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급성림프구성백혈병에서 PCR 을 이용한 잔존종양세포의 검출
조경삼(Kyung Sam Cho),김정희(Jeong Hee Kim),김영일(Young Il Kim),김선희(Sun Hee Kim),김시영(Si Young Kim),윤휘중(Hwi Joong Yoon) 대한내과학회 1996 대한내과학회지 Vol.50 No.4
N/A Objectives: Immunoglobulin heavy chain(IgH) gene rearrangements are specific for each lymphoblastic leukemic clones. We studied sensitivity and specificity of clonospecific probe produced by amplification of IgH gene by polymerase chain reaction (PCR). Methods : 7 acute lymphoblastic leuketnia(ALL) patients who had Igk3 gene rearrangement by southern blot analysis were studied. DNA of patients at diagnosis were extracted from bone marrow mononuclear cells and amplified by PCR with primers recognizing consensus sequence of V(variable) and J(joining) region of IgH gene, Clonospecific probe produced directly from PCR products without sequencing. Results: In 6 patients, rearranged band was detected on electrophoresis after PCR. Dot blot of the amplified products were hybridized with each clonspecific probe produced by simplified method using PCR. 6 probes from each patient detected PCR products from its own patient DNA, while no cross-hybridization was found in the 6 samples. The sensitivity of this method was 10-3. Conclusion: The clonospecific probes were produced by simplified method without sequencing. The probes produced showed excellent clono-specificity but low sensitivity. Further studies required to improve sensitivity of clonspecific probe and to evaluate clinical significance of minimal residual disease(MRD).
조경삼(Kyung Sam Cho),김덕윤(Deog Yoon Kim),양형인(Hyung In Yang),김의종(Eui Jong Kim),류경남(Kyung Nam Ryu) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.2
N/A We studied 90 patients(179 femoral heads) with avascular necrosis of femoral head, who had been performed X-ray, bone scan and MRI to copare of the findings of AVN on bone scan between each other, retrospectively. The patients were 82 males and 9 females, their mean age was 45 years. Radiographic stages were classifted by Steinberg modification, radionuclide stages were classified as followed; stage o(or type 0): noraml, stage 1: faint ring like uptake around the femoral head, stage 2: intense ring like uptake, stage 3: irregular increased uptake with central photon defect, stage 4: intense diffuse increased uptake at fem- oral head and stage 5: hip joint deformity with relatively mild increased uptake. The findings of MRI were classified according to extent, location, early or advanced lesion, signal int,ensity of the lesion and joint effusion. 156(87%) of 179 femoral heads had avascular necrosis, 68(75.5%) of 90 patients had bilateral AVN, 36 femoral heads had early stage and 120 had advanced stage. The detection rate of AVN by X-ray and bone scan were 85%(134), 91.6%(143), respectively. Early AVN with atypical types of bone scan showed larger extent, moderate to large amount of joint effusion, soft tissue hypertrophy within joint, and secondary degenerative changes. Bone scan had relatively high detection rate in the diagnosis of AVN of femoral head, and demonstrated various t.ypes depending on the disease stage.
김선희(Sun Hee Kim),김시영(Si Young Kim),윤휘중(Hwi Joung Yoon),조경삼(Kyung Sam Cho),김상인(Sang In Kim) 대한내과학회 1991 대한내과학회지 Vol.41 No.4
N/A Immunophenotypic studies have a well-documented significance in the determination of cellular lineage in lymphoproliferative disorders. With the exception of mature B-cell disorders, it is difficult to demonstrate cellular clonality by immunophenotypic studies. The advent of specific DNA probes for immunoglobulin and T-cell receptor genes has greatly facilitated the detection of clonality. Although the scientific basis of the study of gene rearrangements has been well established, routine diagnostic roles and clinical significances are uncertain. Using southern hybridization, this analysis has aimed at evaluating the status of each of the known Ig and TcR loci in various lymphoproliferative disorders, exploring the relationship between lineage assessment by immunophenotyping and genatyping and the clinical significance of Ig and TcR genotypic heterogeneity. Analysis of gene rearrangements show considerable heterogeneity even in the same morphologic and immunophenotypic groups. Although gene rearrangement study was superior to immunophenotyping in detecting clonality, especially in which standard techniques had failed to define a clonal cell population, immunophenotyping was more sensitive and informative for lineage assignment due to the high frequency of crosslineage rearrangements of the TcR and lg genes. No significant association was found between Ig and TcR genotype and clinical parameters. When appropriately applied, DNA rearrangement analysis complements conventional histology and immunophenotyping for diagnosis of lymphopraliferative disorders.
악성 성상세포종에서 표피성장인자 수용체 과발현의 임상적 의의
홍성언(Sepng Eon Hong),강진오(Jin Oh Kang),이혜경(Hye Kyung Lee),양문호(Moon Ho Yang),임 언(Won Leem),조경삼(Kyung Sam Cho) 대한방사선종양학회 1996 Radiation Oncology Journal Vol.14 No.2
Purpose: To determine the incidence and prognostic effects of EGFR over-expression in the high-grade astrocytomas. Materials and Methods: With 23 paraffin blocks of the high-grade astrocytomas (7 anaplastic astrocytomas and 16 glioblastoma multiforme), expression of EGFR were evaluated by immunohistochemical staining employing polyclonal antibody raised to short cytoplasmic domain of the molecule. Result : Two out of 7 anaplastic astrocytomas and 9 out of 16 glioblastoma multiforme patients showed overexpression of EGFR(p=0.44). Three out of 11 patients of age below 55 and 8 out of 12 patients of age over 54 showed EGFR overexpression(p=0.141). Median survival of the EGFR negative anaplastic astrocytoma patient was 37 months. Median survival of the glioblastoma multiforme patients were 11 months in EGFR negative group and 7 months in EGFR positive group. But survival difference was not significant (p=0.17). Conclusion: There was a marked trend of increasing overexpression of EGFR in older patients. But survival of the glioblastoma multiforme decreased by the overexpression of the EGFR without significant. 목적: 악성 성상세포종에서 표피 성장 인자 수용체의 과발현의 빈도와 예후 인자로서 가능성을 조사하기 위하여 본 연구를 시행하였다. 대상 및 방법: 조직학적으로 악성 성상세포종으로 확진되고 방사선 치료를 받은 23명(역형성성상세포종 7예, 다형성 교아세포종 16예)의 파라핀 블록에 antihuman EGFR polyclonal antibody를 이용하여 면역염색을 시행하였다. 결과: 표피 성장 인자 수용체는 역형성 성상세포종에서는 7예중 2예에서 양성이었고 다형성 교아세포종은 16예중 9예에서 양성으로 양군간의 발현 빈도의 차이는 통계적으로 유의하지 않았다 (p=0.44). 55세 미만의 환자는 11예중 3예에서 양성이었고 55세 이상은 12예중 8예에서 양성이었다(p=0.141). 표피 성장 인자 수용체 음성인 역형성 성상세포종 환자의 평균 생존기간(중앙값)은 37개월이었다. 다형성 교아세포종 환자의 평균 생존 기간은 표피 성장 인자 수용체 음성 군은 중앙값 11개월, 양성 군은 중앙값 7개월이었으나 두 군간의 통계적인 차이는 없었다(p=0.17). 결론: 55세 이상 연령군에서 표피성장인자 수용체의 과발현의 빈도가 높았다. 다형성 교아세포종 환자의 생존율은 표피성장인자 수용체 과발현에 의하여 감소하였으나 유의한 영향을 받지 않았다.