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Alcaligenes latus 의 배양방법에 따른 생분해성 플라스틱의 생산특성
조경숙,최희식,류희욱,조강현,박성연 ( Kyung Suk Cho,Hee Sik Choi,Hee Wook Ryu,Kang Hyun Cho,Seong Yeon Park ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1
Optimum conditions for cell growth and poly (3-hydroxybutyrate, PHB) biosynthesis of Alcaligenes latus. which can accumulate PHB simultaneously in associated with cell growth and grows well on a cheap carbon source such as sucrose, were investigated. The effect of initial sucrose concentration, ranged from 10 to 50g/L, on the cell growth and PHB accumulation were studied by a batch culture in a 5 L fermentor. PHB productivity was increased from 0.11 to 0. 36 g-PHB/L·h with increasing sucrose concentration up to 30g/L, and it was fairly constant between concentrations of 30g/L and 50g/L. The maximum specific growth rate, 0.44h^-1, was obtained at 30 g-sucrose/L, and the growth was inhibited at concentrations of sucrose over 30g/L. A. latus was also cultivated to attain high PHB productivity by fed-batch or semicontinuous fed-batch culture techniques. PHB productivity by a fed-batch culture under phosphate limitation (1.14g-PHB/L·h) were smaller than that without nutrient limitation (2.10g-PHB/L·h), but there is no significant difference of PHB content (55-60%) obtained in both culture. By a semicontinuous fed-batch culture without nutrient limitation, high PHB productivity could be obtained as 4.5∼4.8 g-PHB/L·h. The average PHB content, the yield of dry cell weight, and the yield of per consumed sucrose were 60%, 0.48, and 0.32, respectively. These results suggest that the semi continuous fed-batch culture technique is one of promising methods to achieve high PHB productivity by A. latus.
부식된 하수관에서 분리한 곰팡이 OMSOf1 의 황화수소 제거 특성
조경숙,박상진 ( Kyung Suk Cho,Sang Jin Park ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2
A sulfide-oxidizing fungus was isolated from corroded concrete sewer pipes. From the physiological and morphological properties such as growth characteristics on diverse media, reproduction pattern, and size and shape of spores, the isolated fungus was considered as Sporormia sp. The fungus OMSOf1 could oxidize hydrogen sulfide (H_2S) to thiosulfate due to intracellular enzyme systems. The fungus was immobilized in polyurethane carriers which contained super absorbent polymer (sodium polyacrylic acid) permeated with mineral medium, and polyurethane. When 28ppm of H_2S was supplied to the biofilter packed with the carriers at 50㎖/min, more than 95% removal ratio of H_2S was obtained for 15 days without supply of water. The reduction of H_2S removal ratio by drying of the carriers could be recovered by supply of water.
조경숙,류희욱,Cho, Kyung-Suk,Ryu, Hee-Wook 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4
메탄은 온실효과가 이산화탄소 보다 20배 이상인 대표적인 non-$CO_2$ 온실가스이다. 매립지는 주요 인위적 메탄 발생원으로, 매립지의 메탄 발생량은 연간 35~73 Tg(tera gram)으로 추정된다. 바이오커버(개방형 시스템)과 바이오필터(폐쇄형 시스템)을 이용하는 생물학적 방법은 메탄을 회수하여 자원화하기에는 메탄 농도가 너무 낮거나 가스 포집정이 설치되어 있지 않는 노후화된 매립지나 소규모 매립지로부터 메탄 배출을 저감할 수 있는 유용한 방법이다. 메탄을 유일탄소원과 에너지원으로 활용하는 메탄산화세균은 이러한 생물학적 방법에 있어 메탄을 산화시켜 제거하는데 매우 중요한 역할을 담당한다. 토양, compost, 지렁이 분변토 등과 같은 다양한 충전재를 이용하여 실험실 규모의 바이오커버/바이오필터의 메탄산화효율에 관한 많은 연구가 진행되었다. 이 중에서 compost는 가장 많이 이용되고 있는 충전재이고, compost를 이용한 바이오커버/바이오필터의 메탄산화속도는 50에서 $700\;g-CH_4\;m^{-2}\;d^{-1}$로 보고되고 있다. 또한, 실제 매립지에 파일럿 규모의 바이오커버/바이오필터를 설치하여 메탄 배출 저감 효과에 관한 연구도 진행되고 있다. 매립지의 메탄 배출 저감은 탄소배출권 거래와 연관될 수 있으므로, 바이오커버/바이오필터에 의한 메탄 저감량을 정확하게 평가하는 것이 매우 중요하다. 