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한약재중의 잔류농약 분석 - GC에 의한 18종 잔류농약의 분석 -
황인숙,최병현,배청호,김명희,조해전,Hwang, In-Sook,Choi, Byung-Hyun,Bae, Chong-Ho,Kim, Myung-Hee,Cho, Hae-Jeon 한국생약학회 2001 생약학회지 Vol.32 No.3
This study was carried out to determine the 11 organochlorine, 7 organophosphorus residual pesticides in 251 crude drugs. These residual pesticides in herbal drugs were extracted with acetonitrile and the extracts were cleaned up via LC-florisil solid phase extraction column. The prepared samples were assayed for pesticide residues using GC-ECD, NPD with capillary column and identified by GC-MSD. Recoveries were $63.9{\sim}111.5%$ in the organochlorine pesticides and $69.8{\sim}92.4%$ in the organophosphorus pesticides, and detection limits were $0.001{\sim}0.65\;ppm$ in the organochlorine pesticides and $0.0009{\sim}0.0074\;ppm$ in the organophosphorus pesticides. Pesticide residues were detected in 9 cases.
황련과 감초의 수용성 혼합물로 부터 얻어진 반응침전물의 세포독성
은재순(Jae Soon Eun),조해전(Hae Jeon Cho),양재헌(Jae Heon Yang),전훈(Hoon Jeon),김영안(Young Ahn Kim) 한국생약학회 1995 생약학회지 Vol.25 No.4
The purpose of this research was to investigate the effect of reactionprecipitate from Coptidis Rhizoma and Glycyrrhizae Radix aqueous mixture(CGP; on the cytotoxicity. The effects of CGP on the growth of tumor cells, Balb/c 3T3 cell, mouse spleen cell and human lymphocyte were compared with those of berberine, glycyrrhizin and berberine glycyrrhizinate(BG), which were estimated by MTT colorimetric assay or cell counting. CGP, benberine and BG inhibited the growth of several tumor cells, such as Hep G2, A549, Raji, MCF-7, HeLa and KHOS-NP. Whereas, glycyrrhizin inhibited the growth of Raji and MCF-7, CGP did not affect on Balb/C 3T3 cells, mouse spleen cells and human lymphocyte at 10<sup>-6</sup>∼10^(-5)g/㎖. CGP increased the number of leukocyte in mice. This results indicate that CGP have the inhibitory action of the growth of human tumor cells, and the side effect of CGP is less than berberine and BG.