEGs, be called Transfer Entropy (TE) which can detect the directed exchange of information between two systems. Methods : Subjects were 11 drug naive boys with DSM-IV diagnosis of TD and 10 control boys. Clinical assessments were performed, and EEGs were recorded from 19 scalp loci of the international 10-20 systems. TE was estimated by EEG time-series data after noise reduction. TE difference between TD and control during resting state and between tic suppression and resting state in TD were investigated. Rcsults : Elevated TE was found in extensive channels, including frontal, central and temporal channels (F7, Fz, F8,Cz, C3, P3, T3, and T4) in resting state ofTourette's disorder compared to normal controls. Duhng tic suppression elevated TE was found in more extensive and asymmetrical channels especially prefrontal area (Fpl, Fp2, F3, Fz, F7, F8, Cz, C4, C5, T3, and T4). Conclusion : These findings suggest that pathogenesis of Tourette's disorder involve impaired cortical neuronal modulation in subcortical neural circuits. EEG analysis of TE may be a useful tool to investigation of cortical mechanism of psychiatric illness.