이산화티탄 분체의 광학적 특성과 자외선 차단효과

조춘구,권혜진,안선례 한국피부과학연구원 2011 대한피부미용학회지 Vol.9 No.2

In order to develop a new ultraviolet ray blocking material that will protect the skin from ultraviolet rays that are known to be the primary cause of skin aging and complement the weakness of existing ultraviolet ray scattering agents that had to be used in large amounts, the optical characteristics of titanium dioxide powder which is the major material of ultraviolet ray scattering agents relative to its shapes were analyzed and the correlations between the characteristics and the effect to block ultraviolet rays were examined. The optical characteristics of individual powder types of four different titanium dioxide products currently commercialized were analyzed. Then, W/O emulsions mixed with titanium dioxide in a 5% weight ratio were made and their in vitro SPF and PA values were measured. Among the four types of titanium dioxide with different shapes, ST-485 showed the highest UV Absorbance value in the area of ultraviolet rays followed by ST-455, ST-495 and P-25 in order of precedence and in the case of UV Reflectance values, ST-495 showed the highest value followed by P-25, ST485 and ST-455. In addition, it could be seen that when the UV Absorbance value of the powder was higher, the sun protection factor (SPF) value of the powder was also higher and that when the UV Reflectance value of the powder was higher, the protection grade of UVA (PA) of the powder was also higher.

달팽이 발효 추출물을 이용한 마스크 팩의 피부개선효과

조춘구,이민희,김인영,신지영 한국유화학회 2016 한국응용과학기술학회지 Vol.33 No.1

본 연구는 2단계 발효된 달팽이 추출물을 5% 사용한 시트 마스크 팩 (2F-SEM)을 개발하였 다. 이에 대한 피부미용학적 연구를 수행하기 위하여 순면 100%의 시트를 사용하여 얼굴모양으로 커팅 하여 사용하였다. 플라세보 마스크 팩 (placebo mask pack; PM)과 일반 용매추출에 의한 달팽이 추출 물을 사용한 마스크 팩 (Gene-SEM)에 대하여 피부 개선효과를 측정한 결과를 보고한다. 첫째; 2F-SEM의 보습효과는 PM 보다 11%, Gene-SEM는 PM 보다 4.7%가 상승되었다. 둘째; 2차 발효달 팽이 추출물이 함유된 마스크 팩의 탄력도는 PM 보다 13.8%, Gene-SEM PM 보다 6.7% 이상 개선하 는 효과를 보였다. 셋째; 2F-SEM의 피부 거칠기는 PM 보다 6.80%, Gene-SEM는 PM 보다 2.3%가 개선되었다. 넷째; 2F-SEM의 멜라닌감소효과는 PM 보다 15.0%, Gene-SEM는 PM 보다 8.7%이상 개선되었다. 다섯째; 2F-SEM의 잔주름개선효과는 PM보다 8.0%정도 우수하였다. Gene-SEM는 PM 보다 5.1%이상 개선되었다. 여섯째; 2차 발효 물을 이용한 2F-SEM의 관능 평가는 부드러움(softness), 보습 감(moisture), 주름개선효과(fine wrinkle improvement)에서 유의 차 있는 사용감촉을 보였다. This study related to get skin improvement effect of sheet mask pack using 5% of 2nd fermented snail extract. To the scientific study of the skin, it was used for this sheet mask of 100% cotton as cutting face shape. We reported the results of measurement of skin improvement effect with the placebo and Gene-SEM (general snail extract mask). First; moisturizing effect 2F-SEM (2nd fermented snail extract mask) has been increased about 11% than the PM and Gene-SEM was improved about 4.7% more than the PM (placebo mask). Second; elasticity of the mask pack containing 2ndfermented snail extract was 13.8% better than PM and Gene-SEM was improved about 6.7 % more than PM. Third; Skin roughness of 2F-SEM has improved 6.80% than PM and Gene-SEM was improved about 2.3% more than PM. Fourth; melanin reductive effect of 2F-SEM was improved about15.0% more than PM and Gene-SEM was improved about 8.7% more than PM. Fifth; fine wrinkle decline effect of 2F-SEM was about 8.0% better than PM and Gene-SEM was improved with 5.1% more than PM. Sixth; sensorial evaluation of 2F-SEM using 2nd fermented snail extract was significantly showed difference merits regarding softness, moisture, fine-wrinkle improvement.

