Streptanthus tortus 자엽의 배양세포에서 사부세포 발달동안 Sucrose 능동수송계의 유도 시기

조봉희,Cho, Bong-Heuy 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.2

Parenchyma cells of Streptanthus tortus suspension cultures possessed the different transport system for aldose-formed D-glucose and for ketose-formed D-fructose. $K_{m}$ value for D-glucose and D-fructose were 0.28mM and 15.02mM, respectively. $K_{m}$ value of D-mannose was 0.44 mM which is similar to the D-glucose transport system, but D-mannose was transported also through its own special uptake system. Parenchyma cells possessed the transport system of L-glucose, but the function of L-glucose was not known at all. Protoplast of parenchyma cells possessed only the monosugars transport system, but didn't possess the disugars, sucrose transport system. Early developing phloem protoplasts possessed glucose and sucrose transport system at the same time. On the contrary, in the complete developed phloem cells disappeared preexisted glucose transport system in the parenchyma cells, only new induced sucrose transport system existed.ted. 유소직 세포들은 aldose인 D-glucose ketose인 D-fructose에 대하여 다른 능동 수송계를 소유하고 있었다. D-glucose와 D-fructose 수송계의 $K_{m}$ 값은 각각 0.28 mM과 15.02mM이었다. D-mannose는 $K_{m}$ 값이 0.44 mM로 D-glucose와 유사하였지만, 그러나 D-glucose와는 다른 수송계를 소유하고 있었다. L-glucose도 고유한 수송계를 통하여 세포내로 수송되었으며, 그러나 그 기능을 전혀 알지 못하고 있다. 유조직 원형질체는 단당류 능동 수송계만을 소유하고, 이당류인 sucrose능동 수송계는 소유하지 않고 있었다. 발달 초기단계에 있는 사부 원형질체는 glucose와 sucrose 수송계를 동시에 소유하고 있었다. 완전히 발달된 사부세포에서는 이미 존재하였던 glucose 능동 수송계는 사라지고, 새로 유도된 sucrose 능동 수송계만 존재하는 것으로 측정되었다.

소년가장 가정의 실태와 대모제 서비스에 관한 연구

조봉희 한국복지재단 1990 동광 Vol.87 No.-

Ⅰ. Purpose The study is to provide effective and systematic drive of welfare service of the improvement in quality of child-headed family and to meet their needs and problems of the psychological and emotional development by accessing to homemaker service to center godmother system as well as analyzing their life status. Ⅱ. Mothod Sample method is based upon selecting of 232 households living in Daejon an Chungchungnam-do as random sample survey. Questionaires were made and sent to them to find out the status of their life and godmother system. Of the total 136 answers, 33 answers were from Daejon and 103 answers were from Chungchungnam-do. Ⅲ. Co-relation between Godmother and home maker services 표 Ⅵ. General Situations 1. The percentage of godmother system of child-headed family living in large city was 66.7% and that of middle city was 86.7 and that of rural region was 71.6. 2. The system of godmother functioned by neighbours was 15.3 percent, that of relatives 21.4 percentage and that of volunteers was 63.3 percent. 3. The percentage of child-headed family meeting with their godmother at least once a week was 53.3 percent. 4. the services of godmothers were communication, laundry, house cleaning, advice, family education, ettiquet, sex education, and financial support. 5. 78 percent of child-headed family were being satisfied with their godmother services. 6. They really want counselling with their godmothers for difficulties. 7. They want services from their godmothers until they as well as their siblings finish high school. 8. They hope the age of their godmother be 30-40's similar to their real mother. Ⅴ. Proposals on improvement for homemaker service. 1. Homemaker service should be formed like the following chart. 표 2. Welfare office should belong to local administrative office : city, county, ward 3. The services of welfare office should meet the needs of community residents, publice aid and charge the affair services of Child Welfare Law, Law for the Aged, Law for the Disabled Ruin Action Ban Act and Poor Female-headed Family Welfare Law 4. The public aid should support their needs for the culture as well as the suitest life of them. 5. To solve their emotional problems and to assist the special services of social welfare substantially should changed from godmother system to homemaker service.

배추과 식물현탁배양 세포내에서 산성 아미노산의 능동수송

조봉희 한국식물생명공학회 1995 식물생명공학회지 Vol.22 No.3

생리적인 pH에서 음전하를 띄우고 있는 산성 아미노산인 aspartate와 glutamate는 다른 중성 아미노산과 경쟁방해 실험을 행한 연구결과에 의하면 이들도 중성 아미노산이 갖는 능동운반계와 동일한 운반자를 소유한다. 중성 아미노산을 아미노산 한 분자 당 한개의 수소이온과 동반수송되고 전하에 보상을 위해서 한 분자 당 한개의 칼륨 이온을 배출한다. 그러나 산성 아미노산은 한 분자 당 2개의 수소이온과 동반수송되고, 한 분자 당 칼륨 한 분자를 배출한다. 중성 아미노산과 같은 운반계를 소유하고, 2개의 수소이온과 동반수송되는 능동운반계는 본 실험에서 처음으로 보고된다. 이 결과로부터 두개의 동반수송된 수소이온 중 한 개는 산성 아미노산에 있는 pK$_3$카르복실기를 먼저 중화시킨 후에 중성화된 형태로 수소이온 한 분자와 동반수송된다고 사료된다. 그러므로 유채는 20개의 아미노산에 대해서 다만 2개의 운반계, 즉 일반- 과 알카리성 아미노산 운반계를 가진 것으로 확인되었다. 다른 식물에서 이미 보고된 결과들을 참고하여 아미노산 운반계의 진화적인 의미를 고찰하였다. The acidic amino acids, aspartate and glutamate, which have a negative charge in physiological pH, possess the same transport system as neutral amino acids according to the competitive inhibitory studies with the neutral amino acids. The neutral amino acids cotransported with one H+ per molecule, and one K+efflux per one molecule for charge compensation (Cho,1994), but the acidic amino acids cotransported with two H+ per one molecule, and one K+ efflux per one molecule. The active transport system, which possess the same carrier but cotransported with the different number of H+, reported for the first time. from the results, we can see that one of cotransported H+ protonated at first carboxyl group of pK$_3$ of acidic amino acids, and then as a neutral form cotransported with H+ Therefore, Brassica possess two amino acids transport system for 20 amino acids, namely general - and basic amino acids transport system. The evolutionary meaning of amino acid carriers described with other reported plants.

