Effect of Palm or Coconut Solid Lipid Nanoparticles (SLNs) on Growth of Lactobacillus plantarum in Milk

조연지,최미정,권윤중 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.2

This study was performed to investigate the effect of palm or coconut solid lipid nanoparticles (PO-SLNs or CO-SLNs) on growth of Lactobacillus plantarum (L. plantarum) in milk during storage period. The PO or CO (0.1% or 1.0%) was dispersed both in distilled water (DW) and ultra high temperature milk (UHTM), and subsequently emulsified with Tween® 80 by ultrasonication (30% power, 2 min). Increase in particle size and encapsulation efficiency (EE%) in DW was observed with an increase in oil concentration, whereas a decrease in ζ-potential of SLNs was noted with an increment in oil concentration. Moreover, the CO-SLNs exhibited relatively smaller particle size and higher EE% than PO-SLNs. The CO-SLNs were found to be more stable than PO-SLNs. Higher lipid oxidation of PO or CO-SLNs in UHTM was observed during the storage test, when compared to PO or CO-SLNs in DW. However, there was no remark- able difference in lipid oxidation during storage period (p>0.05). In the growth test, the viability of L. plantarum in control (without PO or CO-SLNs in DW) exhibited a dramatic decrease with increasing storage period. In addition, viability of L. plantarum of PO or CO- SLNs in UHTM was higher than that of SLNs in DW. Based on the present study, production of SLNs containing PO or CO in UHTM is proposed, which can be used in lactobacilli fortified beverages in food industry.

한국인 안허혈증후군환자의 초기 임상양상

조연지,최승권,박선호,이재정,이지은,박성후 대한안과학회 2019 대한안과학회지 Vol.60 No.12

Purpose: To evaluate the clinical features of ocular ischemic syndrome in Koreans. Methods: The medical records of patients who were presumed to have ocular ischemic syndrome by ophthalmic examination, and confirmed by carotid artery imaging, were retrospectively reviewed from 2010 to 2017. Results: A total of 22 patients and 27 eyes were included in the study. Twenty patients were male. The average age was 64.2 years. Fifteen patients had hypertension and fifteen patients had diabetes mellitus. Twenty-one patients presented with acute visual impairment at the initial visit. The average best-corrected visual acuity (BCVA) was a LogMAR of 0.89 ± 0.65, with an average IOP of 16.1 ± 6.9 mmHg. Elevated IOP > 21 mmHg was noted in five eyes (18.5%). Iris neovascularization was the most common (13 eyes, 48.1%) feature in the anterior segment. Retinal hemorrhage was the most common feature in the fundus examination (23 eyes, 85.2%). The average central submacular thickness was 255.0 μm, and there was no macular edema except for one case with vitreomacular traction syndrome on optical coherent tomography. Conclusions: A total of 90.9% of patients with ocular ischemic syndrome were males > 50 years of age. Most patients presented with acute visual impairment. There was no macular edema on optical coherent tomography of all eyes. 목적: 한국인 안허혈증후군환자의 초기 임상양상에 대해 알아보고자 한다. 대상과 방법: 2010년에서 2017년 사이 본원 안과에서 형광안저혈관조영을 포함한 안과검사에서 안허혈증후군으로 추정되고 영상학검사를 통해 경동맥 협착이 확인된 환자의 의무기록을 후향적으로 분석하였다. 결과: 22명 27안이 포함되었다. 20명은 남성이었고, 나이의 평균은 64.2세였다. 당뇨와 고혈압이 각각 15명에서 동반되었다. 21명에서 갑작스럽게 발생한 시력저하가 주 증상이었다. 최대교정시력은 logMAR 0.89 ± 0.65였고, 안압의 평균은 16.1 ± 6.9 mmHg였다. 21 mmHg 이상의 안압상승은 5안에서 확인되었다. 홍채신생혈관이 가장 흔한 전안부 소견이었고(13안, 48.1%), 망막출혈(23안, 85.2%)이 가장 흔한 안저검사 소견이었다. 빛간섭단층촬영을 통해 측정된 중심망막두께의 평균은 255.0 μm로 유리체황반견인이 동반된 1안을 제외하고는 황반부종은 없었다. 결론: 안허혈증후군환자의 90.9%는 50세 이상 남성이었고, 가장 흔한 임상 소견은 급격한 시력장애였다. 빛간섭단층촬영에서 황반부종이 나타나지 않는 것이 특징적이다.