노루궁뎅이버섯 균사체를 이용한 2 차 발효달팽이 추출물의 항노화 활성에 관한 연구

조춘구,이민희 한국유화학회 2016 한국응용과학기술학회지 Vol.33 No.1

본 연구는 달팽이육질을 2차 발효공정을 통하여 얻어진 추출물을 개발하여, 피부미용학적 항 노화 활성에 관한 것이다. 1차 발효물을 얻기 위하여 노루궁뎅이버섯균사체로 배양하였으며, 2차 유산균 발효공정을 통하여 2차 발효달팽이 추출물을 얻는 과정에 대하여 상세히 기술하였다. 이 연구에 적용된 균사체는 노루궁뎅이버섯균사체 (Hericium erinaceum Mycelium)와 유산균 (Leuconostoc mesenteroides) 발효균을 사용하여 추출하였다. 추출물의 최종 수율은 62wt%이었다. 2차 발효된 달팽이 추출물의 수분 32wt%, 아미노산 단백질류 31.5wt%, 다당체 15.7wt%, 지방산 12.3wt%, 기타성분이 8.5wt%가 함유하였다. 또한 피부미용학적 이론과 피부의 항노화에 관여하는지를 알아보기 위하여 DPPH에의한 항산화 활성, 엘라스틴의 효소(elastase)감소효과, tyrosinase저해율, fibroblast의 성장율, 콜 라겐합성률에 대하여 평가한 결과를 하기와 같이 보고한다. 첫째; 2차 발효달팽이 추출물의 항산화 효과 (DPPH, IC50%)는 7.27 mg/mL로, 비교군인 녹차추출물 11.8 mg/mL, 일반달팽이추출물 15.7 mg/mL, DL-a-토코페롤 9.25 mg/mL가 소요되었다. 둘째; 2차발효달팽이추출물의 엘라스틴 효소(elastase)의 발 현억제능(IC50%)은 32.5 mg/mL로 비교군인 녹차추출물 45.9 mg/mL, 일반 달팽이추출물 67.7 mg/mL가 소요되었다. 셋째; 2차 발효달팽이 추출물의 tyrosinase의 발현억제능(IC50%)은 140.3 mg/mL로 비교군인 녹차추출물 250.7 mg/mL, 일반달팽이추출물 389.5 mg/mL, 니아신아마이드 125.9 mg/mL가 소요되었다. 넷째; 2차 발효달팽이 추출물의 fibroblast의 성장률은 125.6%로 비교군인 녹차 추출물 98.9%, 일반 달팽이추출물 109.5%, DL-a-토코페롤 96.2%의 활성력을 보였다. 다섯째; 2차 발 효달팽이 추출물의 collagen생합성률은 118%로 비교군인 녹차추출물 87.3%, 일반달팽이추출물 93.2%, 아데노신 127.9%의 성장률을 보였다. 결론적으로 이 연구를 바탕으로 하여 미래에는 피부미용학적 활용 과 더불어 한국적 스킨케어 화장료 개발에 응용이 가능할 것으로 기대한다. This study is related to develop a snail extract through a snail secondary fermentation process, getting anti-aging activity with healthy and beauty skin care scientific applications. In order to obtain a primary fermentation was incubated with Hericium erinaceus mycelium. Through the secondary fermentation process using Leuconostoc mesenteroides, was deeply described a total process of obtaining second fermented extract using snail body. Mycelium is applied in this study was extracted using Hericium erinaceus mycelium and Leuconostoc mesenteroides. The final yield of the extract was 62 wt%. Experimental results of secondary fermentation snail extract were contained with 32 wt% water, 31.5 wt% total amino acid protein, 15.7 wt% polysaccharide, 12.3 wt% fatty acid and others 8.5 wt%. In addition, in order to study about skin beauty care and anti-aging activity, we evaluated antioxidant activity with DPPH, elastin enzyme (elastase) inhibitory activity, tyrosinase inhibition rate, collagen synthetic function, fibroblast synthetic activity. First; anti-oxidative activity of secondary fermentation snail extract (IC50%) was spent with 7.27 mg/mL, control samples were spent with green tea extract was 11.8 mg/mL, common snails extract was 15.7 mg/mL, DL-a-tocopherol was 9.25 mg/mL respectively. Second; elastin enzyme inhibitory activity of secondary fermentation snail extract (IC50%) was spent with 32.5 mg/mL, control samples were also spent with green tea extract was 45.9 mg/mL, general snail extract was 67.7 mg/mL. Third; tyrosinase inhibitory activity of secondary fermentation snail extract (IC50%) was spent with 140.3 mg/mL, control samples were also spent with green tea extract was 250.7 mg/mL, general snails extract was 389.5 mg/mL, niacineamide was 125.9 mg/mL. Forth; fibroblast synthetic activity of secondary fermentation snail extract was increased with 125.6%, control samples were also spent with green tea extract was 98.9%, general snails extract was 109.5%, niacineamide was 125.9 mg/mL, DL-a-tocopherol was 96.2%. Fifth; collagen synthetic activity of secondary fermentation snail extract was increased with 118%, control samples were also spent with green tea extract was 87.3%, general snails extract was 93.2%, adenosine was 127.9%. In conclusion, on the basis of this study, in the future it is expected to be applied to the skin beauty care application and development of Korean style cosmetic products.

