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酸棗仁이 CT105에 의한 신경세포 상해 및 白鼠의 기억에 미치는 영향
정정욱,박창국,박치상,이소연,윤현덕,신오철 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1
Objective: This study was designed to research whether demonstrate neuroprotective and memory enhancing effects of Z1BU, suggesting its beneficial actions for the treatment of AD. Methods: The present study investigated effects of the dichloromethane extract of Semen Zizyphi Spinosae(ZIBU) on neurotoxicity and reactive oxygen species(ROS) and nitric oxide(NO) formation in PC 12 cell line overexpressed with carboxyl-terminal 105 amino acid peptide fragment of amyloid precursor protein(CT105). In addition, the present study evaluated its radical scavenging activity and effects on acetylcholinesterase(AChE) activity. Furthermore, effects on neuronal loss in CAl area and cognitive deficits induced by scopolamine treatment were also evaluated in rats. Result: we found in this study that ZIBU significantly inhibited apoptotic neuronal death induced by CT105 overexpression in PC12 cells, with the IC_(50) value of 23 ㎍/ml. Based on phase-contrast and fluorescence microscopic examinations, ZIBU reversed morphological changes of CT105-expressed PC-12 cells. It was also found that Z1BU significantly induced neurite outgrowth and inhibited formation of ROS and NO.Z1BU scavenged DPPH radicals and inhibited AChE activity. Furthermore, it attenuated memory impairment induced by the injection of scopolamine in rats, based on passive avoidance test. Crystal violet staining of rat brain slices exhibited increase in the viability of pyramidal neurons in hippocampal CAI area. Conclusion: these results demonstrate neuroprotective and memory enhancing effects of ZIBU, suggesting its beneficial actions for the treatment of AD.
정정욱,박정미,최병옥,김남근,오도연,정우상,Jung, Jung-Uk,Park, Jung-Mi,Choi, Byung-Ok,Kim, Nam-Keun,Oh, Do-Yeun,Jung, Woo-Sang 대한한방내과학회 2002 大韓韓方內科學會誌 Vol.23 No.1
Objective : Hyperhomocysteinemia has been proven to be an independent risk factor for stroke. The genetic mutation of methylenetetrahydrofolate reductase(MTHFR) elevates serum homocysteine level, but it still remains controversial whether the MTHFR gene mutation could be a predictor of ischemic stroke. Therefore, we studied if this genetic defect could cause ischemic stroke independently. Methods : We gathered ischemic stroke subjects and age, sex-matched controls. Age, gender, past medical history, smoking habit, serum homocysteine level, and the MTHFR genotype were recorded. General characteristics of ischemic stroke subjects were compared to the controls. We classified the stroke according to the related vessels(small and large artery infarction) and single lesion and multiple infraction. Relevant risk of the MTHFR genotype was evaluated in each stroke subtype with multiple logistic regression analysis. Results : When the controls were compared to the whole ischemic stroke, there was no specific difference except some medical histories. However, further analysis based on stroke subtypes showed important results. The small artery infarction group, multiple infraction group had significant odds ratio of the MTHFR TT genotype adjusted for age, gender, medical history and smoking habit. Conclusions : The MTHFR TT genotype is an independent risk factor for certain types of ischemic stroke, small artery infarction and multiple infarction.
보익양위탕가미방(補益養胃湯加味方)으로 6개월간(個月間) 저신장증(低身長症) 환자(患者)를 치료(治療)한 치험(治驗) 2례(例)
정정욱,양태규,구본홍,이세연,Cheong, Jeong-Ook,Yang, Tae-Kyu,Koo, Bon-Hong,Lee, Sea-Yun 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.4
IShort stature is two standard deviations (SD) below the mean for height (below the third percentile) in a population of children. We treated two cases below the third percentile for height with Boikyangwitang-gamibang(補益養胃湯加味方). During treatment Case I grew 4.2cm and became above the third percentile for height. Case II also grew 5.8cm and became above the third percentile for height. In the results We recognized Short status was showed prominent improvement with Boikyangwitang-gamibang(補益養胃湯加味方).