야그레이저를 이용한 홍채낭종 절개술의 임상 효과

조연지,손유빈,이종수 대한안과학회 2022 대한안과학회지 Vol.63 No.3

목적: Neodymium-doped yttrium aluminium garnet (Nd:YAG) 레이저 단독으로 홍채낭종 절개술을 시행하였을 때의 치료 효과 및각막내피세포에 미치는 영향에 관해 알아보고자 한다. 대상과 방법: 세극등현미경 검사에서 홍채낭종으로 각막내피세포의 감소나 시축을 가려 시력 저하가 있던 3안을 대상으로 하였다. 초음파생체현미경 검사로 확진하였고, Nd:YAG 레이저 단독으로 홍채낭종 병변에 절개술을 시행하고, 첫 방문과 마지막 외래 내원시점에서 logMAR 시력, 안압과 각막내피층의 형태 변화를 관찰하였다. 결과: 평균 나이는 54.0 ± 5.3세이었고, 평균 경과 관찰 기간은 11.7 ± 2.1개월이었다. 치료 전 시력은 logMAR 0.23 ± 0.25, 치료후 시력은 logMAR 0.16 ± 0.15로 치료 후 시력이 호전되었으나, 통계적 유의성은 없었다(p=0.317). 평균 안압은 치료 전 14.7 ± 2.3 mmHg, 치료 후 13.7 ± 0.6 mmHg로 치료 전후에 통계적으로 유의한 차이는 없었다(p=0.276). 치료 전후의 각막내피세포 밀도, 육각형세포 비율, 세포면적 변이계수 변화량을 반대안과 비교하였을 때 역시 유의한 차이는 없었다(p=0.109, p>0.999, p=0.285). 시술후 심각한 합병증이나 홍채낭종이 재발한 경우는 없었다. 결론: Nd:YAG 레이저 단독 홍채낭중 절개술 후 각막내피세포의 밀도와 형태의 변화가 적어, 홍채낭종으로 각막내피세포의 감소가있거나, 시축을 침범하여 시력이 저하되는 경우의 효과적인 치료 방법으로 고려된다.

Changes in Quality Characteristics of Pork Patties Containing Multilayered Fish Oil Emulsion during Refrigerated Storage

조연지,권윤중,민상기,최미정 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.1

This study was performed to determine the effect of multilayered fish oil (FO) emulsion without or with trans-cinnamal-dehyde on pork patties. Multilayered FO (-primary, -secondary, -tertiary) emulsions were prepared using a layer-by-layer deposition technique with Tween 20, chitosan, and low methoxyl pectin, and were added to pork patties at the same concentration. Pork patties were then stored for 20 d in a refrigerator (5℃) to study changes in quality. The results showed that the pH value of all samples significantly decreased but cooking loss increased during storage (p<0.05). However, water-holding capacity and moisture content showed no remarkable difference between treatments and storage periods (p>0.05). All pork patties containing multilayered FO (treated samples) showed higher values for lightness and significantly lower values for yellowness compared to control pork patties (untreated sample). Lipid oxidation was higher in treated pork patties than in control pork patties during storage. In addition, lipid oxidation and total viable bacterial count in pork patties decreased as the number of coating layers increased. However, hardness, cohesiveness, and springiness of all samples showed no significant change during storage (p>0.05) as compared to fresh pork patties. Furthermore, these did not remarkable change with addition of trans-cinnamaldehyde in all pork patties. From our results, we suggest that FO emulsion did not affect the texture characteristics of fresh pork patties, indicating that it could be used to improve the quality of pork patties by contributing high-quality fat such as unsaturated fatty acids.

실리콘기름 충전 급성망막괴사 눈에서 Ganciclovir 주입 후 발생한 망막독성

조연지,최승권,박선호,이재정,이지은,박성후 대한안과학회 2020 대한안과학회지 Vol.61 No.1

Purpose: To report a case of retinal toxicity after an intravitreal ganciclovir injection to treat acute retinal necrosis in an eye filled with silicone oil. Case summary: A 56-year-old male presented with ocular pain and visual loss in his right eye. His best-corrected visual acuity was 20/25, inflammatory cells in the anterior chamber, multiple retinitis lesions and retinal vessel occlusions in the peripheral retina and vitreous opacity were showed. Acute retinal necrosis was suspected, anterior chamber polymerase chain reaction (PCR) test was done. Aciclovir 2,400 mg/day intravenously and ganciclovir 2.0 mg were administered by intravitreal injection. After 4 days, retinitis was worsened and PCR test was positive for varicella zoster virus. Ganciclovir intravitreal injections were increased twice a week. After 16 days, retinal detachment occurred, so scleral encircling, vitrectomy, laser photocoagulation, and silicone oil tamponade were conducted. Ganciclovir 1.0 mg was injected at the end of surgery. The patient’s visual acuity decreased to hand motion, and multiple crystal deposits with multiple retinal hemorrhages were observed in the right eye the next day. Visual acuity did not recover and optical coherent tomography showed that the macula was thinned. Conclusions: Visual loss seemed to be related with the retinal toxicity of ganciclovir. The increased local concentration due to the silicone oil tamponade is thought to have caused the toxicity. 목적: 급성망막괴사 환자에서 Ganciclovir 유리체강내 주입 후 발생한 망막독성 1예를 보고하고자 한다. 증례요약: 56세 남자가 우안 시력저하 및 통증으로 내원하였다. 최대교정시력 0.8, 전방 염증과 다수의 주변부 망막염, 혈관폐쇄, 유리체 혼탁이 관찰되었다. 급성망막괴사로 추정하여 전방수 중합효소 연쇄반응 검사와 aciclovir 2,400 mg/일 정맥주사, ganciclovir 2.0 mg 유리체강내 주입술을 시행하였다. 치료 4일째 병변 악화와 전방수에서 Varicella zoster virus가 확인되었다. Ganciclovir 유리체강내 주입을 주 2회로 증량하였다. 치료 16일째 우안 망막박리 소견으로 두르기공막돌융술, 유리체절제술, 눈속레이저, 실리콘기름 충전술과 ganciclovir 1.0 mg을 유리체강 내에 주입하였다. 수술 다음날 우안 최대교정시력 안전수동이었고, 황반부의 크리스탈 결정 형태침착물과 다발성 출혈이 관찰되었다. 이후 시력은 회복되지 않았고 황반의 얇아짐이 관찰되었다. 결론: 시력소실은 ganciclovir 망막독성에 의한 것으로 추정되며, 실리콘기름 충전으로 ganciclovir의 국소적 농도가 높아진 것이 원인으로 생각된다.