Inositol-Hexaphosphoric Acid의 피부노화방지

조춘구,홍세흠 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

The anti-skinaging effects on woman's skin were investigated with inositol-hexaphosphoric acid(IP_6). The concentrations (wt%) of IP_6 0, 0.1, 0.2, 0.4, 0.8, 1.0. The IP_6-skin lotions were applied on the arms and face of the subjects everyday for 7 weeks. The moisture, lightening, sebum, elasticity and wrinkle of the skin were measured. The experimental instruments were corneometer, sebumeter, autometer, mexameter. MX-16 and image analyzer. It showed that IP_6 substantially improved the subject's skin condition. The moisture value changed from 47 to 89, the sebum values of dry and oily skins changed close to that of normal skins, lightening of the skin changed from 490 to 461, elasticity changed from 0.118 to 0.134, and the improvement on wrinkle was 12.9%. With the experimental results the 0.4-IP_6 skin lotion showed the best results.

Antioxidative and Antimicrobial Effects to Skin Flora of Extracts from Peel of Allium cepa L.

조춘구,권혜진,안선례 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.3

In this paper the experiments for the antioxidative and antimicrobial extracts from the peel of Allium cepa L. were carried out in order to research the possibility of natural source development in the cosmetic industry. After drying extracts from the peel of Allium cepa L. the extracts were obtained at 40℃ and 80℃by using 95% ethanol as solvent and 40℃ and 100℃ by using distilled water as solvent, respectively. The contents of polyphenol and flavonoid obtained from the four extracts were analyzed. The restraining effect of lipid oxidations for the extracts from Allium cepa L. peel in 95% ethanol at 80℃ showed more excellent than that of BHT which was used as the control group. Also the free-radical removing effect showed that the extracts from Allium cepa L. peel in 95% ethanol at 80℃ in 5000ppm had the highest antioxidative effect as 60.19%. As to the skin floras, the antimicrobial effects were measured using gram-positive bacteria such as Propioni bacterium acne (KCTC 3320), Propioni bacterium acne (KCTC 3314), Staphylococcus epidermidis (KCTC 1917), Staphylococcus aureus (KCTC 1927), Bacillus subtilis (ATCC 6633) and gram negatives bacteria such as Pseudomonas aeruginosa (ATCC 8739), Escherichia coli (ATCC 9027) and Pityrosporium ovale (KCCM 11894). The antimicrobial effect by the disk paper method showed antimicrobial activity in every bacteria, and the extracts from Allium cepa L. peel in 95% ethanol at 40℃ showed the highest effect. As a result of analyzing antimicrobial activity by the optical density method, the extracts from Allium cepa L. peel in 95% ethanol at 40℃ showed a higher growth inhibition rate than the extracts from Allium cepa L. flesh in every bacteria. From the result the extracts from peel of Allium cepa L. showed excellent antioxidative effects and antimicrobial effects to skin flora.

Oil의 구조와 Microemulsion 생성영역

조춘구 崇實大學校 大學院 1993 崇實大學校 大學院 論文集 Vol.11 No.-

누에추출물의 지방산 성분 분석

조춘구,홍세흠,류강선 한국피부미용향장학회 2012 한국피부미용향장학회지 Vol.7 No.1

오존과 염소에 의한 대장균 살균에 미치는 온도의 영향

방기인,조춘구 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

In the drinking water treatment, chlorine has been used the most popular for disinfection last 100 years. Chlorination has not only disinfection effect but also oxidation of matgnesium and iron in the water. However, chlorine also reacts with natural organic matter in water to produce disinfection by-products such as trihalomethane and haloacetic acid which are recognized as carcinogenic materials. Therefore, alternative disinfection method has been developed like ozonation and UV radiation. The ozonation is one of the most popular method to achieve the same goal as chlorination in the drinking water treatment. In the chlorination, various chlorine was used from 0.05 to 2.0mg/L with 30 to 60 seconds contact time. After 30 seconds cotact time with 1mg/L and of chlorine, 99% of Escherichia coli was killed while 0.5mg/L of chlorine was used with 60 seconds contact time. To meet the drinkin water regulation 1mg/L and 0.5mg/L of chlorine shold be used for 30 and 60 seconds, respectively. For ozonation, 0.05 to 1.56mg/L of ozone was used. For ozonation, 0.26mg/L of ozone was used with 20℃ while 1.04mg/L of ozone with 4℃ for 99.7% E. coli disinfection.

