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고정삼(Jeong Sam Koh),좌창숙(Chang Sook Jwa),김영휘(Yong Whee Kim) 한국응용생명화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.2
Quality characteristics of Satsuma mandarin (Citrus unshiu Marc. var. okitsu) harvested in early December according to production areas and sea level altitude of Cheju were investigated. Soluble solids and flesh ratio were 9.06∼12.98, 77.32∼81.27% on citrus fruits harvested in orchards located below 100 m sea level (lower area), and were 10.16∼12.26, 75.00∼81.55% in orchards 150 m sea level altitude (higher area), respectively. Acid content of citrus juice was 0.83∼1.21% in fruits harvested in lower area, and was 0.94∼1.34% in higher area except Hankyung-myun area. The differences of soluble solids and acid contents by harvesting periods for 10 days were 0.91 and 0.82 for soluble solids; 0.15 and 0.13% for acid contents, respectively. In proportion to late harvesting, Brix/acid ratio and fruit quality were improving. Soluble solids of fruits harvested from lower area were higher than those of fruits from higher area, but acid contents were lower in fruits from lower area than in fruits from higher area. In sensory evaluation on fruit appearence, the differences between two altitudes were not clear, but panelists marked higher points on fruits harvested in main production areas, Seogwipo-si and Namwon-cup which are southern areas. Fruit quality harvested in Seogwipo-si areas showed better quality than fruits from other harvested areas.
제주지역 무에서 벼룩잎벌레와 무잎벌의 색트랩과 집합페로몬에 대한 반응과 연중 발생특성
송정흡,양영택,양철준,최병렬,좌창숙,Song, Jeong Heub,Yang, Young Taek,Yang, Cheol Jun,Choi, Byeong Ryul,Jwa, Chang Sook 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.4
벼룩잎벌레(Phyllotreta striolata)와 무잎벌(Athalia rosae ruficornis)는 제주지역 무에서 돌발적으로 발생하여 피해를 주는 해충이다. 본 연구는 벼룩잎벌레와 무잎벌 성충의 트랩 색(황색과 청색)과 벼룩잎벌레 집합페로몬(+)-(6R,7S)-himachala-9,11-diene과 기주식물 휘발성 물질인 allyl isothiocyanate의 혼합물을 이용 두 해충의 발생을 간편하게 예찰하는 방법을 개발하기 위하여 수행하였다. 벼룩잎벌레는 트랩 색에 관계없이 집합페로몬이 부착된 점착트랩에 더 많은 성충이 유인되었으며, 무잎벌은 황색점착트랩이 청색트랩보다 더 많이 유인되었고 벼룩잎벌레 집합페로몬은 유인량에 관계가 없었다. 따라서 벼룩잎벌레 집합페로몬을 부착한 황색점착트랩을 이용하면 벼룩잎벌레와 무잎벌 성충 발생을 효율적으로 예찰할 수 있었다. 점착트랩의 높이는 기주식물에 가까울수록 두 해충의 유인수가 많아 기주식물 상단 10 cm 높이로 설치하는 것이 좋을 것으로 판단되었다. 벼룩잎벌레와 무잎벌의 연간 발생피크는 각각 3회와 5회정도 나타났으며, 두 해충의 첫 주발생시기는 벼룩잎벌레는 3월중하순, 무잎벌은 4월 중하순이었다. 벼룩잎벌레와 무잎벌 성충의 연간 발생특성을 이용하면 두 해충의 무에서의 피해를 최소화하는데 중요한 정보로 활용될 것으로 기대된다. Striped flea beetle, Phyllotreta striolata (SFB) and turnip sawfly, Athalia rosae ruficornis (TSF) are two economically important sporadic pests in radish fields on Jeju island. The response of adult SFB and TSF to a mixture of aggregation pheromone, (+)-(6R,7S)-himachala-9,11-diene and host plant volatile, allyl isothiocyanate (HAI), as well as to yellow and blue sticky traps was examined in radish fields. Adult SFB was more attracted to the sticky trap with HAI, regardless of the color of the sticky trap; however, adult TSF was more attracted on the yellow sticky trap than blue, and no effect of HAI was observed. The adult SFB and TSF can be effectively monitored using yellow sticky traps placed 10 cm above the plant canopy. SFB and TSF had 3 and 5 peak times in a year, respectively. The first peak occurred in the middle of March for SFB and mid-late of April for TSF. We expect that the results of the present study can facilitate minimizing the damage caused by the two important pests in commercial radish fields.
