一部紡織工場 女職工의 月經에 關한 調査硏究

鄭熺坤 최신의학사 1975 最新醫學 Vol.18 No.4

A survey on the menstruation of employee in textile plant was carried out on 1, 247 female in Kwang-ju city during the period from September 20 to September 301'973. The summarized results are asfollows: 1. An average age of sexual interest began was observed 16.5. The earlist age of sexual interest began was 9 (0.3 and the latest age 22 (0.1 % ) 2. An average age of menarche was observed 15.3. The earliest age of menarche was 10(0.2%) and the latest age 19 (0.9%). And then, season of menarche was observed in order of Autumn (32.9%), 3 Winter (25.3%), Summer (22. 1 %), and Spring(17.3%). 3. Menstrual cycle was observed regular in 30. 1%,and irregular in 62.9%; As to the average menstrual cycle was 28.4 days among the employee of regular menstrual cycle. 4. Types of menstrual cycle were varied in order of 30 days(28.5%), 28 days(26.6%), 25 days(15-4%), 35-days (8.8%), and 20 days (5.1%). 5. Length of period with blood flow was observed average 4.5 days in the before employment, and 4.3 .days inthe after employment. 6. Comparing the degree of amount of blood flow before employment, the answer was "less than before" in 41.5%, "same as.before" in 30.6%, and "more than before" in 8.6%. 7. Comparing the degree of subjective symptoms complaints during- the menstrual period before employment, the answer were "severer than before" in 49.7%, "same as before" in 38.8%, and "milder than before" in 5.4%. 8. Four main subjective symptoms complaints during the menstrual period were occupied in order of lumbago (38.3%), depression (35.2%)abnormal fatigue (33.6 % ), and abdominal pain (32.2%). 9.Working efficiency during the menstrual period was occupied slight diminution in 47.8%; "same as normal", in--26.9%;and "severe diminution" in 7%.

독소원성 대장균과 Clostridium perfringens A형이 생산하는 장독소의 검색을 위한 RPLA법과 PCR기법의 감도 비교 ( Comparison of Sensitivity of Detection for Enterotoxigenic Escherichia coli Enterotoxin and Clostridium perfringens Type A Enterotoxin by Means of the Reversed Passive Latex Agglutination and the Polymerase Chain Reaction )

정희곤 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.1

독소원성 대장균(enterotoxigenic Escherichia coli, ETEC, EC81, serotype O148:H28)이 생산하는 heat-labile enterotoxin(LT)를 검색해 본 결과, reversed passive latex agglutination(RPLA)법에 있어서는 2배로 회석한 용액(50 ng)으로부터 64배로 회석한 용액(1.56 ng)에서까지 양성반응을 보였으며 polymerase chain reaction (PCR)기법에 있어서는 10 ng으로부터 1 pg회석용액에서까지 417-base pair(bp)의 LT DNA fragment가 확인되었다. Clostridium perfringens A형(NCTC8238, Hobbs serotype 2)이 생산하는 장독소를 검색해 본 결과, RPLA법에 있어서는 2배로 회석한 용액(50 ng)으로부터 64배로 회석한 용액(1.56 ng)에서 까지 양성반응을 보여 독소원성대장균이 생산하는 LT와 일치하였으나, PCR기법에 있어서는 10 ng로부터 10 pg 회석용액에서 까지 364-bp의 DNA fragment가 확인되어 독소원성대장균이 생산하는 LT보다 1/10의 낮은 감도를 보였다. PCR기법은 RPLA법에 비하여 훨씬 신속하고 소량의 sample로 장독소를 확인할 수 있었다.

건강한 산양 및 개에서 분리한 Clostridium perfringens의 항생제에 대한 감수성

정희곤 한국식품영양학회 1998 한국식품영양학회지 Vol.11 No.3

1995년 12월부터 1996년 11월까지 약 1년간 광주시 및 전남지역에서 사육하고 있는 건강한 산양 20두와 건강한 개 30두, 총 50두로부터 분변을 채취하여 5균주의 C. perfringens을 분리 동정하였다. C. perfringens의 분리율은 건강한 산양 20두 중에서 3균주(15.0%)가 분리되었으며 건강한 개 30두 중에서 2균주(6.7%)가 분리되었다. C. perfringens의 항생제에 대한 감수성은 ampicillin, baytril 및 penicillin에 대해서는 분리균의 80%가 감수성을 나타내었고 cephalothin에 대해서는 60%가 감수성을 나타내었으며 erythromycin에 대해서는 40%가 감수성을 나타내었다.

요로감염소아의 오줌에서 분리한 대장균 K1 다당류 항원의 동정

정희곤 한국식품영양학회 1998 한국식품영양학회지 Vol.11 No.4

Identification of escherchia coli K1 polysaccharide antigen isolated from urine specimens of urinary tract infections in children were performed from of 1992 to 1993 in Kyoto, Japan. The serotypes of E. coli were categorized that O1:H7, O2:H6, O2:H7, O16:H6, O18:H7, O18:H￣, and O135:H44 among 14 strains isolated from urine specimens of urinary tract infections in children by the serological test. And, one strain (O18:H￣, isolation rate: 7.1%) of E. coli K1 polysaccharide antigen among 14 strains were isolated from urine specimens of urinary tract infections in children by the bacteriophage test.

