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Protective Effects of Wuyaoshunqisan Against H_2O_2-Induced Apoptosis on Hippocampal Cell Line HiB5
Je, Jeong-Jin,Shin, Hyun-Taeg,Chung, Seok-Hee,Lee, Jong-Soo,Kim, Sung-Soo,Shin, Hyun-Dae,Jang, Mi-Hyeon,Kim, Youn-Jung,Chung, Joo-Ho,Kim, Ee-Hwa,Kim, Chang-Ju WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-
Abstat: Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders . To investigate the protective effects of Wuyaoshunquisan against H^2O^2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H^2O^2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqyisan prior to H^2O^2 exposure.
윤성중(Sung Joong Yun),제정진(Jeong Jin Je),이훈(Hoon Lee),김유진(Yoo Jin Kim),이현삼(Hyun Sam Lee) 대한스포츠한의학회 2015 대한스포츠한의학회지 Vol.15 No.1
Objectives This study is attempted to evaluate the association between herbal medicine and the prohibited substances issued by the inves-tigation of anti-doping and propose the rational criteria for the selection of prohibited herbal medcine. Methods and Results The validity of 10 herbal medicines listed on anti-dopping which have been suspected of the possibility for the inclusion of the banned elements were reviewed. Of these, 4(Moschus berezovskii Flerove, Liriope platyphylla, Rehmanniae Radix Crudus, and Cistanche deserticola), 2(Poncirus trifoliata Rafinesque and Citrus aurantium L.), 1(Chinemys reevesii Gray), 1(Hominis Placenta), 1(Chelidonium majus L.), and 1(Pinellia ternata Breitenbach) were suspected of the inclusion of glycerol, synephrine, steroid, cor-tisone, codeine, and ephedrine, respectively. We found that 22 herbal medicines were not listed on the Korean Phamacopeia of Crude Drug and the distribution of 17 ones were forbidden, and compared the criteria for the selection of prohibited herbal medi-cine in Korea with these in China, Japan, and Taiwan. Conclusion We concluded that this study is likely to be the basis on the establishment of the accurate term for herbal medicines related to doping and the safety for ones by the scientific criteria.