Characterization of β-Carotene Nanoemulsions Prepared by Microfluidization Technique

조연지,권윤중 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1

In the present work, β-carotene nanoemulsions,as potential active ingredients for liquid food, were preparedusing high pressure homogenization. The influence ofdifferent homogenizing conditions (pressure and number ofcycles) and emulsifier type and concentrations on particlesize parameters and content of β-carotene was investigated. The droplet size of the emulsions was found to decreasefrom 416.0 to 97.2 nm with increasing microfluidizationpressure, number of cycles, and emulsifier concentration. The optimum conditions for preparing β-carotene nanoemulsionswere determined to be homogenization pressureof 120 MPa and 3 cycles. The storage study showed thatthe nanoemulsions were physically stable for about 5weeks at room temperature (25oC). β-Carotene degradationwas considerably slower in WPI-stabilized nanoemulsionsthan in Tween 20-stabilized ones, which was attributed tothe increased surface area. These results have importantconsequences for the design and utilization of food-gradenanoemulsions.

Antioxidant activity of β-cyclodextrin inclusion complexes containing Trans-cinnamaldehyde by DPPH, ABTS and FRAP

조연지,조한슬,천지연 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.6

This study was carried out to observed b-cyclodextrin(b-CD) inclusion complexes containing transcinnamaldehyde(CIN) by using DPPH, ABTS and FRAPassay. Antioxidative ability was compared between pureCIN and b-CD-CIN inclusion complexes and particle size,encapsulation efficiency, and temperature-dependentrelease of inclusion complexes were investigated. Highconcentration of b-CD (1.8%) as well as guest oil 1:3 molarratio (b-CD:CIN) influenced on particle size bigger duringself-assembly process. And particle sizes were increased asstorage period. In the antioxidant capacity results, pure b-CD (1.8%) was antioxidative without CIN especially atFRAP assay. Antioxidant activity dramatically increasedafter 1:1 molar ratio (1.8% b-CD:CIN), especially at DPPHassay and ABTS•? assay. In this study, b-CD complexationenhanced CIN solubility and affected increase the antioxidantactivity of the CIN. Moreover, we need to considerthat molar ratio of between b-CD concentration and CIN iseffective manufacturing condition to improve antioxidantactivity of b-CD-CIN inclusion complexes.

Effect of NaCl Concentration on the Emulsifying Properties of Myofibrilla Protein in the Soybean Oil and Fish Oil Emulsion

조연지,권윤중,민상기,최미정 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.3

The aim of the present work was to investigate the effect of NaCl concentration on the emulsifying and rheological properties of porcine myofibrillar protein (MF)-stabilized soybean oil and fish oil emulsion (SO-EMs and FO-EMs). Emulsions (EMs) were prepared from 1% MF with 10% SO or FO at various NaCl concentration (0-0.5 M). The emulsifying ability index (EAI) of the EMs increased with increasing NaCl concentration for both oil types. Conversely, increasing NaCl manifested decrease in the emulsion stability index (ESI). In addition, creaming index (CI) also increased with NaCl concentration. From the microscopic observation, droplets of the EMs were more aggregated at relatively higher NaCl concentrations, especially for FO-EMs. All EMs had a gel-like structure owing to G' > G" from the rheological analysis. Comparing the oil types, the emulsifying capacity of SO-EMs was more stable than that of FO-EMs at all NaCl concentrations as determined from the CI value and microscopic observation. Therefore, it can be concluded that SO-EMs and FO-EMs are more stable at relatively lower concentrations of NaCl. In addition, the dispersed stability of SO-EMs was better than that of FO-EMs at the same concentration of NaCl.

통일대비 : 기획시리즈 ; 내가 본 남한사회 : 북한겨울 생각에 김장,쌀 많이 준비했다 낭패봐

조연지 평화문제연구소 2003 통일한국 Vol.230 No.-

대체식품의 적용을 위한 콩 식물성 단백질 소재 개발

조연지 한국콩연구회 2023 한국콩연구회소식 Vol.363 No.0