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Aureobasidium pullulans C-23 균주에 의한 Fructosyl Transferase의 생산 최적 배양조건
조원태,임재윤 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.4
선발균의 효소생산성 향상을 위해 배양과정의 특징을 조사하였다. 효소생산을 위한 최적의 탄소원은 sucrose(35%)이고, 질소원은 ammonium oxalate와 yeast extract를 함께 넣어 주었을 때이었다. 5l jar fermentor에서 선발균의 배양형태를 조사한 결과 2-3일 배양기간에는 pH가 4 이하로 현저하게 감소하는 것을 관찰하였고 4일 이후 다시 pH가 5-6 사이로 증가하였다. 균의 성장은 4일까지 증가하였다. 균체내 효소의 생성은 2일까지는 현저한 증가를 보이고 3-4일 정도 큰 변화를 나타내지 않으며 5일 이후 감소하였다. 균체외 효소는 5-6일까지 계속 증가하는 형태를 나타내었다. Glucose로 24시간 동안 미리 배양한 후 sucrose를 첨가하여 배양했을 때 균체내 효소의 생산이 현저히 증가하는 것을 알 수 있었다. For optimal production of fructosyl transferase in Aureobasidium pullulans C-23, the effect of fermentation conditions for cell growth and fructosyl transferase production were investigated. Sucrose was excellent carbon source. Sucrose concentration for the optimum production of fructosyl transferase was 35%. Enzyme productivity was significantly increased by addition of ammonium oxalate and yeast extract. A time course study for the enzyme production by Auteobasidium pullulans C-23 was carried out. At 2 days incubation, the production of intracellular enzyme was maximum. The extracellular enzyme production was found to be increased up to 6 days.
Effect of Janus Kinase 3 Inhibitor on Sebaceous Gland Regeneration during Skin Wound Healing
조원태,김아영,우현구,송해준,백은주 대한피부과학회 2023 Annals of Dermatology Vol.35 No.4
Background: Janus kinase (Jak) 3 has recently been shown as a beneficial target for the treatment of chronic inf lammatory diseases, such as psoriasis and alopecia areata. The role of Jak3 in tissue repair and remodeling is emerging. Objective: This study aimed to investigate the role of Jak3 signaling in the remodeling of the sebaceous gland (SG) during skin wound repair, and the development of in vitro SGs. Methods: Mouse skin tissue (ICR mouse) was obtained from the recovered skin eight days after a 4 mm biopsy punch wound. To observe the role of Jak3, the selective inhibitors WHI- p131 and PF06651600 was administered. Formation of in vitro SG was examined using pri- mary sebocyte cultures obtained postnatally from 3-day-old mice. Results: The data showed that SGs showed highly positive signals with anti-isolectin B4, which also used for detection of angiogenetic vessels and the basal epidermis. Isolectin B4 could be a good indicator of SGs. The Jak3 inhibitors significantly reduced the area and vol- ume of SG remodeling with reduced expression of p-Jak3. In addition, the area of cultured intact SG in vitro was significantly decreased in a concentration-dependent manner by Jak3 inhibition. Conclusion: These data showed that Jak3 signaling is a potent regulator to develop SGs. Jak3 inhibition did not decrease the number of sebocytes in SGs but decreased the area and volume of SG remodeling. Therefore, Jak3 inhibition may be a potential target for the treat- ment of SG hyperplasia and associated skin diseases.