그러므로, 매립지 현장에 설치된 바이오커버/바이오필터의 성능을 평가하는 방법은 표준화되어야 하며, 메탄 저감량을 정확하게 정량화할 수 있는 방법 개발이 필요하다. Methane, as a greenhouse gas, is some 21~25 times more detrimental to the environmental than carbon dioxide. Landfills generally constitute the most important anthropogenic source, and methane emission from landfill was estimated as 35~73 Tg per year. Biological approaches using biocover (open system) and biofilter (closed system) can be a promising solution for older and/or smaller landfills where the methane production is too low for energy recovery or flaring and installation of a gas extraction system is inefficient. Methanotrophic bacteria, utilizing methane as a sole carbon and energy source, are responsible for the aerobic degradation (oxidation) of methane in the biological systems. Many bench-scale studies have demonstrated a high oxidation capacity in diverse filter bed materials such as soil, compost, earthworm cast and etc. Compost had been most often employed in the biological systems, and the methane oxidation rates in compost biocovers/boifilters ranged from 50 to $700\;g-CH_4\;m^{-2}\;d^{-1}$. Some preliminary field trials have showed the suitability of biocovers/biofilters for practical application and their satisfactory performance in mitigation methane emissions. Since the reduction of landfill methane emissions has been linked to carbon credits and trading schemes, the verified quantification of mitigated emissions through biocovers/biofilters is very important. Therefore, the assessment of in situ biocovers/biofilters performance should be standardized, and the reliable quantification methods of methane reduction is necessary.
친환경 황토 보드를 이용한 경량 칸막이 벽체의 세대간 경계벽 적용을 위한 내화 및 차음 성능 연구
조경숙(Cho, Kyung-Suk),여인환(Yeo, In-Hwan) 대한건축학회 2013 大韓建築學會論文集 : 構造系 Vol.29 No.12
This study tested, in Long-Life Housing, partition wall between housing units which composite with middle lintel and Loess board, whether corresponded with requirements for performance criteria related with codes. It was get a performance level of Loess board that compared with gypsum board which used to partition wall components generally. It was tested requirements for fire resistance and sound insulation performance of partition wall in Korea Building Code. The results are, a insulation of the wall with gypsum board was better than Loess board, integrity of wall with loess board was better than gypsum board in fire. The sound insulation was, Loess board was better gypsum board due to porosity of loess and rice straw. Therefore, the partition wall between building units which composite with gypsum board on inside layer and Loess board on outside layer, with glass wool for insulation, is prefer to apply.
조경숙(Kyung-Suk Cho),류희욱(Hee Wook Ryu) 한국생물공학회 2015 KSBB Journal Vol.30 No.5
In recent years, there has been an intensive research on the application of degradative activities of fungi for treatment of various non-degradable materials such as petroleum hydrocarbons, polychlorinated biphenyls, pesticides, polycyclic aromatic hydrocarbons, dyes and so on. Chief of all, the fungal treatment technology is received the spotlight as one of the most promising alternatives to replace present methods for the treatment of dye wastewater. The present paper reviews the recent trend in research on the decolorization and biodegradation of dyes by various fungi, and improvements in bioreactors and bioprocesses involved the fungal treatment of dye wastewater. It also discusses alternatives and perspectives for the innovation of mycoremediation to treat dye wastewaters.