축산 유래 식중독 세균에 관한 생태학적 연구 제2보 일부 도시 근교지역 젖소 유방염의 발생양상과 항생제 감수성의 경시 변화

정희곤 한국식품영양학회 1999 한국식품영양학회지 Vol.12 No.1

Bovine mastitis was examined by use of the rolling ball viscosity test in Kwang-ju area. Antibiotic sus-ceptibility of isolated pathogens from raw milk was investigated by the disc method. Positive rate of so-matic cell counts (over 500,000 cells/ml) was decreased from 11.1% in 1991∼1993 to 8.0-% in 1995∼1997. Isolated mastitis-causing-pathogens were identified as Staphylococcus sp. (49.7%) Escherichia coki(23.2%) Streptococcus sp. (10.4%) and Bacillus sp(7.2%) Antibiotic susceptibility of isolated pathogens to almost antibiotics was decreased.

일부 산업체 근로자의 성인병 발생양상

정희곤 한국식품영양학회 1992 한국식품영양학회지 Vol.5 No.2

Patterns in the outbreaks of senile diseases were investigated on 1, 264 workers by special examination among the 43, 210 industrial workers by the screening test performed in Kwang-ju and Chon-nam Branch of Korean Industrial Health Association from January 4 to December 31 in 1991. The results are summarized as follows : 1) Prevalence rate of senile diseases of industrial workers was 2.9%. And prevalence rate according to the kinds of senile diseases was observed in the order of hepatitis (0.9%), hypertension(0.7%), diabetes mellitus(0.5%), anemia(0.4%). 2) Prevalence rate of complicated senile diseases of industrial workers was 0.5%. And prevalence rate according to kinds of complicated senile diseases was observed in the order of others hypertension and arteriosclerosis (0.1%), HBV and others chronic hepatitis(0.1%), essential hypertension and arteriosclerosis(0.03%), iron deficiency anemia and leukopenia(0.03%).

독소원성 대장균(EC81)이 생산하는 이열성장독소와 Clostridium perfringens A형 (NCTC8238)이 생산하는 장독소의 검색을 위한 중합효소 연쇄반응기법의 감도 비교

정희곤 한국식품영양학회 2000 한국식품영양학회지 Vol.13 No.1

Detection for heat-labile enterotoxin(LT) of enterotoxigenic Escherichia coli(ETEC, EC81, O148:H28) and enterotoxin of enterotoxigentic Clostridium perfringents type A(CP, NCTC8238, Hobbs serotype 2) by use of a polymerase chain reaction (PCR) assay were positive reaction, which using LT gene-specific primers of ETEC with a detection limit equivalent from 100ng/${\mu}\ell$ to 1 pg of a DNA fragment of 417-bp in EC81 and enterotoxin gene-specific primers of CP with a detection limit equivalent from 100ng/${\mu}\ell$ to 10pg of a DNA fragment of 364-bp in NCTC8238. Detection for a LT gene of ETEC highly appeared 10-fold sensitivity than an enterotoxin gene of CP.

한국환경위생학회 추계학술논문발표연제 : 일부 동물에서 분리한 Clostridium perfringens 독소형의 검색

정희곤 대한보건협회 1998 대한보건협회 보건종합학술대회 Vol.1994 No.-

일부 동물에서 분리한 Clostridium perfringens 독소형의 검색

정희곤 한국환경보건학회 1998 한국환경보건학회지 Vol.24 No.3

Outbreak patterns were observed in 250 chickens of necrotic enteriris(0.3%) among 81,000, 90 enterotoxemic pigs(9.5%) among 950, and 9 enterotoxemic cattle(13.8%) among 65 in a farms of Gyeonggi, Chungnam, Kwangwon, and Chonnam area from 1993 to 1996. Clostridium perfringens was isolated from 36 strains(14.4%) among 250 chickens of necrotic enteritis, 21 strains(23.3%) among 90 enterotoxemic pigs, and 3 strains(33.3%) among 9 enterotoxemic cattle. Toxin types by the neutralization test were categorized as A-type in 36 strains(100.0%) among 36 chickens of necrotic enteritis, and as A-type in 17 strains(81.0%), as B-type in 2 strains(9.5%) and as C-type in 2 strains(9. 5%) among 21 enterotoxemic pigs, and also 3 strains(100.0%) among 3 enterotoxemic cattle.

Clostridium perfringens A형이 생산하는 장독소의 검색을 위한 RPLA법과 PCR기법의 감도 비교

정희곤 한국환경보건학회 1997 한국환경보건학회지 Vol.23 No.4

Clostridium perfringens A형이 생산하는 장독소를 검색해본 결과, RPLA법에 있어서는 2배로 희석한 용액으로부터 64배로 희석한 용액 (NCTC 8239 Hobbs serotype 3 CPE$^+$)에서까지 양성반응을 보였으며 PCR 기법에 있어서는 10 pg 희석 용액까지 364 bp의 장독소 DNA fragment(NCTC 8238 Hobbs serotype 2 CPE$^+$)를 확인 할수 있었다. 그러므로 장독소를 검색하기 위해서는 PCR기법이 RPLA법에 비하여 훨씬 감도가 높음을 확인할 수 있었다.