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Cholera菌의 重金屬Ion反應에 미치는 培地와 菌處置의 影響
曺喜滿 中央醫學社 1971 中央醫學 Vol.21 No.3
S-, SR-, and R-forms of Vibrio cholerae biotype El Tor strain Ogawa 41 were obtained after culturing the organism successively in immune serum-containing broth. Influences of various culture media on the agglutination behaviour of the organisms were examined, using acrifiavine, lead acetate and manganous chloride as reacting agents. Glucose (1%) had an effect of moderately diminishing the agglutination intensity and titer of SR-form with lead acetate, while lactose (1%) had the contrary effect, i. e., even SR-form becoming agglutinable in manganous chloride solution. Trypsin treatment of the cells also brought about another interesting phenomenon; the differences between form variants in the agglutination degree with lead acetate were considerably dulled, even S-form becoming agglutinable while R-form loosing its spontanous agglutinability.
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