성장치료를 위한 한약투여의 임상적 효과 및 안전성 평가
정정욱,양태규,구본홍,이세연,Cheong, Jeong-Ook,Yang, Tae-Kyu,Koo, Bon-Hong,Lee, Sea-Yun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.4
Objective : Recently many studies of herbal medication to promote growth have been implemented. The purpose of this study was to identify the feasibility and safety of herbal medication on short stature. Methods : 143 patients(87 males, 56 Females)who visited with short stature at Kangnam CHA Oriental Hospital From February 1999 to January 2000 and were treated during 6 months, were available for this study. We treated with 5 herbal medications.(Soeumin hyangbujapalmultang-gagambang, Soyangin yukmijihwangtang-gagambang, Taeumin chungsimyunjatang-gagambang, Boa-tang, Boikyangwi tang) At the begining of the treatment and after 6 months treatment, We measured height, weight and body mass index(BMI). And we investigated changes of aspartate aminotransferase(AST), alanine aminotransferase(ALT), blood urea nitrogen(BUN), alkaline phosphatase(ALP), creatinine. Results: The distribution of ages was from 5 years old to 17 years old. After herbal treatment, mean height, mean weight, mean BMI and mean percentile grade increased $138.5{\pm}15.6$ to $141.9{\pm}15.1$, from $36.9{\pm}13.2$ to $38.9{\pm}13.2$, from $18.5{\pm}3.29$ to $18.7{\pm}3.21$, from $3.2{\pm}1.2$ to $3.4{\pm}1.2$. The contents of AST, ALT, BUN, ALP and Creatinine were not showed any significant change. Conclusions : In the results We recognized Short stature was showed prominent feasibility and safety of herbal medication. Herbal Medication in patients with short stature will improve height velocity without liver and kidney function disorders.
Pseudomonas sp. HJ-2를 이용한 고무탄성 Polyhydroxyalkanoate의 생산
정정욱,최강욱,김영백,이영하 한국미생물학회 2000 미생물학회지 Vol.36 No.2
Pseudomonas sp. HJ-2는 heptanoinc acid를 단일탄소원으로 이용하여 3-hydroxybutyrate (3HB),3-hydroxy-velerate (3HV) 및 3-hydroxybutyrate (3HHp)를 구성 단위체로 하는 고무탄성 polyhydroxyalkanoate (PHA)를 생합성한다. 이 미생물 고분자는 poly(3HB-co-3HV)공중합체와 poly(3HHp) 단일중합체로 이루어진 혼합물임이 밝혀졌다. 본 연구에서는 PHA가 고무탄성체로서의 성질을 유지하는데 필용한 단위체 조성과 HJ-2의 배양조건이 PHA의 생산 및 단위체 조성에 미치는 영향에 대하여 조사하였다. 생합성된 PHA의 탄성률은 poly(3HHp)의 존재로 크게 감소되었으나, 3HV의 함량이 높은 poly(3HB-co-3HV) 자체도 최대변형률 740%로서 고무탄성체로서의 성질을 보였다. HJ-2의 생장 및 PHA 생합성은 탄소원인 heptanoic acid의 초기농도가 40mMdlfEo 가장 높았으나, 50mM의 농도에서는 큰 저해를 받았다. PHA 생합성은 질소와 인이 결핍된 조건에서 크게 증가되었다. 배양액의 pH 및 통기는 HJ-2로부터 생합성되는 PHA의 단위체 조성에 큰 영향을 주는 것으로 나타났다. pH 7.5에서 생합성되는 고분자는 poly(3HB-co-38% 3HV)인 반면에 pH8.0에서의 고분자는 3HHp가 95%를 차지하였다. 발효조의 교반속도를 달리한 실험의 결과 고분자 내 3HHp의 함량은 산소전달 속도가 높아질수록 증가하였다. Pseudomnas sp. HJ-2 is capable of producing a rubber-elastic polyhydroxyalkanoate (PHA) consisting of 3- hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyheptanoate (3HHp) from heptanoic acid as the sole carbon source. The polyester produced was a blend of poly(3HB-co-3HV) and poly(3HHp). Although the mixing of poly(3HHp) fraction to poly(3HB-co-3HV) resulted in a decrease of modulus, the sole fraction of poly(3HB-co-3HV) with a high molar fraction of 3HV was shown to be an elastomer with the maximum percent strain of 740%. The biomass yield and the PHA synthesis were relatively high when the initial heptanoic acid concentration was 40 mM, and were significantly decreased when the substrate concentration exceeded 50 mM. The accumulation of PHA was stimulated by deficiency of nitrogen and phosphorus in the medium. The PHA contents and its monomeric compositions were greatly affected by pH and oxygen transfer rate. At pH 7.5, poly(3HB-~0.38% 3HV) was produced from heptanoic acid and a mixture of 95% 3HHp and 5% 3HV was produced at pH 8.0. Increased conten1 of 3HHp in the polyesters with lhe increasing oxygen transfer rate by agitation speed a1 a fixed aeration rate was observed.