Streptanthus tortus의 培養細胞로부터 사부 세포의 분리와 분리된 篩部 및 柔組織 細胞에서 설탕의 능동수송

조봉희 한국식물생명공학회 1998 식물생명공학회지 Vol. No.

Streptanthus 조직 배양 세포를 사용하여 사부를 순수분리 시키고, 분리된 사부에서 사부 하적에 대한 기작을 규명하기 위해 다음 연구를 하였다. 유조직 세포는 0.2% macerase 와 0.03% cellulase의 가수분해 효소로 처리하여 원형질체를 얻었고, 분화된 세포에서는 0.03% cellulase + 0.02% pectinase + 0.2% macerase + 0.025% rohamet PC로 가수분해시켜서 순수한 사부 세포와 사부 원형질체와 반세포의 원형질체를 분리하였다. 분리된 유조직 세포와 반세포의 원형질체에서는 단당류인 포도당을 수송시키나, 설탕은 수송시키지 못했다. 반면 분리된 사부 세포는 설탕을 능동수송 시키나 포도당은 수송시키지 못했다. 이는 설탕의 사부 하적은 반세포 없이도 가능하며, 반세포는 설탕 운반체가 없어서 설탕을 직접 수송할 수 있는 능력이 없다는 것을 보여주는 것이다. 그리고 사부 세포에서 설탕의 수송은 에너지대사에 의존하는 능동수송으로 나타났다. Protoplasts were isolated from the parenchyma supension cultured cells of Streptanthus tortus using hydrolytic enzymes, 0.03% cellulase + 0.02% pectinase. Phloem cells and companion protoplasts were isolated from differentiated suspension cultured cells using hydrolytic enzymes, 0.2% macerase + 0.03% cellulase + 0.02% pectinase + 0.025% rohamet PC. Isolated parenchyma -and companion- protoplasts transported glucose into the cells, but not transported sucrose at all. On the other hand, isolated phloem cells transported sucrose into the cells actively, but not transported glucose. These results show for the first time that loading of sucrose into the phloem cells without nucleus was possible without contributing of companion cells and companion cells had not the ability to transport sucrose directly because of lack of sucrose carriers in the membrane. The sucrose transport into the isolated phloem cells depend on metabolic energy.

시설 결연사업에 관한 조사연구 -충청남도 육아시설 아동을 중심으로-

조봉희 초록우산 어린이재단 1987 사회복지관 사례연구 Vol.3 No.-

식물성장 조절물질을 분비하는 미생물의 탐색

조봉희,김근,성낙문 ( Bong Heuy Cho,Keun Kim,Nack Moon Sung ) 한국균학회 1993 韓國菌學會誌 Vol.21 No.2

Chlorella에 물질 운반계에 대해서

曺鳳姬 水原大學校 1988 論文集 Vol.6 No.-

Chlorella vulgaris possess inducible sugar transport system. Through the inducible hexose transport system is transported 1-deoxyglucose, 3-0-methylgucose, 6-deoxyglucose, and other glucose analogues. Neutral sugars are cotransported with proton. Netto positive charged sugars are unitransported without accompanying with protons. The stoichiometry of sugar/ proton is about 1:1 Chlorella possess 7 different amino acid transport systems naturelly. Among of them, two amino acid transport systems are induced by glucose, one is arginine transport system, the other is neutral amino acid transport system. About the two inducible amino acid transport system are discussed.

상보형 전기변색소자용 V2O5 박막의 대향전극 특성

조봉희,김영호 ( Bong Hee Cho,Young Ho Kim ) 한국전기전자재료학회 1996 전기전자재료학회논문지 Vol.9 No.7

피마자 자엽에서 ABA 처리에 의한 Polyamine 농도의 영향

조봉희 수원대학교 기능성생명소재연구소 2005 자연과학연구논문집 Vol.4 No.1

The concentration of polyamine(PA) and the relationship between the concentration of PA and ABA resistant were analysed during the ABA treatment in the cotyledons of Ricinus communis.. The content of putrescine(PUT), spermidine(SPD), and spermine(SPM) was changed significantly all the stress time. PA was synthesized against ABA or separated from bound PA during the ABA stress. The content of SPD and SPM was increased, but PUT was decreased dramatically during the continuously ABA stress. we suggest that SPD and SPM were responsible for the increase of the resistance of water stress against the ABA signal.

설탕의 수송동안 Ca^2+ 이온과 고농도의 설탕에 의한 비율의 변화

조봉희 수원대학교 기초과학연구소 1993 基礎科學論文集 Vol.2 No.-

The stoichiometries of H^+/sucrose 0.28 and K^+sucrose 0.38 were not changed significantly with low concentration of sucrose. The addition of high concentration of sucrose to cotyledons changed the stoichiometries of K^+/H^+, one proton taken up per approx, two potassium ions released. In the presence of CaCl_2, the stoichiometry was changed up to 0.54 protons taken up per sucrose and up to 0.6 potassium ions released per sucrose.