고정삼,양상호,양영택,좌창숙 ( Jeong Sam Koh,Sang Ho Yang,Young Tack Yang,Chang Sook Jwa ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
In order to determine the optimmum harvest time of Citrus unshiu Marc. var. miyagawa and C. unshiu Marc. var. okitsu produced in Cheju, citrus fruits sampled at packing houses or harvested directly on citrus tree in south and north area of Cheju were analyzed. The fruits were grown in size till middle of October, and soluble solids were increased continuously after that. The fruits size were different by positional directions on the tree, the quality of citrus fruits in central southern positions on the tree was good for fresh fruit consumptions. Compared with only the quality of citrus fruits as a factor of soluble solids, total sugar, pH, and color index, the optimum harvest time were supposed to be reasonable at late of November for C. unshiu Marc. var. okitsu, and at early of December for C. unshiu Marc. var. miyagawa. The results obtained from citrus fruits sampled at packing houses were supposed to be not suitable for determing the optimum harvest time, because of storage after harvest at ordinary harvesting time.
시설딸기 주요 비행해충의 황색끈끈이트랩 이용 효율적인 발생예찰
양철준,송정흡,양영택,김효정,송민아,좌창숙,Yang, Cheol Jun,Song, Jeong Heub,Yang, Young Taek,Kim, Hyo Jeong,Song, Min A,Jwa, Chang Sook 한국응용곤충학회 2017 한국응용곤충학회지 Vol.56 No.3
시설딸기 주요 비행 해충인 꽃노랑총채벌레 Frankliniella occidentalis와 목화진딧물 Aphis gossypii 유시충, 온실가루이 Trialeurodes vaporarium에 대해 발생초기 파악을 위한 황색끈끈이트랩 이용기술을 규명하였다. 제주지역 농가 재배 3개소에서 5개년 동안(2013~2017년) 재배 전 기간(9월~이듬해 5월)에 걸쳐 조사한 트랩자료를 이용 분석하였다. 해충의 공간분포 특성은 일반적으로 이용하고 있는 Taylor's power law와 Iwao's patchiness regression으로 분석한 결과 대상 해충 모두 집중분포를 하고 있었다. 두 회귀식 중 꽃노랑총채벌레와 목화진딧물 유시충은 Taylor's power law이, 온실가루이는 Iwao's patchiness regression이 공간분포 특성을 잘 설명하고 있었다. 트랩당 평균밀도와 최고밀도, 11마리 이상 잡힌 트랩 비율 간에는 높은 상관관계를 갖고 있었다. 트랩당 평균 밀도 4.0마리를 추정에 필요한 최소 트랩수는 꽃노랑총채벌레 13개, 목화진딧물 유시충 11개, 온실가루이 10개이었다. 고정 정확도 0.25 수준에서 3종 해충의 Taylor's power law과 Iwao's patchiness regression 상수를 이용하여 축차표본조사 중지선을 구하였다. Sampling plan using yellow sticky traps for the major strawberry flying pests - western flower thrips Frankliniella occidentalis adults, cotton aphid Aphis gossypii alate and greenhouse whitefly Trialeurodes vaporarium adults was developed to determine the initial occurrence time. The analyzed trap data were obtained from three commercial strawberry greenhouses for the whole growing season (September to May of the following year) during 2013 to 2017 in Jeju province. Three flying pests showed the aggregated distribution patterns resulted from commonly used regression techniques - Taylor's power law and Iwao's patchiness regression. Taylor's power law was better description of mean-variance relationship of the western flower thrips and the cotton aphid than Iwao's patchiness regression, otherwise greenhouse whitefly was better described by Iwao's patchiness regression. There were highly significant correlated among mean density per trap, maximum density and proportion of traps with more than 10 individuals. To estimate 4.0 heads of mean density per trap, the minimum number of traps were required 13 traps for western flower thrips, 11 traps for cotton aphid and 10 traps for greenhouse whitefly. The sequential sampling plans at the fixed precision level 0.25 were developed using parameters of Taylor's power law for western flower thrips and cotton aphid, and of Iwao's patchiness regression for greenhouse whitefly.