자연과학편 : SNP T45G 유전자 다형성에 따른 심폐체력과 대사증후군 지표와의 관계
정정욱(JeongWookJung),김태경(TaeKyungKim),강현식(HyunSikKang) 한국체육학회 2009 한국체육학회지 Vol.48 No.2
아디포넥틴은 지방조직에서 분비되는 특수한 단백질로서 비만이나 대사증후군 위험요소, 만성질환 및 제 2형 당뇨환자에게서 감소하는 것으로 알려져 있다. 본 연구는 20대 성인을 대상으로 한국인의 아디포넥틴 유전자 다형성(SNP T45G)을 분석하여 다형에 따른 대사증후군 및 심폐체력과의 연관성을 알아보는데 그 목적이 있다. 한국인 20대 성인 700명(남자 497명, 여자 203명)을 대상으로 Real-Time PCR을 이용하여 SNP T45G를 분석하였으며, 심폐체력을 측정하기 위하여 GXT를 통해 최대산소섭취량을 측정하였다. 또한 신체구성(신체질량지수, 체지방량, 허리둘레)과 혈액변인(지질, 공복시 글루코스, 공복시 인슐린, 아디포넥틴)을 표준화된 프로토콜에 따라 측정하였다. 그 결과 SNP T45G의 G 대립유전자가 T 대립유전자 보다 BMI(p=.027)가 높게 나타났으며, G 대립유전자에서 심폐체력이 낮은 집단의 허리둘레(P<.001), BMI(P<.001), %BF(P<.001), SBP(P=.010), DBP(P=.006), 공복시 혈당(P=.001), TG(P<.001), HDL-C(P=.022)과 LDL-C(P=.004)에서 심폐체력이 높은 집단과 유의한 차이를 보였다. 한국인의 SNP T45G 유전자 다형은 대사증후군 지표와 비만지표로 의미가 있을 것으로 생각되며, SNP T45G의 위험인자는 G 대립 유전자로 생각된다. 또한 환경적인 요소인 심폐체력이 정상보다 낮을 경우 젊은 한국 성인의 대사증후군과 비만에 부정적인 영향을 미치는 것으로 생각된다. Adiponectin is an adipose tissue specific protein that is decreased in subjects with obesity, metabolic risk factors, and chronic diseases including type 2 diabetes. The purpose of the study was to investigate whether or not the SNP T45G gene single nucleotide polymorphisms were associated with cardiorespiratory fitness (CRF) and metabolic syndrome markers in their twenties. The SNP T45G were genotype in young 700 korean adults (497 men and 203 women) by using the Real-Time Quantitative PCR with TaqMan detection chemistry, CRF was measured as the maximum volume of minute oxygen consumption during a graded treadmill testing. Body composition (i.e., body mass index, percent body fat, waist circumference) and blood chemistry (lipids, glucose, insulin, adiponectin) were measured using our standardized protocols. With respect to SNP T45G variants, group analyses showed that those with G allele had significantly higher BMI(P=.027) than those with T allele. Further, those with low CRF had significantly higher waist circumference(P<.001), BMI(P<.001), %BF(P<.001), SBP(P=.010), DBP(P=.006), fasting glucose(P=.001), TG(P<.001), HDL-C(P=.022) and LDL-C(P=.004) than those with high CRF in G allele. The current findings of the study suggest that 1) the adiponectin gene SNP T45G polymorphism may be used as a genetic susceptibility to obesity and metabolic syndrome, risk allele is G allele. 2) Row CRF may play as an important modulator in determining the significance of the genetic predisposition to obesity and metabolic syndrome in young Korean adults.