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Lentinus edodes 에서 분비되는 Laccase 의 특성
정인범,윤홍덕,맹진수,강사욱,하영칠,정가진,최형태,김재헌 한국미생물학회 1992 미생물학회지 Vol.30 No.4
Lentinus edodes 에서 분비된 laccase 를 DEAE Sephadex A-50, Con A-Sepharose, Sephadex G-150 크로마토그래피를 통해서 순수분리하였다. 효소는 분자량이 87 KDa 정도인 하나의 소단위체로 되어 있고, 12.0% 의 당을 함유하고 있었다. 그리고 N-말단 아미노산 서열은 Pleurotus oxtreatus 와 Cloriolus hirsutus 의 laccase 와 유사하였다. 효소의 최적 pH 는 4.8 이고 최적 온도는 $40^{\circ}C$ 이었다. 그리고 본 효소는 pH 7-9 와 $30^{\circ}C$이하에서 비교적 안정하였다. Syringaldazine 에 대한 $K_{M}$ 은 $0.4\mu$ M 이었고 $k_{cat}$ 은$ 77 sec^{-1}$ 이었다. This layer chromatography 에 의한 본 효소의 반응 산물들의 분리양상이 Pleurotus ostreatus 의 laccase 의 경우와 유사하였다. Extracellular laccase excreted from Lentinus edodes ATCC 48085 was purified through a series of DEAF, Sephadex A-50. Con A-Sepharosc and Sephadex G-150 chromatography. Extracellular enzyme. which consists of a single polypeptide, has a n~olecular mass of 87.000 daltons and contains 12.0'%, carbohydrate. The N-terminal amino acid sequence (I5 residues) of the puritied enzyme was similar to that of laccases of PIeurotus ostreatus and Coriolus hirsutus. The enzyme showed optimal activity at near pH 4.8 and $40^{\circ}C$. The enzyme was stable at pH 7-9 and below $30^{\circ}C$. $K_{M}$ and $k_{cat}$ values for syringaldazine were estimated to be $0.4\mu\textrm{M}$ and 77 sec, respectively. The developed patterns of reaction products of thevenzyme on thin layer chromatography were similar to those of laccase of Pleurotus ostreatus.
정인범,오재성,이승환,장인진,이영조,정재용 대한임상약리학회 2017 Translational and Clinical Pharmacology Vol.25 No.4
Because bioequivalence studies are performed using a crossover design, information on the intrasubjectcoefficient of variation (intra-CV) for pharmacokinetic measures is needed when determiningthe sample size. However, calculated intra-CVs based on bioequivalence results of identicalgeneric drugs produce different estimates. In this study, we collected bioequivalence results usingpublic resources from the Ministry of Food and Drug Safety (MFDS) and calculated the intra-CVs of various generics. For the generics with multiple bioequivalence results, pooled intra-CVswere calculated. The estimated intra-CVs of 142 bioequivalence studies were 14.7±8.2% for AUCand 21.7±8.8% for Cmax. Intra-CVs of Cmax were larger than those of area under the concentrationtimecurve (AUC) in 129 studies (90.8%). For the 26 generics with multiple bioequivalence results,the coefficients of variation of intra-CVs between identical generics (mean±sd (min ~ max)) were38.0±24.4% (1.9 ~ 105.3%) for AUC and 27.9±18.2 % (4.0 ~ 70.1%) for Cmax. These results suggestthat substantial variation exists among the bioequivalence results of identical generics. In this study,we presented the intra-CVs of various generics with their pooled intra-CVs. The estimated intra-CVs calculated in this study will provide useful information for planning future bioequivalencestudies.