유기 과산화물이 Polypropylene의 유동 특성에 미치는 영향

윤홍식,최병오,조춘구 崇實大學校 1988 論文集 Vol.18 No.-

이노시톨과 피틴산이 피부에 미치는 영향

조춘구 ( Choon Koo Zhoh ),한창규 ( Chang Giu Han ),송환 ( Hwan Song ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.4

쌀눈에서 추출한 inositol과 phytic acid를 화장품에 적용하기 위해 피부에 미치는 영향에 대해 연구하였다 20~40대의 아시안 여성 45명을 대상으로 7주간 inositol 0~3.0 wt%와 phytic acid 0~1.50 wt%가 함유된 스킨로션을 제조, 사용 후 피부의 수분, 유분, 탄력, 주름에 미치는 영향에 대해서 알아보았다 Inositol 1.0 wt%가 함유된 스킨로션을 사용한 경우 피부의 수분 함유량은 19% 증가하였으며, 주름은 12.4% 개선되었고, 피부탄력은 12.4% 증가되었다. 또한 phytic acid 0.50 wt%를 함유한 스킨 로션을 사용한 경우 피부의 수분은 71.6% 증가하였으며, 주름은 16.9% 감소하였고, 탄력은 21.9%가 증가하였다. Inositol과 phytic acid를 지성과 건성피부에 적용한 결과 2~4주 후면 정상피부의 유분량으로 조절됨을 알 수 있었다 Inositol과 phytic acid는 모두 피부개선에 효과적이나, phytic acid는 민감한 피부에는 적용하기 어려운 점이 있다. 따라서 1.0 wt%의 inositol에 0~0.5 wt%의 phytic acid를 첨가한 스킨로션을 제조하여 그 효과에 대해서 알아보았다. 이 경우 1.0 wt%의 phytic acid에 1.0 wt%의 inositol을 사용한 경우 수분은 63.8%, 탄력은 17.2% 증가하였으며, 주름은 17.4% 개선되었고, 피부 유분량은 약 2주 후에 정상적인 상태로 개선됨을 알 수 있었다. 따라서 inositol 1.0 wt%에 0.10 wt%의 phytic acid를 첨가하여도 피부 개선효과가 나타남을 알 수 있다. 본 연구의 결과 내에서 1.0 wt%의 phytic acid와 1.0 wt%의 inositol을 사용한 경우 적은 양으로도 피부 개선 효과가 우수한 상승효과가 나타남을 알 수 있었다. Inositol and phytic acid extracted from rice bran were investigated for applying cosmetics. Skin lotions containing 0~3.0 wt% inositol and 0~1.50 wt% phytic acid were applied respectively, to the arm skins of 45 Asian women 20'~40's for 7 weeks. Improvement on moisture was evaluated. In addition, improvements on sebum, elasticity, and wrinkle were examined after applying placebo, inositol and phytic acid-containing skin lotions tot face, respectively. For 1.0 wt% inositol, it resulted in 19% increase of moisture. The wrinkle reduction and elasticity improved 12.4% and 17.0% on average, respec-tively. Applying 0.5 wt% phytic acid resulted in 71.6% increase the moisture. Improvements on wrinkle and elasticity were 16.9% and 21.9% respectivelv. hpplving inositol or phytic acid regardless of dry or oily skin, resulted in sebum value recovery to that of the normal skin after 2~4 weeks. Although inositol is inferior to phytic acid in improvements of the skin, phytic acid is not suitable to sensitive skin. So, 0~0.50 wt% of phytic acid were added to 1.0 wt% inositol and similar experiments were carried out. In case of added 1.0 wt% phytic acid, moisture increased 63.8% approximately. Improvements on elasticity and reduction on wrinkle were 17.2% and 17.4%, respectively. Both skin types were turned to normal skin type after 2 weeks. It could improve the skin condition when used inositol added phytic acid. The optimized concentration of phytic acid was 0.10 wt% with 1.0 wt% of inositol without side effect.