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정현미,김상종 ( Hyen Mi Chung,Sang Jong Kim ) 한국하천호수학회 1987 생태와 환경 Vol.20 No.4
Cadmium-resistant bacterium was isolated from Anyang-river on agar plate media containing 100 ppm of cadmium chloride. The characteristics of the isolated strain were consistent with that of genus Pseudomonas. It showed multi-resistant to various heavy metals including mercury, lead and copper. The effects of cadmium on the bacterial growth were measured by turbidity, viability and ³H-glucose uptake rate. In the early stage of growth, the viability and the physiological activity were rapidly decreased by the toxicity of cadmium. But after 3 hours, the bacterium began to grow exponentially and the activity became almost equal to that of the control without cadmium. The isolated strain had broad-spectrum resistance to various antibiotics such as ampicillin, chloramphenicol, kanamycin, streptomycin and tetramycin. It seemed that the resistance to cadmium is genetically controlled from the results that the isolated strain maintained high activity under high concentration of cadmium and showed multi-resistance to antibiotics.
정현미(Hyen Mi Chung),강태구(Tae Gu Kang),염철민(Cheol Min Yeom),이덕길(Duk Kil Rhee),윤제용(Je Yong Yoon) 한국물환경학회 2002 한국물환경학회지 Vol.18 No.1
This study was performed to select indicator microorganism practically applicable to water treatment plants for evaluating the removal of microorganisms such as viruses and protozoan cysts. Total of seven microorganisms were investigated. Four microorganisms for representing bacteria (E. coli, fecal coliform (FC), total coliform (TC) and heterotrophic plate counts (HPC)), two for protozoan (aerobic spore formers (ASF) and Clostridium perfringens (CP) spore) and one for viruses (F-specific RNA coliphage) were examined as a possible indicating microorganism. Each candidate microorganism was measured every month for 20 months in source and treated water of three water treatment plants (WTPs) characterized with varying water quality (lake or river; high or low BOD). The distribution of microorganisms in source and treated water were measured, and log removals of microorganisms in each water treatment plant were compared. Major findings are that ASF and HPC appeared to be the best indicator microorganism for evaluating pathogenic removal in water treatment plant although ASF was considered better. This decision was made from the following three criteria i) not only high but also stable level in the source water enough for quantitative evaluation, ii) the feasibility of detection method, iii) stability of the organisms in the water environment. For HPC and CP, the growth of microorganism and no detection in treated water were indicated as a weak point, respectively in this consideration.
환경 유형에 따른 바이오에어로졸 중 배양성 세균 동정 및 계통분석
이시원,정현미,박수정,최별,김지혜,이보람,주윤리,권오상,정원화,Lee, Siwon,Chung, Hyen-Mi,Park, Su Jeong,Choe, Byeol,Kim, Ji Hye,Lee, Bo-Ram,Joo, Youn-Lee,Kwon, Oh Sang,Jheong, Weon Hwa 한국미생물·생명공학회 2015 한국미생물·생명공학회지 Vol.43 No.2
바이오에어로졸은 0.02-100 μm 입자로, 자연 및 인위적 환경 또는 사람의 활동에 의해 발생된다. 바이오에어로졸은 바이러스, 세균, 곰팡이, 원생생물, 곰팡이 포자, 미생물 독소, 꽃가루, 동식물 기원의 물질, 타액 또는 글루칸 등으로 구성되며, 인간과 동물에게 호흡기 등의 질환을 유발한다. 본 연구에서는 농업, 축산, 하수처리장, 해변 및 청청지역의 바이오에어로졸 시료에서 분리한 배양성 미생물을 동정 및 계통분석을 수행한 결과, 우점 및 종의 구성 등 환경 유형 별 차이가 분석되었다. 한편 모든 시료에서 31 분리주가 Bacillus cereus로 동정되었고, 실내 양계장에서 Acinetobacter baumannii가 분리되었다. 또한 농업, 축산 및 해변에서 분리한 미생물 중 Domibacillus속, Chryceobacterium속, Nocardioides속 및 Comamonadaceae과에 속하는 새로운 종 또는 속이 분리되었다. Bioaerosols are comprised of particles 0.02-100 μm in size that originate in natural and artificial environments, and as a result of human activities. They consist of microorganisms including viruses, bacteria, fungi, and protozoa; fungal spores; microbial toxins; pollen; plant or animal material; expectorated liquid from humans; and glucans (peptidoglycan and β-glucan). Bioaerosols can cause respiratory and other diseases in humans and animals. In this study, bioaerosol samples acquired from agricultural sources, livestock, a sewage treatment plant, a beach, and a pristine area were analyzed to identify and phylogenetically characterize culturable microorganisms. The isolated bacteria exhibited regional differences, with different species dominating. However, Bacillus cereus was isolated in all samples, with a total of 31 strains isolated from all areas, and Acinetobacter baumannii was isolated from an indoor poultry farm. In addition, bacteria determined to be of novel genus or species of the genera Domibacillus, Chryceobacterium, Nocardioides and family Comamonadaceae were isolated from the agricultural, livestock and beach environments.
파이로시퀀싱 분석법을 이용한 주거 환경 중 거실과 화장실의 세균 특성
이시원 ( Siwon Lee ),정현미 ( Hyen Mi Chung ),박응로 ( Eung Roh Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.1
본 연구에서는 파이로시퀀싱 분석법을 이용하여 주거 환경 중 거실과 화장실의 세균 다양성을 분석하였다. 주거 환경 중 거실과 화장실에 존재하는 세균의 총 유전자량과 다양성 지수는 차이가 없었으나, 존재하는 세균의 종류에서는 차이가 나타났다. Phylum-level에서는 거실에서 나타나지 않은 Acidobacteria, Chlorobi, Chloroflexi, Fusobacteria, Nitrospirae 및 Planctomycetes문이 화장실 공기중에서 분석되어 상대적으로 넓은 영역의 세균 분포가 추정되었으며, classlevel에서는 우점하는 Proteobacteria문 중 β-Proteobacteria와 δ-Proteobacteria강이 거실에 비해 화장실에서 높은 비율로 분석되었다. 한편 genus-level에서는 거실이 화장실에 비해 상대적으로 다양한 속이 분석되었으나, 모두 Methylobacterium 속이 우점하였으며 두 주거 환경에서 각각 특징적으로 분포하는 미생물이 존재하였다. In this study, bacterial diversity in the living room and bathroom of a residential environment was analyzed using the pyrosequencing method. There was no difference in the diversity index of bacteria between the 2 rooms; however, differences were noted in the composition of bacteria. The classes β-Proteobacteria and δ-Proteobacteria were found in the bathroom at higher abundances than in the living room. The phyla Acidobacteria, Chlorobi, Chloroflexi, Fusobacteria, Nitrospirae, and Planctomycetes were found in the bathroom, but not in the living room, indicating a broader range of bacteria. However, the living room showed a more diverse range of bacterial genera than the bathroom did. In both the living room and the bathroom, the genus Methylobacterium was dominant.