광합성세균 Rhodopseudomonas palustis 분리 및 IAA와 Carotenoid 생성에 관한 연구
김유경,조영윤,강호준,김정선,양성년,좌창숙,Kim, Yu-Kyoung,Cho, Young-Yun,Kang, Ho-Jun,Kim, Jung-Sun,Yang, Sung-Nyun,Jwa, Chang-sook 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.4
본 연구에서는 제주도 한라산 중산간 습지대 28개소에서 IAA 및 carotenoid 생성능이 우수한 광합성 세균 1종을 최종 선발하였으며, 16S rRNA 염기서열 분석과 생리학적 특성을 조사한 결과 Rhodopseudomonas palustris JK-1 균주로 동정하였다. JK-1 균주의 최적배양조건을 선발하기 위하여 pH, 온도, 빛 및 통기 등을 포함한 여러 가지 요인들이 균주의 생장과 IAA 및 carotenoid 등 광합성색소 생성에 미치는 영향을 조사하였다. 시험결과 JK-1 균주는 명/혐기조건에서 균생장, IAA 및 광합성색소 생성이 양호하였으며 암/혐기조건에서 균생장, IAA 및 광합성색소 형성이 모두 크게 억제되었다. 명/혐기조건에서 균생장, IAA 및 carotenoid 등 광합성색소의 생산을 위한 최적 pH, 온도 및 배양회전속도는 각각 7, $30^{\circ}C$, 150 rpm, 9, $30^{\circ}C$ 및 150 rpm, 그리고 6, $25^{\circ}C$ 및 50 rpm이었다. 그리고 명/호기조건(0.5~1 vvm)에서는 명/혐기조건(0 vvm)보다 균생장 및 IAA 합성이 양호하였으나 광합성색소 형성은 크게 억제되었다. 따라서 최적배양조건은 명/혐기조건에서 pH 7, 온도 $30^{\circ}C$ 및 회전속도 100 rpm을 선발하였으며, IAA 합성을 유도한 배양액을 생육촉진 효과검증에 이용하였다. 시험결과 R. palustris JK-1 균주를 열무에 3% (v/v) 처리 시 지상부 및 지하부 건물중이 무처리 및 배지처리구 대비 각각 20~58% 및 40-28% 증가하였다. The JK-1 isolate which was the best producer of indole-3-acetic acid and carotenoid among the 388 strains isolated from 28 wetlands in Jeju, was identified to be Rhodopseudomonas palustirs belongs to a typical group of non sulfur purple bacteria based on 16S sRNA sequencing. This study investigated the effect of different cultural conditions of pH, temperature, agitation, light and aeration on growth, IAA and carotenoid production of photosynthetic bacterium JK-1 for optimization of IAA and carotenoid production. It was found that growth, IAA, carotenoid, and bacteriochlorophyll production with light (3,000~3,500 Lux) and agitation (100 rpm) showed better results than those with dark/static or dark/agitation (100 rpm) in anaerobic conditions. The optimal pH, temperature and agitation speed for cell growth were 7, $30^{\circ}C$, 150 rpm, for IAA production were 9, $30^{\circ}C$, 150rpm and for carotenoid production were 6, $25^{\circ}C$, 50 rpm, cultured for 72 h under anaerobic light, respectively. The growth and IAA production were high in aerobic culture compared with anaerocic culture, whereas carotenoid and bacteriochlorophyll content were decreased extremely in aerobic condition (0.5~1 vvm). Subsequently, the optimal culture conditions for JK-1 were selected with pH 7, $30^{\circ}C$ and 100 rpm under anaerobic light and the effect on plant growth was tested by pot assay. Inoculation of JK-1 with 3% (v/v) level caused increase in shoot and root dry weigh that varied from 20%~58% to 40%~28% in young radish in camparison to uninoculated treatment at 50 days of growth. The study suggests that the JK-1 isolate may serve as efficient biofertilizer inoculants to promote plant growth.